Phospholipase C epsilon 1 (PLCE1) (NM_016341) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NPHS3; PLCE; PPLC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC308896 representing NM_016341. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACTTCTGAAGAAATGACAGCTTCTGTTCTCATACCTGTGACTCAGAGAAAAGTGGTTTCTGCCCAG TCGGCTGCAGATGAAAGTAGTGAAAAGGTCTCAGACATCAATATTTCAAAAGCACATACTGTCAGACGA AGTGGGGAGACTTCTCATACCATCTCACAACTGAACAAACTTAAAGAAGAACCTTCTGGAAGCAACTTG CCAAAGATTCTCTCAATAGCGAGGGAGAAAATAGTGAGTGATGAGAACAGTAATGAAAAATGTTGGGAG AAAATCATGCCAGATTCTGCGAAAAACCTTAACATTAACTGCAACAACATATTGAGAAACCATCAGCAT GGCCTTCCTCAGAGACAATTTTATGAAATGTACAACTCTGTTGCTGAGGAAGACTTGTGTTTAGAAACT GGAATTCCTTCTCCACTGGAAAGAAAGGTGTTCCCTGGAATTCAACTGGAACTAGACAGACCTTCCATG GGCATTAGTCCTTTAGGAAATCAGTCAGTGATCATAGAGACAGGCAGAGCACACCCTGACAGCAGAAGG GCAGTATTTCATTTTCATTATGAAGTTGACAGAAGAATGTCAGACACTTTCTGTACCCTATCAGAAAAC TTAATTTTAGACGATTGTGGAAATTGTGTACCACTACCTGGGGGTGAGGAGAAGCAAAAGAAAAACTAT GTGGCATATACCTGTAAACTGATGGAATTGGCCAAAAATTGTGATAATAAGAATGAGCAGCTGCAGTGT GATCATTGTGACACCTTGAATGATAAATACTTTTGCTTTGAAGGCTCTTGTGAGAAGGTTGACATGGTA TATTCAGGTGATAGCTTTTGTAGGAAAGACTTTACTGACAGTCAAGCTGCCAAGACCTTTTTGAGCCAT TTTGAGGACTTCCCTGATAATTGTGATGATGTAGAAGAAGACGCTTTTAAAAGCAAAAAGGAGCGATCC ACTTTGTTAGTCAGGAGATTCTGTAAAAATGACAGAGAAGTTAAGAAATCTGTGTATACTGGAACAAGA GCAATTGTGAGAACTCTGCCTTCTGGCCACATTGGGCTGACTGCATGGAGTTACATAGATCAGAAGAGA AATGGTCCCTTACTGCCTTGTGGGAGAGTAATGGAACCCCCGTCAACAGTGGAGATAAGGCAAGATGGG AGCCAACGTCTGTCAGAAGCCCAGTGGTATCCTATCTACAATGCAGTGAGAAGAGAAGAAACAGAAAAT ACAGTTGGATCTCTACTCCATTTCCTCACCAAGCTCCCAGCCTCCGAGACAGCCCATGGAAGGATAAGC GTTGGTCCATGCTTAAAGCAATGTGTCCGAGACACTGTATGTGAGTATCGCGCCACCCTCCAAAGGACT TCAATATCGCAGTACATCACCGGTTCTCTCCTAGAAGCAACCACGTCTTTGGGAGCAAGAAGTGGCCTT CTCAGTACTTTTGGAGGATCCACTGGACGAATGATGCTGAAAGAACGCCAGCCAGGCCCCTCTGTGGCC AATTCCAATGCCCTCCCTTCAAGTTCAGCTGGGATCAGCAAGGAGCTGATCGATCTGCAGCCTCTCATC CAGTTCCCAGAGGAAGTCGCCAGCATCCTGATGGAGCAAGAGCAGACTATTTACCGCAGGGTCTTGCCA