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Myosin light chain kinase (MYLK) (NM_053029) Human Untagged Clone
CAT＃: SC309074
MYLK (untagged)-Human myosin, light polypeptide kinase (MYLK), transcript variant 4
CNY 16,792.00
货期*
现货
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KRP, MLCK, MLCK108, MLCK210, MSTP083, FLJ12216, DKFZp686I10125 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_053029 edited
ACCATGGGGGATGTGAAGCTGGTTGCCTCGTCACACATTTCCAAAACCTCCCTCAGTGTG GATCACTCAAGAGTTGACTCCATGCCCCTGACAGAGGCCCCTGCTTTCATTTTGCCCCCT CGGAACCTCTGCATCAAAGAAGGAGCCACCGCCAAGTTCGAAGGGCGGGTCCGGGGTTAC CCAGAGCCCCAGGTGACATGGCACAGAAACGGGCAACCCATCACCAGCGGGGGCCGCTTC CTGCTGGATTGCGGCATCCGGGGGACTTTCAGCCTTGTGATTCATGCTGTCCATGAGGAG GACAGGGGAAAGTATACCTGTGAAGCCACCAATGGCAGTGGTGCTCGCCAGGTGACAGTG GAGTTGACAGTAGAAGGAAGTTTTGCGAAGCAGCTTGGTCAGCCTGTTGTTTCCAAAACC TTAGGGGATAGATTTTCAGCTCCAGCAGTGGAGACCCGTCCTAGCATCTGGGGGGAGTGC CCACCAAAGTTTGCTACCAAGCTGGGCCGAGTTGTGGTCAAAGAAGGACAGATGGGACGA TTCTCCTGCAAGATCACTGGCCGGCCCCAACCGCAGGTCACCTGGCTCAAGGGAAATGTT CCACTGCAGCCGAGTGCCCGTGTGTCTGTGTCTGAGAAGAACGGCATGCAGGTTCTGGAA ATCCATGGAGTCAACCAAGATGACGTGGGAGTGTACACGTGCCTGGTGGTGAACGGGTCG GGGAAGGCCTCGATGTCAGCTGAACTTTCCATCCAAGGTTTGGACAGTGCCAATAGGTCA TTTGTGAGAGAAACAAAAGCCACCAATTCAGATGTCAGGAAAGAGGTGACCAATGTAATC TCAAAGGAGTCGAAGCTGGACAGTCTGGAGGCTGCAGCCAAAAGCAAGAACTGCTCCAGC CCCCAGAGAGGTGGCTCCCCACCCTGGGCTGCAAACAGCCAGCCTCAGCCCCCAAGGGAG TCCAAGCTGGAGTCATGCAAGGACTCGCCCAGAACGGCCCCGCAGACCCCGGTCCTTCAG AAGACTTCCAGCTCCATCACCCTGCAGGCCGCAAGAGTTCAGCCGGAACCAAGAGCACCA GGCCTGGGGGTCCTATCACCTTCTGGAGAAGAGAGGAAGAGGCCAGCTCCTCCCCGTCCA GCCACCTTCCCCACCAGGCAGCCTGGCCTGGGGAGCCAAGATGTTGTGAGCAAGGCTGCT AACAGGAGAATCCCCATGGAGGGCCAGAGGGATTCAGCATTCCCCAAATTTGAGAGCAAG CCCCAAAGCCAGGAGGTCAAGGAAAATCAAACTGTCAAGTTCAGATGTGAAGTTTCCGGG ATTCCAAAGCCTGAAGTGGCCTGGTTCCTGGAAGGCACCCCCGTGAGGAGACAGGAAGGC AGCATTGAGGTTTATGAAGATGCTGGCTCCCATTACCTCTGCCTGCTGAAAGCCCGGACC AGGGACAGTGGGACATACAGCTGCACTGCTTCCAACGCCCAAGGCCAGGTGTCCTGTAGC TGGACCCTCCAAGTGGAAAGGCTTGCCGTGATGGAGGTGGCCCCCTCCTTCTCCAGTGTC CTGAAGGACTGCGCTGTTATTGAGGGCCAGGATTTTGTGCTGCAGTGCTCCGTACGGGGG ACCCCAGTGCCCCGGATCACTTGGCTGCTGAATGGGCAGCCCATCCAGTACGCTCGCTCC ACCTGCGAGGCCGGCGTGGCTGAGCTCCACATCCAGGATGCCCTGCCGGAGGACCATGGC ACCTACACCTGCCTAGCTGAGAATGCCTTGGGGCAGGTGTCCTGCAGCGCCTGGGTCACC