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MITF (NM_198159) Human Untagged Clone
CAT＃: SC307638
MITF (untagged)-Human microphthalmia-associated transcription factor (MITF), transcript variant 1
CNY 5,816.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bHLHe32; CMM8; COMMAD; MI; WS2; WS2A |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_198159 edited
TTCTCACTTTCCAGCAGTGGAAGGACGGGAAGCGGGAGCCATGCAGTCCGAATCGGGGAT CGTGCCGGATTTCGAAGTCGGGGAGGAGTTTCATGAAGAGCCCAAAACCTATTACGAACT CAAAAGTCAACCGCTGAAGAGCAGCAGTTCCGCCGAGCATCCTGGGGCCTCCAAGCCTCC GATAAGCTCCTCCAGTATGACATCACGCATCTTGCTACGCCAGCAACTCATGCGTGAGCA GATGCAGGAGCAGGAGCGCAGGGAGCAGCAGCAGAAGCTGCAGGCGGCCCAGTTCATGCA ACAGAGAGTGCCCGTGAGTCAGACACCAGCCATAAACGTCAGTGTGCCCACCACCCTTCC CTCTGCCACGCAGGTGCCGATGGAAGTCCTTAAGGTGCAGACCCACCTCGAAAACCCCAC CAAGTACCACATACAGCAAGCCCAACGGCAGCAGGTAAAGCAGTACCTTTCTACCACTTT AGCAAATAAACATGCCAACCAAGTCCTGAGCTTGCCATGTCCAAACCAGCCTGGCGATCA TGTCATGCCACCGGTGCCGGGGAGCAGCGCACCCAACAGCCCCATGGCTATGCTTACGCT TAACTCCAACTGTGAAAAAGAGGGATTTTATAAGTTTGAAGAGCAAAACAGGGCAGAGAG CGAGTGCCCAGGCATGAACACACATTCACGAGCGTCCTGTATGCAGATGGATGATGTAAT CGATGACATCATTAGCCTAGAATCAAGTTATAATGAGGAAATCTTGGGCTTGATGGATCC TGCTTTGCAAATGGCAAATACGTTGCCTGTCTCGGGAAACTTGATTGATCTTTATGGAAA CCAAGGTCTGCCCCCACCAGGCCTCACCATCAGCAACTCCTGTCCAGCCAACCTTCCCAA CATAAAAAGGGAGCTCACAGAGTCTGAAGCAAGAGCACTGGCCAAAGAGAGGCAGAAAAA GGACAATCACAACCTGATTGAACGAAGAAGAAGATTTAACATAAATGACCGCATTAAAGA ACTAGGTACTTTGATTCCCAAGTCAAATGATCCAGACATGCGCTGGAACAAGGGAACCAT CTTAAAAGCATCCGTGGACTATATCCGAAAGTTGCAACGAGAACAGCAACGCGCAAAAGA ACTTGAAAACCGACAGAAGAAACTGGAGCACGCCAACCGGCATTTGTTGCTCAGAATACA GGAACTTGAAATGCAGGCTCGAGCTCATGGACTTTCCCTTATTCCATCCACGGGTCTCTG CTCTCCAGATTTGGTGAATCGGATCATCAAGCAAGAACCCGTTCTTGAGAACTGCAGCCA AGACCTCCTTCAGCATCATGCAGACCTAACCTGTACAACAACTCTCGATCTCACGGATGG CACCATCACCTTCAACAACAACCTCGGAACTGGGACTGAGGCCAACCAAGCCTATAGTGT CCCCACAAAAATGGGATCCAAACTGGAAGACATCCTGATGGACGACACCCTTTCTCCCGT CGGTGTCACTGATCCACTCCTTTCCTCAGTGTCCCCCGGAGCTTCCAAAACAAGCAGCCG GAGGAGCAGTATGAGCATGGAAGAGACGGAGCACACTTGTTAGCGAATCCTCCCTGCACT GCATTCGCACAAACTGCTTCCTTTCTTGATT >OriGene 5' read for NM_198159 unedited
