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DGKD (NM_152879) Human Untagged Clone
CAT＃: SC306516
DGKD (untagged)-Human diacylglycerol kinase, delta 130kDa (DGKD), transcript variant 2
CNY 23,470.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DGK-delta; dgkd-2; DGKdelta |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC306516 representing NM_152879.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGCGGCGGCGGGCGCCCCTCCGCCGGGTCCCCCGCAACCGCCTCCGCCGCCGCCGCCCGAGGAG TCGTCCGACAGCGAGCCCGAGGCGGAGCCCGGCTCCCCACAGAAGCTCATCCGCAAGGTGTCCACGTCG GGTCAGATCCGACAGAAGACCATCATCAAAGAGGGGATGCTGACCAAACAGAACAATTCATTCCAGCGA TCAAAAAGGAGATACTTTAAGCTTCGAGGGCGAACGCTTTACTATGCCAAAACGGCAAAGTCAATCATA TTTGATGAGGTGGATCTGACAGATGCCAGCGTAGCTGAATCCAGTACCAAAAACGTCAACAACAGTTTT ACGGTCATAACTCCATGCAGGAAGCTCATCTTGTGTGCTGATAACAGAAAAGAAATGGAAGATTGGATT GCAGCATTAAAGACTGTGCAGAACAGGGAGCACTTTGAGCCCACCCAGTACAGCATGGACCACTTCTCA GGGATGCACAATTGGTACGCCTGTTCCCACGCGAGGCCGACCTACTGCAATGTGTGCCGTGAGGCTCTG TCTGGGGTCACGTCGCACGGGCTGTCCTGCGAGGTGTGCAAATTTAAGGCCCACAAGCGCTGTGCTGTG CGTGCAACCAATAACTGCAAGTGGACCACACTGGCCTCGATCGGGAAGGACATCATTGAAGATGCAGAT GGGATTGCAATGCCCCACCAGTGGTTGGAAGGAAACCTACCTGTGAGCGCCAAGTGCACTGTGTGCGAC AAGACCTGTGGCAGTGTGCTGCGCCTGCAGGACTGGCGCTGCCTCTGGTGCAAGGCCATGGTTCACACA TCGTGTAAAGAATCCTTGCTGACCAAGTGCCCACTTGGCCTGTGCAAAGTGTCAGTCATCCCACCCACG GCTCTCAACAGCATCGACTCCGATGGGTTCTGGAAGGCCAGCTGTCCTCCTTCTTGCACAAGCCCACTG TTGGTCTTCGTCAATTCAAAAAGTGGGGACAACCAGGGTGTGAAGTTCCTCAGAAGATTCAAACAGCTA CTAAACCCCGCCCAGGTCTTCGACCTCATGAACGGAGGCCCACACCTCGGCTTACGGTTATTCCAGAAG TTTGACACATTCCGGATTCTGGTTTGTGGCGGGGATGGAAGTGTTGGCTGGGTCCTCTCCGAAATCGAC AGCCTCAACCTTCATAAACAGTGTCAGCTGGGAGTGCTGCCGCTCGGCACAGGGAACGACTTGGCCCGA GTACTGGGCTGGGGCTCAGCCTGCGATGACGACACCCAGCTCCCCCAGATCTTGGAGAAGTTGGAGAGA GCCAGCACCAAGATGCTGGACAGGTGGAGCGTCATGGCATACGAGGCCAAGCTCCCCCGGCAGGCCTCC TCCTCTACCGTCACCGAAGACTTCAGCGAGGATTCCGAGGTACAGCAGATTCTCTTCTATGAAGACTCG GTTGCAGCCCACCTTTCTAAAATCCTCACCTCGGACCAGCACTCGGTGGTCATCTCCTCGGCCAAAGTG CTCTGTGAGACGGTGAAGGACTTCGTGGCACGGGTGGGGAAGGCCTATGAGAAGACGACCGAGAGCTCG GAGGAGTCAGAGGTCATGGCCAAGAAGTGCTCTGTCCTGAAAGAGAAGCTGGATTCCCTTCTCAAGACC TTGGACGATGAGTCCCAGGCCTCGTCCTCTCTGCCCAACCCGCCCCCCACCATTGCCGAGGAGGCTGAA GATGGAGATGGGTCGGGCAGCATCTGCGGTTCCACCGGAGACCGCTTGGTGGCATCAGCTTGCCCGGCC CGGCCGCAGATATTCCGGCCTCGAGAACAGCTCATGCTGAGAGCCAACAGCCTGAAGAAAGCAATTCGT CAGATCATAGAACACACAGAAAAAGCTGTCGATGAGCAGAATGCCCAGACCCAGGAGCAGGAGGGCTTC GTCCTGGGCCTCTCTGAGTCAGAGGAGAAGATGGACCACAGAGTGTGCCCACCACTGTCCCACAGCGAG AGCTTCGGGGTCCCCAAGGGGAGGAGCCAGCGCAAAGTGTCGAAATCTCCGTGTGAAAAGCTGATCAGC