United States
Germany
Japan
United Kingdom
China
Myosin light chain kinase (MYLK) (NM_053026) Human Untagged Clone
CAT＃: SC309073
MYLK (untagged)-Human myosin light chain kinase (MYLK), transcript variant 2
CNY 35,530.00
货期*
9周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210; MMIHS; MMIHS1; MSTP083; MYLK1; smMLCK |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_053026, the custom clone sequence may differ by one or more nucleotides
ATGGGGGATGTGAAGCTGGTTGCCTCGTCACACATTTCCAAAACCTCCCTCAGTGTGGAT CCCTCAAGAGTTGACTCCATGCCCCTGACAGAGGCCCCTGCTTTCATTTTGCCCCCTCGG AACCTCTGCATCAAAGAAGGAGCCACCGCCAAGTTCGAAGGGCGGGTCCGGGGTTACCCA GAGCCCCAGGTGACATGGCACAGAAACGGGCAACCCATCACCAGCGGGGGCCGCTTCCTG CTGGATTGCGGCATCCGGGGGACTTTCAGCCTTGTGATTCATGCTGTCCATGAGGAGGAC AGGGGAAAGTATACCTGTGAAGCCACCAATGGCAGTGGTGCTCGCCAGGTGACAGTGGAG TTGACAGTAGAAGGAAGTTTTGCGAAGCAGCTTGGTCAGCCTGTTGTTTCCAAAACCTTA GGGGATAGATTTTCAGCTCCAGCAGTGGAGACCCGTCCTAGCATCTGGGGGGAGTGCCCA CCAAAGTTTGCTACCAAGCTGGGCCGAGTTGTGGTCAAAGAAGGACAGATGGGACGATTC TCCTGCAAGATCACTGGCCGGCCCCAACCGCAGGTCACCTGGCTCAAGGGAAATGTTCCA CTGCAGCCGAGTGCCCGTGTGTCTGTGTCTGAGAAGAACGGCATGCAGGTTCTGGAAATC CATGGAGTCAACCAAGATGACGTGGGAGTGTACACGTGCCTGGTGGTGAACGGGTCGGGG AAGGCCTCGATGTCAGCTGAACTTTCCATCCAAGGTTTGGACAGTGCCAATAGGTCATTT GTGAGAGAAACAAAAGCCACCAATTCAGATGTCAGGAAAGAGGTGACCAATGTAATCTCA AAGGAGTCGAAGCTGGACAGTCTGGAGGCTGCAGCCAAAAGCAAGAACTGCTCCAGCCCC CAGAGAGGTGGCTCCCCACCCTGGGCTGCAAACAGCCAGCCTCAGCCCCCAAGGGAGTCC AAGCTGGAGTCATGCAAGGACTCGCCCAGAACGGCCCCGCAGACCCCGGTCCTTCAGAAG ACTTCCAGCTCCATCACCCTGCAGGCCGCAAGAGTTCAGCCGGAACCAAGAGCACCAGGC CTGGGGGTCCTATCACCTTCTGGAGAAGAGAGGAAGAGGCCAGCTCCTCCCCGTCCAGCC ACCTTCCCCACCAGGCAGCCTGGCCTGGGGAGCCAAGATGTTGTGAGCAAGGCTGCTAAC AGGAGAATCCCCATGGAGGGCCAGAGGGATTCAGCATTCCCCAAATTTGAGAGCAAGCCC CAAAGCCAGGAGGTCAAGGAAAATCAAACTGTCAAGTTCAGATGTGAAGGGCTTGCCGTG ATGGAGGTGGCCCCCTCCTTCTCCAGTGTCCTGAAGGACTGCGCTGTTATTGAGGGCCAG GATTTTGTGCTGCAGTGCTCCGTACGGGGGACCCCAGTGCCCCGGATCACTTGGCTGCTG AATGGGCAGCCCATCCAGTACGCTCGCTCCACCTGCGAGGCCGGCGTGGCTGAGCTCCAC ATCCAGGATGCCCTGCCGGAGGACCATGGCACCTACACCTGCCTAGCTGAGAATGCCTTG GGGCAGGTGTCCTGCAGCGCCTGGGTCACCGTCCATGAAAAGAAGAGTAGCAGGAAGAGT GAGTACCTTCTGCCTGTGGCTCCCAGCAAGCCCACTGCACCCATCTTCCTGCAGGGCCTC TCTGATCTCAAAGTCATGGATGGAAGCCAGGTCACTATGACTGTCCAAGTGTCAGGGAAT