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Calpain 10 (CAPN10) (NM_023085) Human Untagged Clone
CAT＃: SC309621
CAPN10 (untagged)-Human calpain 10 (CAPN10), transcript variant 3
CNY 8,360.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CANP10; NIDDM1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC309621 representing NM_023085.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCGGGCGGGCCGGGGCGCGACGCCGGCGAGGGAGCTGTTCCGGGACGCCGCCTTCCCCGCCGCGGAC TCCTCGCTCTTCTGCGACTTGTCTACGCCGCTGGCCCAGTTCCGCGAGGACATCACGTGGAGGCGGCCC CAGGAGATTTGTGCCACACCCCGGCTGTTTCCAGATGACCCACGGGAAGGGCAGGTGAAGCAGGGGCTG CTGGGGGATTGCTGGTTCCTGTGTGCCTGCGCCGCGCTGCAGAAGAGCAGGCACCTCCTGGACCAGGTC ATTCCTCCGGGACAGCCGAGCTGGGCCGACCAGGAGTACCGGGGCTCCTTCACCTGTCGCATTTGGCAG TTTGGACGCTGGGTGGAGGTGACCACAGATGACCGCCTGCCGTGCCTTGCAGGGAGACTCTGTTTCTCC CGCTGCCAGAGGGAGGATGTGTTCTGGCTCCCCTTACTGGAAAAGGTCTACGCCAAGGTCCATGGGTCC TACGAGCACCTGTGGGCCGGGCAGGTGGCGGATGCCCTGGTGGACCTGACCGGCGGCCTGGCAGAAAGA TGGAACCTGAAGGGCGTAGCAGGAAGCGGAGGCCAGCAGGACAGGCCAGGCCGCTGGGAGCACAGGACT TGTCGGCAGCTGCTCCACCTGAAGGACCAGTGTCTGATCAGCTGCTGCGTGCTCAGCCCCAGAGCAGGT GCCCGGGAGCTGGGGGAGTTCCATGCCTTCATTGTCTCGGACCTGCGGGAGCTCCAGGGTCAGGCGGGC CAGTGCATCCTGCTGCTGCGGATCCAGAACCCCTGGGGCCGGCGGTGCTGGCAGGGGCTCTGGAGAGAG GGGGGTGAAGGGTGGAGCCAGGTAGATGCAGCGGTAGCATCTGAGCTCCTGTCCCAGCTCCAGGAAGGG GAGTTCTGGGTGGAGGAGGAGGAGTTCCTCAGGGAGTTTGACGAGCTCACCGTTGGCTACCCGGTCACG GAGGCCGGCCACCTGCAGAGCCTCTACACAGAGAGGCTGCTCTGCCATACGCGGGCGCTGCCTGGGGCC TGGGTCAAGGGCCAGTCAGCAGGAGGCTGCCGGAACAACAGCGGCTTTCCCAGCAACCCCAAATTCTGG CTGCGGGTCTCAGAACCGAGTGAGGTGTACATTGCCGTCCTGCAGAGATCCAGGCTGCACGCGGCGGAC TGGGCAGGCCGGGCCCGGGCACTGGTGGGTGACAGTCATACTTCGTGGAGCCCAGCGAGCATCCCGGGC AAGCACTACCAGGCTGTGGGTCTGCACCTCTGGAAGGTCCCAGAGGGTGGAAGGAGCCAGGACGCACCC CCACTGCTGCTGCAGGAGCCGCTGCTGAGCTGCGTGCCACATCGCTACGCCCAGGAGGTGAGCCGGCTC TGCCTCCTGCCTGCGGGCACCTACAAGGTTGTGCCCTCCACCTACCTGCCGGACACAGAGGGGGCCTTC ACAGTGACCATCGCAACCAGGATTGACAGGCCATCCATTCACAGCCAGGAGATGCTGGGCCAGTTCCTC CAAGAGGTCTCCGTCATGGCAGTGATGAAAACCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_023085 |
| Insert Size | 1554 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_023085.3 |
| RefSeq Size | 2197 bp |
| RefSeq ORF | 1554 bp |
| Locus ID | 11132 |
| UniProt ID | Q9HC96 |
| Protein Families | Druggable Genome, Protease |
| MW | 58 kDa |
| Gene Summary | Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene. [provided by RefSeq, Sep 2010] Transcript Variant: This variant (3, also known as CAPN10c) lacks two consecutive exons, resulting in the loss of an in-frame segment in the 3' coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224179 | CAPN10 (Myc-DDK-tagged)-Human calpain 10 (CAPN10), transcript variant 3 |
CNY 3,848.00 |
|
| RC224179L3 | Lenti-ORF clone of CAPN10 (Myc-DDK-tagged)-Human calpain 10 (CAPN10), transcript variant 3 |
CNY 6,370.00 |
|
| RC224179L4 | Lenti-ORF clone of CAPN10 (mGFP-tagged)-Human calpain 10 (CAPN10), transcript variant 3 |
CNY 6,370.00 |
|
| RG224179 | CAPN10 (tGFP-tagged) - Human calpain 10 (CAPN10), transcript variant 3 |
CNY 4,940.00 |
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