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UBE3A (NM_000462) Human Untagged Clone
CAT＃: SC309057
UBE3A (untagged)-Human ubiquitin protein ligase E3A (UBE3A), transcript variant 2
CNY 6,416.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ANCR; AS; E6-AP; EPVE6AP; HPVE6A |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_000462 edited
ACCAGCTCCTCAGAAGTTTGGCGAAATATGAGTTATTAAGCCTACGCTCAGATCAAGGTA GCAGCTAGACTGGTGTGACAACCTGTTTTTAATCAGTGACTCAAAGCTGTGATCACCCTG ATGTCACCGAATGGCCACAGCTTGTAAAAGAGAGTTACAGTGGAGGTAAAAGGAGTGGCT TGCAGGATGGAGAAGCTGCACCAGTGTTATTGGAAATCAGGAGAACCTCAGTCTGACGAC ATTGAAGCTAGCCGAATGAAGCGAGCAGCTGCAAAGCATCTAATAGAACGCTACTACCAC CAGTTAACTGAGGGCTGTGGAAATGAAGCCTGCACGAATGAGTTTTGTGCTTCCTGTCCA ACTTTTCTTCGTATGGATAATAATGCAGCAGCTATTAAAGCCCTCGAGCTTTATAAGATT AATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCTTACCTTGAG AACTCGAAAGGTGCCCCCAACAACTCCTGCTCTGAGATAAAAATGAACAAGAAAGGCGCT AGAATTGATTTTAAAGATGTGACTTACTTAACAGAAGAGAAGGTATATGAAATTCTTGAA TTATGTAGAGAAAGAGAGGATTATTCCCCTTTAATCCGTGTTATTGGAAGAGTTTTTTCT AGTGCTGAGGCATTGGTACAGAGCTTCCGGAAAGTTAAACAACACACCAAGGAAGAACTG AAATCTCTTCAAGCAAAAGATGAAGACAAAGATGAAGATGAAAAGGAAAAAGCTGCATGT TCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCCTCAAGGATAGGTGATAGCTCA CAGGGAGACAACAATTTGCAAAAATTAGGCCCTGATGATGTGTCTGTGGATATTGATGCC ATTAGAAGGGTCTACACCAGATTGCTCTCTAATGAAAAAATTGAAACTGCCTTTCTCAAT GCACTTGTATATTTGTCACCTAACGTGGAATGTGACTTGACGTATCACAATGTATACTCT CGAGATCCTAATTATCTGAATTTGTTCATTATCGTAATGGAGAATAGAAATCTCCACAGT CCTGAATATCTGGAAATGGCTTTGCCATTATTTTGCAAAGCGATGAGCAAGCTACCCCTT GCAGCCCAAGGAAAACTGATCAGACTGTGGTCTAAATACAATGCAGACCAGATTCGGAGA ATGATGGAGACATTTCAGCAACTTATTACTTATAAAGTCATAAGCAATGAATTTAACAGT CGAAATCTAGTGAATGATGATGATGCCATTGTTGCTGCTTCGAAGTGCTTGAAAATGGTT TACTATGCAAATGTAGTGGGAGGGGAAGTGGACACAAATCACAATGAAGAAGATGATGAA GAGCCCATCCCTGAGTCCAGCGAGCTGACACTTCAGGAACTTTTGGGAGAAGAAAGAAGA AACAAGAAAGGTCCTCGAGTGGACCCCCTGGAAACTGAACTTGGTGTTAAAACCCTGGAT TGTCGAAAACCACTTATCCCTTTTGAAGAGTTTATTAATGAACCACTGAATGAGGTTCTA GAAATGGATAAAGATTATACTTTTTTCAAAGTAGAAACAGAGAACAAATTCTCTTTTATG ACATGTCCCTTTATATTGAATGCTGTCACAAAGAATTTGGGATTATATTATGACAATAGA ATTCGCATGTACAGTGAACGAAGAATCACTGTTCTCTACAGCTTAGTTCAAGGACAGCAG TTGAATCCATATTTGAGACTCAAAGTTAGACGTGACCATATCATAGATGATGCACTTGTC CGGCTAGAGATGATCGCTATGGAAAATCCTGCAGACTTGAAGAAGCAGTTGTATGTGGAA TTTGAAGGAGAACAAGGAGTTGATGAGGGAGGTGTTTCCAAAGAATTTTTTCAGCTGGTT GTGGAGGAAATCTTCAATCCAGATATTGGTATGTTCACATACGATGAATCTACAAAATTG TTTTGGTTTAATCCATCTTCTTTTGAAACTGAGGGTCAGTTTACTCTGATTGGCATAGTA CTGGGTCTGGCTATTTACAATAACTGTATACTGGATGTACATTTTCCCATGGTTGTCTAC AGGAAGCTAATGGGGAAAAAAGGAACTTTTCGTGACTTGGGAGACTCTCACCCAGTTCTA TATCAGAGTTTAAAAGATTTATTGGAGTATGAAGGGAATGTGGAAGATGACATGATGATC ACTTTCCAGATATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAGGAA AATGGTGATAAAATTCCAATTACAAATGAAAACAGGAAGGAATTTGTCAATCTTTATTCT GACTACATTCTCAATAAATCAGTAGAAAAACAGTTCAAGGCTTTTCGGAGAGGTTTTCAT ATGGTGACCAATGAATCTCCCTTAAAGTACTTATTCAGACCAGAAGAAATTGAATTGCTT ATATGTGGAAGCCGGAATCTAGATTTCCAAGCACTAGAAGAAACTACAGAATATGACGGT GGCTATACCAGGGACTCTGTTCTGATTAGGGAGTTCTGGGAAATCGTTCATTCATTTACA GATGAACAGAAAAGACTCTTCTTGCAGTTTACAACGGGCACAGACAGAGCACCTGTGGGA GGACTAGGAAAATTAAAGATGATTATAGCCAAAAATGGCCCAGACACAGAAAGGTTACCT ACATCTCATACTTGCTTTAATGTGCTTTTACTTCCGGAATACTCAAGCAAAGAAAAACTT AAAGAGAGATTGTTGAAGGCCATCACGTATGCCAAAGGATTTGGCATGCTGTAAAACAAA ACAAAACAAAATAAAACAAAAAAAAGGAAAAAAAAAA >OriGene 5' read for NM_000462 unedited
