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EIF4G1 (NM_198241) Human Untagged Clone
CAT＃: SC309369
EIF4G1 (untagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 2
CNY 30,780.00
货期*
4周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC309369 representing NM_198241.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAACAAAGCTCCACAGTCCACAGGCCCCCCACCCGCCCCATCCCCCGGACTCCCACAGCCAGCGTTT CCCCCGGGGCAGACAGCGCCGGTGGTGTTCAGTACGCCACAAGCGACACAAATGAACACGCCTTCTCAG CCCCGCCAGCACTTCTACCCTAGCCGGGCCCAGCCCCCGAGCAGTGCAGCCTCCCGAGTGCAGAGTGCA GCCCCTGCCCGCCCTGGCCCAGCTGCCCATGTCTACCCTGCTGGATCCCAAGTAATGATGATCCCTTCC CAGATCTCCTACCCAGCCTCCCAGGGGGCCTACTACATCCCTGGACAGGGGCGTTCCACATACGTTGTC CCGACACAGCAGTACCCTGTGCAGCCAGGAGCCCCAGGCTTCTATCCAGGTGCAAGCCCTACAGAATTT GGGACCTACGCTGGCGCCTACTATCCAGCCCAAGGGGTGCAGCAGTTTCCCACTGGCGTGGCCCCCGCC CCAGTTTTGATGAACCAGCCACCCCAGATTGCTCCCAAGAGGGAGCGTAAGACGATCCGAATTCGAGAT CCAAACCAAGGAGGAAAGGATATCACAGAGGAGATCATGTCTGGGGCCCGCACTGCCTCCACACCCACC CCTCCCCAGACGGGAGGCGGTCTGGAGCCTCAAGCTAATGGGGAGACGCCCCAGGTTGCTGTCATTGTC CGGCCAGATGACCGGTCACAGGGAGCAATCATTGCTGACCGGCCAGGGCTGCCTGGCCCAGAGCATAGC CCTTCAGAATCCCAGCCTTCGTCGCCTTCTCCGACCCCATCACCATCCCCAGTCTTGGAACCGGGGTCT GAGCCTAATCTCGCAGTCCTCTCTATTCCTGGGGACACTATGACAACTATACAAATGTCTGTAGAAGAA TCAACCCCCATCTCCCGTGAAACTGGGGAGCCATATCGCCTCTCTCCAGAACCCACTCCTCTCGCCGAA CCCATACTGGAAGTAGAAGTGACACTTAGCAAACCGGTTCCAGAATCTGAGTTTTCTTCCAGTCCTCTC CAGGCTCCCACCCCTTTGGCATCTCACACAGTGGAAATTCATGAGCCTAATGGCATGGTCCCATCTGAA GATCTGGAACCAGAGGTGGAGTCAAGCCCAGAGCTTGCTCCTCCCCCAGCTTGCCCCTCCGAATCCCCT GTGCCCATTGCTCCAACTGCCCAACCTGAGGAACTGCTCAACGGAGCCCCCTCGCCACCAGCTGTGGAC TTAAGCCCAGTCAGTGAGCCAGAGGAGCAGGCCAAGGAGGTGACAGCATCAATGGCGCCCCCCACCATC CCCTCTGCTACTCCAGCTACGGCTCCTTCAGCTACTTCCCCAGCTCAGGAGGAGGAAATGGAAGAAGAA GAAGAAGAGGAAGAAGGAGAAGCAGGAGAAGCAGGAGAAGCTGAGAGTGAGAAAGGAGGAGAGGAACTG CTCCCCCCAGAGAGTACCCCTATTCCAGCCAACTTGTCTCAGAATTTGGAGGCAGCAGCAGCCACTCAA GTGGCAGTATCTGTGCCAAAGAGGAGACGGAAAATTAAGGAGCTAAATAAGAAGGAGGCTGTTGGAGAC CTTCTGGATGCCTTCAAGGAGGCGAACCCGGCAGTACCAGAGGTGGAAAATCAGCCTCCTGCAGGCAGC AATCCAGGCCCAGAGTCTGAGGGCAGTGGTGTGCCCCCACGTCCTGAGGAAGCAGATGAGACCTGGGAC