GTCCATGAAAAGAAGAGTAGCAGGAAGAGTGAGTACCTTCTGCCTGTGGCTCCCAGCAAG CCCACTGCACCCATCTTCCTGCAGGGCCTCTCTGATCTCAAAGTCATGGATGGAAGCCAG GTCACTATGACTGTCCAAGTGTCAGGGAATCCACCCCCTGAAGTCATCTGGCTGCACAAT GGGAATGAGATCCAAGAGTCAGAGGACTTCCACTTTGAACAGAGAGGAACTCAGCACAGC CTTTGTATCCAGGAAGTGTTCCCGGAGGACACGGGCACGTACACCTGCGAGGCCTGGAAC AGCGCTGGAGAGGTCCGCACCCAGGCCGTGCTCACGGTACAAGAGCCTCACGATGGCACC CAGCCCTGGTTCATCAGTAAGCCTCGCTCAGTGACAGCCTCCCTGGGCCAGAGTGTCCTC ATCTCCTGCGCCATAGCTGGTGACCCCTTTCCTACCGTGCACTGGCTCAGAGATGGCAAA GCCCTCTGCAAAGACACTGGCCACTTCGAGGTGCTTCAGAATGAGGACGTGTTCACCCTG GTTCTAAAGAAGGTGCAGCCCTGGCATGCCGGCCAGTATGAGATCCTGCTCAAGAACCGG GTTGGCGAATGCAGTTGCCAGGTGTCACTGATGCTACAGAACAGCTCTGCCAGAGCCCTT CCACGGGGGAGGGAGCCTGCCAGCTGCGAGGACCTCTGTGGTGGAGGAGTTGGTGCTGAT GGTGGTGGTAGTGACTGCTATGGGTCCCTGAGGCCTGGCTGGCCAGCAAGAGGGCAGGGT TGGCCAGAGGAGGAAGACGGCGAGGACGTGCGAGGGGTGCTGAAGAGGCGCGTGGAGACG AGGCAGCACACTGAGGAGGCGATCCGCCAGCAGGAGGTGGAGCAGCTGGACTTCCGAGAC CTCCTGGGGAAGAAGGTGAGTACAAAGACCCTATCGGAAGACGAACTGAAGGAGATCCCA GCCGAGCAGATGGATTTCCGTGCCAACCTGCAGCGGCAAGTGAAGCCAAAGACTGTGTCT GAGGAAGAGAGGAAGGTGCACAGCCCCCAGCAGGTCGATTTTCGCTCTGTCCTGGCCAAG AAGGGGACTTCCAAGACCCCCGTGCCTGAGAAGGTGCCACCGCCAAAACCTGCCACCCCG GATTTTCGCTCAGTGCTGGGTGGCAAGAAGAAATTACCAGCAGAGAATGGCAGCAGCAGT GCCGAGACCCTGAATGCCAAGGCAGTGGAGAGTTCCAAGCCCCTGAGCAATGCACAGCCT TCAGGGCCCTTGAAACCCGTGGGCAACGCCAAGCCTGCTGAGACCCTGAAGCCAATGGGC AACGCCAAGCCTGCCGAGACCCTGAAGCCCATGGGCAATGCCAAGCCTGATGAGAACCTG AAATCCGCTAGCAAAGAAGAACTCAAGAAAGACGTTAAGAATGATGTGAACTGCAAGAGA GGCCATGCAGGGACCACAGATAATGAAAAGAGATCAGAGAGCCAGGGGACAGCCCCAGCC TTCAAGCAGAAGCTGCAAGATGTTCATGTGGCAGAGGGCAAGAAGCTGCTGCTCCAGTGC CAGGTGTCTTCTGACCCCCCAGCCACCATCATCTGGACGCTGAATGGAAAGACCCTCAAG ACCACCAAGTTCATCATCCTCTCCCAGGAAGGCTCACTCTGCTCCGTCTCCATCGAGAAG GCACTGCCTGAGGACAGAGGCTTATACAAGTGTGTAGCCAAGAATGACGCTGGCCAGGCG GAGTGCTCCTGCCAAGTCACCGTGGATGATGCTCCAGCCAGTGAGAACACCAAGGCCCCA GAGATGAAATCCCGGAGGCCCAAGAGCTCTCTTCCTCCCGTGCTAGGAACTGAGAGTGAT GCGACTGTGAAAAAGAAACCTGCCCCCAAGACACCTCCGAAGGCAGCAATGCCCCCTCAG ATCATCCAGTTCCCTGAGGACCAGAAGGTACGCGCAGGAGAGTCAGTGGAGCTGTTTGGC AAAGTGACAGGCACTCAGCCCATCACCTGTACCTGGATGAAGTTCCGAAAGCAGATCCAG GAAAGCGAGCACATGAAGGTGGAGAACAGCGAGAATGGCAGCAAGCTCACCATCCTGGCC GCGCGCCAGGAGCACTGCGGCTGCTACACACTGCTGGTGGAGAACAAGCTGGGCAGCAGG