Myosin Phosphatase 2 (PPP1R12B) (NM_002481) Human Untagged Clone

CAT#: SC310021

PPP1R12B (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 12B (PPP1R12B), transcript variant 1



  "NM_002481" in other vectors (6)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


PPP1R12B Antibody - Middle region
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms MYPT2; PP1bp55
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC310021 representing NM_002481.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCGGAACTGGAGCACCTAGGAGGGAAGCGGGCAGAGTCGGCGCGAATGCGGCGGGCAGAGCAGCTT
CGGCGCTGGCGGGGCTCGCTGACAGAGCAGGAGCCTGCGGAGCGACGAGGCGCGGGGCGGCAGCCGCTG
ACCAGGCGCGGGAGCCCCAGGGTCCGCTTCGAGGACGGTGCTGTCTTTCTGGCCGCCTGCTCTAGCGGG
GACACCGACGAGGTGAGAAAGCTTCTGGCAAGAGGTGCTGATATCAACACGGTCAACGTGGACGGCTTG
ACAGCCCTGCACCAGGCATGTATTGATGAAAATTTGGACATGGTGAAGTTTCTGGTGGAGAACAGAGCC
AATGTAAACCAGCAAGACAACGAGGGCTGGACACCCCTTCATGCAGCAGCTTCCTGTGGCTATCTCAAC
ATAGCAGAGTATTTCATTAATCACGGAGCCAGTGTAGGTATTGTCAATAGTGAAGGTGAAGTTCCCTCT
GACCTTGCAGAAGAGCCAGCCATGAAGGATCTTCTTCTGGAGCAAGTAAAGAAGCAAGGAGTTGATCTA
GAGCAGTCAAGAAAAGAAGAAGAGCAGCAGATGTTGCAGGATGCCCGCCAGTGGCTCAACAGTGGGAAA
ATAGAGGATGTGAGGCAGGCTCGCTCAGGGGCTACAGCCCTTCATGTGGCTGCTGCCAAGGGCTACTCT
GAAGTCCTCAGACTTTTAATTCAGGCTGGCTATGAACTCAATGTTCAGGATTATGATGGCTGGACTCCC
CTCCATGCTGCTGCACACTGGGGAGTGAAGGAGGCTTGCTCCATCCTGGCAGAAGCACTTTGTGACATG
GATATTCGAAATAAACTGGGCCAGACACCATTTGATGTGGCTGATGAGGGTCTCGTGGAGCATTTGGAG
TTGCTCCAGAAGAAGCAGAATGTGCTTCGAAGTGAAAAGGAGACACGGAATAAACTCATTGAGTCAGAT
CTGAACAGCAAGATTCAGAGTGGGTTCTTTAAGAACAAAGAGAAGATGCTCTATGAGGAGGAGACACCT
AAGTCCCAAGAAATGGAGGAAGAAAATAAAGAATCTAGTAGCTCCAGCTCAGAGGAGGAGGAAGGTGAA
GATGAAGCTTCTGAGTCAGAAACTGAGAAGGAGGCAGATAAAAAGCCAGAAGCCTTTGTCAATCATTCC
AACTCTGAAAGCAAGAGTAGTATCACAGAGCAGATACCAGCACCAGCTCAAAATACCTTCTCTGCCTCT
TCTGCTAGGAGGTTCTCTTCTGGCCTTTTTAACAAGCCAGAAGAGCCCAAAGATGAATCTCCTTCTTCA
TGGAGATTGGGACTGAGAAAAACTGGCAGCCACAACATGCTGAGTGAGGTGGCCAATTCCAGGGAACCT
ATAAGGGACCGAGGCTCTTCCATCTATCGCTCCTCTTCAAGCCCTCGGATTTCTGCTCTACTGGACAAC
AAAGATAAGGAGAGAGAAAACAAAAGCTATATTAGTTCACTAGCACCCCGGAAGCTCAACAGCACAAGT
GATATTGAAGAAAAGGAGAACAGAGAATCAGCTGTTAATCTAGTGAGGAGTGGCTCCTATACCCGGCAG
CTATGGAGGGATGAAGCAAAAGGAAATGAAATCCCACAGACAATTGCTCCCTCCACCTATGTATCAACT
TACTTGAAAAGGACTCCTCACAAATCCCAGGCCGACACAACAGCAGAGAAAACAGCAGACAATGTCTCT
TCTAGCACCCCGCTCTGTGTGATCACCAATCGCCCTCTTCCTAGCACTGCCAATGGGGTTACAGCTACT
CCTGTGCTCTCCATTACTGGAACAGATTCCTCTGTGGAAGCCAGGGAGAAGAGGAGGTCCTATCTGACT
CCTGTACGGGATGAGGAAGCAGAGTCTTTACGGAAAGCACGCTCCAGACAAGCTCGGCAGACACGAAGG
