PARD3 (NM_019619) Human Untagged Clone

CAT#: SC308839

PARD3 (untagged)-Human par-3 partitioning defective 3 homolog (C. elegans) (PARD3), transcript variant 1



  "NM_019619" in other vectors (4)

CNY 26,030.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal anti-PARD3 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ASIP; Baz; PAR3; PAR3alpha; PARD-3; PARD3A; PPP1R118; SE2-5L16; SE2-5LT1; SE2-5T2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC308839 representing NM_019619.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAAAGTGACCGTGTGCTTCGGACGGACCCGGGTGGTCGTGCCGTGCGGGGACGGCCACATGAAAGTT
TTCAGCCTCATCCAGCAGGCGGTGACCCGCTACCGGAAGGCCATCGCCAAGGATCCAAACTACTGGATA
CAGGTGCATCGCTTGGAACATGGAGATGGAGGAATACTAGACCTTGATGACATTCTTTGTGATGTAGCA
GACGATAAAGACAGACTGGTAGCAGTGTTTGATGAGCAGGATCCACATCACGGAGGTGATGGCACCAGT
GCCAGTTCCACGGGTACCCAGAGCCCAGAGATATTTGGTAGTGAGCTTGGCACCAACAATGTCTCAGCC
TTTCAGCCTTACCAAGCAACAAGTGAAATTGAGGTCACACCTTCAGTCCTTCGAGCAAATATGCCTCTT
CATGTTCGACGCAGTAGTGACCCAGCTCTAATTGGCCTCTCCACTTCTGTCAGTGATAGTAATTTTTCC
TCTGAAGAGCCTTCAAGGAAAAATCCCACACGCTGGTCAACAACAGCTGGCTTCCTCAAGCAGAACACT
GCTGGGAGTCCTAAAACCTGCGACAGGAAGAAAGATGAAAACTACAGAAGCCTCCCGCGGGATACTAGT
AACTGGTCTAACCAATTTCAGAGAGACAATGCTCGCTCGTCTCTGAGTGCCAGTCACCCAATGGTGGGC
AAGTGGCTGGAGAAACAAGAACAGGATGAGGATGGGACAGAAGAGGATAACAGTCGTGTTGAACCTGTT
GGACATGCTGACACGGGTTTGGAGCATATACCCAACTTTTCTCTGGATGATATGGTAAAGCTCGTAGAA
GTCCCCAACGATGGAGGGCCTCTGGGAATCCATGTAGTGCCTTTCAGTGCTCGAGGCGGCAGAACCCTG
GGGTTATTAGTAAAACGATTGGAGAAAGGTGGTAAAGCTGAACATGAAAATCTTTTTCGTGAGAATGAT
TGCATTGTCAGGATTAATGATGGCGACCTTCGAAATAGAAGATTTGAACAAGCACAACATATGTTTCGC
CAAGCCATGCGTACACCCATCATTTGGTTCCATGTGGTTCCTGCAGCAAATAAAGAGCAGTATGAACAA
CTATCCCAAAGTGAGAAGAACAATTACTATTCAAGCCGTTTTAGCCCTGACAGCCAGTATATTGACAAC
AGGAGTGTGAACAGTGCAGGGCTTCACACGGTGCAGAGAGCACCCCGACTGAACCACCCGCCTGAGCAG
ATAGACTCTCACTCAAGACTACCTCATAGCGCACACCCCTCGGGAAAACCACCATCCGCTCCAGCCTCG
GCACCTCAGAATGTATTTAGTACGACTGTAAGCAGTGGTTATAACACCAAAAAAATAGGCAAGAGGCTT
AATATCCAGCTTAAGAAAGGTACAGAAGGTTTGGGATTCAGCATCACTTCCAGAGATGTAACAATAGGT
GGCTCAGCTCCAATCTATGTGAAAAACATTCTCCCCCGGGGGGCGGCCATTCAGGATGGCCGACTTAAG
GCAGGAGACAGACTTATAGAGGTAAATGGAGTAGATTTAGTGGGCAAATCCCAAGAGGAAGTTGTTTCG
CTGTTGAGAAGCACCAAGATGGAAGGAACTGTGAGCCTTCTGGTCTTTCGCCAGGAAGACGCCTTCCAC
CCAAGGGAACTGAATGCAGAGCCAAGCCAGATGCAGATTCCAAAAGAAACGAAAGCAGAAGATGAGGAT
ATTGTTCTTACACCTGATGGCACCAGGGAATTTCTGACATTTGAAGTCCCACTTAATGATTCAGGATCT
GCAGGCCTTGGTGTCAGTGTCAAAGGTAACCGGTCAAAAGAGAACCACGCAGATTTGGGAATCTTTGTC
AAGTCCATTATTAATGGAGGAGCAGCATCTAAAGATGGAAGGCTTCGGGTGAATGATCAACTGATAGCA
GTAAATGGAGAATCCCTGTTGGGCAAGACAAACCAAGATGCCATGGAAACCCTAAGAAGGTCTATGTCT
ACTGAAGGCAATAAACGAGGAATGATCCAGCTTATTGTTGCAAGGAGAATAAGCAAGTGCAATGAGCTG
AAGTCACCTGGGAGCCCCCCTGGACCTGAGCTGCCCATTGAAACAGCGTTGGATGATAGAGAACGAAGA
ATTTCCCATTCCCTCTACAGTGGGATTGAGGGGCTTGATGAATCGCCCAGCAGAAATGCTGCCCTCAGT
