SPAG17 (NM_206996) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CT143; PF6; SPGF55
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC308355 representing NM_206996. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCACCCAAGAAGGAGAAAGGAGGAACTGTGAACACCAGTTCTAAGATATGGGAACCCTCGCTCATA GCTGCACAGTTCAATCAGAACGATTGGCAGGCCTCCATTGCTTTTGTGGTTGGGAACCAGATTGAAGAT GATCTTCTCATCCAAGCCCTTACCGTGGCTGTCCAGGTCCCTCAGCGTAAACTCTTCAGTATGGTGTCG TGGCAAGACATTCTCCAGCAGATTAATGAAATAAATACACTTGTTGGATCTGCTTCATCTAAAAAGGCA AAAAAACCTGTAGGTGGTAATGCTCCTTTATATTATGAGGTGTTAACGGCAGCAAAAGCAATTATGGAT AGTGGAGAGAAATTAACCTTACCACTGATAGGGAAACTCTTGAAATTTCAACTTCTCCAGATTAAATTT AAGGACCAACAGCGACGGGAAAATGAAAAGAAGGTAATAGAAGACAAACCTAAGTTAGAAAAGGATAAA GGGAAAGCAAAATCTCCCAAGGAGAAAAAGGCTCCAAGTGCCAAGCCTGCCAAAGGAAAGGGAAAGGAT CAGCCTGAGGCAAATGCACCAGTGAAAAAGACCACCCAGTTAAAGCGGAGAGGAGAAGACGACCACACC AATCGTTACATTGACGATGAGCCAGATGATGGTGCCCAACATTACATTATAGTTGTGGGCTTTAACAAT CCTCAGCTATTAGCAATTATGGCTGAGCTTGGCATTCCTATAACCAGCGTGATTAAAATATCTTCAGAG AATTATGAACCTCTGCAGACACACCTGGCAGCAGTTAACCAGCAGCAGGAAGTTCTTCTTCAGTCAGAA GATCTAGAAGCAGAAAAATTGAAGAAAGAAAATGCCATAAAAGAGCTTAAAACTTTCTGGAAGTACTTG GAACCAGTCCTGAATAATGAGAAACCTGAAACAAATCTCTTTGATGTTGCTCGACTTGAGTACATGGTC AAAGCAGCTGATTTTCCTTCTGACTGGTCAGATGGTGAGATGATGCTGAAATTGGGCACTGATATTTTT GAAAATATTGCCTGCTTGATGTATGACATCCTGGATTGGAAAAGGCAGCACCAGCACTATTTGGAAAGC ATGCAGCTTATTAATGTTCCACAAGTGGTTAATGAGAAACCTGTATTAGAAGCCATGCCAACTTCAGAG GCTCCACAACCTGCTGTACCAGCTCCTGGAAAGAAGAAAGCACAGTATGAAGAACCGCAAGCTCCACCA CCAGTGACTTCAGTCATCACAACTGAAGTAGACATGAGATATTACAATTATTTGCTGAATCCAATTCGA GAGGAATTCATTTCTGTGCCCCTGATACTGCATTGTATGCTGGAACAGGTTGTTGCAACTGAAGAAGAT CTCGTCCCACCCAGTCTGCGGGAGCCATCCCCCAGAGCAGACGGGCTAGACCACAGAATCGCAGCTCAC ATTGTGTCCCTTCTGCCCTCACTCTGTCTCTCAGAGAGGGAGAAAAAGAATCTTCATGACATATTTTTA TCTGAAGAAGAAAATGAAAGCAAAGCAGTGCCCAAAGGCCCCCTCCTACTGAACTATCATGATGCACAC GCCCACAAGAAGTACGCACTACAGGACCAAAAGAATTTTGATCCAGTTCAAATTGAGCAGGAGATGCAG TCCAAGTTGCCACTGTGGGAATTTCTTCAATTCCCTCTACCCCCACCATGGAACAACACTAAACGTCTA