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RNF43 (NM_017763) Human Untagged Clone
CAT＃: SC310117
RNF43 (untagged)-Human ring finger protein 43 (RNF43)
CNY 8,616.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RNF124; SSPCS; URCC |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_017763 edited
ATGAGTGGTGGCCACCAGCTGCAGCTGGCTGCCCTCTGGCCCTGGCTGCTGATGGCTACC CTGCAGGCAGGCTTTGGACGCACAGGACTGGTACTGGCAGCAGCGGTGGAGTCTGAAAGA TCAGCAGAACAGAAAGCTATTATCAGAGTGATCCCCTTGAAAATGGACCCCACAGGAAAA CTGAATCTCACTTTGGAAGGTGTGTTTGCTGGTGTTGCTGAAATAACTCCAGCAGAAGGA AAATTAATGCAGTCCCACCCGCTGTACCTGTGCAATGCCAGTGATGACGACAATCTGGAG CCTGGATTCATCAGCATCGTCAAGCTGGAGAGTCCTCGACGGGCCCCCCGCCCCTGCCTG TCACTGGCTAGCAAGGCTCGGATGGCGGGTGAGCGAGGAGCCAGTGCTGTCCTCTTTGAC ATCACTGAGGATCGAGCTGCTGCTGAGCAGCTGCAGCAGCCGCTGGGGCTGACCTGGCCA GTGGTGTTGATCTGGGGTAATGACGCTGAGAAGCTGATGGAGTTTGTGTACAAGAACCAA AAGGCCCATGTGAGGATTGAGCTGAAGGAGCCCCCGGCCTGGCCAGATTATGATGTGTGG ATCCTAATGACAGTGGTGGGCACCATCTTTGTGATCATCCTGGCTTCGGTGCTGCGCATC CGGTGCCGCCCCCGCCACAGCAGGCCGGATCCGCTTCAGCAGAGAACAGCCTGGGCCATC AGCCAGCTGGCCACCAGGAGGTACCAGGCCAGCTGCAGGCAGGCCCGGGGTGAGTGGCCA GACTCAGGGAGCAGCTGCAGCTCAGCCCCTGTGTGTGCCATCTGTCTGGAGGAGTTCTCT GAGGGGCAGGAGCTACGGGTCATTTCCTGCCTCCATGAGTTCCATCGTAACTGTGTGGAC CCCTGGTTACATCAGCATCGGACTTGCCCCCTCTGCATGTTCAACATCACAGAGGGAGAT TCATTTTCCCAGTCCCTGGGACCCTCTCGATCTTACCAAGAACCAGGTCGAAGACTCCAC CTCATTCGCCAGCATCCCGGCCATGCCCACTACCACCTCCCTGCTGCCTACCTGTTGGGC CCTTCCCGGAGTGCAGTGGCTCGGCCCCCACGACCTGGTCCCTTCCTGCCATCCCAGGAG CCAGGCATGGGCCCTCGGCATCACCGCTTCCCCAGAGCTGCACATCCCCGGGCTCCAGGA GAGCAGCAGCGCCTGGCAGGAGCCCAGCACCCCTATGCACAAGGCTGGGGACTGAGCCAC CTCCAATCCACCTCACAGCACCCTGCTGCTTGCCCAGTGCCCCTACGCCGGGCCAGGCCC CCTGACAGCAGTGGATCTGGAGAAAGCTATTGCACAGAACGCAGTGGGTACCTGGCAGAT GGGCCAGCCAGTGACTCCAGCTCAGGGCCCTGTCATGGCTCTTCCAGTGACTCTGTGGTC AACTGCACGGACATCAGCCTACAGGGGGTCCATGGCAGCAGTTCTACTTTCTGCAGCTCC CTAAGCAGTGACTTTGACCCCCTAGTGTACTGCAGCCCTAAAGGGGATCCCCAGCGAGTG GACATGCAGCCTAGTGTGACCTCTCGGCCTCGTTCCTTGGACTCGGTGGTGCCCACAGGG GAAACCCAGGTTTCCAGCCATGTCCACTACCACCGCCACCGGCACCACCACTACAAAAAG CGGTTCCAGTGGCATGGCAGGAAGCCTGGCCCAGAAACCGGAGTCCCCCAGTCCAGGCCT CCTATTCCTCGGACACAGCCCCAGCCAGAGCCACCTTCTCCTGATCAGCAAGTCACCAGA TCCAACTCAGCAGCCCCTTCGGGGCGGCTCTCTAACCCACAGTGCCCCAGGGCCCTCCCT GAGCCAGCCCCTGGCCCAGTTGACGCCTCCAGCATCTGCCCCAGTACCAGCAGTCTGTTC AACTTGCAAAAATCCAGCCTCTCTGCCCGACACCCACAGAGGAAAAGGCGGGGGGGTCCC TCCGAGCCCACCCCTGGCTCTCGGCCCCAGGATGCAACTGTGCACCCAGCTTGCCAGATT TTTCCCCATTACACCCCCAGTGTGGCATATCCTTGGTCCCCAGAGGCACACCCCTTGATC TGTGGACCTCCAGGCCTGGACAAGAGGCTGCTACCAGAAACCCCAGGCCCCTGTTACTCA AATTCACAGCCAGTGTGGTTGTGCCTGACTCCTCGCCAGCCCCTGGAACCACATCCACCT GGGGAGGGGCCTTCTGAATGGAGTTCTGACACCGCAGAGGGCAGGCCATGCCCTTATCCG CACTGCCAGGTGCTGTCGGCCCAGCCTGGCTCAGAGGAGGAACTCGAGGAGCTGTGTGAA CAGGCTGTGTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_017763 |
| Insert Size | 2400 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017763.3, NP_060233.3 |
| RefSeq Size | 5585 bp |
| RefSeq ORF | 2352 bp |
| Locus ID | 54894 |
| UniProt ID | Q68DV7 |
| Domains | RING |
| Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
| Gene Summary | The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Both variants 1 and 2 encode the same protein. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214013 | RNF43 (Myc-DDK-tagged)-Human ring finger protein 43 (RNF43) |
CNY 8,600.00 |
|
| RC214013L1 | Lenti ORF clone of Human ring finger protein 43 (RNF43), Myc-DDK-tagged |
CNY 11,000.00 |
|
| RC214013L2 | Lenti ORF clone of Human ring finger protein 43 (RNF43), mGFP tagged |
CNY 11,000.00 |
|
| RC214013L3 | Lenti ORF clone of Human ring finger protein 43 (RNF43), Myc-DDK-tagged |
CNY 11,000.00 |
|
| RC214013L4 | Lenti ORF clone of Human ring finger protein 43 (RNF43), mGFP tagged |
CNY 11,000.00 |
|
| RG214013 | RNF43 (tGFP-tagged) - Human ring finger protein 43 (RNF43) |
CNY 4,370.00 |
