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HSPC302 (TBCK) (NM_033115) Human Untagged Clone
CAT＃: SC308733
TBCK (untagged)-Human TBC1 domain containing kinase (TBCK), transcript variant 4
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HSPC302; IHPRF3; TBCKL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC308733 representing NM_033115.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTTCCCCTGAAGGACGCTGAAATGGGAGCCTTTACCTTCTTTGCCTCGGCTCTGCCACATGATGTT TGTGGAAGCAATGGACTTCCTCTCACACCAAATTCCATCAAAATTTTAGGGCGCTTTCAAATCCTTAAA ACCATCACCCATCCCAGACTCTGCCAGTATGTGGATATTTCTAGGGGAAAGCATGAACGACTAGTGGTC GTGGCTGAACATTGTGAACGTAGTCTGGAAGACTTGCTTCGAGAAAGGAAACCTGTGAGGTATCCCTCG TACTTGGCCCCTGAGGTAATTGCACAGGGAATTTTCAAAACCACTGATCACATGCCAAGTAAAAAACCA TTGCCTTCTGGCCCCAAATCAGATGTATGGTCTCTTGGAATCATTTTATTTGAGCTTTGTGTGGGAAGA AAATTATTTCAGAGCTTGGATATTTCTGAAAGACTAAAATTTTTGCTTACTTTGGATTGTGTAGATGAC ACTTTAATAGTTCTGGCTGAAGAGCATGGTTGTTTGGACATTATAAAGGAGCTTCCTGAAACTGTGATA GATCTTTTGAATAAGTGCCTTACCTTCCATCCTTCTAAGAGGCCAACCCCAGATGAATTAATGAAGGAC AAAGTATTCAGTGAGGTATCACCTTTATATACCCCCTTTACCAAACCTGCCAGTCTGTTTTCATCTTCT CTGAGATGTGCTGATTTAACTCTGCCTGAGGATATCAGTCAGTTGTGTAAAGATATAAATAATGATTAC CTGGCAGAAAGATCTATTGAAGAAGTGTATTACCTTTGGTGTTTGGCTGGAGGTGACTTGGAGAAAGAG CTTGTCAACAAGGAAATCATTCGATCCAAACCACCTATCTGCACACTCCCCAATTTTCTCTTTGAGGAT GGTGAAAGCTTTGGACAAGGTCGAGATAGAAGCTCGCTTTTAGATGATACCACTGTGACATTGTCGTTA TGCCAGCTAAGAAATAGATTGAAAGATGTTGGTGGAGAAGCATTTTACCCATTACTTGAAGATGACCAG TCTAATTTACCTCATTCAAACAGCAATAATGAGTTGTCTGCAGCTGCCACGCTCCCTTTAATCATCAGA GAGAAGGATACAGAGTACCAACTAAATAGAATTATTCTCTTCGACAGGCTGCTAAAGGCTTATCCATAT AAAAAAAACCAAATCTGGAAAGAAGCAAGAGTTGACATTCCTCCTCTTATGAGAGGTTTAACCTGGGCT GCTCTTCTGGGAGTTGAGGGAGCTATTCATGCCAAGTACGATGCAATTGATAAAGACACTCCAATTCCT ACAGATAGACAAATTGAAGTGGATATTCCTCGCTGTCATCAGTACGATGAACTGTTATCATCACCAGAA GGTCATGCAAAATTTAGGCGTGTATTAAAAGCCTGGGTAGTGTCTCATCCTGATCTTGTGTATTGGCAA GGTCTTGACTCACTTTGTGCTCCATTCCTATATCTAAACTTCAATAATGAAGCCTTGGCTTATGCATGT ATGTCTGCTTTTATTCCCAAATACCTGTATAACTTCTTCTTAAAAGACAACTCACATGTAATACAAGAG TATCTGACTGTCTTCTCTCAGATGATTGCATTTCATGATCCAGAGCTGAGTAATCATCTCAATGAGATT GGTTTCATTCCAGATCTCTATGCCATCCCTTGGTTTCTTACCATGTTTACTCATGTATTTCCACTACAC AAAATTTTCCACCTCTGGGATACCTTACTACTTGGGAATTCCTCTTTCCCATTCTGTATTGGAGTAGCA ATTCTTCAGCAGCTGCGGGACCGGCTTTTGGCTAATGGCTTTAATGAGTGTATTCTTCTCTTCTCCGAT TTACCAGAAATTGACATTGAACGCTGTGTGAGAGAATCTATCAACCTGTTTTGTTGGACTCCTAAAAGT