GCTACAATTCATGAGCTTATGCACTTTTGTACGAGTGATGTCTTGAGCTGGAATGAAGTAGAACGAGCC TTCAAGGTGTTTACTTTTGAGAGCCTGAAGCTCTCTGAGGTTGATGAAAAAGGGAAACTGAAACCTTCT GGGATGATGTGTGGGTCAGATTCTGAAATGTTCAACATACCGTGGGACAACCCTGCCAGATTTGCTAAA CAGATAAGGCAGCAATATGTCATGAAAATGAATACTCAAGAGGCCAAGCAGAAAGCAGATATTAAAATC AAAGACAGAACACTATTTGTGGATCAGAATTTGTCAATGTCTGTGCAAGATAATGAAAGCAACCGAGAA CCTTCAGATCCTAGTCAGTGTGATGCTAACAATATGAAGCATTCTGACTTGAATAATCTCAAACTCTCA GTCCCTGATAATAGACAGCTGTTAGAGCAGGAGAGCATCATGAAGGCTCAGCCCCAACATGAGTCTCTG GAGCAGACCACAAACAATGAGATCAAAGATGATGCAGTCACAAAGGCTGATTCTCATGAAAAGAAACCC AAGAAGATGATGGTGGAAGCAGATTTAGAGGACATAAAGAAAACACAGCAGCGCAGTCTAATGGACTGG AGTTTTACTGAACATTTTAAACCGAAAGTACTGCTTCAGGTCCTTCAAGAAGCCCATAAGCAATATAGG TGTGTTGATTCTTACTACCACACCCAAGACAACTCTTTACTTTTAGTCTTTCACAATCCAATGAATAGA CAACGTTTGCATTGTGAATATTGGAACATTGCTCTCCACTCCAATGTTGGATTCAGGAATTATTTGGAA CTTGTTGCAAAATCTATTCAAGATTGGATTACAAAAGAAGAAGCTATATATCAGGAATCTAAAATGAAT GAGAAAATCATCAGGACCAGAGCTGAGCTGGAATTGAAATCTTCTGCTAATGCCAAACTTACTTCTGCT AGCAAAATTTTTTCCATTAAAGAATCTAAAAGTAACAAAGGAATCAGCAAAACAGAGATATCAGATCAA GAAAAAGAAAAAGAGAAGGAAAAGATTCCTTTCATTTTAGAAGGCTCTCTCAAGGCATGGAAAGAAGAG CAACATCGATTAGCAGAAGAGGAGCGCTTAAGGGAAGAAAAGAAAGCAGAGAAGAAGGGTAAAGAAGCT GGTAAAAAGAAAGGCAAGGATAACGCAGAGAAAGAGGATAGTAGGTCTTTGAAGAAAAAATCACCTTAC AAGGAGAAATCTAAAGAAGAACAAGTCAAGATCCAAGAAGTAACAGAAGAGTCCCCCCACCAACCAGAA CCTAAGATAACTTACCCGTTTCACGGATACAATATGGGAAATATACCCACTCAAATCTCAGGGTCAAAT TACTACCTGTATCCTTCTGATGGGGGGCAGATTGAAGTGGAAAAGACAATGTTTGAAAAAGGCCCAACT TTTATCAAAGTGAGAGTGGTAAAGGACAACCACAATTTTATGATTCATTTAAATGACCCTAAGGAAATT GTGAAAAAGGAAGAGAAAGGGGATTATTATTTAGAAGAGGAAGAAGAAGGAGATGAGGAACAAAGTCTT GAAACGGAAGTATCAGATGCAAAGAATAAAGCTTTCAGCAAGTTTGGATCTTTTTCTGCCACCTTAGAA AATGGAATCTGCCTCTCGATAAGTTACTATGGATCAAATGGAATGGCACCAGAAGATAAGGATCCTGAT TTAGAAACAATATTGAATATCCCTTCAGCACTCACTCCAACAGTGGTTCCTGTTATAGTGACCGTTCCT CAAAGCAAAGCTAAAGGGAAAATAAAAGGCAAAGAAAAACCCAAAGAATCCCTTAAAGAAGAAGAACAC CCAAAAGAAGAAGAGAAAAAGGAAGAAGAAGTAGAACCAGAACCTGTTTTACAAGAGACTTTGGATGTT CCCACCTTCCAGAGCCTAAATGTGTCTTGCCCCAGTGGGCTCCTGTTGACTTTCATTGGACAAGAATCT