VAV2 (NM_003371) Human Untagged Clone

CAT#: SC310084

VAV2 (untagged)-Human vav 2 guanine nucleotide exchange factor (VAV2), transcript variant 2



  "NM_003371" in other vectors (6)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-VAV2 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms VAV-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC310084 representing NM_003371.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGAGCAGTGGCGGCAGTGCGGCCGCTGGCTCATCGATTGCAAGGTCCTGCCGCCCAACCACCGGGTG
GTGTGGCCCTCGGCCGTGGTCTTCGACCTGGCGCAGGCGCTGCGCGACGGGGTCCTTCTGTGCCAGCTG
CTGCACAACCTCTCCCCCGGCTCCATCGACCTCAAGGACATCAACTTCCGGCCGCAGATGTCCCAGTTT
CTGTGTTTGAAGAACATACGCACCTTCCTGAAAGTCTGCCACGATAAATTTGGATTAAGGAACAGCGAG
CTGTTTGACCCCTTTGACCTCTTCGATGTGCGAGACTTTGGAAAGGTCATCTCCGCGGTGTCGAGGCTC
TCCCTGCACAGCATCGCGCAGAACAAAGGGATCAGGCCTTTTCCCTCAGAGGAGACCACAGAGAATGAC
GATGACGTCTACCGCAGCCTGGAGGAGCTGGCCGACGAGCATGACCTGGGGGAGGACATCTACGACTGC
GTCCCGTGTGAGGATGGAGGGGACGACATCTACGAGGACATCATCAAGGTGGAGGTGCAGCAGCCCATG
AAAATGGGCATGACTGAAGATGACAAGAGGAACTGCTGCCTGCTGGAGATCCAGGAGACCGAGGCCAAG
TACTACCGCACCCTGGAGGACATTGAGAAGAACTACATGAGCCCCCTGCGGCTGGTGCTGAGCCCGGCG
GACATGGCAGCTGTCTTCATTAACCTGGAGGACCTGATCAAGGTGCATCACAGCTTCCTGAGGGCCATC
GACGTGTCCGTGATGGTGGGGGGCAGCACGCTGGCCAAGGTCTTCCTCGATTTCAAGGAAAGGCTTCTG
ATCTACGGGGAGTACTGCAGCCACATGGAGCACGCCCAGAACACACTGAACCAGCTCCTGGCCAGCCGG
GAGGACTTCAGGCAGAAAGTCGAGGAGTGCACACTGAAGGTCCAGGATGGAAAATTTAAGCTGCAAGAC
CTGCTGGTGGTCCCCATGCAGAGGGTGCTCAAATACCACCTGCTCTTGAAGGAGCTTCTGAGCCATTCT
GCGGAACGGCCTGAGAGGCAGCAGCTCAAAGAAGCACTGGAAGCCATGCAGGACTTGGCGATGTACATC
AATGAAGTTAAACGGGACAAGGAGACCTTGAGGAAAATCAGCGAATTTCAGAGTTCTATAGAAAATTTG
CAAGTGAAACTGGAGGAATTTGGAAGACCAAAGATTGACGGGGAACTGAAAGTCCGGTCCATAGTCAAC
CACACCAAGCAGGACAGGTACTTGTTCCTGTTTGACAAGGTGGTCATCGTCTGCAAGCGGAAGGGCTAC
AGCTACGAGCTCAAGGAGATCATCGAGCTGCTGTTCCACAAGATGACCGACGACCCCATGAACAACAAG
GACGTCAAGAAGTGGTCCTACGGCTTCTACCTAATTCACCTTCAAGGAAAGCAGGGCTTCCAGTTTTTC
TGCAAAACAGAAGATATGAAGAGGAAGTGGATGGAGCAGTTTGAGATGGCCATGTCAAACATCAAGCCA
GACAAAGCCAATGCCAACCACCACAGTTTCCAGATGTACACGTTTGACAAGACCACCAACTGCAAAGCC
TGCAAAATGTTCCTCAGGGGCACCTTCTACCAGGGATACATGTGTACCAAGTGTGGCGTCGGGGCACAC
AAGGAGTGCCTGGAAGTGATACCTCCCTGCAAGTTCACTTCTCCTGCAGATCTGGACGCCTCCGGAGCG
GGACCAGGTCCCAAGATGGTGGCCATGCAGAATTACCATGGCAACCCAGCCCCTCCCGGGAAGCCTGTG
CTGACCTTCCAGACGGGCGACGTGCTTGAGCTGCTGAGGGGCGACCCTGAGTCTCCGTGGTGGGAGGGT
CGTCTGGTACAAACCAGGAAGTCAGGGTATTTCCCCAGCTCATCTGTGAAGCCCTGCCCTGTGGATGGA
AGGCCGCCCATCAGCCGGCCGCCATCCCGGGAGATCGACTACACTGCATACCCCTGGTTTGCAGGTAAC
ATGGAGAGGCAGCAGACGGACAACCTGCTCAAGTCCCACGCCAGCGGGACCTACCTGATCAGGGAGCGG
CCTGCCGAGGCTGAGCGCTTTGCAATAAGCATCAAGTTCAATGATGAGGTGAAGCACATCAAGGTGGTG
GAGAAGGACAACTGGATCCACATCACAGAGGCCAAGAAATTCGACAGCCTCCTGGAGTTGGTGGAGTAC
TACCAGTGCCACTCACTGAAGGAGAGCTTCAAGCAGCTGGACACCACACTCAAGTACCCCTACAAGTCC
CGGGAACGTTCGGCCTCCAGGGCCTCCAGCCGGTCCCCAGTGTTCACGCCCCGCGTCATCGGCACAGCT
GTGGCCAGGTATAACTTTGCCGCCCGAGATATGAGGGAGCTTTCGCTGCGGGAGGGTGACGTGGTGAGG
ATCTACAGCCGCATCGGCGGAGACCAGGGCTGGTGGAAGGGCGAGACCAACGGACGGATTGGCTGGTTT
CCTTCAACGTACGTAGAAGAGGAGGGCATCCAGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_003371
Insert Size 2520 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003371.3
RefSeq Size 4748 bp
RefSeq ORF 2520 bp
Locus ID 7410
UniProt ID P52735
Domains RhoGEF, SH2, SH3, CH, PH, DAG_PE-bind
Protein Families Druggable Genome
Protein Pathways B cell receptor signaling pathway, Chemokine signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, T cell receptor signaling pathway
MW 97 kDa
Gene Summary VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
Transcript Variant: This variant (2) lacks three single exons in the CDS, as compared to variant 1. The reading frame is not changed and the resulting isoform (2) lacks three internal segments, as compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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