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ATP2B1 (NM_001682) Human Untagged Clone
CAT＃: SC309942
ATP2B1 (untagged)-Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 2
CNY 23,560.00
货期*
2周
规格
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经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PMCA1; PMCA1kb |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_001682 edited
GCCACCATGGGCGACATGGCAAACAACTCAGTTGCTTACAGTGGTGTGAAAAACTCTTTG AAGGAAGCTAATCATGATGGAGACTTTGGAATTACGCTCGCAGAGCTGCGGGCTCTCATG GAGCTCAGGTCCACAGATGCATTACGAAAAATACAGGAAAGCTATGGAGATGTCTATGGA ATTTGCACCAAATTGAAAACATCTCCCAATGAAGGTTTAAGTGGAAACCCTGCAGATTTA GAAAGAAGAGAAGCAGTGTTTGGAAAGAATTTTATACCTCCTAAAAAGCCAAAAACCTTT CTTCAATTAGTATGGGAAGCATTACAAGATGTCACTTTAATTATATTAGAAATTGCAGCC ATAGTATCATTGGGCCTTTCTTTTTATCAGCCTCCAGAAGGGGATAATGCACTTTGTGGA GAAGTTTCTGTTGGGGAGGAAGAAGGTGAAGGTGAAACTGGTTGGATTGAAGGAGCTGCA ATCCTCTTGTCTGTAGTGTGTGTGGTGTTAGTAACAGCTTTCAATGACTGGAGTAAGGAA AAACAGTTTAGAGGTTTGCAGAGCCGAATTGAACAAGAACAGAAGTTCACTGTCATCAGG GGTGGTCAGGTCATTCAGATACCTGTAGCTGACATTACTGTTGGAGATATTGCTCAAGTG AAATATGGTGATCTTCTTCCAGCTGACGGCATACTTATTCAAGGCAACGATCTTAAAATT GATGAAAGCTCATTGACTGGTGAATCAGATCATGTTAAAAAGTCTTTAGATAAGGATCCC TTACTTCTATCAGGTACTCATGTAATGGAAGGCTCTGGAAGAATGGTAGTTACAGCTGTA GGTGTAAATTCTCAAACTGGAATTATCTTTACCTTACTTGGAGCTGGAGGTGAAGAGGAA GAGAAGAAAGATGAGAAGAAAAAGGAAAAGAAAAATAAGAAACAAGATGGAGCTATTGAG AATCGCAACAAAGCAAAAGCCCAGGATGGTGCAGCCATGGAAATGCAGCCATTGAAGAGT GAAGAAGGTGGAGATGGTGATGAAAAAGATAAAAAGAAAGCAAATTTGCCAAAAAAGGAA AAATCTGTTTTACAAGGGAAACTTACAAAACTGGCTGTTCAGATTGGCAAAGCAGGTCTG TTGATGTCTGCCATCACAGTTATCATTCTAGTATTATATTTTGTCATTGACACCTTCTGG GTTCAGAAAAGACCATGGCTTGCTGAGTGCACACCAATTTATATACAATACTTTGTGAAG TTCTTCATTATTGGAGTTACAGTTTTAGTGGTCGCAGTGCCAGAAGGTCTTCCACTTGCA GTCACGATCTCACTGGCTTATTCAGTCAAAAAAATGATGAAAGATAATAACTTAGTAAGG CATCTGGATGCTTGTGAAACCATGGGAAATGCTACAGCTATTTGTTCAGATAAAACAGGA ACTTTGACAATGAACAGAATGACAGTCGTTCAAGCTTACATAAATGAAAAACATTATAAA AAGGTTCCTGAACCAGAAGCTATTCCACCAAATATTTTGTCCTATCTTGTAACAGGAATT TCTGTGAATTGTGCTTATACATCAAAAATATTGCCACCAGAGAAAGAGGGTGGATTACCT CGTCACGTTGGTAATAAAACTGAATGTGCCTTGTTGGGACTTCTTTTGGATTTAAAACGG GATTATCAGGATGTTAGAAATGAAATACCAGAAGAAGCACTGTACAAAGTCTACACCTTC AATTCTGTTAGGAAGTCCATGAGTACTGTCCTGAAAAATTCAGATGGAAGTTATCGAATA TTCAGCAAGGGTGCATCTGAGATAATTCTGAAAAAGTGTTTCAAAATCTTGAGTGCTAAT GGTGAGGCAAAAGTATTCAGACCAAGGGACCGTGATGATATTGTAAAAACTGTGATTGAA CCGATGGCATCAGAAGGCTTGAGAACCATATGTCTTGCATTCAGAGATTTTCCAGCAGGA GAACCAGAACCAGAGTGGGATAATGAAAATGATATTGTCACCGGCCTTACATGCATTGCT GTTGTGGGGATTGAAGATCCTGTGAGACCTGAGGTGCCAGATGCAATTAAAAAGTGTCAG AGGGCTGGAATTACTGTGCGGATGGTCACTGGTGATAATATTAATACTGCTCGGGCCATT