PDE1B (NM_000924) Human Untagged Clone

CAT#: SC310288

PDE1B (untagged)-Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 1



  "NM_000924" in other vectors (6)

CNY 4,000.00

CNY 7,220.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
PDE1B mouse monoclonal antibody, clone OTI6E4 (formerly 6E4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HEL-S-79p; PDE1B1; PDES1B
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_000924 edited
CTTGGGCCGAGCCTAGAGACACCGGCCTGGCTGGTCCACGCCAGCCGCAGACCGTGGCTG
AGCATGGAGCTGTCCCCCCGCAGTCCTCCGGAGATGCTGGAGGAGTCGGATTGCCCGTCA
CCCCTGGAGCTGAAGTCAGCCCCCAGCAAGAAGATGTGGATTAAGCTTCGGTCTCTGCTG
CGCTACATGGTGAAGCAGTTGGAGAATGGGGAGATAAACATTGAGGAGCTGAAGAAAAAT
CTGGAGTACACAGCTTCTCTGCTGGAAGCCGTCTACATAGATGAGACACGGCAAATCTTG
GACACGGAGGACGAGCTGCAGGAGCTGCGGTCAGATGCCGTGCCTTCGGAGGTGCGGGAC
TGGCTGGCCTCCACCTTCACCCAGCAGGCCCGGGCCAAAGGCCGCCGAGCAGAGGAGAAG
CCCAAGTTCCGAAGCATTGTGCACGCTGTGCAGGCTGGGATCTTCGTGGAACGGATGTTC
CGGAGAACATACACCTCTGTGGGCCCCACTTACTCTACTGCGGTTCTCAACTGTCTCAAG
AACCTGGATCTCTGGTGCTTTGATGTCTTTTCCTTGAACCAGGCAGCAGATGACCATGCC
CTGAGGACCATTGTTTTTGAGTTGCTGACTCGGCATAACCTCATCAGCCGCTTCAAGATT
CCCACTGTGTTTTTGATGAGTTTCCTGGATGCCTTGGAGACAGGCTATGGGAAGTACAAG
AATCCTTACCACAACCAGATCCACGCAGCCGATGTTACCCAGACAGTCCATTGCTTCTTG
CTCCGCACAGGGATGGTGCACTGCCTGTCGGAGATTGAGCTCCTGGCCATCATCTTTGCT
GCAGCTATCCATGATTATGAGCACACGGGCACTACCAACAGCTTCCACATCCAGACCAAG
TCAGAATGTGCCATCGTGTACAATGATCGTTCAGTGCTGGAGAATCACCACATCAGCTCT
GTTTTCCGATTGATGCAGGATGATGAGATGAACATTTTCATCAACCTCACCAAGGATGAG
TTTGTAGAACTCCGAGCCCTGGTCATTGAGATGGTGTTGGCCACAGACATGTCCTGCCAT
TTCCAGCAAGTGAAGACCATGAAGACAGCCTTGCAACAGCTGGAGAGGATTGACAAGCCC
AAGGCCCTGTCTCTACTGCTCCATGCTGCTGACATCAGCCACCCAACCAAGCAGTGGTTG
GTCCACAGCCGTTGGACCAAGGCCCTCATGGAGGAATTCTTCCGTCAGGGTGACAAGGAG
GCAGAGTTGGGCCTGCCCTTTTCTCCACTCTGTGACCGCACTTCCACTCTAGTGGCACAG
TCTCAGATAGGGTTCATCGACTTCATTGTGGAGCCCACATTCTCTGTGCTGACTGACGTG
GCAGAGAAGAGTGTTCAGCCCCTGGCGGATGAGGACTCCAAGTCTAAAAACCAGCCCAGC
TTTCAGTGGCGCCAGCCCTCTCTGGATGTGGAAGTGGGAGACCCCAACCCTGATGTGGTC
AGCTTTCGTTCCACCTGGGTCAAGCGCATTCAGGAGAACAAGCAGAAATGGAAGGAACGG
GCAGCAAGTGGCATCACCAACCAGATGTCCATTGACGAGCTGTCCCCCTGTGAAGAAGAG
GCCCCCCCATCCCCTGCCGAAGATGAACACAACCAGAATGGGAATCTGGATTAGCCCTGG
GGCTGGCCCAGGTCTTCATTGAGTCCAAAGTGTTTGATGTCATCAGCACCATCCATCAGG
ACTGGCTCCCCCATCTGCTCCAAGGGAGCGTGGTCGTGGAAGAAACAACCCACCT
>OriGene 5' read for NM_000924 unedited
CGTCAAATTTTGTATACGACTCATATAGGGCGGCCGCGAATCAGATCTGGTACCGAGCTC
GGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTCTTGGGCCGAGCCTAGA
GACACCGGCCTGGCTGGTCCACGCCAGCCGCAGACCGTGGCTGAGCATGGAGCTGTCCCC
CCGCAGTCCTCCGGAGATGCTGGAGGAGTCGGATTGCCCGTCACCCCTGGAGCTGAAGTC
AGCCCCCAGCAAGAAGATGTGGATTAAGCTTCGGTCTCTGCTGCGCTACATGGTGAAGCA
GTTGGAGAATGGGGAGATAAACATTGAGGAGCTGAAGAAAAATCTGGAGTACACAGCTTC
TCTGCTGGAAGCCGTCTACATAGATGAGACACGGCAAATCTTGGACACGGAGGACGAGCT
GCAGGAGCTGCGGTCAGATGCCGTGCCTTCGGAGGTGCGGGACTGGCTGGCCTCCACCTT
CACCCAGCAGGCCCGGGCCAAAGGCCGCCGAGCAGAGGAGAAGCCCAAGTTCCGAAGCAT
TGTGCACGCTGTGCAGGCTGGGATCTTCGTGGAACGGATGTTCCGNGAGACATACACCTC
TGTGGGCCCCACTTACTCTACTGNCGTTCTCAACTGTCTCAAGAACCTGGATCTCTGGTG
CTTTGATGTCTTTTCCTTGAACCANGCAGCAGATGACCATGCCCTNGAGACCCATGTTTT
TGAGTTGCTGACTCGGCATAACCTCATCAGCCGCTTCAAGATTCCACTGGGTTTTTGATG
AAGTTCCCTGAATGCCTGGNAGACCGGGCTATGGGAAGTACAAGGAATCCTTACACAACC
AGATTCCACGCAGCCGATGTTACCCAGAAAGTCCACTGGTTCCTTGCTCCCCACAGGGAA
TGTGC
>Forward primer walk for NM_000924 unedited
TCCGTTACAAAAGGCACGGCCTTTCTACTGCCTCCTTGTCACCCTGACGNAAGATTCCTC
CATGAGGCCTTGGTCCAACGGCTGTGGACCAACCACTGCTTGGTTGGGTGGCTGATGTCA
GCAGCATGGAGCAGTAGAGACAGGGCCTTGGGCTTGTCAATCCTCTCCAGCTGTTGCAAG
GCTGTCTTCATGGTCTTCACTTGCTGGAAATGGCAGGACATGTCTGTGGCCAACACCATC
TCAATGACCAGGGCTCGGAGTTCTACAAACTCATCCTTGGTGAGGTTGATGAAAATGTTC
ATCTCATCATCCTGCATCAATCGGAAAACAGAGCTGATGTGGTGATTCTCCAGCACTGAA
CGATCATTGTACACGATGGCACATTCTGACTTGGTCTGGATGTGGAAGCTGTTGGTAGTG
CCCGTGTGCTCATAATCATGGATAGCTGCAGCAAAGATGATGGCCAGGAGCTCAATCTCC
GACAGGCAGTGCACCATCCCTGTGCGGAGCAAGAAGCAATGGACTGTCTGGGTAACATCG
GCTGCGTGGATCTGGTTGTGGTAAGGATTCTTGTACTTCCCATAGCCTGTCTCCAAGGCA
TCCAGGAAACTCATCAAAAACACAGTGGNGAATCTTGAAGCGGCTGATGAGGTTATGCCG
AGTCAGCAACTCANAAACAATGGTCCTCANGGCATGGTCATCTGCTGCCTGGTTCAAGGA
AAAGACATCAAAGCACCAGAGATCCAANGTTCTTGAGACAGTTGAGAACCGCAGTAGAGT
AAGTGGGGCCCCACAGAGGTGTATGTTCTCCGGGACATCCGTTN
Restriction Sites Please inquire     
ACCN NM_000924
Insert Size 1800 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_000924.2, NP_000915.1
RefSeq Size 3236 bp
RefSeq ORF 1611 bp
Locus ID 5153
UniProt ID Q01064
Domains PDEase, HDc
Protein Families Druggable Genome
Protein Pathways Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism
Gene Summary The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
Transcript Variant: This variant (1) encodes the longest isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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