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UNC45B (NM_173167) Human Untagged Clone
CAT＃: SC306792
UNC45B (untagged)-Human unc-45 homolog B (C. elegans) (UNC45B), transcript variant 1
CNY 6,824.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CMYA4; CTRCT43; MFM11; SMUNC45; UNC-45B; UNC45 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_173167 edited
ATGGCAGAGGTGGAAGCGGTACAGCTGAAGGAGGAAGGAAACCGGCATTTCCAGCTCCAG GACTACAAGGCCGCCACAAATAGCTACAGCCAGGCCCTGAAGCTGACCAAGGACAAGGCC CTGCTGGCCACGCTTTATCGGAACCGGGCAGCCTGTGGCCTGAAAACGGAGAGCTACATC CAGGCAGCTTCAGATGCCTCCAGAGCCATCGACATCAACTCCTCGGACATCAAGGCTCTG TATCGGCGATGCCAGGCACTGGAGCACCTGGGGAAGCTGGACCAGGCCTTCAAAGACGTG CAGCGTTGTGCCACCCTCGAGCCACGGAACCAGAACTTCCAGGAGATGCTGAGGAGACTC AACACCAGCATTCAGGAGAAGCTCCGAGTGCAGTTCTCCACAGACTCGAGGGTACAGAAG ATGTTTGAGATCCTCTTGGATGAAAACAGTGAGGCTGATAAGCGGGAAAAGGCTGCCAAC AATCTCATTGTCCTAGGCCGTGAGGAAGCAGGGGCTGAGAAGATCTTCCAGAACAATGGA GTAGCCTTGCTACTGCAGCTTCTGGACACTAAGAAGCCTGAGCTGGTGCTGGCTGCAGTG CGGACCCTGTCGGGCATGTGCAGCGGCCACCAAGCCAGAGCCACAGTGATTCTGCATGCA GTGCGGATAGACCGAATCTGTAGCCTCATGGCCGTGGAGAATGAGGAGATGTCTCTGGCT GTCTGCAACCTGCTCCAAGCCATCATTGACTCCTTGTCTGGGGAGGACAAGCGGGAGCAT CGAGGGAAGGAGGAGGCCCTGGTTCTAGACACCAAGAAGGACCTGAAGCAGATCACCAGC CACCTGCTGGACATGCTAGTCAGCAAGAAGGTGTCTGGCCAGGGCAGGGATCAGGCGCTG AACCTGCTCAATAAGAATGTTCCCAGGAAGGACCTTGCCATTCATGACAACTCACGTACC ATCTATGTGGTGGATAATGGTCTGAGGAAGATCCTGAAGGTTGTGGGGCAGGTTCCAGAT CTGCCATCCTGCCTGCCCCTGACTGACAACACCCGCATGCTGGCCTCTATCCTCATCAAC AAGCTCTATGATGACCTGCGCTGTGACCCGGAGCGCGATCACTTCCGCAAGATCTGTGAG GAATATATCACGGGCAAGTTTGACCCCCAGGACATGGACAAGAACTTGAATGCCATCCAG ACAGTGTCAGGGATCCTGCAGGGCCCCTTTGACCTGGGCAACCAGCTGCTGGGACTGAAA GGTGTGATGGAGATGATGGTGGCACTATGTGGCTCAGAGCGCGAGACGGACCAGCTGGTG GCCGTGGAGGCCCTCATCCATGCCTCCACGAAGCTCAGCCGCGCCACCTTCATCATCACC AATGGAGTGTCACTGCTCAAACAGATCTACAAGACCACCAAAAATGAGAAGATCAAGATC CGCACACTGGTGGGACTCTGTAAGCTCGGCTCTGCAGGTGGCACAGACTACGGTCTCAGG CAGTTTGCGGAAGGGTCGACAGAAAAACTGGCCAAACAGTGTCGCAAGTGGCTGTGCAAT ATGTCCATAGACACTCGGACCCGACGCTGGGCAGTGGAGGGCCTGGCCTACCTCACGCTG GACGCTGATGTGAAGGACGACTTTGTCCAGGACGTCCCTGCCCTGCAGGCCATGTTTGAG CTGGCCAAG::::::ACCAGTGACAAGACCATCCTGTACTCGGTGGCCACCACCCTGGTG AACTGCACCAACAGCTACGATGTCAAGGAGGTCATCCCAGAGCTTGTCCAGCTCGCCAAG TTCTCCAAGCAGCATGTGCCCGAGGAACACCCCAAGGACAAGAAGGACTTTATAGACATG CGGGTGAAGCGGCTTCTGAAGGCGGGTGTCATCTCTGCCCTGGCTTGCATGGTGAAAGCA GATAGTGCCATCCTCACTGACCAGACCAAGGAGCTGCTGGCCAGGGTATTCCTGGCACTG TGTGACAACCCAAAGGACCGAGGCACCATTGTGGCTCAAGGTGGTGGCAAGGCCCTGATT CCCCTGGCTTTGGAGGGCACAGATGTGGGCAAGGTGAAGGCAGCCCACGCTCTAGCAAAG ATCGCTGCTGTCTCCAATCCGGACATTGCTTTTCCTGGGGAGCGGGTGTATGAGGTGGTG