CCACCCCCTGAAGTCATCTGGCTGCACAATGGGAATGAGATCCAAGAGTCAGAGGACTTC CACTTTGAACAGAGAGGAACTCAGCACAGCCTTTGTATCCAGGAAGTGTTCCCGGAGGAC ACGGGCACGTACACCTGCGAGGCCTGGAACAGCGCTGGAGAGGTCCGCACCCAGGCCGTG CTCACGGTACAAGAGCCTCACGATGGCACCCAGCCCTGGTTCATCAGTAAGCCTCGCTCA GTGACAGCCTCCCTGGGCCAGAGTGTCCTCATCTCCTGCGCCATAGCTGGTGACCCCTTT CCTACCGTGCACTGGCTCAGAGATGGCAAAGCCCTCTGCAAAGACACTGGCCACTTCGAG GTGCTTCAGAATGAGGACGTGTTCACCCTGGTTCTAAAGAAGGTGCAGCCCTGGCATGCC GGCCAGTATGAGATCCTGCTCAAGAACCGGGTTGGCGAATGCAGTTGCCAGGTGTCACTG ATGCTACAGAACAGCTCTGCCAGAGCCCTTCCACGGGGGAGGGAGCCTGCCAGCTGCGAG GACCTCTGTGGTGGAGGAGTTGGTGCTGATGGTGGTGGTAGTGACCGCTATGGGTCCCTG AGGCCTGGCTGGCCAGCAAGAGGGCAGGGTTGGCTAGAGGAGGAAGACGGCGAGGACGTG CGAGGGGTGCTGAAGAGGCGCGTGGAGACGAGGCAGCACACTGAGGAGGCGATCCGCCAG CAGGAGGTGGAGCAGCTGGACTTCCGAGACCTCCTGGGGAAGAAGGTGAGTACAAAGACC CTATCGGAAGACGACCTGAAGGAGATCCCAGCCGAGCAGATGGATTTCCGTGCCAACCTG CAGCGGCAAGTGAAGCCAAAGACTGTGTCTGAGGAAGAGAGGAAGGTGCACAGCCCCCAG CAGGTCGATTTTCGCTCTGTCCTGGCCAAGAAGGGGACTTCCAAGACCCCCGTGCCTGAG AAGGTGCCACCGCCAAAACCTGCCACCCCGGATTTTCGCTCAGTGCTGGGTGGCAAGAAG AAATTACCAGCAGAGAATGGCAGCAGCAGTGCCGAGACCCTGAATGCCAAGGCAGTGGAG AGTTCCAAGCCCCTGAGCAATGCACAGCCTTCAGGGCCCTTGAAACCCGTGGGCAACGCC AAGCCTGCTGAGACCCTGAAGCCAATGGGCAACGCCAAGCCTGCCGAGACCCTGAAGCCC ATGGGCAATGCCAAGCCTGATGAGAACCTGAAATCCGCTAGCAAAGAAGAACTCAAGAAA GACGTTAAGAATGATGTGAACTGCAAGAGAGGCCATGCAGGGACCACAGATAATGAAAAG AGATCAGAGAGCCAGGGGACAGCCCCAGCCTTCAAGCAGAAGCTGCAAGATGTTCATGTG GCAGAGGGCAAGAAGCTGCTGCTCCAGTGCCAGGTGTCTTCTGACCCCCCAGCCACCATC ATCTGGACGCTGAACGGAAAGACCCTCAAGACCACCAAGTTCATCATCCTCTCCCAGGAA GGCTCACTCTGCTCCGTCTCCATCGAGAAGGCACTGCCTGAGGACAGAGGCTTATACAAG TGTGTAGCCAAGAATGACGCTGGCCAGGCGGAGTGCTCCTGCCAAGTCACCGTGGATGAT GCTCCAGCCAGTGAGAACACCAAGGCCCCAGAGATGAAATCCCGGAGGCCCAAGAGCTCT CTTCCTCCCGTGCTAGGAACTGAGAGTGATGCGACTGTGAAAAAGAAACCTGCCCCCAAG ACACCTCCGAAGGCAGCAATGCCCCCTCAGATCATCCAGTTCCCTGAGGACCAGAAGGTA CGCGCAGGAGAGTCAGTGGAGCTGTTTGGCAAAGTGACAGGCACTCAGCCCATCACCTGT ACCTGGATGAAGTTCCGAAAGCAGATCCAGGAAAGCGAGCACATGAAGGTGGAGAACAGC GAGAATGGCAGCAAGCTCACCATCCTGGCCGCGCGCCAGGAGCACTGCGGCTGCTACACA CTGCTGGTGGAGAACAAGCTGGGCAGCAGGCAGGCCCAGGTCAACCTCACTGTCGTGGAT AAGCCAGACCCCCCAGCTGGCACACCTTGTGCCTCTGACATTCGGAGCTCCTCACTGACC CTGTCCTGGTATGGCTCCTCATATGATGGGGGCAGTGCTGTACAGTCCTACAGCATCGAG ATCTGGGACTCAGCCAACAAGACGTGGAAGGAACTAGCCACATGCCGCAGCACCTCTTTC