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ATP2B4 (NM_001684) Human Untagged Clone
CAT＃: SC309950
ATP2B4 (untagged)-Human ATPase, Ca++ transporting, plasma membrane 4 (ATP2B4), transcript variant 2
CNY 23,280.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC309950 representing NM_001684.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACGAACCCATCAGACCGTGTCTTGCCTGCCAACTCGATGGCCGAGAGCCGTGAAGGGGACTTTGGC TGCACAGTAATGGAACTGAGGAAGCTCATGGAGCTGCGTTCAAGGGATGCACTGACCCAGATTAATGTC CACTATGGAGGTGTACAGAATCTCTGCAGTAGACTGAAAACCTCCCCTGTGGAAGGTCTGTCTGGGAAC CCTGCAGATCTGGAGAAACGTAGGCAGGTGTTTGGACACAACGTGATCCCCCCCAAAAAGCCCAAGACT TTCTTAGAATTAGTGTGGGAAGCTCTTCAAGATGTCACGCTTATCATCCTGGAGATTGCAGCCATCATC TCCCTGGTCCTGTCCTTTTATCGCCCTGCTGGTGAAGAAAATGAACTGTGTGGTCAAGTCGCAACTACC CCAGAAGATGAAAATGAGGCACAAGCTGGCTGGATTGAGGGGGCAGCCATCCTTTTCTCAGTGATCATC GTGGTGTTAGTGACTGCCTTTAATGATTGGAGCAAAGAGAAGCAATTCCGGGGGCTGCAGTGCCGCATT GAACAGGAGCAAAAGTTCTCCATCATCCGAAACGGTCAACTCATCCAGCTCCCTGTGGCTGAGATTGTG GTTGGTGATATTGCCCAAGTCAAATACGGTGATCTGCTGCCTGCAGATGGAATCCTGATCCAAGGGAAT GATCTGAAGATTGATGAGAGCTCTCTGACAGGGGAATCTGACCATGTCAAGAAGTCCCTGGACAAAGAC CCCATGTTGCTCTCAGGGACCCATGTCATGGAAGGTTCTGGCCGGATGGTGGTGACAGCTGTTGGTGTC AACTCTCAGACTGGAATCATCCTTACTCTCTTGGGGGTCAATGAGGATGACGAAGGGGAGAAAAAGAAG AAAGGTAAAAAACAAGGAGTCCCTGAAAATCGCAACAAAGCAAAGACCCAAGACGGAGTGGCCCTGGAA ATCCAGCCACTCAACAGCCAGGAGGGAATCGACAATGAGGAAAAGGACAAGAAGGCAGTCAAGGTGCCT AAAAAGGAGAAGTCAGTGCTGCAGGGCAAGCTGACTCGCCTGGCTGTTCAGATTGGGAAAGCCGGTCTG CTCATGTCTGCTCTCACGGTTTTCATCCTGATTCTATACTTTGTGATTGACAACTTTGTGATAAATCGC AGACCATGGCTCCCTGAGTGTACTCCCATCTACATCCAGTACTTTGTCAAGTTCTTCATCATCGGCATC ACTGTACTGGTGGTGGCTGTGCCAGAGGGGCTGCCTCTGGCTGTCACCATCTCACTGGCCTACTCTGTG AAGAAAATGATGAAAGACAATAACCTAGTACGGCACTTGGATGCTTGTGAGACCATGGGCAACGCCACC GCCATCTGCTCTGATAAGACAGGCACGTTGACCATGAACCGCATGACTGTGGTACAAGCTTATATTGGG GGCATCCATTACCGTCAAATCCCAAGCCCTGATGTCTTCCTGCCCAAAGTCCTGGACCTCATTGTCAAT GGCATTTCTATCAACAGTGCTTATACCTCCAAGATTCTGCCTCCAGAGAAGGAGGGAGGCCTGCCTCGG CAGGTGGGCAACAAGACCGAGTGTGCTCTGCTAGGCTTTGTCACAGATCTGAAGCAGGATTATCAGGCT GTGCGTAATGAAGTGCCCGAGGAGAAGCTCTACAAGGTGTACACCTTTAACTCAGTGCGCAAGTCAATG AGCACCGTCATCAGGAATCCCAACGGTGGCTTCCGTATGTACAGCAAGGGCGCCTCTGAGATCATCTTG CGCAAGTGTAATCGAATCCTGGACCGGAAAGGGGAAGCAGTGCCATTCAAGAATAAAGACAGAGATGAT ATGGTACGCACTGTCATCGAGCCCATGGCCTGTGATGGACTCCGGACTATCTGCATAGCTTACCGGGAC TTCGATGACACAGAGCCCTCTTGGGACAATGAGAATGAGATCCTCACCGAACTGACCTGTATCGCGGTG GTGGGCATTGAGGACCCTGTGCGCCCAGAGGTGCCAGATGCTATTGCCAAATGCAAACAAGCTGGCATT ACTGTCAGAATGGTGACAGGTGACAACATCAACACAGCCCGGGCCATTGCCACCAAATGTGGCATTCTG ACACCTGGGGATGACTTCCTGTGCTTAGAAGGCAAAGAATTCAACCGGCTCATCCGCAACGAGAAAGGC GAGGTAGAGCAAGAAAAGCTGGACAAGATCTGGCCTAAGCTTCGGGTCCTGGCGCGATCTTCTCCCACT GACAAGCACACCCTGGTGAAAGGCATAATTGACAGCACTGTTGGGGAACACCGGCAGGTCGTGGCTGTC ACTGGTGATGGCACAAATGACGGGCCTGCTCTGAAGAAAGCGGATGTTGGTTTTGCCATGGGCATCGCA