AGGATAATGGGTGAGTCAGGTAAATACCAGCTGTCCCCTACAGTGAATATGCCCCAAGATGACACTGTC
ATTATAGAAGATGACAGGTTGCCAGTGCTTCCTCCACATCTCTCTGACCAGTCCTCTTCCAGCTCCCAT
GATGATGTGGGGTTTGTGACGGCAGATGCTGGTACTTGGGCCAAGGCTGCAATCAGTGATTCAGCCGAC
TGCTCTTTGAGTCCAGATGTTGATCCAGTTCTTGCTTTTCAACGAGAAGGATTTGGACGTCAGAGTATG
TCAGAAAAACGCACAAAGCAATTTTCAGATGCCAGTCAATTGGATTTCGTTAAAACACGAAAATCAAAA
AGCATGGATTTAGGTATAGCTGACGAGACTAAACTCAATACAGTGGATGACCAGAAAGCAGGTTCTCCC
AGCAGAGATGTGGGTCCTTCCCTGGGTCTGAAGAAGTCAAGCTCGTTGGAGAGTCTGCAGACCGCAGTT
GCCGAGGTGACTTTGAATGGGGATATTCCTTTCCATCGTCCACGGCCGCGGATAATCAGAGGCAGGGGA
TGCAATGAGAGCTTCAGAGCTGCCATCGACAAATCTTATGATAAACCCGCGGTAGATGATGATGATGAA
GGCATGGAGACCTTGGAAGAAGACACAGAAGAAAGTTCAAGATCAGGGAGAGAGTCTGTATCCACAGCC
AGTGATCAGCCTTCCCACTCTCTGGAGAGACAAATGAATGGAAACCAAGAGAAAGGTGATAAGACTGAT
AGAAAAAAGGATAAAACTGGAAAAGAAAAGAAGAAAGATAGAGATAAGGAGAAGGATAAAATGAAAGCC
AAGAAGGGAATGCTGAAGGGCTTGGGAGACATGTTCAGGTTTGGCAAACATCGAAAAGATGACAAGATT
GAGAAAACGGGTAAAATAAAAATACAGGAATCCTTTACATCAGAAGAGGAGAGGATACGAATGAAGCAG
GAGCAGGAGAGGATTCAAGCCAAAACTCGAGAATTTAGGGAACGACAAGCTCGAGAGCGTGACTATGCT
GAAATTCAAGATTTTCATCGGACATTTGGCTGTGATGATGAGTTAATGTATGGGGGAGTTTCTTCTTAT
GAAGGTTCCATGGCTCTCAACGCTAGACCTCAGAGCCCACGAGAAGGGCATATGATGGATGCTTTGTAT
GCCCAAGTCAAGAAGCCGCGGAATTCCAAACCCTCACCTGTAGACAGTAACAGATCAACTCCTAGCAAT
CATGATCGGATACAGCGTCTGAGGCAAGAATTTCAGCAAGCAAAGCAAGATGAAGATGTAGAAGATCGT
CGGCGGACCTATAGTTTTGAGCAACCCTGGCCGAACGCACGGCCGGCGACGCAGAGCGGGCGACACTCG
GTGTCCGTGGAGGTGCAGATGCAGCGGCAGCGGCAGGAGGAGCGCGAGAGCTCCCAGCAGGCCCAGCGC
CAGTACAGCTCTCTGCCTCGGCAAAGCAGGAAAAATGCCAGCTCGGTCTCCCAGGACTCTTGGGAGCAG
AACTACTCCCCTGGGGAAGGCTTCCAGAGTGCCAAAGAGAACCCCAGGTACTCCAGCTACCAAGGCTCC
AGGAACGGCTACCTGGGAGGACATGGCTTCAACGCCAGGGTCATGCTGGAAACTCAGGAGCTCCTTCGC
CAGGAACAGAGGCGGAAGGAGCAGCAGATGAAGAAGCAGCCTCCTTCCGAGGGGCCCAGCAACTATGAC
TCGTATAAGAAAGTCCAGGACCCCAGTTACGCCCCTCCCAAGGGGCCCTTCCGGCAAGATGTGCCCCCC
TCCCCTTCTCAGGTTGCGAGGCTGAACAGACTTCAGACTCCTGAGAAAGGGAGGCCCTTCTATTCCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_019619
Insert Size 4071 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_019619.3
RefSeq Size 6013 bp
RefSeq ORF 4071 bp
Locus ID 56288
UniProt ID Q8TEW0
Domains PDZ
Protein Pathways Adherens junction, Chemokine signaling pathway, Endocytosis, Neuroactive ligand-receptor interaction, Tight junction
MW 151.4 kDa
Gene Summary This gene encodes a member of the PARD protein family. PARD family members interact with other PARD family members and other proteins; they affect asymmetrical cell division and direct polarized cell growth. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]
Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1). This variant has also been called 'variant a'. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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