MED12L (NM_053002) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NIZIDS; NOPAR; TNRC11L; TRALP; TRALPUSH
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC309072 representing NM_053002. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCGCCTTCGGGCTTCTCAGCTATGAGCAGAGACCGCTGAAGCGCCCCCGGCTCGGGCCGCCCGAC GTCTACCCACAGGACCCCAAGCAGAAGGAGGATGAACTTACTGCTGTGAATGTAAAGCAAGGCTTCAAT AATCAGCCAGCCTTCACTGGAGATGAACATGGCTCAGCCAGAAATATTGTAATTAACCCATCAAAGATT GGAGCTTATTTTAGCAGCATATTAGCTGAGAAACTGAAGCTTAACACTTTCCAGGACACGGGAAAGAAG AAACCACAAGTTAATGCTAAAGATAATTATTGGCTGGTTACTGCTCGATCCCAGAGTGCAATTCATAGT TGGTTTTCTGACTTAGCAGGAAATAAGCCACTTTCTATTTTGGCAAAAAAGGTTCCTATCCTTAGTAAA AAAGAGGATGTTTTTGCATATTTAGCTAAATATTCTGTGCCAATGGTTCGAGCAACGTGGCTGATCAAG ATGACTTGTGCCTATTATTCTGCTATATCTGAAGCTAAAATTAAGAAACGTCAGGCTCCTGATCCGAAT TTGGAGTGGACACAGATATCTACCAGATATCTTCGAGAGCAGTTGGCCAAGATTTCTGACTTTTACCAC ATGGCCTCCAGCACGGGCGATGGCCCTGTCCCTGTGCCACCAGAGGTGGAGCAAGCCATGAAGCAATGG GAATACAACGAAAAGCTAGCATTTCACATGTTCCAGGAAGGAATGTTAGAAAAACACGAATATTTGACA TGGATCCTGGATGTTTTAGAAAAGATCAGACCAATGGATGATGATCTTCTTAAACTCTTGCTACCACTA ATGCTGCAGTATTCAGATGAGTTTGTTCAGTCGGCCTACCTGTCTCGTCGTCTTGCCTACTTTTGTGCC CGGCGTCTTTCCTTGCTGCTGAGCGATAGCCCCAACCTCCTTGCTGCCCACTCACCCCACATGATGATA GGACCAAACAACTCGAGTATCGGGGCCCCCAGCCCTGGCCCCCCCGGCCCTGGCATGAGCCCCGTGCAG CTGGCCTTCTCAGATTTTCTTTCCTGTGCACAGCATGGTCCCCTGGTTTATGGACTTAGTTGTATGTTG CAGACTGTCACTCTCTGTTGCCCAAGTGCCTTGGTGTGGAATTATTCCACAAATGAAAATAAGAGCGCA AACCCAGGCTCACCCCTGGATCTGCTGCAGGTGGCCCCGTCCAGCCTCCCCATGCCGGGTGGGAACACG GCTTTCAATCAGCAGGTTCGGGCAAGGATTTATGAAGTAGAACAACAGATAAAACAAAGAGGCCGTGCA GTGGAAGTTCGGTGGTCATTTGACAAGTGCCAAGAATCCACAGCAGGGGTGACTATTAGTCGGGTTTTG CACACGTTGGAAGTTTTGGATCGTCACTGTTTTGACCGAACTGATTCCAGCAATTCCATGGAGACACTT TATCATAAGATTTTCTGGGCAAACCAAAACAAAGATAACCAAGAGGTTGCGCCCAACGATGAAGCTGTG GTGACGCTGTTATGTGAATGGGCCGTGAGCTGCAAACGGTCTGGCAAGCACAGGGCCATGGCTGTGGCA AAACTCCTGGAGAAGAGGCAAGCAGAAATTGAGGCAGAGAGATGTGGTGAATCAGAAGTCTTAGATGAG AAGGAGTCTATTTCTTCATCCTCTCTTGCTGGATCCAGTTTGCCTGTTTTCCAGAATGTGCTGTTAAGG TTTTTAGATACACAGGCCCCCTCTTTGTCGGACCCAAACAGTGAATGTGAAAAGGTGGAATTTGTGAAC CTGGTGCTGCTCTTCTGCGAGTTCATCCGCCATGATGTCTTCTCCCATGACGCATACATGTGTACCCTC ATATCTCGAGGAGATTTGTCAGTCACTGCCTCAACTCGGCCGCGGTCACCAGTAGGGGAAAATGCAGAT GAACACTATTCAAAGGACCATGATGTGAAAATGGAGATTTTTTCTCCTATGCCTGGAGAATCCTGTGAG AATGCCAACACTTCGTTGGGCAGAAGAATGTCAGTTAATTGTGAGAAGTTGGTGAAGAGGGAAAAGCCA AGGGAATTAATTTTTCCATCTAATTATGACCTCCTTCGCCACTTACAGTATGCAACACATTTTCCTATA CCTCTGGATGAATCTTCAAGTCATGAATGTAACCAGCGCACAATCCTTCTCTATGGAGTCGGCAAAGAG