GTCGACTACCTTTGCTTCTTAACACGGGACTTGGGCACTCCTGAATGCCAGAGCTCCTTGCCCTGCCTC AAAGCATCCATCTCAGCGTCGATTCTTACCACTCAGAATGGAGAGCACAATGCCCTTGAAGATCTGGTG ATGAGGTTTAATGAGGTGAGCTCCTGGGTGACATGGCTGATCCTCACGGCAGGCTCCATGGAGGAGAAG CGAGAAGTCTTTTCATATTTGGTGCATGTGGCCAAATGCTGCTGGAACATGGGCAACTACAACGCTGTC ATGGAGTTCTTGGCTGGCCTCAGGTCAAGAAAAGTTTTAAAAATGTGGCAGTTCATGGACCAGTCTGAT ATTGAGACCATGAGGAGCCTGAAGGATGCTATGGCCCAGCATGAGTCCTCTTGTGAGTACAGAAAGGTG GTGACACGTGCCCTGCACATCCCTGGCTGTAAGGTGGTTCCATTCTGTGGGGTGTTTCTGAAGGAGCTC TGTGAAGTGCTTGACGGCGCCTCCGGTCTCATGAAGCTTTGCCCGCGGTACAATTCCCAAGAAGAAACT TTAGAGTTTGTAGCAGATTACAGTGGACAAGATAATTTCTTACAACGAGTGGGACAAAATGGCTTAAAG AATTCGGAGAAGGAGTCCACTGTCAACAGCATCTTTCAGGTCATCCGGAGCTGCAATCGAAGTCTGGAG ACAGACGAGGAGGACAGCCCCAGTGAAGGAAACAGCTCCAGGAAAAGCTCCTTGAAGGATAAAAGCCGA TGGCAGTTTATAATTGGAGATTTGTTGGATTCAGACAATGACATCTTTGAGCAATCCAAAGAATACGAC TCTCATGGTTCAGAGGACTCACAGAAGGCCTTCGACCATGGGACGGAGCTCATCCCTTGGTACGTGCTG TCCATCCAAGCCGATGTGCACCAGTTCCTGCTGCAGGGGGCCACGGTCATCCACTACGACCAGGACACA CACCTCTCTGCCCGCTGCTTCCTCCAGCTTCAGCCCGACAATAGCACCTTGACCTGGGTAAAGCCCACA ACTGCCTCCCCAGCCAGCAGTAAAGCAAAACTTGGTGTACTTAATAACACAGCTGAGCCTGGAAAATTC CCACTACTGGGTAATGCTGGATTAAGTAGCCTGACGGAAGGGGTCTTGGATCTTTTTGCAGTGAAGGCT GTATACATGGGCCACCCTGGCATTGATATACACACTGTGTGTGTTCAGAACAAACTGGGTAGCATGTTC CTGTCAGAGACTGGTGTGACATTGCTCTATGGGCTTCAGACCACAGACAACAGATTATTGCACTTCGTG GCACCAAAGCACACAGCTAAAATGCTCTTCAGCGGATTATTGGAACTCACTAGAGCTGTGAGAAAGATG AGGAAATTCCCTGACCAAAGACAGCAGTGGCTGCGGAAACAGTACGTCAGCCTTTATCAGGAGGATGGA CGGTATGAAGGCCCAACTTTGGCTCACGCTGTGGAGTTGTTTGGTGGCAGACGGTGGAGTGCTCGAAAC CCCAGCCCCGGAACATCAGCAAAGAATGCTGAGAAGCCCAATATGCAGAGAAACAATACCCTGGGCATA AGCACTACCAAGAAAAAGAAGAAAATCCTCATGAGGGGTGAGAGTGGAGAGGTAACTGACGATGAGATG GCAACCCGAAAGGCCAAGATGCACAAAGAGTGTCGAAGCCGGAGTGGTTCTGATCCTCAAGACATTAAT GAACAAGAAGAATCAGAGGTGAATGCCATCGCTAACCCTCCAAACCCCCTCCCTTCCAGAAGAGCCCAC TCTTTGACCACAGCTGGGTCCCCCAACTTGGCTGCCGGGACGTCATCTCCCATCAGGCCAGTGTCCTCC CCTGTGCTGTCTTCTTCAAACAAGAGCCCATCCAGTGCTTGGAGCAGTAGTAGCTGGCACGGGCGGATC