United States
Germany
Japan
United Kingdom
China
Myosin light chain kinase (MYLK) (NM_053028) Human Untagged Clone
CAT＃: SC309520
MYLK (untagged)-Human myosin light chain kinase (MYLK), transcript variant 3B
CNY 12,064.00
货期*
现货
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210; MMIHS; MMIHS1; MSTP083; MYLK1; smMLCK |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_053028 edited
CATGGGGGATGTGAAGCTGGTTGCCTCGTCACACATTTCCAAAACCTCCCTCAGTGTGGA TCACTCAAGAGTTGACTCCATGCCCCTGACAGAGGCCCCTGCTTTCATTTTGCCCCCTCG GAACCTCTGCATCAAAGAAGGAGCCACCGCCAAGTTCGAAGGGCGGGTCCGGGGTTACCC AGAGCCCCAGGTGACATGGCACAGAAACGGGCAACCCATCACCAGCGGGGGCCGCTTCCT GCTGGATTGCGGCATCCGGGGGACTTTCAGCCTTGTGATTCATGCTGTCCATGAGGAGGA CAGGGGAAAGTATACCTGTGAAGCCACCAATGGCAGTGGTGCTCGCCAGGTGACAGTGGA GTTGACAGTAGAAGGAAGTTTTGCGAAGCAGCTTGGTCAGCCTGTTGTTTCCAAAACCTT AGGGGATAGATTTTCAGCTCCAGCAGTGGAGACCCGTCCTAGCATCTGGGGGGAGTGCCC ACCAAAGTTTGCTACCAAGCTGGGCCGAGTTGTGGTCAAAGAAGGACAGATGGGACGATT CTCCTGCAAGATCACTGGCCGGCCCCAACCGCAGGTCACCTGGCTCAAGGGAAATGTTCC ACTGCAGCCGAGTGCCCGTGTGTCTGTGTCTGAGAAGAACGGCATGCAGGTTCTGGAAAT CCATGGAGTCAACCAAGATGACGTGGGAGTGTACACGTGCCTGGTGGTGAACGGGTCGGG GAAGGCCTCGATGTCAGCTGAACTTTCCATCCAAGGTTTGGACAGTGCCAATAGGTCATT TGTGAGAGAAACAAAAGCCACCAATTCAGATGTCAGGAAAGAGGTGACCAATGTAATCTC AAAGGAGTCGAAGCTGGACAGTCTGGAGGCTGCAGCCAAAAGCAAGAACTGCTCCAGCCC CCAGAGAGGTGGCTCCCCACCCTGGGCTGCAAACAGCCAGCCTCAGCCCCCAAGGGAGTC CAAGCTGGAGTCATGCAAGGACTCGCCCAGAACGGCCCCGCAGACCCCGGTCCTTCAGAA GACTTCCAGCTCCATCACCCTGCAGGCCGCAAGAGTTCAGCCGGAACCAAGAGCACCAGG CCTGGGGGTCCTATCACCTTCTGGAGAAGAGAGGAAGAGGCCAGCTCCTCCCCGTCCAGC CACCTTCCCCACCAGGCAGCCTGGCCTGGGGAGCCAAGATGTTGTGAGCAAGGCTGCTAA CAGGAGAATCCCCATGGAGGGCCAGAGGGATTCAGCATTCCCCAAATTTGAGAGCAAGCC CCAAAGCCAGGAGGTCAAGGAAAATCAAACTGTCAAGTTCAGATGTGAAGGGCTTGCCGT GATGGAGGTGGCCCCCTCCTTCTCCAGTGTCCTGAAGGACTGCGCTGTTATTGAGGGCCA GGATTTTGTGCTGCAGTGCTCCGTACGGGGGACCCCAGTGCCCCGGATCACTTGGCTGCT GAATGGGCAGCCCATCCAGTACGCTCGCTCCACCTGCGAGGCCGGCGTGGCTGAGCTCCA CATCCAGGATGCCCTGCCGGAGGACCATGGCACCTACACCTGCCTAGCTGAGAATGCCTT GGGGCAGGTGTCCTGCAGCGCCTGGGTCACCGTCCATGAAAAGAAGAGTAGCAGGAAGAG TGAGTACCTTCTGCCTGTGGCTCCCAGCAAGCCCACTGCACCCATCTTCCTGCAGGGCCT CTCTGATCTCAAAGTCATGGATGGAAGCCAGGTCACTATGACTGTCCAAGTGTCAGGGAA TCCACCCCCTGAAGTCATCTGGCTGCACAATGGGAATGAGATCCAAGAGTCAGAGGACTT