GCTACCAAATGTGGTATTTTACATCCTGGGGAAGATTTTCTGTGCCTAGAAGGTAAAGAT TTTAACAGAAGAATACGAAATGAAAAAGGAGAGATTGAGCAAGAGAGGATAGACAAGATT TGGCCAAAACTTCGAGTACTTGCAAGATCATCTCCTACTGATAAGCATACACTGGTTAAA GGTATAATTGACAGCACTGTCTCAGACCAACGCCAGGTTGTAGCTGTAACTGGTGATGGT ACAAATGATGGCCCAGCACTAAAGAAAGCAGATGTTGGATTTGCAATGGGTATTGCTGGA ACTGATGTAGCTAAAGAAGCATCCGATATTATTCTCACAGATGACAACTTTACAAGCATT GTTAAAGCAGTTATGTGGGGACGAAATGTCTATGACAGCATCTCAAAATTCCTTCAGTTC CAACTTACTGTTAATGTAGTAGCAGTGATTGTTGCTTTTACGGGCGCCTGCATTACTCAA GACTCACCGCTTAAGGCTGTGCAGATGCTGTGGGTAAACCTCATAATGGATACACTCGCT TCCCTGGCTCTGGCAACGGAACCACCCACTGAGTCTCTCTTGCTTCGGAAACCTTATGGT AGAAATAAGCCTCTCATCTCACGTACAATGATGAAGAATATTTTGGGTCATGCATTCTAT CAACTTGTAGTAGTCTTTACACTCTTATTTGCTGGAGAAAAGTTTTTTGACATTGATAGT GGAAGAAATGCTCCTTTGCATGCTCCTCCTTCAGAACATTATACTATTGTTTTTAATACC TTTGTGCTGATGCAACTTTTCAACGAAATAAATGCCCGGAAAATTCATGGTGAAAGAAAT GTATTCGAAGGAATCTTTAACAATGCCATCTTCTGCACAATTGTTTTAGGCACTTTTGTG GTACAGATAATAATTGTGCAGTTTGGTGGAAAACCTTTCAGTTGTTCAGAACTTTCAATA GAACAGTGGCTATGGTCAATATTCCTAGGAATGGGAACATTACTCTGGGGCCAGCTTATT TCAACAATTCCAACTAGCCGTTTAAAATTCCTCAAAGAAGCTGGTCATGGAACACAAAAG GAAGAAATACCTGAGGAGGAATTAGCAGAGGATGTTGAAGAGATTGATCACGCTGAAAGG GAGTTGCGGCGTGGCCAAATCTTGTGGTTTAGAGGTCTGAACAGAATCCAAACACAGATT CGAGTGGTGAATGCATTTCGTAG |
| Restriction Sites | Please inquire |
| ACCN | NM_001682 |
| Insert Size | 3300 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001682.2, NP_001673.2 |
| RefSeq Size | 6796 bp |
| RefSeq ORF | 3663 bp |
| Locus ID | 490 |
| UniProt ID | P20020 |
| Domains | E1-E2_ATPase, Cation_ATPase_N, Hydrolase, Cation_ATPase_C |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Calcium signaling pathway |
| Gene Summary | The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks a segment in the coding region, which leads to a frameshift, compared to variant 1. The resulting isoform (1b) contains a longer and distinct C-terminus compared to isoform 1a. Variants 2, 3, 4, and 5 all encode the same isoform (1b). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212619 | ATP2B1 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 2. Note: ORF is codon optimized |
CNY 12,744.00 |
|
| RC212619L3 | Lenti-ORF clone of ATP2B1 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 2. Note: ORF is codon optimized |
CNY 14,060.00 |
|
| RC212619L4 | Lenti-ORF clone of ATP2B1 (mGFP-tagged)-Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 2. Note: ORF is codon optimized |
CNY 14,060.00 |
|
| RG212619 | ATP2B1 (tGFP-tagged) - Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 2. Note: ORF is codon optimized |
CNY 13,400.00 |
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