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TNNT3 (NM_006757) Human Untagged Clone
CAT＃: SC310590
TNNT3 (untagged)-Human troponin T type 3 (skeletal, fast) (TNNT3), transcript variant 1
CNY 2,400.00
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | beta-TnTF; DA2B2; TNTF |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_006757 edited
GAACCAGCTGGGAGGACAGTACGAGGGGCTGGTGGGCAGCCTGGGACTGGGTCACACCGG GGGCCTGGACAACACTGGCTCGTGGGGCTGAGGACTGAGGAGAGCCTGGCCCAGCCGTGG CAGTTACAAGAGGCTTAACCTGAGGGGCAGCAGAGTCTCAGGAAGAGATTCTGGGATAGG AAAAAGCTACCCCCGTTGCCTGGAAGACAGCTATCTGCTGGAAACCACCCACCTTCACCA TGTCTGACGAGGAAGTTGAACAGGTGGAGGAGCAGTACGAAGAAGAAGAGGAAGCCCAGG AGGAAGAGGAAGTTCAAGAAGACACCGCAGAGGAGGACGCGGAAGAGGAGAAACCGAGAC CCAAACTCACTGCTCCTAAGATCCCAGAAGGGGAGAAAGTGGACTTCGATGACATCCAGA AGAAGCGTCAGAACAAAGACCTAATGGAGCTCCAGGCCCTCATCGACAGCCACTTTGAAG CCCGGAAGAAGGAGGAGGAGGAGCTGGTCGCTCTCAAAGAGAGAATCGAGAAGCGCCGTG CAGAGAGAGCGGAGCAGCAGAGGATTCGTGCAGAGAAGGAGAGGGAGCGCCAGAACAGAC TGGCGGAGGAAAAGGCCAGAAGGGAGGAGGAGGATGCCAAGAGGAGGGCAGAGGACGACC TGAAGAAGAAGAAAGCTCTGTCTTCCATGGGAGCCAACTACAGCAGCTACCTGGCCAAGG CTGACCAGAAGAGAGGCAAGAAGCAGACAGCCCGGGAAATGAAGAAGAAGATTCTGGCTG AGAGACGCAAGCCGCTCAACATCGATCACCTTGGTGAAGACAAACTGAGGGACAAGGCCA AGGAGCTCTGGGAGACCCTGCACCAGCTGGAGATTGACAAGTTCGAGTTTGGGGAGAAGC TGAAACGCCAGAAATATGACATCATGAATGTCCGGGCCAGAGTGCAGATGCTGGCCAAGT TCAGCAAGAAGGCTGGGACCCCAGCCAAGGGCAAAGTCGGCGGGCGCTGGAAGTAGAGAG GCCAGAAAGGCCCCTCGAGGCAGAGACCCTCCGCCCTCTTGCACACCAGGGCCGCTCGTG GGACTCCACATCCTCCAGCCCCCACAATCCTGTCAGGGGCTCCCTGACAGTCCTGGGGGT GGAGAGGCCATCCCGGGGCGTCCCCCGCGTCTGTGTCCTTGCTGCCTTCATCCCCTGGGG CCTGTGAATAAAGCTGCAGAACCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAC >OriGene 5' read for NM_006757 unedited
GGGCGTTCAGATTTTGTAATACGACTTCACTATAGGGCGGCACGCGCAATTCGGCACGAG GGAACCAGCTGGGAGGACAGTACGAGGGGCTGGTGGGCAGCCTGGGACTGGGTCACACCG GGGGCCTGGACAACACTGGCTCGTGGGGCTGAGGACTGAGGAGAGCCTGGCCCAGCCGTG GCAGTTACAAGAGGCTTAACCTGAGGGGCAGCAGAGTCTCAGGAAGAGATTCTGGGATAG GAAAAAGCTACCCCCGTTGCCTGGAAGACAGCTATCTGCTGGAAACCACCCACCTTCACC ATGTCTGACGAGGAAGTTGAACAGGTGGAGGAGCAGTACGAAGAAGAAGAGGAAGCCCAG GAGGAAGAGGAAGTTCAAGAAGACACCGCAGAGGAGGACGCGGAAGAGGAGAAACCGAGA CCCAAACTCACTGCTCCTAAGATCCCAGAAGGGGAGAAAGTGGACTTCGATGACATCCAG AAGAAGCGTCAGAACAAAGACCTAATGGAGCTCCAGGCCCTCATCGACAGCCACTTTGAA GCCCGGAAGAAGGAGGAGGAGGAGCTGGTCGCTCTCAAAGAGAGAATCGAGAAGCGCCGT GCAGAGAGAGCGGAGCAGCAGAGGATTCGTGCAGAGAAGGAGAGGGAGCGCCAGAACAGA CTGGCGGAGGAAAAGGCCAGAAAGGAGGAGGAGGATGCCAAGAGGAGGGCAGAGGACGAC CTGAAGAAGAAGAAGCTCTGTCTTCCATGGGAGCCAACTACAGCAGCTACCTGGCCAAGG CTGACCAGAAGAGAGGCAAGAAGCAGACAGCCCGGGAAATGAAGAAGAAGATTCTGGCTG AGAGACGCAAGCCGCTCAACATCGATCACCTTTGGTGAAGACAAACTGAGGGACAAGGCC >OriGene 3' read for NM_006757 unedited
NNGGGTTTTGACTAGNAACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTGGGGGTTCTGCAGCTTTATTCACAGGCCCCAAGGGATGA AAGCAGCAAGGACACAAACCCCGGGGACGCCCCGGGATGGCCTCTCCACCCCCAGGACTG TCAAGGAGCCCCTGACAGGATTGTGGGGGCTGGAGGATGTGGAGTCCCACGAACGGCCCT GGTGTGCAAAAAGGCGGAGGGTCTCTGCCTCGAGGGGCCTTTTTGGCCTCTCTACTTTCA ACGCCCGCCGACTTTGCCCTTGGCTGGGGTCCCAACCTTCTTGCTGAACTTGGCCAACAT CTGCACTCTGGCCCGGACATTCATGAAGTCATATTTCTGGCGTTTCAACTTCTCCCCAAA CTCGAACTTGTCAATCTCCAACTGAGGCAAGGTCTCCCAAAACTCCTTGGCCTTGTCCCT CAATTTGTCTTCACCAAAGTGATCCATGTTGAACGGCTTGCGTCTCTTAACCAGAATCTT CTTCTTTATTTCCCGGGCTGTCTGCTTCTTGCCTCTCTTCTGGTCAACCTTGGCCAAGTA ACTGCTGTAATTGGCTCCCATGGAAGACAGAACTTTCTTCTTCTTCAAGTCGTCCTCTGG CCTCCTCTTGGCATCCTCCTCCTCCCTTCTGGCCTTTTCCTCCGCCAGTCTGTTCTGGCG CTCCCTCTCCTTCTCTGCACGAATCCTCTGGTGGTCCGCTCTCTCTGGACGGCGCTTCTC GATTCTCTCTTTGAGAGCGAACAACTTCTTCTCCTCCTTCTTTCGGGCTTTAAAATGGCT GTCGATGAAGGCCTGGGAGCTCCATTAAGTCTTTGGTCTGACGCTTCTTCTTGG |
| Restriction Sites | Please inquire |
| ACCN | NM_006757 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | There are 26 nucleotide differences between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 10 amino acids. There is another published reference that contains an exact match to the sequence discrepancies, which supports the existence of these polymorphisms. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006757.1, NP_006748.1 |
| RefSeq Size | 1000 bp |
| RefSeq ORF | 777 bp |
| Locus ID | 7140 |
| UniProt ID | P45378 |
| Domains | Troponin |
| Gene Summary | The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B). [provided by RefSeq, Oct 2009] Transcript Variant: This variant (1) encodes isoform 1. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216642 | TNNT3 (Myc-DDK-tagged)-Human troponin T type 3 (skeletal, fast) (TNNT3), transcript variant 1 |
CNY 2,400.00 |
|
| RC216642L1 | Lenti-ORF clone of TNNT3 (Myc-DDK-tagged)-Human troponin T type 3 (skeletal, fast) (TNNT3), transcript variant 1 |
CNY 4,800.00 |
|
| RC216642L2 | Lenti-ORF clone of TNNT3 (mGFP-tagged)-Human troponin T type 3 (skeletal, fast) (TNNT3), transcript variant 1 |
CNY 5,890.00 |
|
| RC216642L3 | Lenti-ORF clone of TNNT3 (Myc-DDK-tagged)-Human troponin T type 3 (skeletal, fast) (TNNT3), transcript variant 1 |
CNY 5,890.00 |
|
| RC216642L4 | Lenti-ORF clone of TNNT3 (mGFP-tagged)-Human troponin T type 3 (skeletal, fast) (TNNT3), transcript variant 1 |
CNY 5,890.00 |
|
| RG216642 | TNNT3 (tGFP-tagged) - Human troponin T type 3 (skeletal, fast) (TNNT3), transcript variant 1 |
CNY 4,000.00 |
