EIF4G1 (NM_198244) Human Untagged Clone
CAT#: SC309370
EIF4G1 (untagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 3
CNY 10,450.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18 |
Vector | pCMV6 series |
Sequence Data |
>NCBI ORF sequence for NM_198244, the custom clone sequence may differ by one or more nucleotides
ATGATGATCCCTTCCCAGATCTCCTACCCAGCCTCCCAGGGGGCCTACTACATCCCTGGA CAGGGGCGTTCCACATACGTTGTCCCGACACAGCAGTACCCTGTGCAGCCAGGAGCCCCA GGCTTCTATCCAGGTGCAAGCCCTACAGAATTTGGGACCTACGCTGGCGCCTACTATCCA GCCCAAGGGGTGCAGCAGTTTCCCACTGGCGTGGCCCCCGCCCCAGTTTTGATGAACCAG CCACCCCAGATTGCTCCCAAGAGGGAGCGTAAGACGATCCGAATTCGAGATCCAAACCAA GGAGGAAAGGATATCACAGAGGAGATCATGTCTGGGGCCCGCACTGCCTCCACACCCACC CCTCCCCAGACGGGAGGCGGTCTGGAGCCTCAAGCTAATGGGGAGACGCCCCAGGTTGCT GTCATTGTCCGGCCAGATGACCGGTCACAGGGAGCAATCATTGCTGACCGGCCAGGGCTG CCTGGCCCAGAGCATAGCCCTTCAGAATCCCAGCCTTCGTCGCCTTCTCCGACCCCATCA CCATCCCCAGTCTTGGAACCGGGGTCTGAGCCTAATCTCGCAGTCCTCTCTATTCCTGGG GACACTATGACAACTATACAAATGTCTGTAGAAGAATCAACCCCCATCTCCCGTGAAACT GGGGAGCCATATCGCCTCTCTCCAGAACCCACTCCTCTCGCCGAACCCATACTGGAAGTA GAAGTGACACTTAGCAAACCGGTTCCAGAATCTGAGTTTTCTTCCAGTCCTCTCCAGGCT CCCACCCCTTTGGCATCTCACACAGTGGAAATTCATGAGCCTAATGGCATGGTCCCATCT GAAGATCTGGAACCAGAGGTGGAGTCAAGCCCAGAGCTTGCTCCTCCCCCAGCTTGCCCC TCCGAATCCCCTGTGCCCATTGCTCCAACTGCCCAACCTGAGGAACTGCTCAACGGAGCC CCCTCGCCACCAGCTGTGGACTTAAGCCCAGTCAGTGAGCCAGAGGAGCAGGCCAAGGAG GTGACAGCATCAATGGCGCCCCCCACCATCCCCTCTGCTACTCCAGCTACGGCTCCTTCA GCTACTTCCCCAGCTCAGGAGGAGGAAATGGAAGAAGAAGAAGAAGAGGAAGAAGGAGAA GCAGGAGAAGCAGGAGAAGCTGAGAGTGAGAAAGGAGGAGAGGAACTGCTCCCCCCAGAG AGTACCCCTATTCCAGCCAACTTGTCTCAGAATTTGGAGGCAGCAGCAGCCACTCAAGTG GCAGTATCTGTGCCAAAGAGGAGACGGAAAATTAAGGAGCTAAATAAGAAGGAGGCTGTT GGAGACCTTCTGGATGCCTTCAAGGAGGCGAACCCGGCAGTACCAGAGGTGGAAAATCAG CCTCCTGCAGGCAGCAATCCAGGCCCAGAGTCTGAGGGCAGTGGTGTGCCCCCACGTCCT GAGGAAGCAGATGAGACCTGGGACTCAAAGGAAGACAAAATTCACAATGCTGAGAACATC CAGCCCGGGGAACAGAAGTATGAATATAAGTCAGATCAGTGGAAGCCTCTAAACCTAGAG GAGAAAAAACGTTACGACCGTGAGTTCCTGCTTGGTTTTCAGTTCATCTTTGCCAGTATG CAGAAGCCAGAGGGATTGCCACATATCAGTGACGTGGTGCTGGACAAGGCCAATAAAACA CCACTGCGGCCACTGGATCCCACTAGACTACAAGGCATAAATTGTGGCCCAGACTTCACT CCATCCTTTGCCAACCTTGGCCGGACAACCCTTAGCACCCGTGGGCCCCCAAGGGGTGGG CCAGGTGGGGAGCTGCCCCGTGGGCCGGCTGGCCTGGGACCCCGGCGCTCTCAGCAGGGA CCCCGAAAAGAACCACGCAAGATCATTGCCACAGTGTTAATGACCGAAGATATAAAACTG AACAAAGCAGAGAAAGCCTGGAAACCCAGCAGCAAGCGGACGGCGGCTGATAAGGATCGA GGGGAAGAAGATGCTGATGGCAGCAAAACCCAGGACCTATTCCGCAGGGTGCGCTCCATC CTGAATAAACTGACACCCCAGATGTTCCAGCAGCTGATGAAGCAAGTGACGCAGCTGGCC ATCGACACCGAGGAACGCCTCAAAGGGGTCATTGACCTCATTTTTGAGAAGGCCATTTCA GAGCCCAACTTCTCTGTGGCCTATGCCAACATGTGCCGCTGCCTCATGGCGCTGAAAGTG CCCACTACGGAAAAGCCAACAGTGACTGTGAACTTCCGAAAGCTGTTGTTGAATCGATGT CAGAAGGAGTTTGAGAAAGACAAAGATGATGATGAGGTTTTTGAGAAGAAGCAAAAAGAG ATGGATGAAGCTGCTACGGCAGAGGAACGAGGACGCCTGAAGGAAGAGCTGGAAGAGGCT CGGGACATAGCCCGGCGGCGCTCTTTAGGGAATATCAAGTTTATTGGAGAGTTGTTCAAA CTGAAGATGTTAACAGAGGCAATAATGCATGACTGTGTGGTCAAACTGCTTAAGAACCAT GATGAAGAGTCCCTTGAGTGCCTTTGTCGTCTGCTCACCACCATTGGCAAAGACCTGGAC