BTN3A3 (NM_006994) Human Untagged Clone

CAT#: SC310243

BTN3A3 (untagged)-Human butyrophilin, subfamily 3, member A3 (BTN3A3), transcript variant 1



  "NM_006994" in other vectors (6)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms BTF3; BTN3.3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC310243 representing NM_006994.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAAAATGGCAAGTTCCCTGGCTTTCCTTCTGCTCAACTTTCATGTCTCCCTCTTCTTGGTCCAGCTG
CTCACTCCTTGCTCAGCTCAGTTTTCTGTGCTTGGACCCTCTGGGCCCATCCTGGCCATGGTGGGTGAA
GACGCTGATCTGCCCTGTCACCTGTTCCCGACCATGAGTGCAGAGACCATGGAGCTGAGGTGGGTGAGT
TCCAGCCTAAGGCAGGTGGTGAACGTGTATGCAGATGGAAAGGAAGTGGAAGACAGGCAGAGTGCACCG
TATCGAGGGAGAACTTCGATTCTGCGGGATGGCATCACTGCAGGGAAGGCTGCTCTCCGAATACACAAC
GTCACAGCCTCTGACAGTGGAAAGTACTTGTGTTATTTCCAAGATGGTGACTTCTACGAAAAAGCCCTG
GTGGAGCTGAAGGTTGCAGCATTGGGTTCTGATCTTCACATTGAAGTGAAGGGTTATGAGGATGGAGGG
ATCCATCTGGAGTGCAGGTCCACTGGCTGGTACCCCCAACCCCAAATAAAGTGGAGCGACACCAAGGGA
GAGAACATCCCGGCTGTGGAAGCACCTGTGGTTGCAGATGGAGTGGGCCTGTATGCAGTAGCAGCATCT
GTGATCATGAGAGGCAGCTCTGGTGGGGGTGTATCCTGCATCATCAGAAATTCCCTCCTCGGCCTGGAA
AAGACAGCCAGCATATCCATCGCAGACCCCTTCTTCAGGAGCGCCCAGCCCTGGATCGCGGCCCTGGCA
GGGACCCTGCCTATCTCGTTGCTGCTTCTCGCAGGAGCCAGTTACTTCTTGTGGAGACAACAGAAGGAA
AAAATTGCTCTGTCCAGGGAGACAGAAAGAGAGCGAGAGATGAAAGAAATGGGATACGCTGCAACAGAG
CAAGAAATAAGCCTAAGAGAGAAGCTCCAGGAGGAACTCAAGTGGAGGAAAATCCAGTACATGGCTCGT
GGAGAGAAGTCTTTGGCCTATCATGAATGGAAAATGGCCCTCTTCAAACCTGCGGATGTGATTCTGGAT
CCAGACACGGCAAACGCCATCCTCCTTGTTTCTGAGGACCAGAGGAGTGTGCAGCGTGCTGAAGAGCCG
CGGGATCTGCCAGACAACCCTGAGAGATTTGAATGGCGTTACTGTGTCCTTGGCTGTGAAAACTTCACA
TCAGGGAGACATTACTGGGAGGTGGAAGTGGGGGACAGAAAAGAGTGGCATATTGGGGTATGTAGTAAG
AACGTGGAGAGGAAAAAAGGTTGGGTCAAAATGACACCGGAGAACGGATACTGGACTATGGGCCTGACT
GATGGGAATAAGTATCGGGCTCTCACTGAGCCCAGAACCAACCTGAAACTTCCTGAGCCTCCTAGGAAA
GTGGGGATCTTCCTGGACTATGAGACTGGAGAGATCTCGTTCTATAATGCCACAGATGGATCTCATATC
TACACCTTTCCGCACGCCTCTTTCTCTGAGCCTCTATATCCTGTTTTCAGAATTTTGACCTTGGAGCCC
ACTGCCCTGACCATTTGCCCAATACCAAAAGAAGTAGAGAGTTCCCCCGATCCTGACCTAGTGCCTGAT
CATTCCCTGGAGACACCACTGACCCCGGGCTTAGCTAATGAAAGTGGGGAGCCTCAGGCTGAAGTAACA
TCTCTGCTTCTCCCTGCCCACCCTGGAGCTGAGGTCTCCCCTTCTGCAACAACCAATCAGAACCATAAG
CTACAGGCACGCACTGAAGCACTTTACTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_006994
Insert Size 1755 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006994.4
RefSeq Size 3019 bp
RefSeq ORF 1755 bp
Locus ID 10384
UniProt ID O00478
Domains IGv, IG, SPRY, PRY
Protein Families Druggable Genome, Transmembrane
MW 65 kDa
Gene Summary The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1; MIM 613590) and BTN3 (e.g., BNT3A3) genes, which have undergone tandem duplication, resulting in 3 copies of each (summary by Smith et al., 2010 [PubMed 20208008]).[supplied by OMIM, Nov 2010]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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