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BTD (NM_000060) Human Untagged Clone
CAT＃: SC310282
BTD (untagged)-Human biotinidase (BTD)
CNY 4,048.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | biotinidase |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_000060 edited
ATGGCGCATGCGCATATTCAGGGCGGAAGGCGCGCTAAGAGCAGATTTGTGGTCTGCATT ATGTCTGGAGCCAGAAGTAAGCTTGCTCTTTTCCTCTGCGGCTGTTACGTGGTTGCCCTG GGAGCCCACACCGGGGAGGAGAGCGTGGCTGACCATCACGAGGCTGAATATTATGTGGCT GCCGTGTATGAGCATCCATCCATCCTGAGTCTGAACCCTCTGGCTCTCATCAGCCGCCAA GAGGCCTTGGAGCTCATGAACCAGAACCTTGACATCTATGAACAGCAAGTGATGACTGCA GCCCAAAAGGATGTACAGATTATAGTGTTTCCAGAAGATGGCATTCATGGATTCAACTTT ACAAGAACATCCATTTATCCATTTTTGGACTTCATGCCGTCTCCCCAGGTGGTCAGGTGG AACCCATGCCTGGAGCCTCACCGCTTCAATGACACAGAGGTGCTCCAGCGCCTGAGTTGT ATGGCCATCAGGGGAGATATGTTCTTGGTGGCCAATCTTGGGACAAAGGAGCCTTGTCAT AGCAGTGACCCAAGGTGCCCAAAAGATGGGAGATACCAGTTCAACACAAATGTCGTGTTC AGCAATAATGGAACCCTTGTTGACCGCTACCGTAAACACAACCTCTACTTTGAGGCAGCA TTCGATGTTCCTCTTAAAGTGGATCTCATCACCTTTGATACCCCCTTTGCTGGCAGGTTT GGCATCTTCACATGCTTTGATATATTGTTCTTTGACCCTGCCATCAGAGTCCTCAGAGAC TACAAGGTGAAGCATGTTGTGTACCCAACTGCCTGGATGAACCAGCTCCCACTCTTGGCA GCAATTGAGATTCAGAAAGCTTTTGCTGTTGCCTTTGGCATCAACGTTCTGGCAGCTAAT GTCCACCACCCAGTTCTGGGGATGACAGGAAGTGGCATACACACCCCTCTGGAGTCCTTT TGGTACCATGACATGGAAAATCCCAAAAGTCACCTTATAATTGCCCAGGTGGCCAAAAAT CCAGTGGGTCTCATTGGTGCAGAGAATGCAACAGGTGAAACGGACCCATCCCATAGTAAG TTTTTAAAAATTTTGTCAGGTGATCCGTACTGTGAGAAGGATGCTCAGGAAGTCCACTGT GATGAGGCCACCAAGTGGAACGTGAATGCTCCTCCCACATTTCACTCTGAGATGATGTAT GACAATTTCACCCTGGTCCCTGTCTGGGGAAAGGAAGGCTATCTCCACGTCTGTTCCAAT GGCCTCTGCTGTTATTTACTTTACGAGAGGCCCACCTTATCCAAAGAGCTGTATGCCCTG GGGGTCTTTGATGGGCTTCACACAGTACATGGCACTTACTACATCCAAGTGTGTGCCCTG GTCAGGTGTGGGGGTCTTGGCTTCGACACCTGCGGACAGGAAATCACAGAGGCCACGGGG ATATTTGAGTTTCACCTGTGGGGCAACTTCAGTACTTCCTATATCTTTCCTTTGTTTCTG ACCTCAGGGATGACCCTAGAAGTCCCTGACCAGCTTGGCTGGGAGAATGACCACTATTTC CTGAGGAAAAGTAGGCTGTCCTCTGGGCTGGTGACGGCGGCTCTCTATGGGCGCTTGTAT GAGAGGGACTAG >OriGene 5' read for NM_000060 unedited
CCCACAGGGACACAACTATACAGGGCGGCCGCGACCCGCACGACAAACAGGTAGAGCCGA CCCCGAGCCTCCGCCCCGCCCCCGAGACGGCCAGCCGGAGCGAATTCGGGGCTGTAAAGG GAGAATGGCGCATGCGCAAATTCAGGGCGGAAGGCGCGCTAAGAGCAGACCCGTGGCCCG CAACATGCCTGGAGCCAGAAGCAAGCCCGCTCCCCTCCTCCGCGGCTGTCACGCGGTCGC CCTGGGAGCCCACACCGGGGAGGAGAGCGTGGCTGACCATCACGAGGCTGAATATTATGT GGCTGCCGTGTATGAGCATCCATCCATCCTGAGTCTGAACCCTCTGGCTCTCATCAGCCG