TGATTTGTATACGACTCCTATAGGGCGGCCGCGTATCCTCGCCCTTCTCACTTTCCAGCA GTGGAAGGACGGGAAGCGGGAGCCATGCAGTCCGAATCGGGGATTCGTGCCGGATTTCGA AGTCGGGGAGGAGTTTCATGAAGAGCCCAAAACCTATTACGAACTCAAAAGTCAACCGCT GAAGAGCAGCAGTTCCGCCGAGCATCCTGGGGCCTCCAAGCCTCCGATAAGCTCCTCCAG TATGACATCACGCATCTTGCTACGCCAGCAACTCATGCGTGAGCAGATGCAGGAGCAGGA GCGCAGGGAGCAGCAGCAGAAGCTGCAGGCGGCCCAGTTCATGCAACAGAGAGTGCCCGT GAGTCAGACACCAGCCATAAACGTCAGTGTGCCCACCACCCTTCCCTCTGCCACGCAGGT GCCGATGGAAGTCCTTAAGGTGCAGACCCACCTCGAAAACCCCACCAAGTACCACATACA GCAAGCCCAACGGCAGCAGGTAAAGCAGTACCTTTCTACCACTTTAGCAAATAAACATGC CAACCAAGTCCTGAGCTTGCCATGTCCAAACCAGCCTGGCGATCATGTCATGCCACCGGT GCCGGGGAGCAGCGCACCCAACAGCCCCATGGCTATGCTTACGCTTAACTCCAACTGTGA AAAAGAGGGATTTTATAAGTTTGAAGAGCAAAACAGGGCAGAGAGCGAGTGCCCAGGCAT GAACACACATTCACGAGCGTCCTGTATGCAGATGGATGATGTAATCGATGACATCATTAG CCTAGAATCAAGTTATAATGAGGAAATCTTGGCTTGATGGATCCTGCTTTGCAAATGGCA AATACGTTGCCTGTCTCGGGAAACTTGATTGATCTTTATGGAAACCAAGTCTGCCCCCAC CAGCCTCACCATCAGCAACTCCTGTCCAGCCACCTTCCCAACATAAAAAGGGAGCTCAAC AGAAGTTCTGAGCA |
| Restriction Sites | Please inquire |
| ACCN | NM_198159 |
| Insert Size | 1700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_198159.1, NP_937802.1 |
| RefSeq Size | 4788 bp |
| RefSeq ORF | 1563 bp |
| Locus ID | 4286 |
| UniProt ID | O75030 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Melanogenesis, Melanoma, Pathways in cancer |
| Gene Summary | The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017] Transcript Variant: This variant (1) represents the longest transcript, and encodes the longest isoform (1), also known as isoform MITF-A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Transcriptional and structural control of cell identity genes
,Fan, ZP;,
Thesis
[MITF]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211518 | MITF (Myc-DDK-tagged)-Human microphthalmia-associated transcription factor (MITF), transcript variant 1 |
CNY 5,808.00 |
|
| RC211518L1 | Lenti ORF clone of Human microphthalmia-associated transcription factor (MITF), transcript variant 1, Myc-DDK-tagged |
CNY 8,208.00 |
|
| RC211518L2 | Lenti ORF clone of Human microphthalmia-associated transcription factor (MITF), transcript variant 1, mGFP tagged |
CNY 8,208.00 |
|
| RC211518L3 | Lenti ORF clone of Human microphthalmia-associated transcription factor (MITF), transcript variant 1, Myc-DDK-tagged |
CNY 8,208.00 |
|
| RC211518L4 | Lenti ORF clone of Human microphthalmia-associated transcription factor (MITF), transcript variant 1, mGFP tagged |
CNY 8,208.00 |
|
| RG211518 | MITF (tGFP-tagged) - Human microphthalmia-associated transcription factor (MITF), transcript variant 1 |
CNY 7,408.00 |
