United States
Germany
Japan
United Kingdom
China
GPR113 (ADGRF3) (NM_153835) Human Untagged Clone
CAT＃: SC306667
GPR113 (untagged)-Human G protein-coupled receptor 113 (GPR113), transcript variant 3
CNY 7,520.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GPR113; PGR23 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC306667 representing NM_153835.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTCTGTTCGGCTGCCCCACTGCTGCTCCTGGCCACAACTCTTCCCCTGCTGGGGTCACCAGTTGCC CAAGCATCCCAACCTGTAAGTGAGACTGGGGTGAGACCCAGGGAAGGTCTGCAGAGGCGACAATGGGGA CCCCTGATTGGGAGAGACAAAGCATGGAATGAAAGGATAGACAGACCCTTCCCTGCCTGCCCCATCCCC CTATCTTCTAGCTTTGGCCGATGGCCCAAGGGCCAGACAATGTGGGCCCAGACCTCCACCCTCACCCTG ACAGAGGAGGAGTTGGGACAGAGTCAGGCTGGAGGGGAATCTGGATCTGGGCAGCTCCTGGACCAAGAG AATGGAGCAGGGGAATCAGCGCTGGTCTCCGTCTATGTACATCTGGACTTTCCAGATAAGACCTGGCCC CCTGAACTCTCCAGGACACTGACTCTCCCTGCTGCCTCAGCTTCCTCTTCCCCAAGGCCTCTTCTCACT GGCCTCAGACTCACAACAGAGTGTAATGTCAACCACAAGGGGAATTTCTATTGTGCTTGCCTCTCTGGC TACCAGTGGAACACCAGCATCTGCCTCCATTACCCTCCTTGTCAAAGCCTCCACAACCACCAGCCTTGT GGCTGCCTTGTCTTCAGCCATCCCGAACCCGGGTACTGCCAGTTGCTGCCACCTGGGTCCCCTGTCACC TGCCTCCCTGCAGTCCCCGGGATCCTCAACCTGAACTCCCAGCTGCAGATGCCTGGTGACACGCTGAGC CTGACTCTCCATCTGAGCCAGGAGGCCACCAACCTGAGCTGGTTCCTGAGGCACCCAGGGAGCCCCAGT CCCATCCTCCTGCAGCCAGGGACACAGGTGTCTGTGACTTCCAGCCACGGCCAGGCTGCCCTCAGCGTC TCCAACATGTCCCATCACTGGGCAGGTGAGTACATGAGCTGCTTCGAGGCCCAGGGCTTCAAGTGGAAC CTGTATGAGGTGGTGAGGGTGCCCTTGAAGGCGACAGATGTGGCTCGACTTCCATACCAGCTGTCCATC TCCTGTGCCACCTCCCCTGGCTTCCAGCTGAGCTGCTGCATCCCCAGCACAAACCTGGCCTACACCGCG GCCTGGAGCCCTGGAGAGGGCAGCAAAGCTTCCTCCTTCAACGAGTCAGGCTCTCAGTGCTTTGTGCTG GCTGTTCAGCGCTGCCCGATGGCTGACACCACGTACGCTTGTGACCTGCAGAGCCTGGGCCTGGCTCCA CTCAGGGTCCCCATCTCCATCACCATCATCCAGGATGGAGACATCACCTGCCCTGAGGACGCCTCGGTG CTCACCTGGAATGTCACCAAGGCTGGCCACGTGGCACAGGCCCCATGTCCTGAGAGCAAGAGGGGCATA GTGAGGAGGCTCTGTGGGGCTGACGGAGTCTGGGGGCCGGTCCACAGCAGCTGCACAGATGCGAGGCTC CTGGCCTTGTTCACTAGAACCAAGCTGCTGCAGGCAGGCCAGGGCAGTCCTGCTGAGGAGGTGCCACAG ATCCTGGCACAGCTGCCAGGGCAGGCGGCAGAGGCAAGTTCACCCTCCGACTTACTGACCCTGCTGAGC ACCATGAAATACGTGGCCAAGGTGGTGGCAGAGGCCAGAATACAGCTTGACCGCAGAGCCCTGAAGAAT CTCCTGATTGCCACAGACAAGGTCCTAGATATGGACACCAGGTCTCTGTGGACCCTGGCCCAAGCCCGG AAGCCCTGGGCAGGCTCGACTCTCCTGCTGGCTGTGGAGACCCTGGCATGCAGCCTGTGCCCACAGGAC CACCCCTTCGCCTTCAGCTTACCCAATGTGCTGCTGCAGAGCCAGCTGTTTGGACCCACGTTTCCTGCT GACTACAGCATCTCCTTCCCTACTCGGCCCCCACTGCAGGCTCAGATTCCCAGGCACTCACTGGCCCCA TTGGTCCGTAATGGAACTGAAATAAGTATTACTAGCCTGGTGCTGCGAAAACTGGACCACCTTCTGCCC TCAAACTATGGACAAGGGCTGGGGGATTCCCTCTATGCCACTCCTGGCCTGGTCCTTGTCATTTCCATC ATGGCAGGTGACCGGGCCTTCAGCCAGGGAGAGGTCATCATGGACTTTGGGAACACAGATGGTTCCCCT CACTGTGTCTTCTGGGATCACAGTCTCTTCCAGGGCAGGGGGGGTTGGTCCAAAGAAGGGTGCCAGGCA CAGGTGGCCAGTGCCAGCCCCACTGCTCAGTGCCTCTGCCAGCACCTCACTGCCTTCTCCGTCCTCATG TCCCCACACACTGTTCCGGAAGAACCCGCTCTGGCGCTGCTGACTCAAGTGGGCTTGGGAGCTTCCATA CTGGCGCTGCTTGTGTGCCTGGGTGTGTACTGGCTGGTGTGGAGAGTCGTGGTGCGGAACAAGATCTCC TATTTCCGCCACGCCGCCCTGCTCAACATGGTGTTCTGCTTGCTGGCCGCAGACACTTGCTTCCTGGGC