CAGGCCCAGGTCAACCTCACTGTCGTGGATAAGCCAGACCCCCCAGCTGGCACACCTTGT GCCTCTGACATTCGGAGCTCCTCACTGACCCTGTCCTGGTATGGCTCCTCATATGATGGG GGCAGTGCTGTACAGTCCTACAGCATCGAGATCTGGGACTCAGCCAACAAGACGTGGAAG GAACTAGCCACATGCCGCAGCACCTCTTTCAACGTCCAGGACCTGCTGCCTGACCACGAA TATAAGTTCCGTGTACGTGCAATCAACGTGTATGGAACCAGTGAGCCAAGCCAGGAGTCT GAACTCACAACGGTAGGAGAGAAACCTGAAGAGCCGAAGGATGAAGTGGAGGTGTCAGAC GATGATGAGAAGGAGCCCGAGGTTGATTACCGGACAGTGACAATCAATACTGAACAAAAA GTATCTGACTTCTACGACATTGAGGAGAGATTAGGATCCTGTTTGAGCGCATCATTGACG AGGACTTTGAGCTGACGGAGCGTGAGTGCATCAAGTACATGCGGCAGATCTCGGAGGGAG TGGAGTACATCCACAAGCAGGGCATCGTGCACCTGGACCTCAAGCCGGAGAACATCATGT GTGTCAACAAGACGGGCACCAGGATCAAGCTCATCGACTTTGGTCTGGCCAGGAGGCTGG AGAACGCGGGGTCTCTGAAGGTCCTCTTTGGCACCCCAGAATTTGTGGCTCCTGAAGTGA TCAACTATGAGCCCATCGGCTACGCCACAGACATGTGGAGCATCGGGGTCATCTGCTACA TCCTAGTCAGTGGCCTTTCCCCCTTCATGGGAGACAACGATAACGAAACCTTGGCCAACG TTACCTCAGCCACCTGGGACTTCGACGACGAGGCATTCGATGAGATCTCCGACGATGCCA AGGATTTCATCAGCAATCTGCTGAAGAAAGATATGAAAAACCGCCTGGACTGCACGCAGT GCCTTCAGCATCCATGGCTAATGAAAGATACCAAGAACATGGAGGCCAAGAAACTCTCCA AGGACCGGATGAAGAAGTACATGGCAAGAAGGAAATGGCAGAAAACGGGCAATGCTGTGA GAGCCATTGGAAGACTGTCCTCTATGGCAATGATCTCAGGGCTCAGTGGCAGGAAATCCT CAACAGGGTCACCAACCAGCCCGCTCAATGCAGAAAAACTAGAATCTGAAGAAGATGTGT CCCAAGCTTTCCTTGAGGCTGTTGCTGAGGAAAAGCCTCATGTAAAACCCTATTTCTCTA AGACCATTCGCGATTTAGAAGTTGTGGAGGGAAGTGCTGCTAGATTTGACTGCAAGATTG AAGGATACCCAGACCCCGAGGTTGTCTGGTTCAAAGATGACCAGTCAATCAGGGAGTCCC GCCACTTCCAGATAGACTACGATGAGGACGGGAACTGCTCTTTAATTATTAGTGATGTTT GCGGGGATGACGATGCCAAGTACACCTGCAAGGCTGTCAACAGTCTTGGAGAAGCCACCT GCACAGCAGAGCTCATTGTGGAAACGATGGAGGAAGGTGAAGGGGAAGGGGAAGAGGAAG AAGAGTGAAACAAAGCCAGAGAAAAGCAGTTTCTAAGTCATATTAAAAGGACTATTTCTC TAAAACTCAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_053029 unedited
GGACCCTTGTATACGACTCCTATAGGGCGGCCGCGTAATTCGGCACGAGGACCATGGGGG ATGTGAAGCTGGTTGCCTCGTCACACATTTCCAAAACCTCCCTCAGTGTGGATCACTCAA GAGTTGACTCCATGCCCCTGACAGAGGCCCCTGCTTTCATTTTGCCCCCTCGGAACCTCT GCATCAAAGAAGGAGCCACCGCCAAGTTCGAAGGGCGGGTCCGGGGTTACCCAGAGCCCC AGGTGACATGGCACAGAAACGGGCAACCCATCACCAGCGGGGGCCGCTTCCTGCTGGATT GCGGCATCCGGGGGACTTTCAGCCTTGTGATTCATGCTGTCCATGAGGAGGACAGGGGAA AGTATACCTGTGAAGCCACCAATGGCAGTGGTGCTCGCCAGGTGACAGTGGAGTTGACAG TAGAAGGAAGTTTTGCGAAGCAGCTTGGTCAGCCTGTTGTTTCCAAAACCTTAGGGGATA