CCACTTTGAACAGAGAGGAACTCAGCACAGCCTTTGTATCCAGGAAGTGTTCCCGGAGGA CACGGGCACGTACACCTGCGAGGCCTGGAACAGCGCTGGAGAGGTCCGCACCCAGGCCGT GCTCACGGTACAAGAGCCTCACGATGGCACCCAGCCCTGGTTCATCAGTAAGCCTCGCTC AGTGACAGCCTCCCTGGGCCAGAGTGTCCTCATCTCCTGCGCCATAGCTGGTGACCCCTT TCCTACCGTGCACTGGCTCAGAGATGGCAAAGCCCTCTGCAAAGACACTGGCCACTTCGA GGTGCTTCAGAATGAGGACGTGTTCACCCTGGTTCTAAAGAAGGTGCAGCCCTGGCATGC CGGCCAGTATGAGATCCTGCTCAAGAACCGGGTTGGCGAATGCAGTTGCCAGGTGTCACT GATGCTACAGAACAGCTCTGCCAGAGCCCTTCCACGGGGGAGGGAGCCTGCCAGCTGCGA GGACCTCTGTGGTGGAGGAGTTGGTGCTGATGGTGGTGGTAGTGACTGCTATGGGTCCCT GAGGCCTGGCTGGCCAGCAAGAGGGCAGGGTTGGCCAGAGGAGGAAGACGGCGAGGACGT GCGAGGGGTGCTGAAGAGGCGCGTGGAGACGAGGCAGCACACTGAGGAGGCGATCCGCCA GCAGGAGGTGGAGCAGCTGGACTTCCGAGACCTCCTGGGGAAGAAGGTGAGTACAAAGAC CCTATCGGAAGACGAACTGAAGGAGATCCCAGCCGAGCAGATGGATTTCCGTGCCAACCT GCAGCGGCAAGTGAAGCCAAAGACTGTGTCTGAGGAAGAGAGGAAGGTGCACAGCCCCCA GCAGGTCGATTTTCGCTCTGTCCTGGCCAAGAAGGGGACTTCCAAGACCCCCGTGCCTGA GAAGGTGCCACCGCCAAAACCTGCCACCCCGGATTTTCGCTCAGTGCTGGGTGGCAAGAA GAAATTACCAGCAGAGAATGGCAGCAGCAGTGCCGAGACCCTGAATGCCAAGGCAGTGGA GAGTTCCAAGCCCCTGAGCAATGCACAGCCTTCAGGGCCCTTGAAACCCGTGGGCAACGC CAAGCCTGCTGAGACCCTGAAGCCAATGGGCAACGCCAAGCCTGCCGAGACCCTGAAGCC CATGGGCAATGCCAAGCCTGATGAGAACCTGAAATCCGCTAGCAAAGAAGAACTCAAGAA AGACGTTAAGAATGATGTGAACTGCAAGAGAGGCCATGCAGGGACCACAGATAATGAAAA GAGATCAGAGAGCCAGGGGACAGCCCCAGCCTTCAAGCAGAAGCTGCAAGATGTTCATGT GGCAGAGGGCAAGAAGCTGCTGCTCCAGTGCCAGGTGTCTTCTGACCCCCCAGCCACCAT CATCTGGACGCTGAATGGAAAGACCCTCAAGACCACCAAGTTCATCATCCTCTCCCAGGA AGGCTCACTCTGCTCCGTCTCCATCGAGAAGGCACTGCCTGAGGACAGAGGCTTATACAA GTGTGTAGCCAAGAATGACGCTGGCCAGGCGGAGTGCTCCTGCCAAGTCACCGTGGATGA TGCTCCAGCCAGTGAGAACACCAAGGCCCCAGAGATGAAATCCCGGAGGCCCAAGAGCTC TCTTCCTCCCGTGCTAGGAACTGAGAGTGATGCGACTGTGAAAAAGAAACCTGCCCCCAA GACACCTCCGAAGGCAGCAATGCCCCCTCAGATCATCCAGTTCCCTGAGGACCAGAAGGT ACGCGCAGGAGAGTCAGTGGAGCTGTTTGGCAAAGTGACAGGCACTCAGCCCATCACCTG TACCTGGATGAAGTTCCGAAAGCAGATCCAGGAAAGCGAGCACATGAAGGTGGAGAACAG CGAGAATGGCAGCAAGCTCACCATCCTGGCCGCGCGCCAGGAGCACTGCGGCTGCTACAC ACTGCTGGTGGAGAACAAGCTGGGCAGCAGGCAGGCCCAGGTCAACCTCACTGTCGTGGA TAAGCCAGACCCCCCAGCTGGCACACCTTGTGCCTCTGACATTCGGAGCTCCTCACTGAC CCTGTCCTGGTATGGCTCCTCATATGATGGGGGCAGTGCTGTACAGTCCTACAGCATCGA GATCTGGGACTCAGCCAACAAGACGTGGAAGGAACTAGCCACATGCCGCAGCACCTCTTT