TTTGAAAAAGCCAAGCCCCGAATGGATCAGTATTTCAACCAGATGGAAAAAATCATTAAA GAAAAGAAGACGTCATCCCGCATCCGCTTTATGCTGCAGGACGTGCTGGATCTGCGAGGG AGCAATTGGGTGCCACGCCGAGGGGATCAGGGTCCCAAGACCATTGACCAGATCCATAAG GAGGCTGAGATGGAAGAACATCGAGAGCACATCAAAGTGCAGCAGCTCATGGCCAAGGGC AGTGACAAGCGTCGGGGCGGTCCTCCAGGCCCTCCCATCAGCCGTGGACTTCCCCTTGTG GATGATGGTGGCTGGAACACAGTTCCCATCAGCAAAGGTAGCCGCCCCATTGACACCTCA CGACTCACCAAGATCACCAAGCCTGGCTCCATCGATTCTAACAACCAGCTCTTTGCACCT GGAGGGCGACTGAGCTGGGGCAAGGGCAGCAGCGGAGGCTCAGGAGCCAAGCCCTCAGAC GCAGCATCAGAAGCTGCTCGCCCAGCTACTAGTACTTTGAATCGCTTCTCAGCCCTTCAA CAAGCGGTACCCACAGAAAGCACAGATAATAGACGTGTGGTGCAGAGGAGTAGCTTGAGC CGAGAACGAGGCGAGAAAGCTGGAGACCGAGGAGACCGCCTAGAGCGGAGTGAACGGGGA GGGGACCGTGGGGACCGGCTTGATCGTGCGCGGACACCTGCTACCAAGCGGAGCTTCAGC AAGGAAGTGGAGGAGCGGAGTAGAGAACGGCCCTCCCAGCCTGAGGGGCTGCGCAAGGCA GCTAGCCTCACGGAGGATCGGGACCGTGGGCGGGATGCCGTGAAGCGAGAAGCTGCCCTA CCCCCAGTGAGCCCCCTGAAGGCGGCTCTCTCTGAGGAGGAGTTAGAGAAGAAATCCAAG GCTATCATTGAGGAATATCTCCATCTCAATGACATGAAAGAGGCAGTCCAGTGCGTGCAG GAGCTGGCCTCACCCTCCTTGCTCTTCATCTTTGTACGGCATGGTGTCGAGTCTACGCTG GAGCGCAGTGCCATTGCTCGTGAGCATATGGGGCAGCTGCTGCACCAGCTGCTCTGTGCT GGGCATCTGTCTACTGCTCAGTACTACCAAGGGTTGTATGAAATCTTGGAATTGGCTGAG GACATGGAAATTGACATCCCCCACGTGTGGCTCTACCTAGCGGAACTGGTAACACCCATT CTGCAGGAAGGTGGGGTGCCCATGGGGGAGCTGTTCAGGGAGATTACAAAGCCTCTGAGA CCGTTGGGCAAAGCTGCTTCCCTGTTGCTGGAGATCCTGGGCCTCCTGTGCAAAAGCATG GGTCCTAAAAAGGTGGGGACGCTGTGGCGAGAAGCCGGGCTTAGCTGGAAGGAATTTCTA CCTGAAGGCCAGGACATTGGTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGA GAGGAGTCGGAAGCCCCTGGCCAGAGGGCACTCCCCTCCGAGGAGCTGAACAGGCAGCTG GAGAAGCTGCTGAAGGAGGGCAGCAGTAACCAGCGGGTGTTCGACTGGATAGAGGCCAAC CTGAGTGAGCAGCAGATAGTATCCAACACGTTAGTTCGAGCCCTCATGACGGCTGTCTGC TATTCTGCAATTATTTTTGAGACTCCCCTCCGAGTGGACGTTGCAGTGCTGAAAGCGCGA GCGAAGCTGCTGCAGAAATACCTGTGTGACGAGCAGAAGGAGCTACAGGCGCTCTACGCC CTCCAGGCCCTTGTAGTGACCTTAGAACAGCCTCCCAACCTGCTGCGGATGTTCTTTGAC GCACTGTATGACGAGGACGTGGTGAAGGAGGATGCCTTCTACAGTTGGGAGAGTAGCAAG GACCCCGCTGAGCAGCAGGGCAAGGGTGTGGCCCTTAAATCTGTCACAGCCTTCTTCAAG TGGCTCCGTGAAGCAGAGGAGGAGTCTGACCACAACTGA |
Restriction Sites | Please inquire |
ACCN | NM_198244 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_198244.1, NP_937887.1 |
RefSeq Size | 5225 bp |
RefSeq ORF | 4539 bp |
Locus ID | 1981 |
Protein Pathways | Viral myocarditis |
Gene Summary | The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, uses a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (2) is shorter at the N-terminus and lacks an internal residue, compared to isoform 1. |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219966 | EIF4G1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 3 |
CNY 15,110.00 |
|
RC219966L3 | Lenti-ORF clone of EIF4G1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 3 |
CNY 17,010.00 |
|
RC219966L4 | Lenti-ORF clone of EIF4G1 (mGFP-tagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 3 |
CNY 17,010.00 |
|
RG219966 | EIF4G1 (tGFP-tagged) - Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 3 |
CNY 16,630.00 |