GATTTTCAGCTCCAGCAGTGGAGACCCGTCCTAGCATCTGGGGGGAGTGCCCACCAAAGT TTGCTACCAAGCTGGGCCGAGTTGTGGTCAAAGAAGGACAGATGGGACGATTCTCCTGCA AGATCACTGGCCGGCCCCAACCGCAGGTCACCTGGCTCAAGGGAAATGTTCCACTGCAGC CGAGTGCCCGTGTGTCTGTGTCTGAGAAGAACGGCATGCAGTTCTGGAAATCCATGGAGT CAACCAAGATGACGTGGGAGTGTACACGTGCCTGGTGGTGAACGGGTCGGGGAAAGGCCT CGATGTCAGCTGAACTTTCCATCCAAGTTTGGACAGTGCCCAATAGGTCATTTGTGAGAG AAACAAAAAGCCACCAATTCAGATGTCAGAAAGAGTGACCATGTATCTCAAAGAGTCGAG CTGACAGTCTGGGAGGCTGCAGCCAAAAGCAG >OriGene 3' read for NM_053029 unedited
CGGCGCAGGCACCTTCAGGGCCGGAAGGCACCGGGGAGGGGTCACAGGGACTGCCACCCG GGATCTGTTCAGGAAACAGCTATTGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTT TTTTTTTTGAGTTTTAGAGAAATAGTCCTTTTAATATGACTTAGAAACTGCTTTTCTCTG GCTTTGTTTCACTCTTCTTCCTCTTCCCCTTCCCCTTCACCTTCCTCCATCGTTTCCACA ATGAGCTCTGCTGTGCAGGTGGCTTCTCCAAGACTGTTGACAGCCTTGCAGGTGTACTTG GCATCGTCATCCCCGCAAACATCACTAATAATTAAAGAGCAGTTCCCGTCCTCATCGTAG TCTATCTGGAAGTGGCGGGACTCCCTGATTGACTGGTCATCTTTGAACCAGACAACCTCG GGGTCTGGGTATCCTTCAATCTTGCAGTCAAATCTAGCAGCACTTCCCTCCACAACTTCT AAATCGCGAATGGTCTTAGAGAAATAGGGTTTTACATGAGGCTTTTCCTCAGCAACAGCC TCAAGGAAAGCTTGGGACACATCTTCTTCAGATTCTAGTTTTTCTGCATTGAGCGGGCTG GTTGGTGACCCTGTTGAGGATTTCCTGCCACTGAGCCCTGAGATCATTGCCATAGAGGAC AGTCTTCCAATGGCTCTCACAGCATTGCCCGTTTTCTGCCATTTCCTTCTTGCCATGTAC TTCTTCATCCGGTCCTTGGAGAGTTTCTTGGCCCTCCATGTTCTTGGTATCTTTCATTAG CCATGGATGCTGAAAGCACTGCGTGCAGTCCAGGCGGTTTTTCATATCTTTCTTCAGCAG ATGCTGATGAAATCTTGCATCGTCGAGATCTCATCGATGCCTCGTCGTCGAAGTCCCAGG TGGCTGAGGTAACGTTTGGCCCAAGGTTTCGTAAT |
| Restriction Sites | Please inquire |
| ACCN | NM_053029 |
| Insert Size | 5600 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | There is 5 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 5 aa. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_053029.2, NP_444257.2 |
| RefSeq Size | 7632 bp |
| RefSeq ORF | 7632 bp |
| Locus ID | 4638 |
| Domains | pkinase, TyrKc, S_TKc, ig, IGc2, IG, FN3 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Calcium signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Vascular smooth muscle contraction |
| Gene Summary | This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (4) does not utilize exon 27 and encodes the shortest nonmuscle isoform. |
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