PDE4D (NM_006203) Human Untagged Clone

CAT#: SC310179

PDE4D (untagged)-Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 2



  "NM_006203" in other vectors (6)

CNY 4,936.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-PDE4D Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ACRDYS2; DPDE3; HSPDE4D; PDE4DN2; PDE43; STRK1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_006203 edited
ATGCACGTGAATAATTTTCCCTTTAGAAGGCATTCCTGGATATGTTTTGATGTGGACAAT
GGCACATCTGCGGGACGGAGTCCCTTGGATCCCATGACCAGCCCAGGATCCGGGCTAATT
CTCCAAGCAAATTTTGTCCACAGTCAACGACGGGAGTCCTTCCTGTATCGATCCGACAGC
GATTATGACCTCTCTCCAAAGTCTATGTCCCGGAACTCCTCCATTGCCAGTGATATACAC
GGAGATGACTTGATTGTGACTCCATTTGCTCAGGTCTTGGCCAGTCTGCGAACTGTACGA
AACAACTTTGCTGCATTAACTAATTTGCAAGATCGAGCACCTAGCAAAAGATCACCCATG
TGCAACCAACCATCCATCAACAAAGCCACCATAACAGAGGAGGCCTACCAGAAACTGGCC
AGCGAGACCCTGGAGGAGCTGGACTGGTGTCTGGACCAGCTAGAGACCCTACAGACCAGG
CACTCCGTCAGTGAGATGGCCTCCAACAAGTTTAAAAGGATGCTTAATCGGGAGCTCACC
CATCTCTCTGAAATGAGTCGGTCTGGAAATCAAGTGTCAGAGTTTATATCAAACACATTC
TTAGATAAGCAACATGAAGTGGAAATTCCTTCTCCAACTCAGAAGGAAAAGGAGAAAAAG
AAAAGACCAATGTCTCAGATCAGTGGAGTCAAGAAATTGATGCACAGCTCTAGTCTGACT
AATTCAAGTATCCCAAGGTTTGGAGTTAAAACTGAACAAGAAGATGTCCTTGCCAAGGAA
CTAGAAGATGTGAACAAATGGGGTCTTCATGTTTTCAGAATAGCAGAGTTGTCTGGTAAC
CGGCCCTTGACTGTTATCATGCACACCATTTTTCAGGAACGGGATTTATTAAAAACATTT
AAAATTCCAGTAGATACTTTAATTACATATCTTATGACTCTCGAAGACCATTACCATGCT
GATGTGGCCTATCACAACAATATCCATGCTGCAGATGTTGTCCAGTCTACTCATGTGCTA
TTATCTACACCTGCTTTGGAGGCTGTGTTTACAGATTTGGAGATTCTTGCAGCAATTTTT
GCCAGTGCAATACATGATGTAGATCATCCTGGTGTGTCCAATCAATTTCTGATCAATACA
AACTCTGAACTTGCCTTGATGTACAATGATTCCTCAGTCTTAGAGAACCATCATTTGGCT
GTGGGCTTTAAATTGCTTCAGGAAGAAAACTGTGACATTTTCCAGAATTTGACCAAAAAA
CAAAGACAATCTTTAAGGAAAATGGTCATTGACATCGTACTTGCAACAGATATGTCAAAA
CACATGAATCTACTGGCTGATTTGAAGACTATGGTTGAAACTAAGAAAGTGACAAGCTCT
GGAGTTCTTCTTCTTGATAATTATTCCGATAGGATTCAGGTTCTTCAGAATATGGTGCAC
TGTGCAGATCTGAGCAACCCAACAAAGCCTCTCCAGCTGTACCGCCAGTGGACGGACCGG
ATAATGGAGGAGTTCTTCCGCCAAGGAGACCGAGAGAGGGAACGTGGCATGGAGATAAGC
CCCATGTGTGACAAGCACAATGCTTCCGTGGAAAAATCACAGGTGGGCTTCATAGACTAT
ATTGTTCATCCCCTCTGGGAGACATGGGCAGACCTCGTCCACCCTGACGCCCAGGATATT
TTGGACACTTTGGAGGACAATCGTGAATGGTACCAGAGCACAATCCCTCAGAGCCCCTCT
CCTGCACCTGATGACCCAGAGGAGGGCCGGCAGGGTCAAACTGAGAAATTCCAGTTTGAA
CTAACTTTAGAGGAAGATGGTGAGTCAGACACGGAAAAGGACAGTGGCAGTCAAGTGGAA
GAAGACACTAGCTGCAGTGACTCCAAGACTCTTTGTACTCAAGACTCAGAGTCTACTGAA
ATTCCCCTTGATGAACAGGTTGAAGAGGAGGCAGTAGGGGAAGAAGAGGAAAGCCAGCCT
GAAGCCTGTGTCATAGATGATCGTTCTCCTGACACGTAA
>Reverse primer walk for NM_006203 unedited