AACGTCCAGGACCTGCTGCCTGACCACGAATATAAGTTCCGTGTACGTGCAATCAACGTG TATGGAACCAGTGAGCCAAGCCAGGAGTCTGAACTCACAACGGTAGGAGAGAAACCTGAA GAGCCGAAGGATGAAGTGGAGGTGTCAGATGATGATGAGAAGGAGCCCGAGGTTGATTAC CGGACAGTGACAATCAATACTGAACAAAAAGTATCTGACTTCTACGACATTGAGGAGAGA TTAGGATCTGGGAAATTTGGACAGGTCTTTCGACTTGTAGAAAAGAAAACTCGAAAAGTC TGGGCAGGGAAGTTCTTCAAGGCATATTCAGCAAAAGAGAAAGAGAATATCCGGCAGGAG ATTAGCATCATGAACTGCCTCCACCACCCTAAGCTGGTCCAGTGTGTGGATGCCTTTGAA GAAAAGGCCAACATCGTCATGGTCCTGGAGATCGTGTCAGGAGGGGAGCTGTTTGAGCGC ATCATTGACGAGGACTTTGAGCTGACGGAGCGTGAGTGCATCAAGTACATGCGGCAGATC TCGGAGGGAGTGGAGTACATCCACAAGCAGGGCATCGTGCACCTGGACCTCAAGCCGGAG AACATCATGTGTGTCAACAAGACGGGCACCAGGATCAAGCTCATCGACTTTGGTCTGGCC AGGAGGCTGGAGAATGCGGGGTCTCTGAAGGTCCTCTTTGGCACCCCAGAATTTGTGGCT CCTGAAGTGATCAACTATGAGCCCATCGGCTACGCCACAGACATGTGGAGCATCGGGGTC ATCTGCTACATCCTAGTCAGTGGCCTTTCCCCCTTCATGGGAGACAACGATAACGAAACC TTGGCCAACGTTACCTCAGCCACCTGGGACTTCGACGACGAGGCATTCGATGAGATCTCC GACGATGCCAAGGATTTCATCAGCAATCTGCTGAAGAAAGATATGAAAAACCGCCTGGAC TGCACGCAGTGCCTTCAGCATCCATGGCTAATGAAAGATACCAAGAACATGGAGGCCAAG AAACTCTCCAAGGACCGGATGAAGAAGTACATGGCAAGAAGGAAATGGCAGAAAACGGGC AATGCTGTGAGAGCCATTGGAAGACTGTCCTCTATGGCAATGATCTCAGGGCTCAGTGGC AGGAAATCCTCAACAGGGTCACCAACCAGCCCGCTCAATGCAGAAAAACTAGAATCTGAA GAAGATGTGTCCCAAGCTTTCCTTGAGGCTGTTGCTGAGGAAAAGCCTCATGTAAAACCC TATTTCTCTAAGACCATTCGCGATTTAGAAGTTGTGGAGGGAAGTGCTGCTAGATTTGAC TGCAAGATTGAAGGATACCCAGACCCCGAGGTTGTCTGGTTCAAAGATGACCAGTCAATC AGGGAGTCCCGCCACTTCCAGATAGACTACGATGAGGACGGGAACTGCTCTTTAATTATT AGTGATGTTTGCGGGGATGACGATGCCAAGTACACCTGCAAGGCTGTCAACAGTCTTGGA GAAGCCACCTGCACAGCAGAGCTCATTGTGGAAACGATGGAGGAAGGTGAAGGGGAAGGG GAAGAGGAAGAAGAGTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_053026 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_053026.2, NP_444254.2 |
| RefSeq Size | 7645 bp |
| RefSeq ORF | 5538 bp |
| Locus ID | 4638 |
| UniProt ID | Q15746 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Calcium signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Vascular smooth muscle contraction |
| Gene Summary | This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) does not utilize exon 11, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
其它Myosin light chain kinase产品
Customer
Reviews
Loading...
