GARNL1 (RALGAPA1) (NM_194301) Human Untagged Clone

CAT#: SC307589

RALGAPA1 (untagged)-Human Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant 2



  "NM_194301" in other vectors (2)

CNY 40,090.00


货期*
11周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


RALGAPA1 rabbit polyclonal antibody
    • 25 ul

CNY 800.00
CNY 1,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1; TULIP1
Vector pCMV6 series
Sequence Data
>NCBI ORF sequence for NM_194301, the custom clone sequence may differ by one or more nucleotides
ATGTTCTCCAAGAAGCCGCACGGGGACGTGAAGAAGTCCACCCAGAAGGTGCTAGACACC
AAGAAGGACGCACTGACTCGCCTCAAGCACCTGCGCATCGTCATCGAGAATGCAGAATCT
ATTGATCTTAAACAGTTTTTCGACCAACATTTTTCACATATATACTATGTGTTCTTTGAA
AATTTTGTGACTATTGAAGCTAGTCTTAAACAGAAAGGTCACAAGTCTCAAAGAGAGGAA
TTGGATGCTATACTTTTTATTTTTGAGAAAATTTTACAACTTCTTCCAGAAAGAATTCAT
CAGCGATGGCAGTTTCATAGTATTGGATTGATTTTAAAGAAGCTACTTCACACAGGAAAC
TCCTTAAAGATTAGGCGGGAAGGTGTTCGTCTTTTCTTACTATGGTTGCAAGCTCTTCAG
AATAACTGTAGCAAAGAACAGCTCTGGATGTTTTCATGCTTAATCCCTGGATTTTCAGCA
CCACAGTCTGAACATGGACCTCGAACTTTAGATAATCTCATTAATCCTCCACTCAACCTT
CAAGAAACTCAAGTCACTATAGAAGAAATCACTCCTCTTGTCCCCCCACAATCAGGAGAT
AAAGGGCAAGAAGATCTCACAAGCTATTTTCTTGAAGCACTTCTAAAATACATAGTCATT
CAGGTAAAAAGTTTAGAATGGAAGAACAAAGAAAACCAAGAAAGGGGATTTTCATTTTTG
TTTTCACATTTTAAGAAATATTACTTGCCTTATATTTTTCCAAACATCTGTAAGGAAAAC
AGTTTATATCATCCTATACTTGACATCCCGCAGATGAGACCAAAGCCACATTATGTCGTG
ATAAAGAAAGATGCTGAAACCAATGAAGCAATCTATTGTACAAAGGAGCCTTTCATTAAG
GCTCGTGTTATTGTCATTCGTTGGCTGGTTTCTTTCTGGCTGGAGCCAAAACCACATACA
GGACCTCATATTCCTGGGATGGAAGGTGAAGTCTTGCCAAAGAATATTCAGAGAGCAGCT
GCTAGTTTAGTATCCAGAGAAGAAAGCAAAAATGATAATGCTGATAAAACAGACAGAACT
ACAGAACCCGAACAGTCTCATTCCAATACAAGCACTCTCACGGAGCGAGAACCTAGCTCA
TCTAGTCTCTGTAGTATTGATGAAGAACATCTCACAGACATTGAAATAGTTCGCAGAGTT
TTTTCTTCTAAAAGGAGTAATGTAAACTTTGTGACAGAGATATTTCGTCAGGCATTTTTA
TTACCAATTTGTGAAGCAGCAGCTATGAGAAAAGTGGTAAAAGTATATCAAGAATGGATC
CAACAAGAGGAAAAACCTTTGTTCATGCAAGAGCCTGAAGAAATTGTGATCACTTCTTCA
GACCTCCCTTGCATTGAAAATGTCACAGACCATGATATTTCAATGGAAGAAGGAGAAAAA
AGAGAAGAGGAAAATGGGACCAATACTGCTGATCATGTTCGAAATTCCAGTTGGGCAAAA
AACGGCTCCTACCAAGGTGCTCTTCATAACGCCTCTGAAGAAGCCACAGAACAAAACATA
CGAGCTGGTACCCAGGCAGTTTTGCAGGTGTTTATTATAAACTCATCAAATATATTTCTT
CTTGAACCTGCAAATGAAATAAAAAATCTTCTGGATGAACACACAGATATGTGTAAACGC
ATTCTTAACATTTATCGGTACATGGTTGTACAAGTATCAATGGACAAAAAGACTTGGGAA
CAGATGCTGCTTGTGTTGCTCAGAGTCACGGAATCTGTACTGAAGATGCCATCACAAGCT
TTTCTACAGTTCCAAGGGAAAAAAAATATGACCTTGGCAGGTCGACTTGCAGGACCACTT
TTCCAGACCCTTATAGTTGCCTGGATCAAAGCAAACCTAAATGTGTACATCTCCCGAGAA
CTTTGGGATGACTTACTGTCAGTATTGTCATCGCTGACCTATTGGGAAGAGTTGGCCACT
GAGTGGTCACTGACTATGGAGACATTAACTAAAGTTTTAGCTAGGAATTTATATAGTTTG
