PSME3 (NM_176863) Human Untagged Clone
CAT#: SC310584
PSME3 (untagged)-Human proteasome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki) (PSME3), transcript variant 2
CNY 2,400.00
CNY 6,270.00
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | HEL-S-283; Ki; PA28-gamma; PA28G; PA28gamma; REG-GAMMA |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF sequence for NM_176863 edited
ATGGCCTCGTTGCTGAAGGTGGATCAGGAAGTGAAGCTCAAGGTTGATTCTTTCAGGGAG CGGATCACAAGTGAGGCAGAAGACTTGGTGGCAAATTTTTTCCCAAAGAAGTTATTAGAA CTTGATAGTTTTCTGAAGGAACCAATCTTAAACATCCATGACCTAACTCAGATCCACTCT GACATGAATCTCCCAGTCCCTGACCCCATTCTTCTCACCAATAGCCATGATGGACTGGAT GGTCCCACTTATAAGAAGCGAAGGTTGGATGAGTGTGAAGAAGCCTTCCAAGGAACCAAG GTGTTTGTGATGCCCAATGGGATGCTGAAAAGCAACCAGCAGCTGGTGGACATTATTGAG AAAGTGAAACCTGAGATCCGGCTGTTGATTGAGAAATGTAACACGCCTTCAGGCAAAGGT CCTCATATATGTTTTGACCTCCAGGTCAAAATGTGGGTACAGCTCCTGATTCCCAGGATA GAAGATGGAAACAACTTTGGGGTGTCCATTCAGGAGGAAACAGTTGCAGAGCTAAGAACT GTTGAGAGTGAAGCTGCATCTTATCTGGACCAGATTTCTAGATATTATATTACAAGAGCC AAATTGGTTTCTAAAATAGCTAAATATCCCCATGTGGAGGACTATCGCCGCACCGTGACA GAGATTGATGAGAAAGAATATATCAGCCTTCGGCTCATCATATCAGAGCTGAGGAATCAA TATGTCACTCTACATGACATGATCCTGAAAAATATCGAGAAGATCAAACGGCCCCGGAGC AGCAATGCAGAGACTCTGTACTGA >OriGene 5' read for NM_176863 unedited
TGCGTTTGTATACGACTCCTATAGGGCGGCCGCGTAATTCGGCACCAGGAGCGAGCGAGC AGTGAGTAAGCCAGCAAGGGCGGTCGGGTCCCGAGGTCAGCCGAGATTTCTCAGGTCCCT CCGGCCCCCTCCCTGGAGTCCACAGCGCCTCCGGTGTCCAGAGGATCGGACACGGCCCGG CCCGGCCATGGCCTCGTTGCTGAAGGTGGATCAGGAAGTGAAGCTCAAGGTTGATTCTTT CAGGGAGCGGATCACAAGTGAGGCAGAAGACTTGGTGGCAAATTTTTTCCCAAAGAAGTT ATTAGAACTTGATAGTTTTCTGAAGGAACCAATCTTAAACATCCATGACCTAACTCAGAT CCACTCTGACATGAATCTCCCAGTCCCTGACCCCATTCTTCTCACCAATAGCCATGATGG ACTGGATGGTCCCACTTATAAGAAGCGAAGGTTGGATGAGTGTGAAGAAGCCTTCCAAGG AACCAAGGTGTTTGTGATGCCCAATGGGATGCTGAAAAGCAACCAGCAGCTGGTGGACAT TATTGAGAAAGTGAAACCTGAGATCCGGCTGTTGATTGAGAAATGTAACACGCCTTCAGG CAAAGGTCCTCATATATGTTTTGACCTCCAGGTCAAAATGTGGGTACAGCTCCTGATTCC CAGGATAGAAGATGGAAACAACTTTGGGGTGTCCATTCAGGAGGAAACAGTTGCAGAGCT AAGAACTGTTGAGAGTGAAGCTGCATCTTATCTGGACCAGATTTCTAGATATTATATTAC AAGAGCCAAATTGGTTTCTAAATAGCTAAATATCCCCATGTGGAGGACTATCGCCGCACC GTGACAGAGATTGATGAGAAAGAATATATCAGCCCTTCGCTCATCATATCAGAGCTGAGA TCAATATGTCACCTCTACATGAACATGATCCTGAAAAATTATCGGAGAGGATC >Forward primer walk for NM_176863 unedited
TGTTCGAAGAGAACAGGAAAATCCCAGGATAGAAGATGGAAACAACTTTGGGGTGTCCAT TCAGGAGGAAACAGTTGCAGAGCTAAGAACTGTTGAGAGTGAAGCTGCATCTTATCTGGA CCAGATTTCTAGATATTATATTACAAGAGCCAAATTGGTTTCTAAAATAGCTAAATATCC CCATGTGGAGGACTATCGCCGCACCGTGACAGAGATTGATGAGAAAGAATATATCAGCCT TCGGCTCATCATATCAGAGCTGAGGAATCAATATGTCACTCTACATGACATGATCCTGAA AAATATCGAGAAGATCAAACGGCCCCGGAGCAGCAATGCAGAGACTCTGTACTGAGGCCA GGGCCAGGGCCAGGGGACTCTGTGAGTCTGGCTCAAGACCGACATTGCCTTGGTTTGTTA CATGACTATCGTGATGGGGAAACTGGCTGGAAATAGTAATCACACCTCTCTGTTTTTAGT TAGAGTCTAATGAAACTCTCATCTAGTTCTGTGATGTGTTTACCTCTTTTTTCAGGCCTC AGGAACTCTTCTATTTCCTTCCCTAATACCCCACACCCAACCTGTCGTAATTTCTGGAGA ACTCCAGGTTTGTGTGTGCAGGATGTTGGCACAAAAATACCTGTGTTTTCATTCTCCCCC TCTCTCCCTCCTGTGTCTTGCGCTTTATGTTTTCTTCCGTTTGATAATTAGTTGGTTAAA AGCTGAGGGAACCCGGAAGGAAAGTGCTAGGTGTTTTTTAGGAACTAGGGTGGCGGGGGG ACGAACTTCTCTTCCTCACATGAGGTTACTGTTTCTTTCCTCTGGTGGGGCATTGGATCC TCCCCACAGTTGCCCTGGTGATGACTTAGGCTTTCCAATCTGTGTACATCCCACTTTGAA TCTTGATCGGTGGACAAGAAAATAACCTTAGGCCTTTCAGTC |
Restriction Sites | Please inquire |
ACCN | NM_176863 |
Insert Size | 2500 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_176863.1, NP_789839.1 |
RefSeq Size | 3228 bp |
RefSeq ORF | 804 bp |
Locus ID | 10197 |
UniProt ID | P61289 |
Protein Families | Stem cell - Pluripotency |
Protein Pathways | Antigen processing and presentation, Proteasome |
Gene Summary | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012] Transcript Variant: This variant (2), uses ans alternate in-frame splice site, compared to variant 1. The resulting isoform (2) is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222312 | PSME3 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki) (PSME3), transcript variant 2 |
CNY 3,600.00 |
|
RC222312L1 | Lenti ORF clone of Human proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki) (PSME3), transcript variant 2, Myc-DDK-tagged |
CNY 6,000.00 |
|
RC222312L2 | Lenti ORF clone of Human proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki) (PSME3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC222312L3 | Lenti ORF clone of Human proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki) (PSME3), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC222312L4 | Lenti ORF clone of Human proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki) (PSME3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG222312 | PSME3 (tGFP-tagged) - Human proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki) (PSME3), transcript variant 2 |
CNY 5,200.00 |