CAACGTCCAGGACCTGCTGCCTGACCACGAATATAAGTTCCGTGTACGTGCAATCAACGT GTATGGAACCAGTGAGCCAAGCCAGGAGTCTGAACTCACAACGGTAGGAGAGAAACCTGA AGAGCCGAAGGATGAAGTGGAGGTGTCAGACGATGATGAGAAGGAGCCCGAGGTTGATTA CCGGACAGTGACAATCAATACTGAACAAAAAGTATCTGACTTCTACGACATTGAGGAGAG ATTAGGATCTGGGAAATTTGGACAGGTCTTTCGACTTGTAGAAAAGAAAACTCGAAAAGT CTGGGCAGGGAAGTTCTTCAAGGCATATTCAGCAAAAGAGAAAGAGAATATCCGGCAGGA GATTAGCATCATGAACTGCCTCCACCACCCTAAGCTGGTCCAGTGTGTGGATGCCTTTGA AGAAAAGGCCAACATCGTCATGGTCCTGGAGATCGTGTCAGGAGGGGAGCTGTTTGAGCG CATCATTGACGAGGACTTTGAGCTGACGGAGCGTGAGTGCATCAAGTACATGCGGCAGAT CTCGGAGGGAGTGGAGTACATCCACAAGCAGGGCATCGTGCACCTGGACCTCAAGCCGGA GAACATCATGTGTGTCAACAAGACGGGCACCAGGATCAAGCTCATCGACTTTGGTCTGGC CAGGAGGCTGGAGAACGCGGGGTCTCTGAAGGTCCTCTTTGGCACCCCAGAATTTGTGGC TCCTGAAGTGATCAACTATGAGCCCATCGGCTACGCCACAGACATGTGGAGCATCGGGGT CATCTGCTACATCCTAAACCGCCTGGACTGCACGCAGTGCCTTCAGCATCCATGGCTAAT GAAAGATACCAAGAACATGGAGGCCAAGAAACTCTCCAAGGACCGGATGAAGAAGTACAT GGCAAGAAGGAAATGGCAGAAAACGGGCAATGCTGTGAGAGCCATTGGAAGACTGTCCTC TATGGCAATGATCTCAGGGCTCAGTGGCAGGAAATCCTCAACAGGGTCACCAACCAGCCC GCTCAATGCAGAAAAACTAGAATCTGAAGAAGATGTGTCCCAAGCTTTCCTTGAGGCTGT TGCTGAGGAAAAGCCTCATGTAAAACCCTATTTCTCTAAGACCATTCGCGATTTAGAAGT TGTGGAGGGAAGTGCTGCTAGATTTGACTGCAAGATTGAAGGATACCCAGACCCCGAGGT TGTCTGGTTCAAAGATGACCAGTCAATCAGGGAGTCCCGCCACTTCCAGATAGACTACGA TGAGGACGGGAACTGCTCTTTAATTATTAGTGATGTTTGCGGGGATGACGATGCCAAGTA CACCTGCAAGGCTGTCAACAGTCTTGGAGAAGCCACCTGCACAGCAGAGCTCATTGTGGA AACGATGGAGGAAGGTGAAGGGGAAGGGGAAGAGGAAGAAGAGTGAAACAAAGCCAGAGA AAAGCAGTTTCTAAGTCATATTAAAAGGACTATTTCTCTAAAACTCAAAAAAAAAAAAAA AA >OriGene 5' read for NM_053028 unedited
AGTCAAAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGACCATGGGGGA TGTGAAGCTGGTTGCCTCGTCACACATTTCCAAAACCTCCCTCAGTGTGGATTCACTCAA GAGTTGACTCCATGCCCCTGACAGAGGCCCCTGCTTTCATTTTGCCCCCTCGGAACCTCT GCATCAAAGAAGGAGCCACCGCCAAGTTCGAAGGGCGGGTCCGGGGTTACCCAGAGCCCC AGGTGACATGGCACAGAAACGGGCAACCCATCACCAGCGGGGGCCGCTTCCTGCTGGATT GCGGCATCCGGGGGACTTTCAGCCTTGTGATTCATGCTGTCCATGAGGAGGACAGGGGAA AGTATACCTGTGAAGCCACCAATGGCAGTGGTGCTCGCCAGGTGACAGTGGAGTTGACAG TAGAAGGAAGTTTTGCGAAGCAGCTTGGTCAGCCTGTTGTTTCCAAAACCTTAGGGGATA GATTTTCAGCTCCAGCAGTGGAGACCCGTCCTAGCATCTGGGGGGAGTGCCCACCAAAGT TTGCTACCAAGCTGGGCCGAGTTGTGGTCAAAGAAGGACAGATGGGACGATTCTCCTGNC