DRIP130 (MED23) (NM_015979) Human Untagged Clone

CAT#: SC309598

MED23 (untagged)-Human mediator complex subunit 23 (MED23), transcript variant 2



  "NM_015979" in other vectors (6)

CNY 26,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal anti-MED23 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ARC130; CRSP3; CRSP130; CRSP133; DRIP130; MRT18; SUR-2; SUR2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC309598 representing NM_015979.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGAGACGCAACTGCAGAGCATTTTCGAAGAGGTGGTGAAAACGGAAGTTATAGAAGAGGCTTTTCCT
GGCATGTTTATGGATACTCCTGAAGATGAGAAAACAAAACTAATTAGCTGTTTGGGGGCCTTCAGACAG
TTTTGGGGTGGTCTTTCTCAGGAGTCTCATGAACAGTGTATCCAGTGGATTGTTAAGTTTATTCATGGT
CAGCATAGTCCTAAAAGAATTTCTTTTCTTTATGACTGCTTAGCAATGGCAGTTGAGACTGGTCTCCTT
CCACCCAGGCTGGTTTGTGAATCCCTGATAAACTCTGACACTCTTGAGTGGGAAAGAACACAGCTTTGG
GCCTTAACATTTAAACTGGTTCGGAAAATAATTGGGGGAGTGGATTACAAGGGTGTTCGAGATCTCTTA
AAAGTGATTTTGGAGAAGATTTTGACAATTCCTAATACAGTGAGCTCTGCTGTTGTACAGCAGCTTCTG
GCAGCAAGAGAGGTTATAGCATATATCTTGGAAAGAAATGCCTGCTTATTACCAGCCTATTTTGCAGTC
ACTGAGATCAGGAAACTGTATCCTGAAGGCAAACTTCCACACTGGTTACTTGGAAACCTAGTATCAGAC
TTTGTGGATACCTTCAGGCCCACAGCAAGGATAAACTCCATTTGTGGTCGCTGTAGTCTTCTGCCAGTT
GTAAATAATTCGGGTGCCATTTGTAATTCATGGAAACTGGATCCTGCTACTCTTCGTTTTCCTTTGAAA
GGCCTTTTGCCATATGATAAGGATCTGTTTGAACCACAGACTGCTTTGTTGAGATATGTATTGGAGCAG
CCTTATTCCAGGGATATGGTCTGCAATATGCTAGGTTTAAATAAGCAGACCTTGAACATTGCTCAGCAC
AAGCAGCGCTGCCCTGTGCTGGAGGACCAGTTGGTGGATCTGGTTGTTTATGCCATGGAGCGATCTGAG
ACCGAGGAGAAGTTTGACGATGGGGGAACAAGCCAACTCCTGTGGCAGCATCTCTCAAGTCAGCTCATT
TTCTTTGTGCTTTTCCAGTTTGCAAGTTTTCCACATATGGTGCTTTCTCTTCATCAGAAGTTAGCAGGG
CGAGGACTGATTAAAGGCAGAGATCATCTTATGTGGGTTCTCCTGCAATTCATTTCTGGAAGTATTCAG
AAAAATGCACTAGCTGATTTTCTCCCTGTGATGAAGCTCTTCGACTTGCTATACCCAGAAAAAGAATAT
ATCCCAGTTCCTGATATTAACAAACCCCAGTCAACCCATGCCTTTGCAATGACCTGTATTTGGATTCAT
CTCAATAGAAAAGCTCAAAATGACAACTCCAAGCTACAGATTCCAATACCTCATTCCCTAAGACTTCAC
CATGAGTTCCTGCAGCAGAGTCTAAGAAATAAAAGTTTACAGATGAATGACTATAAGATTGCTCTATTG
TGTAATGCATACTCTACAAATTCAGAATGTTTTACATTACCCATGGGAGCTCTGGTAGAAACTATTTAT
GGAAATGGAATTATGAGGATACCTCTCCCTGGAACAAACTGTATGGCTTCAGGATCTATTACCCCCTTA
CCTATGAACCTCCTGGATTCACTGACAGTTCATGCCAAAATGAGCCTTATTCACAGCATTGCAACCAGG
GTGATAAAACTTGCTCATGCAAAGTCCAGTGTGGCCTTGGCTCCAGCCCTAGTGGAAACTTACAGTCGT
TTATTGGTCTATATGGAAATAGAGTCTTTGGGCATCAAAGGATTTATCAGTCAGCTTTTGCCAACTGTT
TTCAAATCACATGCATGGGGGATCTTACACACACTCCTTGAGATGTTTAGCTACCGGATGCATCATATT
CAGCCTCATTACAGAGTTCAGCTCCTGAGTCATCTTCATACTTTGGCTGCAGTTGCACAAACAAACCAG
AACCAGCTCCATCTTTGTGTCGAGAGCACTGCTCTCAGGCTTATAACAGCATTAGGTAGCTCAGAGGTA
CAACCGCAGTTTACACGCTTCCTTAGTGATCCCAAAACAGTGCTCTCAGCAGAATCTGAAGAACTGAAC
CGAGCCTTGATATTGACCTTGGCTAGAGCAACTCATGTAACAGATTTTTTTACAGGCTCTGATTCAATT
