MEG3 (AK055725) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FP504; GTL2; LINC00023; NCRNA00023; onco-lncRNA-83; prebp1; PRO0518; PRO2160
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK055725, the custom clone sequence may differ by one or more nucleotides ATGAGAAGACTTTCAATAGTAATGAAGAATCCATGGCACTCTCCTCACCCTCAAACACAT GGCAGTCATTCACATACAGGCCCCAAAGCCACTGTTAGTGCTGCAGTAGCTCCTGTGGAC ATTGGAAAGCCCGGAGAGGGCGTGGAAGAAATCAGCTGGCCCCCGGCAGGTTCTCTGGGG TTTTGTGCCCAAGGCTCCTGGAGCCCTAAAAACTTTCAAAAGTTAACTCCCCACGTCCCC ATCCTGCTTGGGTTTCTGGACTTTTCTGAGGCACCGGCAGAGGGGTCTCGTTGCTCCCTT GAGTGTAGGGGCAGCCCTTTAACCTGGCTCCTTGAGTCCCTGCTTTTTCTGCTTCTGTTG CCTTCTTCCTCGTCTTCCTCTCTCTCAATATCTCCCTCTCTTTGTCCCTCCCCAGTTCCT GACCTGGCCATCCCGGGGTGCCCT
Restriction Sites	Please inquire
ACCN	AK055725
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK055725.1, BAB70996.1
RefSeq Size	2861 bp
RefSeq ORF	447 bp
Locus ID	55384
Gene Summary	This gene is a maternally expressed imprinted gene. Multiple alternatively spliced transcript variants have been transcribed from this gene and all of them are long non-coding RNAs (lncRNAs). This gene is expressed in many normal tissues, but its expression is lost in multiple cancer cell lines of various tissue origins. It inhibits tumor cell proliferation in vitro. It also interacts with the tumor suppressor p53, and regulates p53 target gene expression. Its deletion enhances angiogenesis in vivo. Many experimental evidences demonstrate that this gene is a lncRNA tumor suppressor. [provided by RefSeq, Mar 2012]

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