AGATCACTGGCCGGCCCCAACCGCANGTCACCTGGCTCAAGGGAAATGTTCCACTGCAGC CGAGTGCCCGTGTGTCTGTGTCTGAGAAGAACGCATGCANGTTCTGGAAATCCATGGAGT CAACCAAGATGACGTGGGGAGTGTACACGTGCCCTGNTGGTGAACGGNTCGGGGAAAGCC TCCATGTCAGCTGAACTTTCCATCCCAGGTTTGGACAGTGCCCATAGGTCATTTTGTGAG AAAAACAAAGCCACCAATCANATGTCAGNAAAAAGTGACCCATGTTATCTCAAAGAATCG AAGCTGGNCAGTCTTGNAAGCT >OriGene 3' read for NM_053028 unedited
CCCTTGGGATGGCACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGGATGCCAC CCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTT TTTTTTTTTTGAGTTTTAGAGAAATAGTCCTTTTAATATGACTTAGAAACTGCTTTTCTC TGGCTTTGTTTCACTCTTCTTCCTCTTCCCCTTCCCCTTCACCTTCCTCCATCGTTTCCA CAATGAGCTCTGCTGTGCAGGTGGCTTCTCCAAGACTGTTGACAGCCTTGCAGGTGTACT TGGCATCGTCATCCCCGCAAACATCACTAATAATTAAAGAGCAGTTCCCGTCCTCATCGT AGTCTATCTGGAAGTGGCGGGACTCCCTGATTGACTGGTCATCTTTGAACCAGACAACCT CGGGGTCTGGGTATCCTTCAATCTTGCAGTCAAATCTAGCAGCACTTCCCTCCACAACTT CTAAATCGCGAATGGTCTTAGAGAAATAGGGTTTTACATGAGGCTTTTCCTCAGCAACAG CCTCAAGGAAAGCTTGGGACACATCTTCTTCAGATTCTAGTTTTTCTGCATTGAGCGGGC TGGTTGGTGACCCTGTTGAGGATTTCCTGCCACTGAGCCCTGAGATCATTGCCATAGAGG ACAGTCTTCCAATGGCTCTCACAGCATTGCCCGTTTTCTGCCATTTCCTTCTTGCCATGT ACTTCTTCATCCGGTCCTTGGAGAGTTTCTTGGCCTCCATGTTCTTGGTATCTTTCATTA NCCATGGATGCTGAAGGCACTGCGTGCAGTCCCAGCGGTTTTAGATGTAGCANATGACCC CGATGCTCACATGTCTGTTGCGTACCCGATGGGCCTCATATTGATCCTTNCAGGAGCCAC AAATTCTGGGGTGCCAAGNAGGAC |
| Restriction Sites | Please inquire |
| ACCN | NM_053028 |
| Insert Size | 5500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | There is 5 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 1 aa and insertion of 5 aa. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_053028.2, NP_444256.2 |
| RefSeq Size | 7492 bp |
| RefSeq ORF | 5385 bp |
| Locus ID | 4638 |
| UniProt ID | Q15746 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Calcium signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Vascular smooth muscle contraction |
| Gene Summary | This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3B) does not utilize exons 11 and 30, compared to variant 1, resulting in a shorter protein (isoform 3B), compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
其它Myosin light chain kinase产品
Customer
Reviews
Loading...
