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ATG9B (NM_173681) Human Untagged Clone
CAT＃: SC306912
ATG9B (untagged)-Human ATG9 autophagy related 9 homolog B (S. cerevisiae) (ATG9B)
CNY 17,860.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | APG9L2; NOS3AS; SONE |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC306912 representing NM_173681.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTGAGCCGAATGGGCTGGGGGGGGAGAAGAAGGCGGCTGGGGCGGTGGGGAGATCTGGGGCCCGGA TCGGTGCCCCTCCTCCCCATGCCACTGCCACCTCCTCCTCCTCCTTCATGCCGGGGACCTGGGGGAGGG AGGATCTCCATCTTCTCTCTGTCCCCTGCCCCTCATACAAGAAGCTCCCCCTCCTCATTTTCCCCTCCC ACCGCAGGGCCCCCTTGCTCAGTGCTACAGGGGACAGGGGCTTCTCAGTCTTGCCACAGTGCTCTCCCT ATCCCAGCCACCCCCCCAACACAGGCTCAACCTGCAATGACACCTGCCTCTGCATCTCCCTCCTGGGGA TCCCACTCCACCCCACCCCTGGCCCCGGCAACCCCCACTCCCTCACAGCAGTGCCCCCAGGACTCTCCT GGGCTGCGGGTAGGCCCTTTGATCCCTGAACAGGATTATGAGCGGCTGGAGGACTGTGACCCTGAGGGG TCCCAAGACTCACCCATCCACGGGGAGGAGCAGCAACCCCTGCTTCATGTCCCTGAAGGGCTCCGAGGC TCCTGGCATCACATCCAGAACCTGGACAGTTTCTTCACCAAGATCTACAGCTACCACCAGCGGAATGGC TTTGCCTGCATCTTGCTGGAGGATGTCTTCCAGCTGGGACAATTTATTTTCATTGTCACCTTCACAACC TTCCTCCTTCGATGCGTGGATTACAATGTTCTCTTTGCCAACCAACCAAGTAACCATACCAGACCTGGG CCGTTCCACAGCAAAGTGACCCTGTCAGATGCCATCCTACCCTCAGCCCAGTGTGCTGAGAGGATCCGC TCCAGCCCGCTGCTGGTCCTCCTCCTGGTCCTGGCTGCCGGCTTCTGGCTGGTCCAACTGCTTCGCTCA GTCTGCAACCTCTTCAGCTACTGGGACATCCAGGTGTTTTACAGGGAGGCCCTGCACATCCCCCCGGAG GAGCTGAGCTCGGTTCCCTGGGCAGAGGTGCAGTCCCGCCTCTTGGCACTGCAGCGGAGCGGGGGCCTG TGCGTGCAGCCGCGGCCCCTGACGGAGCTGGACATCCACCACCGCATCCTGCGCTACACCAACTACCAG GTGGCGCTGGCCAACAAAGGCCTGCTGCCGGCCCGCTGCCCGCTGCCCTGGGGAGGCAGTGCGGCTTTC CTCAGCCGCGGCCTGGCGCTCAATGTCGACCTGCTGCTCTTCCGCGGTCCCTTCTCGCTCTTCCGCGGG GGCTGGGAGCTGCCGCACGCCTACAAGCGCAGCGACCAGCGGGGCGCCCTAGCAGCGCGCTGGGGGCGC ACAGTGCTGCTGCTGGCCGCCCTGAACCTGGCGCTGAGCCCGCTGGTGCTGGCCTGGCAGGTTCTGCAC GTCTTCTATAGCCACGTGGAGCTGCTGCGGCGCGAGCCTGGCGCGCTGGGGGCGCGCGGCTGGTCCCGC CTGGCGCGCTTGCAGCTGCGCCACTTCAACGAGCTGCCGCACGAGCTGCGCGCGCGCCTGGCCCGCGCC TACCGCCCCGCCGCCGCCTTCCTGCGCACCGCTGCGCCCCCCGCGCCCCTGCGCACGCTGCTGGCCCGC CAGCTCGTTTTCTTCGCGGGTGCACTCTTCGCCGCGCTGCTTGTGCTCACCGTCTACGACGAGGACGTG CTAGCCGTGGAGCACGTGCTCACCGCCATGACCGCGCTCGGGGTCACCGCCACCGTCGCCAGGTCTTTC ATTCCGGAAGAGCAGTGCCAGGGTCGTGCGCCGCAGCTCCTGCTGCAGACAGCCCTGGCCCACATGCAC TACCTCCCGGAGGAGCCCGGCCCCGGCGGCAGGGACCGCGCCTACCGGCAGATGGCGCAGCTGCTGCAG TACCGAGCGGTCTCCCTCCTGGAGGAGCTCCTGTCCCCGCTCCTCACCCCGCTGTTTCTGCTTTTCTGG TTCCGCCCTCGTGCCCTGGAGATTATCGACTTTTTTCATCACTTCACTGTGGATGTGGCTGGGGTTGGG GACATCTGTTCCTTTGCCCTTATGGATGTGAAGCGCCACGGACACCCTCAGTGGCTCTCGGCGGGACAG ACTGAGGCCTCGCTGTCTCAGCGTGCGGAGGACGGCAAGACTGAGCTTTCTTTGATGCGGTTCTCCCTG