ACAGGTCAATATGTTATAGATGAGGAACCCACCTGGGACATCATGGTCCGTCAGAGCTACCCCCAGAGG GTGAAGCACTATGAGTTCTATAAAACGGTGATGCCACCCGCAGAGCAGGAGGCTTCAAGGGTTATCACC AGTCAAGGCACTGTTGTCAAATATATGTTGGATGGATCCACACAGATTCTCTTTGCAGATGGTGCTGTG AGCAGGAGTCCCAATTCAGGTCTTATTTGTCCTCCTTCTGAAATGCCAGCAACGCCTCACAGTGGAGAT TTGATGGACTCTATTTCTCAGCAGAAATCAGAAACGATACCATCTGAGATTACCAACACAAAGAAAGGA AAAAGTCACAAAAGTCAGTCATCAATGGCCCATAAGGGTGAAATCCATGACCCTCCTCCAGAGGCAGTT CAAACTGTAACTCCTGTGGAGGTTCACATAGGCACCTGGTTTACAACCACACCTGAAGGAAATCGGATC GGCACCAAAGGATTAGAAAGAATAGCAGACTTGACCCCATTGTTATCCTTTCAGGCCACAGATCCTGTC AATGGAACGGTTATGACAACTCGAGAAGACAAAGTTGTCATAGTTGAAAGGAAAGATGGTACTCGGATA GTGGATCATGCTGATGGTACCAGAATCACAACCTTTTATCAAGTTTATGAAGATCAAATTATTCTGCCA GATGATCAAGAAACAACCGAGGGTCCTCGGACTGTCACCAGGCAGGTGAAGTGTATGCGGGTAGAAAGC TCACGCTATGCCACTGTTATCGCCAACTGTGAGGACAGTAGCTGCTGTGCCACCTTTGGAGATGGAACA ACTATTATTGCAAAGCCACAGGGAACATACCAGGTGTTACCTCCAAACACAGGCTCTCTTTATATTGAC AAGGATTGTTCAGCTGTGTACTGCCATGAGTCAAGCAGTAATATATACTATCCTTTTCAAAAGCGTGAG CAGCTGCGAGCTGGCAGGTACATCATGAGGCATACTTCAGAGGTTATCTGTGAGGTTCTGGATCCTGAG GGAAACACTTTTCAGGTCATGGCTGATGGTAGCATATCAACTATATTACCTGAAAAAAAATTGGAAGAT GATTTAAATGAGAAAACTGAGGGCTATGATAGTCTGTCCTCTATGCACCTTGAAAAGAATCATCAGCAA ATCTATGGTGAACATGTCCCCAGGTTTTTTGTTATGTATGCTGATGGATCAGGAATGGAACTTCTTCGA GACAGTGACATAGAAGAATATCTATCTTTGGCATATAAAGAATCAAATACTGTTGTTCTCCAAGAGCCA GTGCAGGAACAGCCAGGCACCCTAACCATCACAGTCCTTCGCCCTTTCCATGAAGCATCACCATGGCAA GTAAAAAAGGAAGATACAATTGTCCCTCCTAATCTCCGGTCAAGGTCATGGGAAACATTTCCCTCAGTT GAGAAAAAAACTCCAGGACCTCCGTTTGGTACTCAGATTTGGAAAGGCCTTTGCATTGAGTCCAAACAG CTAGTGAGTGCCCCGGGTGCCATACTCAAGAGCCCCAGTGTGCTACAGATGCGCCAATTCATTCAGCAT GAGGTCATAAAGAATGAGGTGAAACTGAGGCTGCAGGTTTCCCTTAAGGATTACATAAACTATATTCTA AAGAAAGAAGATGAGCTGCAGGAAATGATGGTTAAAGATTCCAGAACTGAGGAGGAGAGAGGCAATGCT GCTGATCTCCTCAAGCTGGTTATGTCTTTCCCTAAAATGGAGGAAACTACAAAAAGTCATGTTACTGAA GTTGCAGCTCACCTAACTGATTTATTCAAGCAGTCTTTGGCTACGCCTCCAAAATGCCCACCAGACACA TTTGGTAAAGATTTCTTTGAAAAGACATGGAGACACACAGCATCCTCAAAACGCTGGAAAGAAAAGATA GACAAAACGAGGAAGGAAATTGAGACAACACAGAATTACCTAATGGATATTAAGAACCGCATAATACCA