GCCCCATTCCTCTCTCCAGGGCCCCGAAGCCCGCTCTGCCTTGCTGCCGCCTTCCTCTGTCATTTCCTC TACCTGGCCACCTTTTTCTGGATGCTGGCGCAGGCCCTGGTGTTGGCCCACCAGCTGCTCTTTGTCTTT CACCAGCTGGCAAAGCACCGAGTTCTCCCCCTCATGGTGCTCCTGGGCTACCTGTGCCCACTGGGGTTG GCAGGTGTCACCCTGGGGCTCTACCTACCTCAAGGGCAATACCTGAGGGAGGGGGAATGCTGGTTGGAT GGGAAGGGAGGGGCGTTATACACCTTCGTGGGGCCAGTGCTGGCCATCATAGGCGTGAATGGGCTGGTA CTAGCCATGGCCATGCTGAAGTTGCTGAGACCTTCGCTGTCAGAGGGACCCCCAGCAGAGAAGCGCCAA GCTCTGCTGGGGGTGATCAAAGCCCTGCTCATTCTTACACCCATCTTTGGCCTCACCTGGGGGCTGGGC CTGGCCACTCTGTTAGAGGAAGTCTCCACGGTCCCTCATTACATCTTCACCATTCTCAACACCCTCCAG GGCGTCTTCATCCTATTGTTTGGTTGCCTCATGGACAGGAAGATACAAGAAGCTTTGCGCAAACGCTTC TGCCGCGCCCAAGCCCCCAGCTCCACCATCTCCCTGGTGAGTTGCTGCCTTCAGATCCTCAGCTGTGCA TCCAAGAGCATGTCAGAAGGCATTCCATGGCCCTCCTCAGAGGACATGGGCACAGCCAGAAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_153835 |
| Insert Size | 3240 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153835.1 |
| RefSeq Size | 4133 bp |
| RefSeq ORF | 3240 bp |
| Locus ID | 165082 |
| UniProt ID | Q8IZF5 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 116.3 kDa |
| Gene Summary | Orphan receptor.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) has alternate 5' and 3' sequences and lacks two internal in-frame coding exons, as compared to variant 1. The resulting isoform (3) has shorter and different N- and C-termini and lack an internal segment, as compared to isoform 1. CCDS Note: There is only one full-length mRNA, AY358172.1, that supports this CCDS splice variant although it is different at the 3' end. The 3' end of AY358172.1 would cause it to be an NMD candidate. The 3' end is supported by BC043211.1, AK122995.1 and AY358214.1 although these have extra internal exons that are not in the RefSeq. Mouse ESTs, BB730166.1 and BB728829.1, support the 3' end. Human ESTs, BI559800.1, DC308501.1, and BX118758.1, show support for some splice sites but also include additional exons. The protein encoded by this CCDS contains intact PFAM Latrophilin/CL-1-like GPS (GPS) and the 7 transmembrane receptor (Secretin family) (7tm_2) domains. Given the current evidence at this locus, this is the best variant that can be represented; this could change with future transcript evidence. It seems that a combination of AY358172.1 5' and 3' ends and the inclusion of the extra AY140955.1 exons in the middle could also create a valid splice variant but there is not enough evidence for this at this time. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215276 | GPR113 (Myc-DDK-tagged)-Human G protein-coupled receptor 113 (GPR113), transcript variant 3 |
CNY 8,264.00 |
|
| RC215276L3 | Lenti ORF clone of Human G protein-coupled receptor 113 (GPR113), transcript variant 3, Myc-DDK-tagged |
CNY 12,640.00 |
|
| RC215276L4 | Lenti ORF clone of Human G protein-coupled receptor 113 (GPR113), transcript variant 3, mGFP tagged |
CNY 12,640.00 |
|
| RG215276 | GPR113 (tGFP-tagged) - Human G protein-coupled receptor 113 (GPR113), transcript variant 3 |
CNY 11,780.00 |