GCGCATCCACTCTGGCGCCCCCCAGGGCACAGCTCTAAGTTTCTTGGGCACCTCTGGGGCCGAGTACAA CAAGATGCAGCTGCCTGGGGTGCCACCTCGGCTCGCGGCCCCTCCACCCCGGGGGTGCTCAGCAACTGC ACCTCGCCCCTGCCTGAGGCCTTCCTGGCCAACCTCTTCGTGCACCCTCTCCTGCCTCCGAGAGATCTG AGCCCGACAGCCCCCTGTCCAGCTGCGGCCACAGCCAGCCTCCTTGCCTCCATTTCCCGAATTGCCCAG GACCCCAGCTCTGTGTCCCCAGGAGGCACTGGGGGCCAGAAGCTGGCCCAGCTCCCAGAACTTGCTTCT GCCGAGATGAGTCTCCATGTCATCTACCTGCACCAGCTTCACCAGCAGCAGCAGCAGCAGGAGCCGTGG GGTGAGGCTGCAGCCTCCATCCTGTCCAGGCCCTGCTCCAGCCCCTCACAGCCACCCTCGCCTGATGAG GAGAAGCCATCCTGGTCAAGTGACGGCTCCAGTCCTGCCTCTAGCCCCAGACAACAGTGGGGAACCCAG AAGGCCCGGAATCTGTTCCCCGGAGGGTTTCAGGTGACCACAGACACCCAGAAGGAGCCTGACCGGGCC TCTTGCACTGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_173681 |
| Insert Size | 2775 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_173681.5 |
| RefSeq Size | 4577 bp |
| RefSeq ORF | 2775 bp |
| Locus ID | 285973 |
| Protein Families | Transmembrane |
| MW | 101 kDa |
| Gene Summary | This gene functions in the regulation of autophagy, a lysosomal degradation pathway. This gene also functions as an antisense transcript in the posttranscriptional regulation of the endothelial nitric oxide synthase 3 gene, which has 3' overlap with this gene on the opposite strand. Mutations in this gene and disruption of the autophagy process have been associated with multiple cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012] Transcript Variant: This variant (1) represents the longer transcript and encodes the supported protein. This transcript contains splicing in the 3' UTR that makes it a candidate for nonsense-mediated mRNA decay (NMD). The protein is thought to be valid, but it may be expressed from alternate transcript variants. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224817 | ATG9B (Myc-DDK-tagged)-Human ATG9 autophagy related 9 homolog B (S. cerevisiae) (ATG9B) |
CNY 10,152.00 |
|
| RC224817L1 | Lenti ORF clone of Human ATG9 autophagy related 9 homolog B (S. cerevisiae) (ATG9B), Myc-DDK-tagged |
CNY 12,552.00 |
|
| RC224817L2 | Lenti ORF clone of Human ATG9 autophagy related 9 homolog B (S. cerevisiae) (ATG9B), mGFP tagged |
CNY 9,030.00 |
|
| RC224817L3 | Lenti ORF clone of Human ATG9 autophagy related 9 homolog B (S. cerevisiae) (ATG9B), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC224817L4 | Lenti ORF clone of Human ATG9 autophagy related 9 homolog B (S. cerevisiae) (ATG9B), mGFP tagged |
CNY 12,552.00 |
|
| RG224817 | ATG9B (tGFP-tagged) - Human ATG9 autophagy related 9 homolog B (S. cerevisiae) (ATG9B) |
CNY 7,890.00 |