CTACATGTAAGGACTCCCGTCTGTGACTGTGGACAAAATTTGCTTGGACAATTAACCCGG
ATCCTGGGCTGCTCATGGGATCCAAGGGACTCCGTCCCGCAAATGTGCCATTGTCCACAT
CAAAACATATCCAGGAATGCCTTCTAGAGGGAAAATTATTCACGTGCATCATGTTCCCAT
ATCTTCTGTCATTAATATTTTTCTTGTCTCCTACGTTACATGTAGTGATTTTTACAGATG
AATTCCAACTCTCTTGTTTGAGCAACTATCGTGCGCAATTATTGCAACACGTATTGAATC
CCAAAAAAAAAAAAAAAATCTCCCCCCACCAATAAAAACAAATAGCACTTACAAAAGGCC
TTCCATGATTTGAAAAAACAAACATCACAGAACACTGCAGGTATGGATCCATCCATTTAG
GTTCCACTATTTAGTCTACATACTCAAATAACCAGTTCAACTAATAATCCTAACTGGCGG
CCAATATTGTCCTGGAAAAATCTAACCGCCTTGAGTGTGAATTAATGCATCCTTAACGAA
GGACTCTGGAATGTGGGAAGAGAGGAGTCAAAAGACAGTTTCTTGATATCCTTGCTAAAC
AAGTGTTCGCATGGAAATGGAAAACTTCAGGTTGCTTTGCCATGCTCGTCTTTAGCAATG
AATATTGCTTTCTTCTCTTTCTTAGCACATATTCATGTCTGGTCACTTTAACGCAGTGCT
ACCGTCTGAGACGTGTCGTACTAAGCCTGTGCTGACGGGCTAGCAACCTGTATCACCAAA
GCCCACTTCTTTCCCAGATTCAGAATTGCTGGTTCAACTGCTAAGTANG
>OriGene 3' genomic read for NM_006203 unedited
GTTCATACTGTTGCATATTGCCCACNAAGCTGCTCAAACAAAGATTGGAATTCATCTGTA
AAAATCACTACATGTAACGTAGGAGACAAGAAAAATATTAATGACAGAAGATCTGCGAAC
ATGATGCACGTGAATAATTTTCCCTTTAGAAGGCATTCCTGGATATGTTTTGATGTGGAC
AATGGCACATCTGCGGGACGGAGTCCCTTGGATCCCATGACCAGCCCCAGATCCGGNNCT
ATTCTNCNAGCANATTTTGTCCACAGTCAACGACGGNAGTCCCTTCTGTATCGATCCGAC
AGCGATTATGACCTCTCTCCAAAGTCTATGTCCCNGGACTCCTNCATTTGCAGTGATATA
CCCGAGAATGACTTGAGTGGGACTCCATTTGCCTCAGGTCTGGGCCATCTTGCAAACTGT
ACAAAACAACTTTTGCTGCTTAACCTAATTTGCAGATCGGCCCCCTGGCCAAGATCACCC
TTGTGCCAACCACCCTCCTTTAAACAAAGCCCCCTTAACAAAGAGGCCCTCCCCAAAACG
GGCCCCCCGAACCCCTGAAAAACCTGACTTGGGTTTTGGCCCCCTTTTGAAACCCTTAAA
ACAGACCCCTCCTCCGTGGAAAAGGGCCCCCCCCAATTTTTTAAAAGAGGGGTTTTATTG
GGGGGCCCCCCCCCCCCCTTCTTTAAAAAAAAGGGGGGCCCGGGGGAAAAAAAAGGGGGC
GGAGATTTTTATTATTACAACCACTTTTTTTTAATAATAACACCCCGAGAGGGGGGGAGA
AACTCTTTTCTCTCCCCCCCCCCCGGGGGGGGGGGGGGAGAAAAAAAAAAACACCCCTCT
TTCTCTCTTCTAATTGGGGGTTGGACAAAAAAATTTTTTCGCCCCCCCCTCTTTTTTTT
Restriction Sites Please inquire     
ACCN NM_006203
Insert Size 7000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006203.3, NP_006194.2
RefSeq Size 5876 bp
RefSeq ORF 2022 bp
Locus ID 5144
UniProt ID Q08499
Domains PDEase
Protein Families Druggable Genome
Protein Pathways Progesterone-mediated oocyte maturation, Purine metabolism
Gene Summary This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009]
Transcript Variant: This variant (2) differs in the 5' UTR and coding region, compared to variant 1. Isoform PDE4D3 (also known as isoform 2) is shorter and has a distinct N-terminus, compared to isoform PDE4D4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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