CCAAGAGGCCTTGGAGCTCATGAACCAGAACCTTGACATCTATGAACAGCAAGCGATGAC TGCAGCCCAAAAGGATGTACAGATTATAGTGTTTCCAGAAGATGGCATTCATGGATTCAA CCTTACAAGAACATCCATCTTATCCATTTTTGGACTTCATGCCGTCTCCCCAGGTGGTCA GGTGGAACCCATGCCTGGAGCCTCACCGCTTCAATGACACAGAGGTGCTCCAGCGCCTGA GTTGTATGGCCATCAGGGGAGATATGTTCTTGGTGGCCAATCTTGGGACAAAGGAGCCTT GTCATAGCAGTGACCCAAGGTGCCCAAAAGATGGGAGATACCAGTTCAACACAAATGTCG TGTTCAGCAATAATGGAACCCTTGTTGACCGCTACCGTAACACAACCTCTACTTTGAGCA GCATTCGATGTTCCTCTTAAAGTGGATCTCATCACCTTTGATACCCCCTTTGCTGGCAGT TTGGCATCTTCACATGCTTTGATAATATTGTTCT >Forward primer walk for NM_000060 unedited
CATCATACGTTCTGCGCTATGTCCCCACCCAGTTCTGGGGATGACAGGAAGTGGCATACA CACCCCTCTGGAGTCCTTTTGGTACCATGACATGGAAAATCCCAAAAGTCACCTTATAAT TGCCCAGGTGGCCAAAAATCCAGTGGGTCTCATTGGTGCAGAGAATGCAACAGGTGAAAC GGACCCATCCCATAGTAAGTTTTTAAAAATTTTGTCAGGTGATCCGTACTGTGAGAAGGA TGCTCAGGAAGTCCACTGTGATGAGGCCACCAAGTGGAACGTGAATGCTCCTCCCACATT TCACTCTGAGATGATGTATGACAATTTCACCCTGGTCCCTGTCTGGGGAAAGGAAGGCTA TCTCCACGTCTGTTCCAATGGCCTCTGCTGTTATTTACTTTACGAGAGGCCCACCTTATC CAAAGAGCTGTATGCCCTGGGGGTCTTTGATGGGCTTCACACAGTACATGGCACTTACTA CATCCAAGTGTGTGCCCTGGTCAGGTGTGGGGGTCTTGGCTTCGACACCTGCGGACAGGA AATCACAGAGGCCACGGGGATATTTGAGTTTCACCTGTGGGGCAACTTCAGTACTTCCTA TATCTTTCCTTTGTTTCTGACCTCAGGGATGACCCTAGAAGTCCCTGACCAGCTTGGCTG GGAGAATGACCACTATTTTCCTGAGGAAAAGTAGGCTGTCCTCTGGGCTGGTGACGGCGG CTCTCTATGGGCGCTTGTATGAGAGGGACTAGGAAAAGTGTGTGGTCTGTGGGGCGGACT CTGGCCATCATGTTGACAGCCTTGCACTTCCACAGGCTACAAGCCCTGGGACCATCTTTC TGCCTTAAGGCAGGAGCCCCAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000060 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000060.2, NP_000051.1 |
| RefSeq Size | 2082 bp |
| RefSeq ORF | 1632 bp |
| Locus ID | 686 |
| UniProt ID | P43251 |
| Domains | CN_hydrolase |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Biotin metabolism, Metabolic pathways |
| Gene Summary | The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region and uses a downstream start codon compared to variant 1. The resulting protein (isoform 3) is shorter and has a distinct N-terminus compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204153 | BTD (Myc-DDK-tagged)-Human biotinidase (BTD) |
CNY 4,040.00 |
|
| RC204153L3 | Lenti ORF clone of Human biotinidase (BTD), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204153L4 | Lenti ORF clone of Human biotinidase (BTD), mGFP tagged |
CNY 5,890.00 |
|
| RG204153 | BTD (tGFP-tagged) - Human biotinidase (BTD) |
CNY 5,640.00 |