TCTACTCAAGGTGTCACCCTAACAGACCTTCAAGAAGCAGAAAGGACATTCAGCCGGTCGAGGGCAGAG
AGGCAAGCTCAGGAGCAGCCTCGTGAGAAGCCCACAGACACTGAAGGGCTTGAGGGGAGCCCTGAGAAG
CATGAGCCCTCAGCAGTTCCAGCAACAGAAGCTGGGGAGGGCCAGCAGCCCTGGGGCAGGAGTCTGGAT
GAAGAGCCTATCTGTCATCGCCTGAGGTGCCCAGCTCAGCCAGACAAACCCACCACGCCAGCATCTCCT
TCTACGTCAAGACCCTCACTCTACACCAGTTCCCACCTGCTATGGACAAATAGATTTTCAGTCCCTGAT
TCTGAGAGTTCAGAGACTACCACAAACACTACAACTGCAAAGGAAATGGACAAAAATGAGAATGAAGAA
GCAGATTTGGATGAGCAGTCCTCTAAGAGGCTGTCCATCCGAGAGAGGAGGCGGCCCAAGGAACGACGA
AGAGGCACAGGCATCAATTTCTGGACAAAGGATGAGGATGAAACTGATGGCTCTGAAGAGGTCAAAGAA
ACGTGGCATGAAAGACTTTCTAGGTTGGAATCGGGAGGTAGTAATCCTACAACCAGTGATTCTTACGGT
GACCGGGCTTCAGCAAGAGCCCGTCGGGAGGCCCGGGAGGCCCGCCTAGCCACCCTGACCAGCCGTGTA
GAAGAAGACAGCAACAGAGATTATAAAAAACTCTATGAGAGTGCTCTGACTGAAAACCAAAAACTGAAA
ACAAAACTTCAGGAAGCCCAGCTAGAGCTAGCAGATATAAAGTCCAAGCTTGAGAAGGTGGCCCAGCAG
AAACAAGAAAAGACCTCTGACCGATCATCAGTGCTGGAGATGGAGAAACGGGAGAGGCGAGCCTTGGAG
CGCAAAATGTCAGAAATGGAGGAAGAAATGAAGGTGTTAACAGAACTGAAATCCGACAACCAGAGGCTG
AAAGATGAAAATGGTGCCCTCATCAGAGTCATCAGCAAACTGTCCAAGTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_002481
Insert Size 2949 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002481.3
RefSeq Size 11108 bp
RefSeq ORF 2949 bp
Locus ID 4660
UniProt ID O60237
Domains ANK
Protein Families Druggable Genome
Protein Pathways Vascular smooth muscle contraction
MW 110.4 kDa
Gene Summary Myosin phosphatase is a protein complex comprised of three subunits: a catalytic subunit (PP1c-delta, protein phosphatase 1, catalytic subunit delta), a large regulatory subunit (MYPT, myosin phosphatase target) and small regulatory subunit (sm-M20). Two isoforms of MYPT have been isolated--MYPT1 and MYPT2, the first of which is widely expressed, and the second of which may be specific to heart, skeletal muscle, and brain. Each of the MYPT isoforms functions to bind PP1c-delta and increase phosphatase activity. This locus encodes both MYTP2 and M20. Alternatively spliced transcript variants encoding different isoforms have been identified. Related pseudogenes have been defined on the Y chromosome. [provided by RefSeq, Oct 2011]
Transcript Variant: This variant (1) lacks an alternate in-frame exon compared to variant 8. The resulting isoform (a, also known as the large myosin binding subunit) has the same N- and C-termini but is shorter compared to isoform h.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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