CGTGATGAAGCAAGGCATCAGCTGAAGAAGATTACCAAAGATATCCTGAAAATTCTAAATAAGAAGAGC ACCACAGAGACAGGGGTTGGGGACGAAGGACAAAAAGCCAGGAAGAACAAACAGGAGACATTTCCAACA CTGGAGACTGTGTTCACTAAACTCCAGCTCCTTTCATATTTTGATCAACATCAAGTGACATCTCAGATT TCTAACAATGTGCTAGAACAAATCACAAGCTTTGCGTCAGGAACATCCTATCATCTCCCTTTGGCTCAC CACATTCAGCTCATCTTTGATCTCATGGAGCCAGCACTGAACATCAACGGACTAATTGACTTCGCAATA CAGTTACTAAATGAACTGAGTGTTGTGGAAGCTGAACTGCTCCTAAAATCCTCCAGCCTGGCAGGAAGT TATACAACAGGACTGTGTGTCTGCATCGTGGCTGTTCTCAGGCGCTATCACAGTTGTCTAATCTTGAAT CCTGATCAGACAGCCCAGGTGTTTGAAGGGTTGTGTGGTGTGGTCAAGCATGTCGTAAACCCCTCAGAA TGTTCTTCCCCTGAAAGATGCATTTTAGCCTACCTCTATGATCTCTATGTGTCATGTAGCCACCTCAGA AGTAAATTTGGAGACCTCTTCAGTAGTGCCTGTTCAAAAGTAAAGCAAACCATATATAATAACGTGATG CCTGCAAATTCGAACTTGCGATGGGATCCAGACTTCATGATGGATTTTATTGAGAATCCCTCAGCCCGC AGCATCAACTACTCAATGCTGGGCAAGATCCTCAGTGACAATGCGGCCAATCGCTACAGCTTTGTCTGC AATACACTCATGAATGTATGTATGGGCCATCAGGATGCTGGCAGGATTAACGACATAGCCAATTTCTCC TCTGAGCTTACGGCTTGCTGCACTGTTCTTAGTTCAGAATGGCTGGGGGTTCTGAAGGCTCTTTGTTGT TCTTCAAATCACGTGTGGGGGTTTAATGATGTACTTTGCACTGTAGATGTGAGTGACCTTTCATTCCAT GATTCATTAGCTACTTTCATTGCTATTCTGATAGCACGACAGTGTTTTTCCCTGGAGGACGTCGTGCAG CATGTCGCACTTCCCTCTCTTCTAGCAGCAGCTTGTGGGGATGCGGACGCCGAGCCTGGGGCGAGAATG ACATGCCGACTCTTGCTTCATCTCTTCCGAGCTCCCCAGGCCTGCTTCTTACCTCAAGCAACGGGCAAA CCTTTCCCTGGAATAAGATCATCTTGTGATAGACACCTCTTAGCCGCTGCTCACAACAGCATTGAAGTG GGAGCCGTGTTTGCTGTCTTAAAAGCAATTATGATGCTTGGAGATGCCAAAATTGGCAATAACAGTGTC AGCTCTTTAAAGAATGATGACTTCACCATGAGAGGTTTGCGATGTGATGGGAATGCTGATGATATCTGG ACTGCCTCACAAAATCCAAAATCCTGTGGGAAAAGCATTTCCATAGAAACTGCCAATTTAAGAGAATAC GCTAGATATGTACTGAGGACTATCTGTCAACAGGAATGGGTAGGAGAGCATTGCTTAAAAGAACCTGAA AGATTATGTACAGACAAAGAACTTATATTGGACCCTGTGCTTTCAAATATGCAAGCACAGAAATTACTG CAGCTTATCTGTTATCCTCATGGCATTAAAGAATGTACCGAGGGGGACAATCTGCAAAGACAGCACATT AAGCGTATTCTTCAGAATCTTGAGCAGTGGACACTGAGGCAATCCTGGTTAGAACTCCAGCTAATGATC AAACAGTGCTTGAAGGACCCTGGCTCTGGTTCTGTGGCCGAAATGAACAACTTACTGGACAATATTGCA AAGGCAACAATAGAGGTATTCCAGCAGTCTGCAGACCTAAATAATTCTTCTAATTCTGGCATGAGCCTC TTCAACCCAAACAGTATTGGAAGTGCTGATACAAGTAGCACGAGACAGAATGGAATAAAGACATTCCTA AGTTCCTCCGAACGCAGGGGTGTATGGTTGGTGGCCCCCCTCATCGCCAGGTTGCCAACTTCTGTGCAA GGAAGAGTGCTGAAAGCCGCTGGGGAAGAGCTGGAGAAGGGACAGCACTTGGGTTCTTCTTCCAAAAAG GAAAGGGACAGACAGAAACAGAAAAGTATGTCTCTTTTGAGTCAACAACCCTTCCTCTCACTGGTACTT ACCTGCCTTAAGGGACAAGATGAACAAAGGGAAGGCCTCCTAACATCTCTCCAGAATCAAGTTAACCAG ATTTTAAGTAACTGGAGAGAAGAACGATACCAAGATGACATAAAAGCGCGGCAGATGATGCACGAAGCA TTGCAACTCCGCCTAAATTTGGTAGGAGGAATGTTTGACACGGTGCAGAGGAGCACCCAGTGGACTACA