GATCTCAGTGATTTACCATTGGATAAGCTGAGTGAACAGAAACAAAAAAAGCACAAAGGG
AAAGGAGTTGGACATGAATTTCAGAAAGTTTCAGTTGACAAGTCATTTTCTAGAGGATGG
AGTCGTGATCAGCCTGGCCAAGCCCCAATGAGACAGAGGAGTGCAACAACCACTGGTTCT
CCAGGAACCGAAAAGGCGAGGAGTATAGTACGGCAAAAAACTGTCGATATTGATGATGCT
CAAATACTTCCCCGCTCAACTAGAGTCAGACATTTTTCACAAAGTGAAGAAACTGGAAAT
GAAGTTTTTGGTGCTTTGAATGAGGAGCAGCCATTGCCTCGAAGTAGCAGCACTTCTGAC
ATCTTGGAACCATTCACTGTTGAACGAGCCAAAGTCAATAAAGAGGACATGAGCCAAAAA
CTGCCTCCTCTTAATAGTGATATTGGCGGCAGCAGTGCTAATGTTCCTGATCTGATGGAT
GAGTTTATAGCAGAACGACTTCGAAGTGGTAATGCCTCGACTATGACAAGAAGAGGAAGT
AGTCCAGGCAGCCTGGAAATTCCCAAAGACCTCCCTGATATTCTAAACAAGCAGAACCAG
ATGCGCCCTATTGATGACCCAGGTGTGCCCTCAGAATGGACTTCTCCTGCCAGTGCAGGG
AGCAGTGATCTTATCAGCTCAGATAGTCATTCGGATTCTTTCAGCGCTTTCCAATATGAT
GGCCGAAAATTTGACAATTTTGGCTTTGGAACCGACACTGGGGTTACGTCCTCTGCTGAT
GTGGATTCAGGTTCTGGCCATCATCAGAGTGCTGAAGAGCAGGAAGTGGCTAGTCTAACT
ACTCTTCATATAGATTCTGAAACAAGCAGTCTTAATCAGCAAGCTTTCTCTGCTGAAGTT
GCAACTATTACTGGTTCAGAAAGTGCTTCCCCAGTCCACTCACCTCTGGGCTCCAGGTCA
CAGACTCCTTCCCCTTCTACATTGAATATAGATCACATGGAACAGAAGGATCTGCAGCTC
GACGAGAAGCTCCACCACTCTGTTCTTCAGACACCAGATGATCTAGAAATTAGTGAATTT
CCATCAGAATGTTGTAGTGTGATGGCAGGAGGTACTCTGACTGGATGGCATGCTGATGTT
GCTACTGTAATGTGGCGAAGAATGCTAGGCATTTTGGGAGATGTAAATTCAATCATGGAT
CCTGAAATACATGCTCAAGTTTTTGATTACCTCTGTGAACTTTGGCAGAATCTAGCTAAG
ATTAGAGATAACCTTGGCATTTCAACTGATAACCTGACCTCCCCTTCTCCACCAGTTTTA
ATTCCTCCACTGAGAATTCTTACACCTTGGCTTTTTAAGGCAACCATGTTGACTGATAAA
TATAAACAAGGTAAATTACATGCATATAAACTTATTTGTAATACAATGAAAAGAAGACAA
GATGTTTCTCCAAATAGAGATTTTCTAACACATTTCTACAATATAATGCATTGTGGATTA
CTTCATATTGACCAGGATATTGTCAATACAATCATCAAACACTGCTCACCTCAATTTTTT
TCACTTGGTTTGCCTGGTGCCACAATGCTTATTATGGATTTTATTGTAGCAGCTGGTAGA
GTGGCTTCTTCAGCTTTTCTCAATGCACCAAGAGTAGAAGCACAAGTTCTTCTGGGATCT
TTGGTTTGCTTTCCCAACTTATATTGTGAACTGCCTTCTCTTCATCCCAACATTCCTGAT
GTTGCTGTGTCTCAGTTTACAGATGTTAAGGAACTTATAATCAAAACTGTATTAAGCTCG
GCAAGAGATGAGCCCTCTGGTCCTGCACGATGTGTAGCACTTTGTAGTTTAGGTATTTGG
ATTTGTGAAGAACTAGTCCATGAGTCTCATCATCCTCAAATTAAGGAAGCTCTGAATGTG
ATTTGTGTTTCCTTAAAGTTTACTAATAAAACAGTAGCCCACGTAGCTTGTAACATGCTT
CACATGCTGGTTCATTATGTACCTAGACTTCAGATTTACCAGCCTGATTCTCCCTTGAAA
ATTATTCAAATCCTAATAGCTACCATCACCCATCTTTTACCAAGTACAGAGGCTTCATCT
TATGAAATGGACAAGAGGTTGGTAGTATCTTTACTTCTCTGCCTTCTGGACTGGATCATG
GCCTTACCTCTAAAGACACTGCTCCAACCATTTCATGCTACGGGAGCAGAAAGCGATAAA
ACAGAAAAATCTGTTCTCAATTGCATTTATAAGGTTTTACATGGGTGTGTTTATGGAGCT
CAGTGTTTTAGCAATCCAAGGTATTTTCCCATGAGCCTCTCTGATTTGGCATCTGTAGAT
TATGATCCTTTTATGCATTTGGAAAGTCTGAAAGAGCCTGAGCCTCTGCACTCTCCTGAC
TCAGAACGATCTTCTAAACTCCAGCCAGTAACAGAAGTGAAAACTCAAATGCAGCATGGA
TTAATCTCTATAGCAGCCCGCACTGTTATTACACATCTGGTAAATCACCTGGGCCATTAT
CCAATGAGCGGTGGTCCTGCTATGCTAACAAGTCAGGTGTGTGAAAATCACGACAATCAT