GTCCAGTGCATATTTGTATACGACTCATATAGGCGGCCGCGTAATTCGCACGAGGCTGAT TTTAGAATTCACCAGCTCCTCAGAATTTGGCGAAATATGAGTTATTAAGCCTACGCTCAG ATCAAGGTAGCAGCTAGACTGGTGTGACAACCTGTTTTTAATCAGTGACTCAAAGCTGTG ATCACCCTGATGTCACCGAATGGCCACAGCTTGTAAAAGAGAGTTACAGTGGAGGTAAAA GGAGTGGCTTGCAGGATGGAGAAGCTGCACCAGTGTTATTGGAAATCAGGAGAACCTCAG TCTGACGACATTGAAGCTAGCCGAATGAAGCGAGCAGCTGCAAAGCATCTAATAGAACGC TACTACCACCAGTTAACTGAGGGCTGTGGAAATGAAGCCTGCACGAATGAGTTTTGTGCT TCCTGTCCAACTTTTCTTCGTATGGATAATAATGCAGCAGCTATTAAAGCCCTCGAGCTT TATAAGATTAATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCT TACCTTGAGAACTCGAAAGGTGCCCCCAACAACTCCTGCTCTGAGATAAAAATGAACAAG ACAGGCGCTAGAATTGATTTTAAAGATGTGACTTACTTAACAGAAGAGAAGGTATATGAA ATTCTTGAATTATGTAGAGAAAGAGAGGATTATTCCCCTTTAATCCGTGTTATTGGAAGA GTTTTTTCTAGTGCTGAGGCATTGGTACAGAGCTTCCGNGAAGTTAAACAACACACCAAG GGAAGAACTGANNATCTCTTCAGCCANAGATGAAGACCAAGATGAAGATGAANAGGANAA AGCTGCNTGTTCTGCTGCTGCTATGGAAAGAGACTCANAAGCATCTTCTCA >Reverse primer walk for NM_000462 unedited
CCTGGNCCAATATGGCTGAAACCCCGTCTCTACCAAAAAAACAAAATTAGCTGAGCCTGG TCATGCATGCCTGNGAATCCCAACAACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACC CAGGAGGCGGAGATTGCAGTGAGCCAAGATTGTGCCACTGCACTCCAGCTTGGTTCCCAA TAGACCCCGCAGGCCCTACAGGTTGTCTTCCCAACTTGCCCCTTGCTCCATACCACCCCC CTCCACCCCATAATATTATAGAAGGACACCTAGTCAGACAAAATGATGCAACTTAATTTT ATTAGGACAAGGCTGGTGGGCACTGGAGTGGCAACTTCCAGGGCCAGGAGAGGCACTGGG GAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCA ATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTTATTTTGTTTTGGTTTGTTTTACAG CATGCCAAATCCTTTGGCATACGTGATGGCCTTCAACAATCTCTCTTTAAATTTTTCTTT GCTTGAGTCTTCCCGGAAGTAAAAGCCCATTAAAGCAAGTATGAGATGTAAGTAACCTTT CTGTGTCTGGGCCATTTTTGGGTATAATCATCTTTAAATTTCCCAGACCTCCCAAGGTGC TCTGGCTGTGCCCGTTGTAAACTGAAAAAGAATCCTTTTTGTTCATCTGTAAATAAAGGA ACAATTTCCCAAAACTCCTTAACCAAACAAAACCCTGGGTTAGCCCCCCGCCCAATTCTG GAATTTCTTCCCAAGCCTTGGAAATCAAAATCCCG |
| Restriction Sites | Please inquire |
| ACCN | NM_000462 |
| Insert Size | 2900 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000462.2, NP_000453.2 |
| RefSeq Size | 4516 bp |
| RefSeq ORF | 2628 bp |
| Locus ID | 7337 |
| UniProt ID | Q05086 |
| Domains | HECT |
| Protein Families | Druggable Genome |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) encodes the longest isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216727 | UBE3A (Myc-DDK-tagged)-Human ubiquitin protein ligase E3A (UBE3A), transcript variant 2 |
CNY 6,408.00 |
|
| RC216727L1 | Lenti ORF clone of Human ubiquitin protein ligase E3A (UBE3A), transcript variant 2, Myc-DDK-tagged |
CNY 8,808.00 |
|
| RC216727L2 | Lenti ORF clone of Human ubiquitin protein ligase E3A (UBE3A), transcript variant 2, mGFP tagged |
CNY 9,030.00 |
|
| RC216727L3 | Lenti ORF clone of Human ubiquitin protein ligase E3A (UBE3A), transcript variant 2, Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC216727L4 | Lenti ORF clone of Human ubiquitin protein ligase E3A (UBE3A), transcript variant 2, mGFP tagged |
CNY 9,030.00 |
|
| RG216727 | UBE3A (tGFP-tagged) - Human ubiquitin protein ligase E3A (UBE3A), transcript variant 2 |
CNY 8,008.00 |