GGCACAGATGTAGCAAAGGAGGCTTCAGACATCATCCTAACAGATGACAACTTCACCAGCATTGTGAAG GCAGTGATGTGGGGACGAAATGTCTATGACAGCATCTCCAAGTTCCTGCAGTTCCAGCTCACTGTCAAT GTGGTGGCCGTGATTGTAGCCTTCACTGGAGCCTGTATCACTCAGGATTCCCCATTGAAAGCTGTGCAG ATGTTGTGGGTTAATCTGATCATGGACACTTTTGCTTCATTGGCCCTGGCCACAGAGCCCCCTACGGAA TCTCTGTTGAAGCGGCGCCCCTATGGCCGAAATAAGCCTCTGATCTCACGCACTATGATGAAGAACATC TTGGGCCATGCATTCTATCAGCTCATTGTCATCTTTATCCTTGTCTTTGCGGGTGAGAAATTCTTTGAT ATTGATAGTGGGAGGAAGGCACCTCTACATTCACCACCCAGCCAGCACTATACCATTGTTTTTAACACC TTCGTGCTGATGCAGCTCTTCAATGAAATCAACTCCCGAAAGATCCATGGAGAGAAGAACGTCTTTTCA GGCATCTACCGCAACATTATCTTCTGCTCTGTAGTCTTGGGCACATTCATCTGCCAGATTTTCATCGTG GAATTTGGGGGTAAACCCTTCAGTTGTACAAGCCTCAGCCTGTCTCAGTGGCTGTGGTGTCTCTTCATT GGGATTGGAGAACTTCTGTGGGGCCAGTTCATCTCCGCAATACCTACCCGATCCCTGAAGTTCCTGAAG GAGGCTGGGCATGGCACCACCAAAGAGGAGATCACCAAGGATGCCGAGGGACTGGATGAGATTGACCAT GCTGAGATGGAGCTGCGCCGAGGCCAGATCCTCTGGTTCCGGGGCCTGAACCGTATCCAGACTCAGATC AAAGTGGTCAAAGCGTTCCATAGTTCCCTCCACGAAAGCATTCAGAAACCCTACAACCAAAAGTCCATC CACAGCTTCATGACCCACCCTGAATTCGCCATAGAGGAGGAGTTGCCACGAACACCACTCCTGGATGAG GAAGAGGAGGAAAATCCTGACAAGGCTTCTAAGTTTGGGACTAGGGTGCTCCTGTTGGATGGTGAGGTC ACTCCATATGCCAATACAAACAACAATGCGGTGGATTGCAACCAAGTGCAGCTCCCCCAGTCGGACAGC TCTCTACAGAGCCTAGAGACATCAGTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001684 |
| Insert Size | 3618 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001684.4 |
| RefSeq Size | 8753 bp |
| RefSeq ORF | 3618 bp |
| Locus ID | 493 |
| UniProt ID | P23634 |
| Domains | E1-E2_ATPase, Cation_ATPase_N, Hydrolase, Cation_ATPase_C |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Calcium signaling pathway |
| MW | 133.9 kDa |
| Gene Summary | The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks an alternate coding exon compared to variant 1, that causes a frameshift. The resulting isoform (4b) has a longer and distinct C-terminus compared to isoform 4a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213835 | ATP2B4 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 4 (ATP2B4), transcript variant 2 |
CNY 8,392.00 |
|
| RC213835L1 | Lenti ORF clone of Human ATPase, Ca++ transporting, plasma membrane 4 (ATP2B4), transcript variant 2, Myc-DDK-tagged |
CNY 10,792.00 |
|
| RC213835L2 | Lenti ORF clone of Human ATPase, Ca++ transporting, plasma membrane 4 (ATP2B4), transcript variant 2, mGFP tagged |
CNY 11,500.00 |
|
| RC213835L3 | Lenti ORF clone of Human ATPase, Ca++ transporting, plasma membrane 4 (ATP2B4), transcript variant 2, Myc-DDK-tagged |
CNY 10,792.00 |
|
| RC213835L4 | Lenti ORF clone of Human ATPase, Ca++ transporting, plasma membrane 4 (ATP2B4), transcript variant 2, mGFP tagged |
CNY 11,500.00 |
|
| RG213835 | ATP2B4 (tGFP-tagged) - Human ATPase, Ca++ transporting, plasma membrane 4 (ATP2B4), transcript variant 2 |
CNY 10,550.00 |