CCCTTTTTTAAATCTGAATTGAACCAGTTATATCAGTCTCAGTATAATCACCTGGACAGTCTTTCCAAA AAACTGCCTTCTTTTACAAAGAAAAATGAAGATGCAAACGAAACAGCTGTTCAAGATACATCTGATCTT AATCTAGATTTCAAGCCACATAAGGTTTCAGAACAGAAATCCTCAAGTGTGCCTAGTCTTCCAAAACCA GAGATTTCTGCAGATAAGAAGGATTTCACTGCTCAGAACCAAACTGAAAATTTAACAAAATCTCCTGAA GAAGCAGAATCTTATGAGCCCGTGAAAATTCCAACCCAGTCCTTGCTGCAGGATGTTGCGGGACAAACA AGAAAAGAAAAAGTGAAGTTGCCTCATTATTTGCTGAGTTCCAAGCCTAAGTCTCAACCTCTTGCAAAG GTGCAAGATTCTGTTGGAGGAAAAGTGAACACATCCTCTGTTGCATCTGCTGCCATTAATAATGCAAAG TCATCCCTTTTTGGGTTCCATCTTCTCCCATCATCAGTCAAGTTTGGAGTGCTTAAGGAAGGACATACC TATGCCACAGTTGTAAAGCTCAAGAATGTTGGAGTGGACTTCTGCAGGTTTAAAGTAAAGCAGCCCCCA CCCAGCACAGGACTGAAAGTGACTTACAAACCTGGACCTGTGGCAGCTGGTATGCAGACAGAACTGAAT ATAGAGTTATTTGCCACAGCTGTTGGAGAGGATGGGGCCAAGGGATCAGCACACATCTCTCACAATATC GAGATTATGACAGAGCATGAGGTTCTGTTCCTACCTGTGGAAGCAACTGTTTTAACAAGCAGCAATTAT GATAAACGACCAAAAGACTTTCCCCAGGGAAAAGAAAATCCAATGGTCCAGAGAACTTCTACAATTTAT TCCTCCACACTTGGAGTCTTCATGTCTCGTAAAGTTTCTCCACATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_206996
Insert Size	6672 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_206996.2
RefSeq Size	7125 bp
RefSeq ORF	6672 bp
Locus ID	200162
UniProt ID	Q6Q759
MW	251.7 kDa
Gene Summary	This gene encodes a central pair protein present in the axonemes of cells with a "9 + 2" organization of microtubules. The encoded protein is required for the proper function of the axoneme. Mutations in the orthologous gene in mice lead to primary ciliary dyskinesia characterized by immotile nasal and tracheal cilia, reduced clearance of nasal mucus, profound respiratory distress, hydrocephalus, and neonatal lethality within twelve hours of birth due to impaired airway mucociliary clearance. Single-nucleotide polymorphisms in this gene are associated with human height and targeted mutations lead to skeletal malformations affecting the limbs in mice, suggesting a role for this gene in skeletal development. [provided by RefSeq, Feb 2017]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.