TCAAAGGAAGACAAAATTCACAATGCTGAGAACATCCAGCCCGGGGAACAGAAGTATGAATATAAGTCA GATCAGTGGAAGCCTCTAAACCTAGAGGAGAAAAAACGTTACGACCGTGAGTTCCTGCTTGGTTTTCAG TTCATCTTTGCCAGTATGCAGAAGCCAGAGGGATTGCCACATATCAGTGACGTGGTGCTGGACAAGGCC AATAAAACACCACTGCGGCCACTGGATCCCACTAGACTACAAGGCATAAATTGTGGCCCAGACTTCACT CCATCCTTTGCCAACCTTGGCCGGACAACCCTTAGCACCCGTGGGCCCCCAAGGGGTGGGCCAGGTGGG GAGCTGCCCCGTGGGCCGGCTGGCCTGGGACCCCGGCGCTCTCAGCAGGGACCCCGAAAAGAACCACGC AAGATCATTGCCACAGTGTTAATGACCGAAGATATAAAACTGAACAAAGCAGAGAAAGCCTGGAAACCC AGCAGCAAGCGGACGGCGGCTGATAAGGATCGAGGGGAAGAAGATGCTGATGGCAGCAAAACCCAGGAC CTATTCCGCAGGGTGCGCTCCATCCTGAATAAACTGACACCCCAGATGTTCCAGCAGCTGATGAAGCAA GTGACGCAGCTGGCCATCGACACCGAGGAACGCCTCAAAGGGGTCATTGACCTCATTTTTGAGAAGGCC ATTTCAGAGCCCAACTTCTCTGTGGCCTATGCCAACATGTGCCGCTGCCTCATGGCGCTGAAAGTGCCC ACTACGGAAAAGCCAACAGTGACTGTGAACTTCCGAAAGCTGTTGTTGAATCGATGTCAGAAGGAGTTT GAGAAAGACAAAGATGATGATGAGGTTTTTGAGAAGAAGCAAAAAGAGATGGATGAAGCTGCTACGGCA GAGGAACGAGGACGCCTGAAGGAAGAGCTGGAAGAGGCTCGGGACATAGCCCGGCGGCGCTCTTTAGGG AATATCAAGTTTATTGGAGAGTTGTTCAAACTGAAGATGTTAACAGAGGCAATAATGCATGACTGTGTG GTCAAACTGCTTAAGAACCATGATGAAGAGTCCCTTGAGTGCCTTTGTCGTCTGCTCACCACCATTGGC AAAGACCTGGACTTTGAAAAAGCCAAGCCCCGAATGGATCAGTATTTCAACCAGATGGAAAAAATCATT AAAGAAAAGAAGACGTCATCCCGCATCCGCTTTATGCTGCAGGACGTGCTGGATCTGCGAGGGAGCAAT TGGGTGCCACGCCGAGGGGATCAGGGTCCCAAGACCATTGACCAGATCCATAAGGAGGCTGAGATGGAA GAACATCGAGAGCACATCAAAGTGCAGCAGCTCATGGCCAAGGGCAGTGACAAGCGTCGGGGCGGTCCT CCAGGCCCTCCCATCAGCCGTGGACTTCCCCTTGTGGATGATGGTGGCTGGAACACAGTTCCCATCAGC AAAGGTAGCCGCCCCATTGACACCTCACGACTCACCAAGATCACCAAGCCTGGCTCCATCGATTCTAAC AACCAGCTCTTTGCACCTGGAGGGCGACTGAGCTGGGGCAAGGGCAGCAGCGGAGGCTCAGGAGCCAAG CCCTCAGACGCAGCATCAGAAGCTGCTCGCCCAGCTACTAGTACTTTGAATCGCTTCTCAGCCCTTCAA CAAGCGGTACCCACAGAAAGCACAGATAATAGACGTGTGGTGCAGAGGAGTAGCTTGAGCCGAGAACGA GGCGAGAAAGCTGGAGACCGAGGAGACCGCCTAGAGCGGAGTGAACGGGGAGGGGACCGTGGGGACCGG CTTGATCGTGCGCGGACACCTGCTACCAAGCGGAGCTTCAGCAAGGAAGTGGAGGAGCGGAGTAGAGAA CGGCCCTCCCAGCCTGAGGGGCTGCGCAAGGCAGCTAGCCTCACGGAGGATCGGGACCGTGGGCGGGAT GCCGTGAAGCGAGAAGCTGCCCTACCCCCAGTGAGCCCCCTGAAGGCGGCTCTCTCTGAGGAGGAGTTA GAGAAGAAATCCAAGGCTATCATTGAGGAATATCTCCATCTCAATGACATGAAAGAGGCAGTCCAGTGC