AAAGGGAGTCTGTCCCTAGGCAGTTCTGCTTCCCTTCCGCCCCAGCCGGGAAGCCGGGACGGCCTGCCT GCGCTCAACACCAAGATCCTGTACCCAAATGTCCGGGCTGGAATGTCTGGTTCCTTACCCGGTGGCTCA GTCATCAGTCGCCTGTTAATTAATGCTGATCCCTTCAACTCTGAACCAGAAACCCTAGAGTATTACACG GAGAAATGTGTCATGAACAACTATTTTGGCATTGGCCTGGATGCGAAGATATCCCTGGACTTTAACAAC AAGCGCGATGAGCACCCAGAGAAGTGCAGGAGCCGAACCAAGAACATGATGTGGTATGGAGTTCTTGGA ACCAAAGAGTTGCTGCACAGAACCTACAAGAACCTGGAGCAAAAGGTCTTGCTGGAGTGTGACGGGCGA CCCATCCCACTCCCCAGTCTTCAGGGAATTGCTGTCCTTAACATTCCCAGCTATGCCGGAGGAACCAAC TTCTGGGGGGGTACCAAGGAAGATGATACTTTCGCAGCTCCATCATTCGATGACAAGATTCTGGAGGTG GTCGCCGTGTTCGGCAGCATGCAGATGGCCGTCTCTCGAGTCATCAGGCTACAGCATCATCGGATCGCC CAGTGTCGCACGGTGAAGATCTCCATCCTTGGGGATGAGGGCGTGCCTGTGCAGGTGGACGGAGAGGCC TGGGTCCAGCCGCCAGGGTACATTCGGATTGTCCACAAGAACCGGGCACAGACACTGACCAGAGACAGG GCATTTGAGAGCACCCTGAAGTCCTGGGAAGACAAGCAGAAGTGCGAGCTGCCCCGCCCTCCATCCTGT TCCCTGCACCCGGAGATGCTGTCCGAGGAGGAGGCCACCCAGATGGACCAGTTTGGGCAGGCAGCAGGG GTCCTCATTCACAGTATCCGAGAAATAGCTCAGTCTCACCGGGACATGGAGCAGGAACTGGCCCACGCC GTCAATGCCAGCTCCAAGTCCATGGACCGTGTGTATGGCAAGCCCAGAACCACAGAGGGGCTCAACTGC AGCTTCGTCCTGGAAATGGTGAATAACTTCAGAGCTCTGCGCAGTGAGACGGAGCTGCTGCTGTCTGGG AAGATGGCCCTGCAGCTGGATCCGCCTCAGAAGGAGCAGCTGGGGAGTGCTCTTGCCGAGATGGACCGA CAGCTCAGGAGGCTGGCAGACACCCCGTGGCTCTGCCAGTCCGCAGAGCCCGGCGACGAAGAGAGTGTG ATGCTGGATCTTGCCAAGCGCAGTCGCAGTGGTAAATTCCGCCTCGTGACCAAGTTTAAAAAGGAGAAA AACAACAAGAACAAAGAAGCTCACAGTAGCCTGGGAGCCCCGGTTCACCTCTGGGGGACAGAGGAGGTT GCTGCCTGGCTGGAGCACCTCAGTCTCTGTGAGTATAAGGACATCTTCACACGGCACGACATCCGGGGC TCTGAGCTCCTGCACCTGGAGCGGAGGGACCTCAAGGACCTGGGCGTGACCAAGGTGGGCCACATGAAG AGGATCCTGTGTGGCATCAAGGAGCTGAGCCGCAGCGCCCCCGCCGTCGAGGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_152879 |
| Insert Size | 3645 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_152879.2 |
| RefSeq Size | 6294 bp |
| RefSeq ORF | 3645 bp |
| Locus ID | 8527 |
| UniProt ID | Q16760 |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| MW | 134.5 kDa |
| Gene Summary | This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217053 | DGKD (Myc-DDK-tagged)-Human diacylglycerol kinase, delta 130kDa (DGKD), transcript variant 2 |
CNY 8,456.00 |
|
| RC217053L3 | Lenti ORF clone of Human diacylglycerol kinase, delta 130kDa (DGKD), transcript variant 2, Myc-DDK-tagged |
CNY 13,970.00 |
|
| RC217053L4 | Lenti ORF clone of Human diacylglycerol kinase, delta 130kDa (DGKD), transcript variant 2, mGFP tagged |
CNY 13,970.00 |
|
| RG217053 | DGKD (tGFP-tagged) - Human diacylglycerol kinase, delta 130kDa (DGKD), transcript variant 2 |
CNY 13,300.00 |
