EIF4G1 (NM_198242) Human Untagged Clone

CAT#: SC309496

EIF4G1 (untagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 4



  "NM_198242" in other vectors (4)

CNY 10,450.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-EIF4G1 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18
Vector pCMV6 series
Sequence Data
>NCBI ORF sequence for NM_198242, the custom clone sequence may differ by one or more nucleotides
ATGAACCAGCCACCCCAGATTGCTCCCAAGAGGGAGCGTAAGACGATCCGAATTCGAGAT
CCAAACCAAGGAGGAAAGGATATCACAGAGGAGATCATGTCTGGGGCCCGCACTGCCTCC
ACACCCACCCCTCCCCAGACGGGAGGCGGTCTGGAGCCTCAAGCTAATGGGGAGACGCCC
CAGGTTGCTGTCATTGTCCGGCCAGATGACCGGTCACAGGGAGCAATCATTGCTGACCGG
CCAGGGCTGCCTGGCCCAGAGCATAGCCCTTCAGAATCCCAGCCTTCGTCGCCTTCTCCG
ACCCCATCACCATCCCCAGTCTTGGAACCGGGGTCTGAGCCTAATCTCGCAGTCCTCTCT
ATTCCTGGGGACACTATGACAACTATACAAATGTCTGTAGAAGAATCAACCCCCATCTCC
CGTGAAACTGGGGAGCCATATCGCCTCTCTCCAGAACCCACTCCTCTCGCCGAACCCATA
CTGGAAGTAGAAGTGACACTTAGCAAACCGGTTCCAGAATCTGAGTTTTCTTCCAGTCCT
CTCCAGGCTCCCACCCCTTTGGCATCTCACACAGTGGAAATTCATGAGCCTAATGGCATG
GTCCCATCTGAAGATCTGGAACCAGAGGTGGAGTCAAGCCCAGAGCTTGCTCCTCCCCCA
GCTTGCCCCTCCGAATCCCCTGTGCCCATTGCTCCAACTGCCCAACCTGAGGAACTGCTC
AACGGAGCCCCCTCGCCACCAGCTGTGGACTTAAGCCCAGTCAGTGAGCCAGAGGAGCAG
GCCAAGGAGGTGACAGCATCAATGGCGCCCCCCACCATCCCCTCTGCTACTCCAGCTACG
GCTCCTTCAGCTACTTCCCCAGCTCAGGAGGAGGAAATGGAAGAAGAAGAAGAAGAGGAA
GAAGGAGAAGCAGGAGAAGCAGGAGAAGCTGAGAGTGAGAAAGGAGGAGAGGAACTGCTC
CCCCCAGAGAGTACCCCTATTCCAGCCAACTTGTCTCAGAATTTGGAGGCAGCAGCAGCC
ACTCAAGTGGCAGTATCTGTGCCAAAGAGGAGACGGAAAATTAAGGAGCTAAATAAGAAG
GAGGCTGTTGGAGACCTTCTGGATGCCTTCAAGGAGGCGAACCCGGCAGTACCAGAGGTG
GAAAATCAGCCTCCTGCAGGCAGCAATCCAGGCCCAGAGTCTGAGGGCAGTGGTGTGCCC
CCACGTCCTGAGGAAGCAGATGAGACCTGGGACTCAAAGGAAGACAAAATTCACAATGCT
GAGAACATCCAGCCCGGGGAACAGAAGTATGAATATAAGTCAGATCAGTGGAAGCCTCTA
AACCTAGAGGAGAAAAAACGTTACGACCGTGAGTTCCTGCTTGGTTTTCAGTTCATCTTT
GCCAGTATGCAGAAGCCAGAGGGATTGCCACATATCAGTGACGTGGTGCTGGACAAGGCC
AATAAAACACCACTGCGGCCACTGGATCCCACTAGACTACAAGGCATAAATTGTGGCCCA
GACTTCACTCCATCCTTTGCCAACCTTGGCCGGACAACCCTTAGCACCCGTGGGCCCCCA
AGGGGTGGGCCAGGTGGGGAGCTGCCCCGTGGGCCGGCTGGCCTGGGACCCCGGCGCTCT
CAGCAGGGACCCCGAAAAGAACCACGCAAGATCATTGCCACAGTGTTAATGACCGAAGAT
ATAAAACTGAACAAAGCAGAGAAAGCCTGGAAACCCAGCAGCAAGCGGACGGCGGCTGAT
AAGGATCGAGGGGAAGAAGATGCTGATGGCAGCAAAACCCAGGACCTATTCCGCAGGGTG
CGCTCCATCCTGAATAAACTGACACCCCAGATGTTCCAGCAGCTGATGAAGCAAGTGACG
CAGCTGGCCATCGACACCGAGGAACGCCTCAAAGGGGTCATTGACCTCATTTTTGAGAAG
GCCATTTCAGAGCCCAACTTCTCTGTGGCCTATGCCAACATGTGCCGCTGCCTCATGGCG
CTGAAAGTGCCCACTACGGAAAAGCCAACAGTGACTGTGAACTTCCGAAAGCTGTTGTTG
AATCGATGTCAGAAGGAGTTTGAGAAAGACAAAGATGATGATGAGGTTTTTGAGAAGAAG
CAAAAAGAGATGGATGAAGCTGCTACGGCAGAGGAACGAGGACGCCTGAAGGAAGAGCTG
GAAGAGGCTCGGGACATAGCCCGGCGGCGCTCTTTAGGGAATATCAAGTTTATTGGAGAG
