ATP2B1 (NM_001682) Human Untagged Clone

CAT#: SC309942

ATP2B1 (untagged)-Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 2



  "NM_001682" in other vectors (4)

CNY 23,560.00


货期*
2周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Goat Polyclonal Antibody against ATP2B1 (aa312-327)
    • 100 ug

CNY 5,371.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms PMCA1; PMCA1kb
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_001682 edited
GCCACCATGGGCGACATGGCAAACAACTCAGTTGCTTACAGTGGTGTGAAAAACTCTTTG
AAGGAAGCTAATCATGATGGAGACTTTGGAATTACGCTCGCAGAGCTGCGGGCTCTCATG
GAGCTCAGGTCCACAGATGCATTACGAAAAATACAGGAAAGCTATGGAGATGTCTATGGA
ATTTGCACCAAATTGAAAACATCTCCCAATGAAGGTTTAAGTGGAAACCCTGCAGATTTA
GAAAGAAGAGAAGCAGTGTTTGGAAAGAATTTTATACCTCCTAAAAAGCCAAAAACCTTT
CTTCAATTAGTATGGGAAGCATTACAAGATGTCACTTTAATTATATTAGAAATTGCAGCC
ATAGTATCATTGGGCCTTTCTTTTTATCAGCCTCCAGAAGGGGATAATGCACTTTGTGGA
GAAGTTTCTGTTGGGGAGGAAGAAGGTGAAGGTGAAACTGGTTGGATTGAAGGAGCTGCA
ATCCTCTTGTCTGTAGTGTGTGTGGTGTTAGTAACAGCTTTCAATGACTGGAGTAAGGAA
AAACAGTTTAGAGGTTTGCAGAGCCGAATTGAACAAGAACAGAAGTTCACTGTCATCAGG
GGTGGTCAGGTCATTCAGATACCTGTAGCTGACATTACTGTTGGAGATATTGCTCAAGTG
AAATATGGTGATCTTCTTCCAGCTGACGGCATACTTATTCAAGGCAACGATCTTAAAATT
GATGAAAGCTCATTGACTGGTGAATCAGATCATGTTAAAAAGTCTTTAGATAAGGATCCC
TTACTTCTATCAGGTACTCATGTAATGGAAGGCTCTGGAAGAATGGTAGTTACAGCTGTA
GGTGTAAATTCTCAAACTGGAATTATCTTTACCTTACTTGGAGCTGGAGGTGAAGAGGAA
GAGAAGAAAGATGAGAAGAAAAAGGAAAAGAAAAATAAGAAACAAGATGGAGCTATTGAG
AATCGCAACAAAGCAAAAGCCCAGGATGGTGCAGCCATGGAAATGCAGCCATTGAAGAGT
GAAGAAGGTGGAGATGGTGATGAAAAAGATAAAAAGAAAGCAAATTTGCCAAAAAAGGAA
AAATCTGTTTTACAAGGGAAACTTACAAAACTGGCTGTTCAGATTGGCAAAGCAGGTCTG
TTGATGTCTGCCATCACAGTTATCATTCTAGTATTATATTTTGTCATTGACACCTTCTGG
GTTCAGAAAAGACCATGGCTTGCTGAGTGCACACCAATTTATATACAATACTTTGTGAAG
TTCTTCATTATTGGAGTTACAGTTTTAGTGGTCGCAGTGCCAGAAGGTCTTCCACTTGCA
GTCACGATCTCACTGGCTTATTCAGTCAAAAAAATGATGAAAGATAATAACTTAGTAAGG
CATCTGGATGCTTGTGAAACCATGGGAAATGCTACAGCTATTTGTTCAGATAAAACAGGA
ACTTTGACAATGAACAGAATGACAGTCGTTCAAGCTTACATAAATGAAAAACATTATAAA
AAGGTTCCTGAACCAGAAGCTATTCCACCAAATATTTTGTCCTATCTTGTAACAGGAATT
TCTGTGAATTGTGCTTATACATCAAAAATATTGCCACCAGAGAAAGAGGGTGGATTACCT
CGTCACGTTGGTAATAAAACTGAATGTGCCTTGTTGGGACTTCTTTTGGATTTAAAACGG
GATTATCAGGATGTTAGAAATGAAATACCAGAAGAAGCACTGTACAAAGTCTACACCTTC
AATTCTGTTAGGAAGTCCATGAGTACTGTCCTGAAAAATTCAGATGGAAGTTATCGAATA
TTCAGCAAGGGTGCATCTGAGATAATTCTGAAAAAGTGTTTCAAAATCTTGAGTGCTAAT