GACTGGGCCCTGCTACTCCTTCAGATCATTACTTCAGGAACTGTTGACATGCACACTAACAATGAATTA TTCACAACAGTTCTTGACATGCTGGGTGTTTTAATCAATGGAACGTTAGCCTCTGACCTATCAAATGCA TCCCCTGGGGGATCTGAAGAGAACAAGCGTGCATACATGAATTTAGTAAAGAAACTGAAAAAAGAGCTA GGAGACAAGCGATCAGAAAGTATTGACAAAGTTCGACAGTTACTACCTTTGCCGAAACAGACATGTGAT GTCATCACTTGTGAACCTATGGGTTCCTTGATTGACACAAAAGGAAACAAAATTGCTGGATTTGACTCT ATAGATAAAAAACAGGGTCTCCAGGTCTCTACGAAGCAGAAGGTGTCCCCGTGGGACTTGTTTGAGGGT CAGAAGAACCCAGCTCCTTTGTCCTGGGCCTGGTTTGGGACAGTCCGAGTGGACCGAAGAGTGATCAAG TACGAGGAGCAGCATCACCTCCTGCTGTATCACACACACCCCATGCCCAAGCCCCGCAGTTACTACCTC CAGCCACTGCCCCTGCCTCCTGAGGAGGAAGAGGAAGAGCCCACATCTCCAGTTTCTCAGGAACCAGAA AGGAAGTCCGCTGAGCTGTCAGATCAGGGAAAAACCACAACAGATGAAGAAAAGAAAACAAAAGGAAGG AAGCGCAAGACGAAATCTAGCTCAAGAGTTGATGAATATCCACAGAGCAACATATACCGAGTGCCTCCT AATTACTCGCCTATCTCCTCCCAAATGATGCACCATCCACAGTCCACCTTGTGGGGTTACAACCTCGTG GGCCAGCCCCAGCAGCCCGGCTTTTTCCTTCAGAACCAATCTCTTACTCCAGGTGGCTCCAGATTGGAC CCTGCAGGCTCCTTTGTCCCAACCAACACCAAACAAGCTCTGTCAAACATGCTACAGCGGCGCTCAGGC GCCATGATGCAGCCGCCTTCTCTTCATGCAATCACATCGCAGCAGCAGTTGATACAGATGAAGCTTCTG CAGCAGCAGCAGCAACAGCGACTTCTCAGGCAAGCCCAGACTCGGCCTTTCCAACAGGGCCAGCCGGGG GACCAGGCTGCTCTCTTTGCTGCGCAAGCACGGCCCTCCCCTCAGCTCCCTCAGTATCCAGGGCTGCAG CAAGCACAGACCATGCCACAGGGCTATACAATGTATGGGACACAGATGCCTTTGCAGCAGACATCGCAG CAGCAGGCTGGCAGTGTGGTCCTGTCTCCCAGCTATAACTCCAGAGCCTATCCGGCCGCACATTCCAAC CCCGTGCTAATGGAAAGACTCAGACAGATTCAGCAGCAGCCGAGTGGCTATGTTCAGCAGCAGGCCTCG CCGTACCTGCAGCCCCTGACTGGCTCTCAGAGACTGAACCATCAGGCTCTACAGCAGAGCCCTCTGGTG GGCGGGGGAATTGATGCTGTGCTGACTTCTGCACATCCAAACCTTCCCTCCGTGCCCCTGCCTCAGGAT CCCATGAGACCCAGACAGCCGCAAGTTCGACAGCAGCAGAGACTCCTCCAGATGCAGCAGCCCCAGCAG CCCCAGCCCCAGCAGCCTCCCCAGCCCCAGCAGTCCTCGCAGTCCCAGAGTCAGACCCTTGGTCTCCAA GCAATGCAGCCCCAGCAGCCCTTGTTTCCCAGGCAAGGCTTGCAGCAGACCCAGCAGCAGCAGCAGACG GCCGCCTTGGTGCGGCAGCTCCAGAAGCAGCTTTCCAGCAACCAGCCACAGCAAGGAGTGACTCCGTAT GGGCATCCTTCACACTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_053002
Insert Size	6438 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053002.5
RefSeq Size	10440 bp
RefSeq ORF	6438 bp
Locus ID	116931
UniProt ID	Q86YW9
MW	240.1 kDa
Gene Summary	The protein encoded by this gene is part of the Mediator complex, which is involved in transcriptional coactivation of nearly all RNA polymerase II-dependent genes. The Mediator complex links gene-specific transcriptional activators with the basal transcription machinery. [provided by RefSeq, May 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.