TACAGTGAAAGTACTGAACTTTCTCCTGAACTCTTTGAGAGTCCAAATATCCAGTTCTTT
GTGTTAAATAATACAACCTTAGTGTCCTGTATCCAGATCAGATCAGAAGAGAATATGCCT
GGAGGAGGTTTATCTGCTGGCCTTGCATCAGCCAATTCAAATGTCAGAATCATAGTACGT
GATCTCTCTGGAAAATATTCATGGGATTCTGCTATACTGTATGGCCCACCTCCTGTAAGT
GGCTTGTCAGAACCTACATCTTTCATGCTTTCATTGTCTCACCAAGAGAAGCCAGAAGAG
CCTCCGACATCTAATGAATGCTTAGAAGATATAACCGTAAAAGATGGACTTTCTCTCCAG
TTTAAAAGATTTAGAGAAACTGTACCAACTTGGGATACAATAAGAGATGAAGAAGATGTT
CTTGATGAGCTCTTGCAGTATTTGGGTGTTACTAGTCCTGAATGCTTACAGAGAACTGGA
ATCTCACTTAATATTCCTGCTCCACAACCTGTGTGCATTTCTGAAAAACAAGAAAATGAT
GTTATTAATGCTATCCTTAAGCAACATACAGAAGAAAAAGAATTTGTTGAGAAGCACTTT
AATGACTTAAACATGAAAGCTGTGGAACAAGATGAACCAATACCTCAAAAACCTCAGTCA
GCATTTTATTATTGCAGATTGCTTCTTAGTATATTGGGAATGAATTCCTGGGACAAACGG
AGGAGCTTTCATCTCCTGAAGAAAAATGAAAAGCTACTTAGAGAACTTAGGAACTTGGAT
TCAAGGCAGTGCCGAGAGACACACAAGATTGCAGTATTTTATGTTGCTGAAGGACAAGAA
GACAAACACTCCATTCTCACCAATACAGGAGGAAGTCAAGCATATGAAGATTTTGTAGCT
GGTCTTGGTTGGGAGGTAAATCTTACAAACCATTGTGGTTTTATGGGAGGACTACAAAAA
AACAAAAGCACTGGATTGACCACTCCATATTTTGCTACCTCTACAGTAGAGGTAATATTT
CACGTGTCAACAAGAATGCCTTCTGATTCTGATGATTCTTTGACCAAAAAATTGAGACAT
TTGGGAAATGATGAAGTGCACATTGTTTGGTCAGAGCATACTAGAGACTACAGGAGAGGA
ATTATTCCCACAGAATTTGGTGATGTCCTTATTGTAATATATCCAATGAAAAATCACATG
TTCAGTATTCAGATAATGAAAAAACCAGAGGTTCCCTTCTTTGGTCCCCTTTTTGATGGT
GCTATTGTGAATGGAAAGGTTCTACCCATTATGGTTAGAGCAACAGCTATAAATGCAAGC
CGTGCTCTGAAATCTCTGATTCCATTGTATCAAAACTTCTATGAGGAGAGAGCACGATAC
CTGCAAACAATTGTCCAGCACCACTTAGAACCAACAACATTTGAAGATTTTGCAGCACAG
GTTTTTTCTCCAGCTCCCTACCACCATTTACCATCTGATGCCGGCTCCTACCCAGAGATT
CTACCCAGTGAAACTCCCACAGCAACGCAGGTAGATGGGGCTGACCTGGCCTCTCCAATG
TCTCCTCGAACTAGCAAAAGCCGCATGTCCATGAAGCTGCGTCGTTCCTCTGGCTCAGCC
AATAAATCCTAA
Restriction Sites Please inquire     
ACCN NM_194301
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_194301.2, NP_919277.2
RefSeq Size 7851 bp
RefSeq ORF 6252 bp
Locus ID 253959
UniProt ID Q6GYQ0
Gene Summary This gene encodes a major subunit of the RAL-GTPase activating protein. A similar protein in mouse binds E12, a transcriptional regulator of immunoglobulin genes. The mouse protein also functions in skeletal muscle by binding to the regulatory 14-3-3 proteins upon stimulation with insulin or muscle contraction. A pseudogene of this gene has been identified on chromosome 9. [provided by RefSeq, Oct 2016]
Transcript Variant: This variant (2) differs in the 3' UTR and lacks an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is longer and has a distinct C-terminus, compared to isoform 1. Isoforms 2 and 4 are the same length, but have distinct protein sequences. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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