AFAP (AFAP1) (NM_198595) Human Untagged Clone

CAT#: SC309818

AFAP1 (untagged)-Human actin filament associated protein 1 (AFAP1), transcript variant 2



  "NM_198595" in other vectors (4)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
AFAP1 mouse monoclonal antibody, clone OTI2G9 (formerly 2G9)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms AFAP; AFAP-110; AFAP110
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC309818 representing NM_198595.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGAAGAGTTAATAGTTGAACTTCGTCTCTTTCTTGAACTCCTGGACCATGAATATCTAACCTCAACT
GTCAGGGAGAAAAAGGCAGTGATAACCAACATTCTGCTAAGAATACAGTCATCCAAAGGTTTTGATGTG
AAGGACCATGCTCAGAAGCAGGAGACCGCTAACAGCCTGCCAGCCCCTCCTCAGATGCCCCTGCCGGAG
ATCCCTCAGCCCTGGCTGCCTCCTGACAGTGGGCCTCCACCATTGCCAACATCCTCCCTCCCAGAAGGT
TATTATGAGGAAGCTGTGCCGCTGAGCCCCGGAAAAGCTCCGGAATACATCACATCAAATTATGATTCC
GATGCGATGAGCAGCTCTTATGAGTCGTATGATGAAGAGGAGGAGGATGGGAAGGGGAAGAAAACCCGG
CACCAGTGGCCCTCCGAGGAGGCCTCCATGGACCTGGTCAAGGACGCCAAAATCTGCGCCTTCCTGCTG
CGGAAGAAGCGGTTCGGCCAGTGGACCAAGTTGCTCTGCGTCATCAAAGACACCAAACTGCTGTGCTAT
AAAAGTTCCAAGGACCAGCAGCCTCAGATGGAACTGCCACTCCAAGGCTGTAACATTACGTACATCCCG
AAAGACAGCAAAAAGAAGAAGCACGAGCTGAAGATTACTCAGCAGGGCACGGACCCGCTTGTTCTCGCC
GTCCAGAGCAAGGAACAGGCCGAGCAGTGGCTGAAGGTGATCAAAGAAGCCTACAGTGGTTGTAGTGGC
CCCGTGGATTCAGAGTGTCCTCCTCCACCAAGCTCCCCGGTGCACAAGGCAGAACTGGAGAAGAAACTG
TCTTCAGAGAGACCCAGCTCAGATGGGGAGGGTGTTGTGGAAAATGGAATTACCACATGTAATGGAAAG
GAGCAAGTGAAGAGGAAGAAAAGTTCCAAATCAGAGGCCAAGGGCACTGTGTCGAAAGTCACTGGGAAA
AAAATCACCAAGATCATCAGTCTGGGAAAGAAAAAGCCGTCCACAGACGAGCAGACCTCCTCAGCTGAG
GAAGATGTTCCCACCTGCGGCTATCTGAACGTGCTCTCCAACAGCCGCTGGCGAGAGCGCTGGTGCCGA
GTGAAAGATAACAAGCTCATTTTCCACAAGGACAGGACCGACCTGAAGACCCATATTGTGTCTATTCCG
CTCCGTGGCTGCGAGGTGATCCCGGGTTTGGATTCTAAACATCCTCTGACGTTCCGGCTGCTGCGCAAC
GGCCAGGAGGTTGCAGTATTGGAGGCATCTTCTTCTGAAGACATGGGCAGGTGGATTGGGATTTTACTC
GCAGAGACGGGATCGTCCACAGACCCGGAGGCTCTGCACTATGACTACATTGATGTGGAGATGTCTGCA
AGTGTCATTCAGACAGCCAAACAGACCTTCTGTTTCATGAACAGGCGTGTTATATCTGCTAACCCATAT
CTAGGGGGCACCTCCAACGGCTATGCCCACCCCAGCGGGACGGCACTTCATTATGACGATGTCCCGTGC
ATCAACGGCTCGCTCAAGGGTAAAAAGCCCCCCGTGGCGTCTAATGGGGTCACAGGAAAAGGGAAGACT
CTGAGCAGTCAGCCAAAGAAAGCGGATCCCGCGGCTGTTGTGAAAAGGACGGGTTCGAATGCTGCCCAG
TACAAGTATGGCAAGAACCGGGTAGAAGCAGATGCCAAGCGGCTACAGACCAAAGAGGAGGAGCTGCTG
AAGAGGAAAGAGGCCCTGCGGAATAGGCTGGCCCAGCTCCGCAAGGAAAGAAAAGACCTTCGAGCGGCT
ATTGAAGTGAACGCCGGCAGGAAGCCGCAGGCGATCCTGGAGGAGAAGCTGAAGCAGCTGGAGGAGGAG
TGCCGGCAGAAGGAGGCGGAGCGTGTCAGCCTGGAGCTGGAGCTGACGGAGGTCAAGGAGAGCCTGAAG
AAAGCGCTGGCGGGCGGAGTCACCCTGGGGCTGGCCATCGAGCCCAAGTCAGGGACATCGAGTCCACAG
TCTCCAGTGTTCCGGCACCGGACCCTGGAAAACTCGCCCATCTCCAGCTGTGACACCAGTGACACCGAG
GGCCCCGTGCCGGTGAACAGCGCGGCCGTCTTGAAGAAGAGCCAGGCTGCCCCGGGCAGCTCCCCCTGC
CGAGGGCATGTGCTGCGGAAGGCCAAGGAATGGGAATTGAAGAACGGGACCTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_198595
Insert Size 2193 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_198595.2
RefSeq Size 7534 bp
RefSeq ORF 2193 bp
Locus ID 60312
UniProt ID Q8N556
MW 80.7 kDa
Gene Summary The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
Transcript Variant: This variant (2) contains an alternate in-frame exon in the 3' coding region, compared to variant A. Variant 2 encodes a shorter protein (isoform B), compared to isoform A.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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