TTGTTCAAACTGAAGATGTTAACAGAGGCAATAATGCATGACTGTGTGGTCAAACTGCTT
AAGAACCATGATGAAGAGTCCCTTGAGTGCCTTTGTCGTCTGCTCACCACCATTGGCAAA
GACCTGGACTTTGAAAAAGCCAAGCCCCGAATGGATCAGTATTTCAACCAGATGGAAAAA
ATCATTAAAGAAAAGAAGACGTCATCCCGCATCCGCTTTATGCTGCAGGACGTGCTGGAT
CTGCGAGGGAGCAATTGGGTGCCACGCCGAGGGGATCAGGGTCCCAAGACCATTGACCAG
ATCCATAAGGAGGCTGAGATGGAAGAACATCGAGAGCACATCAAAGTGCAGCAGCTCATG
GCCAAGGGCAGTGACAAGCGTCGGGGCGGTCCTCCAGGCCCTCCCATCAGCCGTGGACTT
CCCCTTGTGGATGATGGTGGCTGGAACACAGTTCCCATCAGCAAAGGTAGCCGCCCCATT
GACACCTCACGACTCACCAAGATCACCAAGCCTGGCTCCATCGATTCTAACAACCAGCTC
TTTGCACCTGGAGGGCGACTGAGCTGGGGCAAGGGCAGCAGCGGAGGCTCAGGAGCCAAG
CCCTCAGACGCAGCATCAGAAGCTGCTCGCCCAGCTACTAGTACTTTGAATCGCTTCTCA
GCCCTTCAACAAGCGGTACCCACAGAAAGCACAGATAATAGACGTGTGGTGCAGAGGAGT
AGCTTGAGCCGAGAACGAGGCGAGAAAGCTGGAGACCGAGGAGACCGCCTAGAGCGGAGT
GAACGGGGAGGGGACCGTGGGGACCGGCTTGATCGTGCGCGGACACCTGCTACCAAGCGG
AGCTTCAGCAAGGAAGTGGAGGAGCGGAGTAGAGAACGGCCCTCCCAGCCTGAGGGGCTG
CGCAAGGCAGCTAGCCTCACGGAGGATCGGGACCGTGGGCGGGATGCCGTGAAGCGAGAA
GCTGCCCTACCCCCAGTGAGCCCCCTGAAGGCGGCTCTCTCTGAGGAGGAGTTAGAGAAG
AAATCCAAGGCTATCATTGAGGAATATCTCCATCTCAATGACATGAAAGAGGCAGTCCAG
TGCGTGCAGGAGCTGGCCTCACCCTCCTTGCTCTTCATCTTTGTACGGCATGGTGTCGAG
TCTACGCTGGAGCGCAGTGCCATTGCTCGTGAGCATATGGGGCAGCTGCTGCACCAGCTG
CTCTGTGCTGGGCATCTGTCTACTGCTCAGTACTACCAAGGGTTGTATGAAATCTTGGAA
TTGGCTGAGGACATGGAAATTGACATCCCCCACGTGTGGCTCTACCTAGCGGAACTGGTA
ACACCCATTCTGCAGGAAGGTGGGGTGCCCATGGGGGAGCTGTTCAGGGAGATTACAAAG
CCTCTGAGACCGTTGGGCAAAGCTGCTTCCCTGTTGCTGGAGATCCTGGGCCTCCTGTGC
AAAAGCATGGGTCCTAAAAAGGTGGGGACGCTGTGGCGAGAAGCCGGGCTTAGCTGGAAG
GAATTTCTACCTGAAGGCCAGGACATTGGTGCATTCGTCGCTGAACAGAAGGTGGAGTAT
ACCCTGGGAGAGGAGTCGGAAGCCCCTGGCCAGAGGGCACTCCCCTCCGAGGAGCTGAAC
AGGCAGCTGGAGAAGCTGCTGAAGGAGGGCAGCAGTAACCAGCGGGTGTTCGACTGGATA
GAGGCCAACCTGAGTGAGCAGCAGATAGTATCCAACACGTTAGTTCGAGCCCTCATGACG
GCTGTCTGCTATTCTGCAATTATTTTTGAGACTCCCCTCCGAGTGGACGTTGCAGTGCTG
AAAGCGCGAGCGAAGCTGCTGCAGAAATACCTGTGTGACGAGCAGAAGGAGCTACAGGCG
CTCTACGCCCTCCAGGCCCTTGTAGTGACCTTAGAACAGCCTCCCAACCTGCTGCGGATG
TTCTTTGACGCACTGTATGACGAGGACGTGGTGAAGGAGGATGCCTTCTACAGTTGGGAG
AGTAGCAAGGACCCCGCTGAGCAGCAGGGCAAGGGTGTGGCCCTTAAATCTGTCACAGCC
TTCTTCAAGTGGCTCCGTGAAGCAGAGGAGGAGTCTGACCACAACTGA
Restriction Sites Please inquire     
ACCN NM_198242
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_198242.1, NP_937885.1
RefSeq Size 5048 bp
RefSeq ORF 4308 bp
Locus ID 1981
UniProt ID Q04637
Protein Pathways Viral myocarditis
Gene Summary The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010]
Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, uses a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (3) is shorter at the N-terminus and lacks an internal residue, compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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