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NRF1 (NM_001040110) Human Untagged Clone
CAT＃: SC310917
NRF1 (untagged)-Human nuclear respiratory factor 1 (NRF1), transcript variant 2
CNY 6,000.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ALPHA-PAL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001040110 |
| Insert Size | 1512 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001040110.1 |
| RefSeq Size | 3523 bp |
| RefSeq ORF | 1512 bp |
| Locus ID | 4899 |
| UniProt ID | Q16656 |
| Protein Families | Transcription Factors |
| Protein Pathways | Huntington's disease |
| MW | 53.5 kDa |
| Gene Summary | This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the 3' coding region, compared to variant 3. The encoded protein (isoform 1) is shorter, compared to isoform 2. Variants 1 and 2 encode the same protein (isoform 1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217852 | NRF1 (Myc-DDK-tagged)-Human nuclear respiratory factor 1 (NRF1), transcript variant 2 |
CNY 5,616.00 |
|
| RC217852L1 | Lenti ORF clone of Human nuclear respiratory factor 1 (NRF1), transcript variant 2, Myc-DDK-tagged |
CNY 8,016.00 |
|
| RC217852L2 | Lenti ORF clone of Human nuclear respiratory factor 1 (NRF1), transcript variant 2, mGFP tagged |
CNY 8,016.00 |
|
| RC217852L3 | Lenti ORF clone of Human nuclear respiratory factor 1 (NRF1), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC217852L4 | Lenti ORF clone of Human nuclear respiratory factor 1 (NRF1), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG217852 | NRF1 (tGFP-tagged) - Human nuclear respiratory factor 1 (NRF1), transcript variant 2 |
CNY 7,216.00 |