GCTACTTACAGACAGCATGCTCAACCTCCAAAGCCATCTTCTGACAGCAGTGGAGGCAGAAGTTCGGCA CCTTATTTCTCTGCTGAGTGTCCAGATCCTCCAAAGACAGATCTGTCAAGAGAATCCATCCCATTAAAT GACCTGAAGTCAGAAGTATCACCACGGATTTCAGCAGAGGACCTGATTGACTTGTGTGAGCTCACAGTG ACAGGCCACTTCAAAACACCCAGCAAGAAAACAAAGTCCAGTAAACCAAAGCTCCTGGTGGTTGACATC CGGAATAGTGAAGACTTTATTCGTGGTCACATTTCAGGAAGCATCAACATTCCATTCAGTGCTGCCTTC ACTGCAGAAGGGGAGCTTACCCAGGGCCCTTACACTGCTATGCTCCAGAACTTCAAAGGGAAGGTCATT GTCATCGTGGGGCATGTGGCAAAACACACAGCTGAGTTTGCAGCTCACCTTGTGAAGATGAAATATCCA AGAATCTGTATTCTAGATGGTGGCATTAATAAAATAAAGCCAACAGGCCTCCTCACCATCCCATCTCCT CAAATATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_033115 |
| Insert Size | 2493 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033115.4 |
| RefSeq Size | 5120 bp |
| RefSeq ORF | 2493 bp |
| Locus ID | 93627 |
| UniProt ID | Q8TEA7 |
| Domains | TBC, pkinase, RHOD, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 93.7 kDa |
| Gene Summary | This gene encodes a protein that contains a protein kinase domain, a Rhodanase-like domain and the Tre-2/Bub2/Cdc16 (TBC) domain. The encoded protein is thought to play a role in actin organization, cell growth and cell proliferation by regulating the mammalian target of the rapamycin (mTOR) signaling pathway. This protein may also be involved in the transcriptional regulation of the components of the mTOR complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (4) lacks two in-frame exons in the 5' coding region compared to variant 1. This results in a shorter protein (isoform c) compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203605 | TBCK (Myc-DDK-tagged)-Human TBC1 domain containing kinase (TBCK), transcript variant 4 |
CNY 6,080.00 |
|
| RC203605L1 | Lenti-ORF clone of TBCK (Myc-DDK-tagged)-Human TBC1 domain containing kinase (TBCK), transcript variant 4 |
CNY 8,480.00 |
|
| RC203605L2 | Lenti-ORF clone of TBCK (mGFP-tagged)-Human TBC1 domain containing kinase (TBCK), transcript variant 4 |
CNY 5,890.00 |
|
| RC203605L3 | Lenti-ORF clone of TBCK (Myc-DDK-tagged)-Human TBC1 domain containing kinase (TBCK), transcript variant 4 |
CNY 5,890.00 |
|
| RC203605L4 | Lenti-ORF clone of TBCK (mGFP-tagged)-Human TBC1 domain containing kinase (TBCK), transcript variant 4 |
CNY 5,890.00 |
|
| RG203605 | TBCK (tGFP-tagged) - Human TBC1 domain containing kinase (TBCK), transcript variant 4 |
CNY 4,370.00 |
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