GGTGAGGCAAAAGTATTCAGACCAAGGGACCGTGATGATATTGTAAAAACTGTGATTGAA
CCGATGGCATCAGAAGGCTTGAGAACCATATGTCTTGCATTCAGAGATTTTCCAGCAGGA
GAACCAGAACCAGAGTGGGATAATGAAAATGATATTGTCACCGGCCTTACATGCATTGCT
GTTGTGGGGATTGAAGATCCTGTGAGACCTGAGGTGCCAGATGCAATTAAAAAGTGTCAG
AGGGCTGGAATTACTGTGCGGATGGTCACTGGTGATAATATTAATACTGCTCGGGCCATT
GCTACCAAATGTGGTATTTTACATCCTGGGGAAGATTTTCTGTGCCTAGAAGGTAAAGAT
TTTAACAGAAGAATACGAAATGAAAAAGGAGAGATTGAGCAAGAGAGGATAGACAAGATT
TGGCCAAAACTTCGAGTACTTGCAAGATCATCTCCTACTGATAAGCATACACTGGTTAAA
GGTATAATTGACAGCACTGTCTCAGACCAACGCCAGGTTGTAGCTGTAACTGGTGATGGT
ACAAATGATGGCCCAGCACTAAAGAAAGCAGATGTTGGATTTGCAATGGGTATTGCTGGA
ACTGATGTAGCTAAAGAAGCATCCGATATTATTCTCACAGATGACAACTTTACAAGCATT
GTTAAAGCAGTTATGTGGGGACGAAATGTCTATGACAGCATCTCAAAATTCCTTCAGTTC
CAACTTACTGTTAATGTAGTAGCAGTGATTGTTGCTTTTACGGGCGCCTGCATTACTCAA
GACTCACCGCTTAAGGCTGTGCAGATGCTGTGGGTAAACCTCATAATGGATACACTCGCT
TCCCTGGCTCTGGCAACGGAACCACCCACTGAGTCTCTCTTGCTTCGGAAACCTTATGGT
AGAAATAAGCCTCTCATCTCACGTACAATGATGAAGAATATTTTGGGTCATGCATTCTAT
CAACTTGTAGTAGTCTTTACACTCTTATTTGCTGGAGAAAAGTTTTTTGACATTGATAGT
GGAAGAAATGCTCCTTTGCATGCTCCTCCTTCAGAACATTATACTATTGTTTTTAATACC
TTTGTGCTGATGCAACTTTTCAACGAAATAAATGCCCGGAAAATTCATGGTGAAAGAAAT
GTATTCGAAGGAATCTTTAACAATGCCATCTTCTGCACAATTGTTTTAGGCACTTTTGTG
GTACAGATAATAATTGTGCAGTTTGGTGGAAAACCTTTCAGTTGTTCAGAACTTTCAATA
GAACAGTGGCTATGGTCAATATTCCTAGGAATGGGAACATTACTCTGGGGCCAGCTTATT
TCAACAATTCCAACTAGCCGTTTAAAATTCCTCAAAGAAGCTGGTCATGGAACACAAAAG
GAAGAAATACCTGAGGAGGAATTAGCAGAGGATGTTGAAGAGATTGATCACGCTGAAAGG
GAGTTGCGGCGTGGCCAAATCTTGTGGTTTAGAGGTCTGAACAGAATCCAAACACAGATT
CGAGTGGTGAATGCATTTCGTAG
Restriction Sites Please inquire     
ACCN NM_001682
Insert Size 3300 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001682.2, NP_001673.2
RefSeq Size 6796 bp
RefSeq ORF 3663 bp
Locus ID 490
UniProt ID P20020
Domains E1-E2_ATPase, Cation_ATPase_N, Hydrolase, Cation_ATPase_C
Protein Families Druggable Genome, Transmembrane
Protein Pathways Calcium signaling pathway
Gene Summary The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (2) lacks a segment in the coding region, which leads to a frameshift, compared to variant 1. The resulting isoform (1b) contains a longer and distinct C-terminus compared to isoform 1a. Variants 2, 3, 4, and 5 all encode the same isoform (1b). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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