NRG2 (NM_013981) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DON1; HRG2; NTAK
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >SC308933 representing NM_013981. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCGGCAGGTTTGCTGCTCAGCGCTGCCGCCGCCGCCACTGGAGAAGGGTCGGTGCAGCAGCTACAGC GACAGCAGCAGCAGCAGCAGCGAGAGGAGCAGCAGCAGCAGCAGCAGCAGCAGCGAGAGCGGCAGCAGC AGCAGGAGCAGCAGCAACAACAGCAGCATCTCTCGTCCCGCTGCGCCCCCAGAGCCGCGGCCGCAGCAA CAGCCGCAGCCCCGCAGCCCCGCAGCCCGGAGAGCCGCCGCCCGTTCGCGAGCCGCAGCCGCCGGCGGC ATGAGGCGCGACCCGGCCCCCGGCTTCTCCATGCTGCTCTTCGGTGTGTCGCTCGCCTGCTACTCGCCC AGCCTCAAGTCAGTGCAGGACCAGGCGTACAAGGCACCCGTGGTGGTGGAGGGCAAGGTACAGGGGCTG GTCCCAGCCGGCGGCTCCAGCTCCAACAGCACCCGAGAGCCGCCCGCCTCGGGTCGGGTGGCGTTGGTA AAGGTGCTGGACAAGTGGCCGCTCCGGAGCGGGGGGCTGCAGCGCGAGCAGGTGATCAGCGTGGGCTCC TGTGTGCCGCTCGAAAGGAACCAGCGCTACATCTTTTTCCTGGAGCCCACGGAACAGCCCTTAGTCTTT AAGACGGCCTTTGCCCCCCTCGATACCAACGGCAAAAATCTCAAGAAAGAGGTGGGCAAGATCCTGTGC ACTGACTGCGCCACCCGGCCCAAGTTGAAGAAGATGAAGAGCCAGACGGGACAGGTGGGTGAGAAGCAA TCGCTGAAGTGTGAGGCAGCAGCCGGTAATCCCCAGCCTTCCTACCGTTGGTTCAAGGATGGCAAGGAG CTCAACCGCAGCCGAGACATTCGCATCAAATATGGCAACGGCAGAAAGAACTCACGACTACAGTTCAAC AAGGTGAAGGTGGAGGACGCTGGGGAGTATGTCTGCGAGGCCGAGAACATCCTGGGGAAGGACACCGTC CGGGGCCGGCTTTACGTCAACAGCGTGAGCACCACCCTGTCATCCTGGTCGGGGCACGCCCGGAAGTGC AACGAGACAGCCAAGTCCTATTGCGTCAATGGAGGCGTCTGCTACTACATCGAGGGCATCAACCAGCTC TCCTGCAAGTGTCCTGTGGGATACACCGGGGACAGGTGTCAGCAGTTCGCAATGGTCAACTTCTCCAAA GCCGAGGAGCTGTACCAGAAGAGGGTCCTGACCATCACGGGCATCTGCGTGGCTCTGCTGGTCGTGGGC ATCGTCTGTGTGGTGGCCTACTGCAAGACCAAAAAACAGCGGAAGCAGATGCACAACCACCTCCGGCAG AACATGTGCCCGGCCCATCAGAACCGGAGCTTGGCCAATGGGCCCAGCCACCCCCGGCTGGACCCAGAG GAGATCCAGATGGCAGATTATATTTCCAAGAACGTGCCAGCCACAGACCATGTCATCAGGAGAGAAACT GAGACCACCTTCTCTGGGAGCCACTCCTGTTCTCCTTCTCACCACTGCTCCACAGCCACACCCACCTCC AGCCACAGACACGAGAGCCACACGTGGAGCCTGGAACGTTCTGAGAGCCTGACTTCTGACTCCCAGTCG GGGATCATGCTATCATCAGTGGGTACCAGCAAATGCAACAGCCCAGCATGTGTGGAGGCCCGGGCAAGG CGGGCAGCAGCCTACAACCTGGAGGAGCGGCGCAGGGCCACCGCGCCACCCTATCACGATTCCGTGGAC TCCCTTCGCGACTCCCCACACAGCGAGAGGTACGTGTCGGCCCTGACCACGCCCGCGCGCCTCTCGCCC GTGGACTTCCACTACTCGCTGGCCACGCAGGTGCCAACTTTCGAGATCACGTCCCCCAACTCGGCGCAC GCCGTGTCGCTGCCGCCGGCGGCGCCCATCAGTTACCGCCTGGCCGAGCAGCAGCCGTTACTGCGGCAC CCGGCGCCCCCCGGCCCGGGACCCGGACCCGGGCCCGGGCCCGGGCCCGGCGCAGACATGCAGCGCAGC TATGACAGCTACTATTACCCCGCGGCGGGGCCCGGACCGCGGCGCGGGACCTGCGCGCTCGGCGGCAGC CTGGGCAGCCTGCCTGCCAGCCCCTTCCGCATCCCCGAGGACGACGAGTACGAGACCACGCAGGAGTGC GCGCCCCCGCCGCCGCCGCGGCCGCGCGCGCGCGGTGCGTCCCGCAGGACGTCGGCGGGGCCCCGGCGC TGGCGCCGCTCGCGCCTCAACGGGCTGGCGGCGCAGCGCGCACGGGCGGCGAGGGACTCGCTGTCGCTG AGCAGCGGCTCGGGCGGCGGCTCAGCCTCGGCGTCGGACGACGACGCGGACGACGCGGACGGGGCGCTG GCGGCCGAGAGCACACCTTTCCTGGGCCTGCGTGGGGCGCACGACGCGCTGCGCTCGGACTCGCCGCCA CTGTGCCCGGCGGCCGACAGCAGGACTTACTACTCACTGGACAGCCACAGCACGCGGGCCAGCAGCAGA CACAGCCGCGGGCCGCCCCCGCGGGCCAAGCAGGACTCGGCGCCACTCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_013981
Insert Size	2535 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_013981.1
RefSeq Size	3002 bp
RefSeq ORF	2535 bp
Locus ID	9542
UniProt ID	O14511
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	ErbB signaling pathway
MW	90.9 kDa
Gene Summary	This gene encodes a novel member of the neuregulin family of growth and differentiation factors. Through interaction with the ERBB family of receptors, this protein induces the growth and differentiation of epithelial, neuronal, glial, and other types of cells. The gene consists of 12 exons and the genomic structure is similar to that of neuregulin 1, another member of the neuregulin family of ligands. The products of these genes mediate distinct biological processes by acting at different sites in tissues and eliciting different biological responses in cells. This gene is located close to the region for demyelinating Charcot-Marie-Tooth disease locus, but is not responsible for this disease. Alternative transcript variants encoding distinct isoforms have been described. [provided by RefSeq, May 2010] Transcript Variant: This variant (2) lacks an alternate in-frame exon but includes a different in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2, also known as beta or beta1) is shorter than isoform 1. This variant is supported by data in PMID:10369162. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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