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ATP1B4 (NM_012069) Human Untagged Clone
CAT＃: SC308949
ATP1B4 (untagged)-Human ATPase, Na+/K+ transporting, beta 4 polypeptide (ATP1B4), transcript variant 2
CNY 5,700.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC308949 representing NM_012069.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGAAGGCAACTCCGGTCCAGAAGGGCTCCATCCTTTCCTTACAGTTATCGCTACAGACTCGATGAT CCGGATGAAGCGAACCAGAACTACTTAGCAGATGAAGAGGAGGAAGCAGAAGAAGAGGCTCGGGTGACG GTGGTGCCCAAATCGGAGGAGGAGGAAGAAGAGGAGGAGAAAGAAGAGGAGGAAGAGGAGGAAAAGGAG GAGGAAGAGGGTCAAGGTCAGCCAACAGGCAATGCCTGGTGGCAGAAATTGCAGATCATGAGTGAATAC CTGTGGGATCCAGAGAGAAGGATGTTTCTGGCCCGAACAGGCCTGATCTTACTCATTTACTTCTTCTTC TATGCCTCCTTGGCTGCTGTGATCACCCTCTGCATGTACACACTATTTCTGACCATCAGTCCCTATATA CCAACCTTCACGGAGCGGGTAAAGCCTCCTGGAGTTATGATCAGACCCTTCGCCCATAGCCTTAACTTC AACTTCAACGTTTCTGAACCCGACACTTGGCAGCATTATGTGATTAGCCTAAATGGCTTTCTCCAGGGT TATAATGACAGTCTTCAAGAGGAAATGAATGTAGATTGTCCCCCGGGGCAGTACTTCATCCAAGATGGC AATGAGGATGAGGACAAGAAGGCCTGCCAATTTAAGCGCTCCTTCCTAAAGAACTGCTCTGGTCTGGAG GACCCAACTTTTGGATACTCTACTGGACAGCCCTGCATCCTTCTAAAGATGAACCGGATTGTAGGCTTT CGTCCTGAGCTTGGAGATCCTGTGAAGGTTTCCTGCAAAGTTCAGAGAGGTGATGAAAATGACATCCGA TCCATCAGTTACTACCCAGAGTCGGCTTCTTTTGACCTCCGCTACTACCCTTACTACGGCAAACTGACT CACGTTAACTACACATCCCCCTTGGTGGCAATGCACTTTACAGACGTGGTGAAGAACCAAGCAGTGCCT GTGCAGTGCCAACTGAAGGGCAAAGGCGTCATAAATGATGTCATCAATGATCGTTTTGTGGGCAGGGTA ATCTTTACCCTGAACATAGAAACTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_012069 |
| Insert Size | 1062 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012069.4 |
| RefSeq Size | 4761 bp |
| RefSeq ORF | 1062 bp |
| Locus ID | 23439 |
| UniProt ID | Q9UN42 |
| Protein Families | Transmembrane |
| Protein Pathways | Cardiac muscle contraction |
| MW | 41.1 kDa |
| Gene Summary | This gene has been found in all vertebrate genomes sequenced to date. However, this gene has undergone a change in function in placental mammals compared to other species. Specifically, in fish, avian, and amphibian species, this gene encodes plasma membrane-bound beta-subunits of Na,K-ATPase. In placental mammals, the encoded protein interacts with the nuclear transcriptional coregulator SKIP and may be involved in the regulation of TGF-beta signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] Transcript Variant: This variant (2) uses an alternate in-frame splice site that deletes 12 nts in the 5' coding region, compared to variant 1. This variant encodes isoform B which is 4 aa shorter than isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224408 | ATP1B4 (Myc-DDK-tagged)-Human ATPase, Na+/K+ transporting, beta 4 polypeptide (ATP1B4), transcript variant 2 |
CNY 3,656.00 |
|
| RC224408L3 | Lenti ORF clone of Human ATPase, Na+/K+ transporting, beta 4 polypeptide (ATP1B4), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224408L4 | Lenti ORF clone of Human ATPase, Na+/K+ transporting, beta 4 polypeptide (ATP1B4), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG224408 | ATP1B4 (tGFP-tagged) - Human ATPase, Na+/K+ transporting, beta 4 polypeptide (ATP1B4), transcript variant 2 |
CNY 4,370.00 |
