Acrosin (ACR) (NM_001097) Human Untagged Clone
CNY 6,750.00
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC310411 representing NM_001097.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTTGAGATGCTACCAACTGCCATTCTGCTGGTCTTGGCAGTGTCCGTGGTTGCTAAAGATAACGCC ACGTGTGATGGCCCCTGTGGGTTACGGTTCAGGCAAAACCCACAGGGTGGTGTCCGCATCGTCGGCGGG AAGGCTGCACAGCATGGGGCCTGGCCCTGGATGGTCAGCCTCCAGATCTTCACGTACAACAGCCACAGG TACCACACATGTGGAGGCAGCTTGCTGAATTCACGATGGGTGCTCACTGCTGCTCACTGCTTCGTCGGC AAAAATAATGTGCATGACTGGAGACTGGTTTTCGGAGCAAAGGAAATTACATATGGGAACAATAAACCA GTAAAGGCGCCTCTGCAAGAGAGATATGTGGAGAAAATCATCATTCATGAAAAATACAACTCTGCGACA GAGGGAAATGACATTGCCCTCGTGGAGATCACCCCTCCCATTTCGTGTGGGCGCTTCATTGGGCCGGGC TGCCTGCCCCACTTTAAGGCAGGCCTCCCCAGAGGCTCCCAGAGCTGCTGGGTGGCCGGCTGGGGATAT ATAGAAGAGAAAGCCCCCAGGCCATCATCTATACTGATGGAGGCACGTGTGGATCTCATCGACCTGGAC TTGTGTAACTCGACCCAGTGGTACAATGGGCGCGTTCAGCCAACCAATGTGTGCGCGGGGTATCCTGTA GGCAAGATCGACACCTGCCAGGGAGACAGCGGCGGGCCTCTCATGTGCAAAGACAGCAAGGAAAGCGCC TATGTGGTCGTGGGAATCACAAGCTGGGGGGTAGGCTGTGCCCGTGCCAAGCGCCCCGGAATCTACACG GCCACCTGGCCCTATCTGAACTGGATCGCCTCCAAGATTGGTTCTAACGCTTTGCGTATGATTCAATCG GCCACCCCTCCACCTCCCACCACTCGACCGCCCCCGATTCGACCCCCCTTCTCCCACCCTATCTCTGCT CACCTTCCTTGGTATTTCCAACCGCCCCCTCGACCACTTCCACCCCGACCACCGGCAGCCCAGCCCCGA CCCCCACCTTCACCCCCGCCCCCACCCCCACCTCCAGCCTCACCTTTACCCCCACCCCCACCCCCACCC CCACCTACACCCTCATCTACCACAAAACTTCCCCAAGGACTTTCTTTTGCCAAGCGCCTACAGCAGCTC ATAGAGGTCTTGAAGGGGAAGACCTATTCCGACGGAAAGAACCATTATGACATGGAGACCACAGAGCTC CCAGAACTGACCTCGACCTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_001097 |
Insert Size | 1266 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001097.2 |
RefSeq Size | 1384 bp |
RefSeq ORF | 1266 bp |
Locus ID | 49 |
UniProt ID | P10323 |
Protein Families | Druggable Genome, Protease |
MW | 45.8 kDa |
Gene Summary | Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214256 | ACR (Myc-DDK-tagged)-Human acrosin (ACR) |
CNY 3,656.00 |
|
RC214256L1 | Lenti ORF clone of Human acrosin (ACR), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC214256L2 | Lenti ORF clone of Human acrosin (ACR), mGFP tagged |
CNY 5,890.00 |
|
RC214256L3 | Lenti ORF clone of Human acrosin (ACR), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC214256L4 | Lenti ORF clone of Human acrosin (ACR), mGFP tagged |
CNY 5,890.00 |
|
RG214256 | ACR (tGFP-tagged) - Human acrosin (ACR) |
CNY 4,370.00 |
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