AAAGGCGGCATGAAGGGATTTCAGAGCTTCATGGTTTCAGATAGCAACATGAGTTTTGTTGAATTTGTT GAGCTGTTCAAATCATTCAGTGTCAGGAGCCGCAAGGACCTGAAGGATCTGTTTGATGTCTATGCAGTG CCCTGCAACCGATCTGGCTCCGAGTCAGCCCCACTCTACACCAACCTGACAATTGATGAAAACACCAGC GATCTTCAGCCTGACCTAGATCTGTTGACCAGAAATGTCTCGGATTTGGGGTTGTTCATTAAGAGTAAA CAGCAGCTATCGGACAACCAGAGGCAGATATCTGATGCCATTGCTGCTGCAAGCATTGTGACAAATGGC ACTGGGATTGAGAGCACATCTCTGGGCATTTTTGGGGTGGGCATACTTCAGCTCAACGATTTCCTCGTG AATTGCCAAGGAGAACACTGCACTTATGATGAAATCCTCAGCATCATCCAGAAGTTCGAGCCTAGCATC AGTATGTGTCATCAGGGACTAATGTCATTTGAAGGGTTTGCCAGGTTTCTGATGGATAAAGAAAATTTT GCCTCAAAAAATGATGAGTCACAGGAGAACATTAAAGAACTGCAGCTACCCCTCTCATACTATTACATC GAATCTTCGCACAATACCTACCTCACGGGCCATCAGCTCAAAGGAGAATCCTCGGTAGAACTCTACAGC CAGGTCCTTTTGCAAGGCTGTCGAAGTGTAGAATTGGACTGCTGGGACGGAGACGATGGGATGCCCATC ATTTATCATGGACATACGCTGACAACCAAGATCCCCTTCAAGGAAGTGGTTGAAGCCATTGATCGCAGT GCCTTCATCAACTCTGACCTGCCAATCATCATATCGATTGAGAACCACTGTTCATTGCCTCAGCAACGA AAAATGGCAGAAATTTTCAAGACTGTGTTTGGAGAAAAGCTGGTGACTAAATTCTTATTTGAGACTGAT TTCTCAGATGATCCAATGCTTCCTTCACCTGACCAACTCAGAAAGAAAGTTCTTCTTAAAAACAAGAAG CTAAAAGCCCATCAGACGCCAGTGGATATCTTAAAGCAAAAGGCTCATCAGTTAGCATCTATGCAAGTG CAGGCTTATAATGGTGGGAATGCCAACCCCCGACCTGCCAATAATGAGGAAGAGGAAGATGAGGAGGAC GAATATGATTATGACTATGAATCCCTTTCTGATGACAACATTCTGGAAGACAGACCTGAAAATAAATCA TGTAATGACAAGCTTCAGTTTGAATATAATGAAGAAATCCCAAAGAGGATAAAGAAAGCAGATAACTCT GCTTGCAACAAAGGAAAGGTTTATGATATGGAACTGGGAGAAGAATTTTATCTTGATCAGAATAAAAAG GAAAGCAGACAGATTGCACCAGAGCTTTCTGACCTTGTAATCTATTGTCAAGCAGTAAAATTTCCAGGA CTGTCAACTCTAAATGCATCTGGCTCTAGCAGAGGAAAAGAAAGGAAAAGCAGGAAGTCCATTTTTGGC AACAATCCGGGCAGAATGAGCCCAGGGGAGACAGCATCATTTAACAAAACATCTGGAAAAAGTTCCTGT GAAGGCATTCGACAGACCTGGGAGGAATCTTCTTCCCCTCTCAACCCAACCACGTCCCTCAGTGCTATC ATTAGAACTCCCAAATGTTATCATATCTCGTCGCTGAATGAAAATGCCGCCAAACGTCTGTGTCGCAGG TATTCTCAGAAACTGACCCAGCACACCGCCTGTCAGCTGCTGAGAACTTACCCTGCTGCCACCCGCATC GACTCTTCCAACCCGAACCCCCTCATGTTCTGGCTCCATGGGATACAGCTTGTGGCACTCAACTACCAG ACTGATGATCTCCCTTTACATTTAAATGCTGCAATGTTTGAGGCAAATGGTGGTTGTGGTTATGTATTG