CGGCCCCTTGTAAGACTCTTGGACACACAGAGGGATGGGCTTCAGAACTATGAGGCTCTC CTAGGCCTCACCAACCTGTCTGGGCGGAGTGACAAACTCCGGCAGAAGATCTTTAAGGAG AGGGCCTTGCCAGACATCGAGAACTACATGTTTGAGAATCATGATCAGCTGCGGCAGGCG GCCACCGAGTGCATGTGCAACATGGTGCTCCACAAGGAGGTACAGGAAAGGTTCTTGGCT GACGGGAATGACCGGCTGAAGCTGGTGGTGCTGCTCTGCGGGGAGGATGATGATAAGGTG CAGAATGCGGCTGCAGGGGCTCTGGCCATGCTGACAGCAGCACACAAGAAACTGTGCCTC AAGATGACTCAAGTGACAACCCAGTGGTTGGAGATCCTCCAGCGGCTTTGCCTGCACGAC CAGCTGTCTGTCCAACACCGGGGCCTGGTCATTGCCTACAACCTACTGGCAGCCGATGCT GAGCTGGCCAAGAAGCTGGTGGAGAGTGAGCTGCTGGAGATCCTGACTGTGGTGGGCAAA CAGGAGCCAGATGAGAAGAAGGCAGAAGTGGTTCAGACAGCCCGAGAATGTCTCATCAAG TGCATGGATTATGGTTTCATTAAACCAGTGTCTTAG |
| Restriction Sites | Please inquire |
| ACCN | NM_173167 |
| Insert Size | 2800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to differ from the protein associated to this reference by three amino acids. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_173167.2, NP_775259.1 |
| RefSeq Size | 5694 bp |
| RefSeq ORF | 2796 bp |
| Locus ID | 146862 |
| UniProt ID | Q8IWX7 |
| Gene Summary | This gene encodes a co-chaperone required for folding and accumulation of type II myosins. The protein consists of three tetratricopeptide repeat motifs at the N-terminus that form a complex with heat shock protein 90, a central region of unknown function that is conserved in all Unc-45 proteins, and a C-terminal Unc-45/Cro1/She4 domain. The protein is expressed at high levels in striated muscle, where its muscle myosin chaperone activity is dependent on heat shock protein 90 acting as a co-chaperone. A missense mutation in this gene has been associated with cataract development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The myosin chaperone UNC45B is involved in lens development and autosomal dominant juvenile cataract
,Hansen, L;Comyn, S;Mang, Y;Lind-Thomsen, A;Myhre, L;Jean, F;Eiberg, H;Tommerup, N;Rosenberg, T;Pilgrim, D;,
Eur. J. Hum. Genet., Feb 2014.
,PubMed ID 24549050
[UNC45B]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223751 | UNC45B (Myc-DDK-tagged)-Human unc-45 homolog B (C. elegans) (UNC45B), transcript variant 1 |
CNY 2,400.00 |
|
| RC223751L3 | Lenti ORF clone of Human unc-45 homolog B (C. elegans) (UNC45B), transcript variant 1, Myc-DDK-tagged |
CNY 11,690.00 |
|
| RC223751L4 | Lenti ORF clone of Human unc-45 homolog B (C. elegans) (UNC45B), transcript variant 1, mGFP tagged |
CNY 11,690.00 |
|
| RG223751 | UNC45B (tGFP-tagged) - Human unc-45 homolog B (C. elegans) (UNC45B), transcript variant 1 |
CNY 4,000.00 |
