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SMURF1 (NM_020429) Human Untagged Clone
CAT＃: SC309681
SMURF1 (untagged)-Human SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1
CNY 5,552.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_020429 edited
ATGTCGAACCCCGGGACACGCAGGAACGGCTCCAGCATCAAGATCCGTCTGACAGTGTTA TGTGCCAAGAACCTTGCAAAGAAAGACTTCTTCAGGCTCCCTGACCCTTTTGCAAAGATT GTCGTGGATGGGTCTGGGCAGTGCCACTCAACCGACACTGTGAAAAACACATTGGACCCA AAGTGGAACCAGCACTATGATCTATATGTTGGGAAAACGGATTCGATAACCATTAGCGTG TGGAACCATAAGAAAATTCACAAGAAACAGGGAGCTGGCTTCCTGGGCTGTGTGCGGCTG CTCTCCAATGCCATCAGCAGATTAAAAGATACCGGATACCAGCGTTTGGATCTATGCAAA CTAAACCCCTCAGATACTGATGCAGTTCGTGGCCAGATAGTGGTCAGTTTACAGACACGA GACAGAATAGGAACCGGCGGCTCGGTGGTGGACTGCAGAGGACTGTTAGAAAATGAAGGA ACGGTGTATGAAGACTCGGGGCCTGGGAGGCCGCTCAGCTGCTTCATGGAGGAACCAGCC CCTTACACAGATAGCACCGGTGCTGCTGCTGGAGGAGGGAATTGCAGGTTCGTGGAGTCC CCAAGTCAAGATCAAAGACTTCAGGCACAGCGGCTTCGAAACCCTGATGTGCGAGGTTCA CTACAGACGCCCCAGAACCGACCACACGGCCACCAGTCCCCGGAACTGCCCGAAGGCTAC GAACAAAGAACAACAGTCCAGGGCCAAGTTTACTTTTTGCATACACAGACTGGAGTTAGC ACGTGGCACGACCCCAGGATACCAAGTCCCTCGGGGACCATTCCTGGGGGAGATGCAGCT TTTCTATACGAATTCCTTCTACAAGGCCATACATCTGAGCCCAGAGACCTTAACAGTGTG AACTGTGATGAACTTGGACCACTGCCGCCAGGCTGGGAAGTCAGAAGTACAGTTTCTGGG AGGATATATTTTGTAGATCATAATAACCGAACAACCCAGTTTACAGACCCAAGGTTACAC CACATCATGAATCACCAGTGCCAACTCAAGGAGCCCAGCCAGCCGCTGCCACTGCCCAGT GAGGGCTCTCTGGAGGACGAGGAGCTTCCTGCCCAGAGATACGAAAGAGATCTAGTCCAG AAGCTGAAAGTCCTCAGACACGAACTGTCGCTTCAGCAGCCCCAAGCTGGTCATTGCCGC ATCGAAGTGTCCAGAGAAGAAATCTTTGAGGAGTCTTACCGCCAGATAATGAAGATGCGA CCGAAAGACTTGAAAAAACGGCTGATGGTGAAATTCCGTGGGGAAGAAGGTTTGGATTAC GGTGGTGTGGCCAGGGAGTGGCTTTACTTGCTGTGCCATGAAATGCTGAATCCTTATTAC GGGCTCTTCCAGTATTCTACGGACAATATTTACATGTTGCAAATAAATCCGGATTCTTCA ATCAACCCCGACCACTTGTCTTATTTCCACTTTGTGGGGCGGATCATGGGGCTGGCTGTG TTCCATGGACACTACATCAACGGGGGCTTCACAGTGCCCTTCTACAAGCAGCTGCTGGGG AAGCCCATCCAGCTCTCAGATCTGGAATCTGTGGACCCAGAGCTGCATAAGAGCTTGGTG TGGATCCTAGAGAACGACATCACGCCTGTACTGGACCACACCTTCTGCGTGGAACACAAC GCCTTCGGGCGGATCCTGCAGCATGAACTGAAACCCAATGGCAGAAATGTGCCAGTCACA GAGGAGAATAAGAAAGAATACGTCCGGTTGTATGTAAACTGGAGGTTTATGAGAGGAATC GAAGCCCAGTTCTTAGCTCTGCAGAAGGGGTTCAATGAGCTCATCCCTCAACATCTGCTG AAGCCTTTTGACCAGAAGGAACTGGAGCTGATCATAGGCGGCCTGGATAAAATAGACTTG AACGACTGGAAGTCGAACACGCGGCTGAAGCACTGTGTGGCCGACAGCAACATCGTGCGG TGGTTCTGGCAAGCGGTGGAGACGTTCGATGAAGAAAGGAGGGCCAGGCTCCTGCAGTTT GTGACTGGGTCCACGCGAGTCCCGCTCCAAGGCTTCAAGGCTTTGCAAGGTTCTACAGGC GCGGCAGGGCCCCGGCTGTTCACCATCCACCTGATAGACGCGAACACAGACAACCTTCCG AAGGCCCATACCTGCTTTAACCGGATCGACATTCCACCATATGAGTCCTATGAGAAGCTC TACGAGAAGCTGCTGACAGCCGTGGAGGAGACCTGCGGGTTTGCTGTGGAGTGA >OriGene 5' read for NM_020429 unedited
GTTGCATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGCTGGATCCG CGGCTGCCCGATCGTTGGCGGGAGATGTCGAACCCCGGGACACGCAGGAACGGCTCCAGC ATCAAGATCCGTCTGACAGTGTTATGTGCCAAGAACCTTGCAAAGAAAGACTTCTTCAGG CTCCCTGACCCTTTTGCAAAGATTGTCGTGGATGGGTCTGGGCAGTGCCACTCAACCGAC ACTGTGAAAAACACATTGGACCCAAAGTGGAACCAGCACTATGATCTATATGTTGGGAAA ACGGATTCGATAACCATTAGCGTGTGGAACCATAAGAAAATTCACAAGAAACAGGGAGCT GGCTTCCTGGGCTGTGTGCGGCTGCTCTCCAATGCCATCAGCAGATTAAAAGATACCGGA TACCAGCGTTTGGATCTATGCAAACTAAACCCCTCAGATACTGATGCAGTTCGTGGCCAG ATAGTGGTCAGTTTACAGACACGAGACAGAATAGGAACCGGCGGCTCGGTGGTGGACTGC AGAGGACTGTTAGAAAATGAAGGAACGGTGTATGAAGACTCGGGGCCTGGGAGGCCGCTC AGCTGCTTCATGGAGGAACCAGCCCCTTACACAGATAGCACCGGTGCTGCTGCTGGAGGA GGGAATTGCAGGTTCGTGGAGTCCCCAAGTCAAGATCAAAGACTTCAGGCACAGCGGCTT CGAAACCCTGATGTGCGAGGTTCACTACAGACGCCCCAAAACCCGACCACACGGCCACCA GTCCCCGGGACTGCCCCGAAGGCTACGAACAAAGAACAACAGTCCAGGG >OriGene 3' genomic read for NM_020429 unedited
ATAGGAGATGGCAATTCCAGGCCAGNAAAGCACTGGGGAGGGTCACAGGTGCCCCCGGGA TCTGTTAGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTT TTAAATGTAAAGTCGTGAGTTTATTGCATATGTAACAAAATGAACCTGACCTCCTGGGCC CAGCCTGCTGTACAATCACTGTTTGTTTTGTGTTTCCAGCTGGTTCCATAACCACATTAA ATAGAACTAGTATTTCATTAAATACTTTTGATTTTGACATAGAACATTAGTGTACAACTT TCACAAAATAAATCAGTGATAAAAACAGTGGGAAGGATAACAAGGATAGCAGCAATACTT CAAAACAAGACATTACAAAATAAATTAAAAAATACGTTATAAAGTGGTTGAGAAACAAAA ATAAACACATTTTTAAAATCCATACTATGTTTCGGGAANGCTGCCGTGTGGCACACACCT TGCTGCAAATGGCGGGTGGGGGGACATTTTCTCTGGAAAAAGCCTTTTCCTGAATTTTCG GGTTTGGTGAACCCCTTTAATCTGAAATTGGGACCATACGTCCGAAACAATCTTAGGGGG TTCCAAATTTCTTTTTGGTTGGGCAAAATTTTTTTTTTAAAAAATTTGAAAAAAACCCCT TTTTTCGGGGCCCCCCCGGGCCTTTCCAGGGGAGCCTCAAATTTTTTTCTAAATTTAAAA AAACTCTTGGGGGAAGAGGGGGGTTTAACAAACACCCCCCCCGGAGGATGGCGGGGCG |
| Restriction Sites | Please inquire |
| ACCN | NM_020429 |
| Insert Size | 5500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020429.1, NP_065162.1 |
| RefSeq Size | 5737 bp |
| RefSeq ORF | 2274 bp |
| Locus ID | 57154 |
| UniProt ID | Q9HCE7 |
| Protein Families | Druggable Genome |
| Protein Pathways | Endocytosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis |
| Gene Summary | This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Dec 2010] Transcript Variant: This variant (1) encodes the longer isoform (1) of this protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222902 | SMURF1 (Myc-DDK-tagged)-Human SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1 |
CNY 5,544.00 |
|
| RC222902L3 | Lenti ORF clone of Human SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC222902L4 | Lenti ORF clone of Human SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG222902 | SMURF1 (tGFP-tagged) - Human SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1 |
CNY 7,144.00 |