GTGCAGGAGCTGGCCTCACCCTCCTTGCTCTTCATCTTTGTACGGCATGGTGTCGAGTCTACGCTGGAG CGCAGTGCCATTGCTCGTGAGCATATGGGGCAGCTGCTGCACCAGCTGCTCTGTGCTGGGCATCTGTCT ACTGCTCAGTACTACCAAGGGTTGTATGAAATCTTGGAATTGGCTGAGGACATGGAAATTGACATCCCC CACGTGTGGCTCTACCTAGCGGAACTGGTAACACCCATTCTGCAGGAAGGTGGGGTGCCCATGGGGGAG CTGTTCAGGGAGATTACAAAGCCTCTGAGACCGTTGGGCAAAGCTGCTTCCCTGTTGCTGGAGATCCTG GGCCTCCTGTGCAAAAGCATGGGTCCTAAAAAGGTGGGGACGCTGTGGCGAGAAGCCGGGCTTAGCTGG AAGGAATTTCTACCTGAAGGCCAGGACATTGGTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTG GGAGAGGAGTCGGAAGCCCCTGGCCAGAGGGCACTCCCCTCCGAGGAGCTGAACAGGCAGCTGGAGAAG CTGCTGAAGGAGGGCAGCAGTAACCAGCGGGTGTTCGACTGGATAGAGGCCAACCTGAGTGAGCAGCAG ATAGTATCCAACACGTTAGTTCGAGCCCTCATGACGGCTGTCTGCTATTCTGCAATTATTTTTGAGACT CCCCTCCGAGTGGACGTTGCAGTGCTGAAAGCGCGAGCGAAGCTGCTGCAGAAATACCTGTGTGACGAG CAGAAGGAGCTACAGGCGCTCTACGCCCTCCAGGCCCTTGTAGTGACCTTAGAACAGCCTCCCAACCTG CTGCGGATGTTCTTTGACGCACTGTATGACGAGGACGTGGTGAAGGAGGATGCCTTCTACAGTTGGGAG AGTAGCAAGGACCCCGCTGAGCAGCAGGGCAAGGGTGTGGCCCTTAAATCTGTCACAGCCTTCTTCAAG TGGCTCCGTGAAGCAGAGGAGGAGTCTGACCACAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_198241 |
| Insert Size | 4800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_198241.2 |
| RefSeq Size | 5538 bp |
| RefSeq ORF | 4800 bp |
| Locus ID | 1981 |
| UniProt ID | Q04637 |
| Protein Pathways | Viral myocarditis |
| MW | 175.5 kDa |
| Gene Summary | The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010] Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (5) that is 1 aa shorter than isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219841 | EIF4G1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 2 |
CNY 10,944.00 |
|
| RC219841L3 | Lenti-ORF clone of EIF4G1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 2 |
CNY 15,680.00 |
|
| RC219841L4 | Lenti-ORF clone of EIF4G1 (mGFP-tagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 2 |
CNY 15,680.00 |
|
| RG219841 | EIF4G1 (tGFP-tagged) - Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 2 |
CNY 15,200.00 |
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