CAGGGAACTTGGTGTAAAGACATACTTCAGACCATCATGAGTTTCACTCCTCATAATTGGGCTTCACAC
ACCCTGAGCTGTTTTCCAGGCCCACTACAGGCATTCTTCAAACAAAATAATGTGCCTCAGGAAAGCCGT
TTTAATCTGAAAAAAAATGTGGAGGAGGAGTATAGGAAGTGGAAGTCAATGAGCAACGAAAACGACATT
ATTACCCACTTCTCTATGCAGGGCTCCCCTCCTCTCTTTCTTTGTCTTCTCTGGAAAATGCTCTTGGAA
ACAGATCATATTAATCAGATTGGCTATAGAGTATTAGAGAGAATTGGAGCCAGGGCCTTGGTAGCCCAT
GTGAGGACATTTGCAGATTTCCTGGTATATGAGTTTTCTACATCAGCAGGGGGTCAGCAACTCAATAAA
TGCATTGAAATTCTTAATGACATGGTATGGAAGTATAACATTGTTACACTGGACAGATTAATTCTCTGC
CTGGCCATGCGTAGTCACGAAGGAAATGAAGCCCAGGTTTGTTATTTCATAATTCAGTTGCTGTTACTC
AAACCAAACGATTTTAGAAATCGAGTAAGTGACTTTGTGAAGGAAAATTCCCCAGAGCACTGGTTACAG
AATGACTGGCACACCAAGCACATGAATTATCACAAGAAATATCCAGAGAAGTTGTATTTTGAGGGCCTC
GCGGAACAGGTGGATCCTCCTGTACAGATCCAGTCTCCCTATCTGCCCATCTATTTTGGGAATGTGTGT
CTTCGATTCCTTCCAGTATTTGATATAGTAATCCACAGATTTTTAGAGTTGCTTCCGGTATCCAAATCA
CTGGAGACTCTACTGGATCATCTAGGAGGCTTATATAAATTTCATGATCGTCCAGTGACTTATCTGTAT
AACACTCTGCACTATTATGAAATGCACCTGAGAGACCGCGCATTTCTCAAACGAAAACTCGTCCATGCG
ATCATTGGCTCTCTGAAGGATAATCGACCGCAGGGCTGGTGTCTAAGTGACACTTACCTGAAATGCGCT
ATGAATGCACGAGAGGAAAATCCTTGGGTTCCAGATGACACCTACTATTGCAGATTGATTGGCAGACTA
GTCGATACGATGGCTGGCAAATCTCCTGGTCCCTTTCCAAACTGTGACTGGAGATTCAATGAGTTTCCC
AACCCAGCTGCCCATGCTCTCCATGTTACTTGTGTGGAGCTCATGGCCTTGGCAGTTTCAGGCAAAGAA
GTTGGGAATGCCCTTCTAAATGTTGTCCTAAAAAGTCAGCCTTTAGTGCCAAGAGAGAACATTACAGCA
TGGATGAATGCAATTGGTTTGATCATCACTGCCCTACCAGAGCCATATTGGATTGTTCTTCATGATCGA
ATTGTGAGTGTCATCAGCAGCCCCAGCTTGACGTCTGAAACAGAGTGGGTTGGCTATCCATTCCGCCTC
TTTGATTTCACTGCCTGTCATCAGTCCTACTCTGAGATGAGTTGTAGCTATACGTTAGCTCTTGCACAT
GCTGTGTGGCACCATTCTAGCATCGGACAACTTTCTCTCATTCCAAAGTTTCTTACTGAAGTACTTCTT
CCTATAGTGAAGACCGAATTCCAGTTGCTTTATGTATACCATCTTGTTGGACCATTTTTACAAAGATTT
CAGCAAGAGAGAACTCGTTGTATGATAGAGATTGGTGTGGCGTTTTATGACATGCTGCTGAATGTTGAC
CAGTGTAGCACCCATTTAAATTACATGGATCCCATCTGTGACTTCCTCTATCACATGAAGTATATGTTT
ACTGGTGACAGCGTGAAAGAGCAAGTAGAGAAGATTATCTGTAACTTAAAACCAGCTTTAAAACTTCGT
CTTCGATTCATCACACACATTAGCAAGATGGAGCCAGCTGCAGTGCCTCCACAAGCCATGAACAGTGGG
TCTCCAGCACCTCAGTCTAATCAGTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_015979
Insert Size 4098 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_015979.3
RefSeq Size 4630 bp
RefSeq ORF 4098 bp
Locus ID 9439
UniProt ID Q9ULK4
Protein Families Druggable Genome, Transcription Factors
MW 156.2 kDa
Gene Summary The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2012]
Transcript Variant: This variant (2) contains an alternate in-frame exon and uses an alternate splice junction in the 3' end compared to variant 1. The resulting isoform (b) contains an alternate short internal segment and is shorter at the C-terminus compared to isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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