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PRKAG2 (NM_001040633) Human Untagged Clone
CAT＃: SC311157
PRKAG2 (untagged)-Human protein kinase, AMP-activated, gamma 2 non-catalytic subunit (PRKAG2), transcript variant c
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AAKG; AAKG2; CMH6; H91620p; WPWS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC311157 representing NM_001040633.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCGCTCCTGGACGGAGACCTGGAGGGTTCCGGAAAGCATTCCTCTCGAAAGGTGGACAGCCCCTTC GGCCCGGGCAGCCCCTCCAAAGGGTTCTTCTCCAGAGGCCCCCAGCCCCGGCCCTCCAGCCCCATGTCT GCACCTGTGAGGCCCAAGACCAGCCCCGGCTCTCCCAAAACCGTGTTCCCGTTCTCCTACCAGGAGTCC CCGCCACGCTCCCCTCGACGCATGAGCTTCAGTGGGATCTTCCGCTCCTCCTCCAAAGAGTCTTCCCCC AACTCCAACCCTGCTACCTCGCCCGGGGGCATCAGGTTTTTCTCCCGCTCCAGAAAAACCTCCGGCCTC TCCTCCTCTCCGTCAACACCCACCCAAGTGACCAAGCAGCACACGTTTCCCCTGGAATCCTATAAGCAC GAGCCTGAACGGTTAGAGAATCGCATCTATGCCTCGTCTTCCCCCCCGGACACAGGGCAGAGGTTCTGC CCGTCTTCCTTCCAGAGCCCGACCAGGCCTCCACTGGCATCACCGACACACTATGCTCCCTCCAAAGCC GCGGCGCTGGCGGCGGCCCTGGGACCCGCGGAAGCCGGCATGCTGGAGAAGCTGGAGTTCGAGGACGAA GCAGTAGAAGACTCAGAAAGTGGTGTTTACATGCGATTCATGAGGTCACACAAGTGTTATGACATCGTT CCAACCAGTTCAAAGCTTGTTGTCTTTGATACTACATTACAAGTTAAAAAGGCCTTCTTTGCTTTGGTA GCCAACGGTGTCCGAGCAGCGCCACTGTGGGAGAGTAAAAAACAAAGTTTTGTAGGAATGCTAACAATT ACAGATTTCATAAATATACTACATAGATACTATAAATCACCTATGGTACAGATTTATGAATTAGAGGAA CATAAAATTGAAACATGGAGGGAGCTTTATTTACAAGAAACATTTAAGCCTTTAGTGAATATATCTCCA GATGCAAGCCTCTTCGATGCTGTATACTCCTTGATCAAAAATAAAATCCACAGATTGCCCGTTATTGAC CCTATCAGTGGGAATGCACTTTATATACTTACCCACAAAAGAATCCTCAAGTTCCTCCAGCTTTTTATG TCTGATATGCCAAAGCCTGCCTTCATGAAGCAGAACCTGGATGAGCTTGGAATAGGAACGTACCACAAC ATTGCCTTCATACATCCAGACACTCCCATCATCAAAGCCTTGAACATATTTGTGGAAAGACGAATATCA GCTCTGCCTGTTGTGGATGAGTCAGGAAAAGTTGTAGATATTTATTCCAAATTTGATGTAATTAATCTT GCTGCTGAGAAAACATACAATAACCTAGATATCACGGTGACCCAGGCCCTTCAGCACCGTTCACAGTAT TTTGAAGGTGTTGTGAAGTGCAATAAGCTGGAAATACTGGAGACCATCGTGGACAGAATAGTAAGAGCT GAGGTCCATCGGCTGGTGGTGGTAAATGAAGCAGATAGTATTGTGGGTATTATTTCCCTGTCGGACATT CTGCAAGCCCTGATCCTCACACCAGCAGGTGCCAAACAAAAGGAGACAGAAACGGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001040633 |
| Insert Size | 1578 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001040633.1 |
| RefSeq Size | 3154 bp |
| RefSeq ORF | 1578 bp |
| Locus ID | 51422 |
| UniProt ID | Q9UGJ0 |
| Protein Families | Druggable Genome |
| Protein Pathways | Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway |
| MW | 58.4 kDa |
| Gene Summary | AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jan 2015] Transcript Variant: This variant (c) contains an alternate 5' exon and the translation starts from a downstream AUG codon, compared to variant a. The resulting isoform (c) has a shorter N-terminus when compared to isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216876 | PRKAG2 (Myc-DDK-tagged)-Human protein kinase, AMP-activated, gamma 2 non-catalytic subunit (PRKAG2), transcript variant c |
CNY 5,864.00 |
|
| RC216876L1 | Lenti ORF clone of Human protein kinase, AMP-activated, gamma 2 non-catalytic subunit (PRKAG2), transcript variant c, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216876L2 | Lenti ORF clone of Human protein kinase, AMP-activated, gamma 2 non-catalytic subunit (PRKAG2), transcript variant c, mGFP tagged |
CNY 8,264.00 |
|
| RC216876L3 | Lenti ORF clone of Human protein kinase, AMP-activated, gamma 2 non-catalytic subunit (PRKAG2), transcript variant c, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216876L4 | Lenti ORF clone of Human protein kinase, AMP-activated, gamma 2 non-catalytic subunit (PRKAG2), transcript variant c, mGFP tagged |
CNY 5,890.00 |
|
| RG216876 | PRKAG2 (tGFP-tagged) - Human protein kinase, AMP-activated, gamma 2 non-catalytic subunit (PRKAG2), transcript variant c |
CNY 7,464.00 |