AAACCTCCAGTTCTGTGGGACAAGAACTGCCCCATGTATCAGAAGTTTTCTCCACTAGAAAGAGATCTG GACAGCATGGATCCTGCAGTCTATTCTTTAACTATTGTCTCTGGTCAGAATGTGTGCCCCAGTAATAGC ATGGGAAGCCCGTGCATTGAAGTCGACGTCCTGGGCATGCCTCTGGACAGCTGCCATTTCCGCACAAAG CCCATCCATCGAAACACCCTGAACCCCATGTGGAACGAGCAGTTTCTGTTCCACGTTCACTTCGAAGAT CTTGTATTTCTTCGTTTTGCAGTTGTGGAAAACAATAGTTCAGCGGTAACTGCTCAGAGAATCATTCCA CTGAAAGCTTTAAAACGAGGATATCGACATCTTCAGCTGCGAAACCTTCACAATGAAGTCTTGGAGATT TCTAGTTTATTCATTAACAGCAGAAGGATGGAAGAAAATTCCTCTGGCAATACCATGTCAGCCTCTTCG ATGTTTAATACAGAAGAAAGAAAATGTTTGCAGACTCACAGAGTCACGGTGCATGGGGTCCCAGGGCCA GAGCCCTTTACCGTTTTCACTATTAATGGAGGCACCAAGGCAAAGCAGCTTCTGCAGCAAATTCTGACA AATGAACAAGACATCAAACCTGTTACCACAGACTATTTTTTGATGGAAGAAAAATATTTTATATCTAAA GAAAAGAATGAATGTAGGAAACAACCATTCCAGAGAGCCATTGGTCCAGAAGAGGAGATCATGCAAATT TTAAGCAGCTGGTTTCCAGAAGAGGGATACATGGGCAGGATTGTCTTAAAAACCCAGCAGGAAAACCTA GAAGAGAAAAACATTGTTCAAGATGACAAAGAGGTGATCTTGAGCTCAGAGGAGGAGAGTTTCTTTGTC CAAGTGCATGATGTTTCTCCAGAGCAACCTCGAACAGTCATCAAAGCACCCCGCGTCAGCACTGCACAG GATGTCATTCAGCAGACCTTATGCAAAGCCAAATATTCCTACAGCATCCTGAGCAACCCCAATCCAAGC GACTATGTGCTTTTGGAAGAGGTGGTGAAAGACACTACCAACAAGAAGACTACCACACCAAAGTCCTCT CAGCGGGTCCTTCTGGATCAGGAGTGTGTGTTTCAAGCCCAAAGCAAGTGGAAAGGTGCAGGAAAATTC ATCCTTAAGCTAAAGGAGCAGGTGCAGGCATCTCGAGAAGATAAAAAGAAAGGCATTTCTTTCGCAAGT GAACTCAAGAAGCTCACCAAGTCAACTAAACAGCCCCGAGGACTTACATCACCTTCTCAGCTCTTGACC TCAGAAAGTATCCAAACCAAGGAGGAGAAACCTGTGGGTGGCTTGTCCTCCAGTGACACAATGGATTAC CGACAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_016341
Insert Size	6909 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_016341.3
RefSeq Size	7992 bp
RefSeq ORF	6909 bp
Locus ID	51196
UniProt ID	Q9P212
Domains	C2, RA, PI-PLC-X, PI-PLC-Y, RasGEF
Protein Families	Druggable Genome
Protein Pathways	Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
MW	258.7 kDa
Gene Summary	This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1; also known as PLCepsilon1a).

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