ACOT11 (NM_147161) Human Untagged Clone

CAT#: SC306297

ACOT11 (untagged)-Human acyl-CoA thioesterase 11 (ACOT11), transcript variant 2



  "NM_147161" in other vectors (6)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
Anti-ACOT11 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms BFIT; STARD14; THEA; THEM1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC306297 representing NM_147161.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGATCCAGAATGTCGGAAATCACCTGCGACGGGGCTTGGCCTCTGTGTTCTCCAACCGCACATCCCGG
AAGTCAGCCTTACGTGCGGGGAACGACAGTGCCATGGCAGACGGCGAGGGATACCGGAACCCCACGGAG
GTGCAGATGAGCCAGCTGGTGCTGCCCTGCCACACCAACCAACGTGGTGAGCTGAGCGTCGGGCAGCTG
CTCAAGTGGATTGACACCACGGCTTGCCTGTCCGCGGAGAGGCACGCTGGCTGCCCCTGTGTCACAGCT
TCCATGGATGACATCTATTTTGAGCACACCATTAGTGTTGGACAAGTGGTGAATATCAAGGCCAAGGTG
AACCGGGCCTTCAACTCCAGCATGGAGGTGGGCATCCAGGTGGCCTCGGAGGACCTGTGCTCTGAGAAG
CAGTGGAATGTGTGCAAGGCCTTGGCCACCTTCGTGGCCCGCCGAGAGATCACCAAGGTGAAGCTGAAG
CAGATCACGCCGCGGACAGAAGAGGAGAAGATGGAGCACAGTGTGGCGGCTGAGCGCCGGCGCATGCGC
CTTGTCTATGCAGACACCATCAAGGACCTCCTGGCCAACTGCGCCATTCAGGGCGATCTGGAGAGCAGA
GACTGTAGCCGCATGGTGCCGGCTGAGAAGACCCGTGTGGAGAGTGTGGAGCTGGTCCTGCCTCCCCAC
GCCAATCACCAGGGCAACACCTTTGGGGGCCAGATCATGGCCTGGATGGAGAATGTGGCCACCATTGCA
GCCAGCCGGCTCTGCCGTGCCCACCCTACGCTGAAGGCCATTGAAATGTTCCACTTCCGAGGCCCGTCC
CAGGTCGGCGACCGTCTGGTGCTCAAAGCCATCGTGAACAATGCCTTCAAACATAGCATGGAGGTGGGC
GTGTGCGTGGAGGCCTATCGCCAGGAGGCTGAGACCCACCGGCGCCACATCAACAGTGCCTTTATGACC
TTTGTGGTCCTGGACGCAGATGACCAGCCCCAGTTGCTGCCCTGGATTCGGCCCCAGCCCGGCGATGGT
GAGCGGCGGTACCGAGAGGCCAGTGCCAGAAAGAAGATCCGCCTGGACAGGAAGTACATCGTGTCCTGT
AAGCAGACAGAGGTGCCCCTCTCCGTCCCCTGGGACCCTAGCAACCAGGTGTACCTGAGCTACAATAAC
GTCTCCTCCTTGAAGATGCTTGTGGCCAAGGACAACTGGGTGCTGTCCTCGGAGATCAGTCAGGTCCGC
CTGTACACTCTGGAGGATGACAAGTTCCTCTCCTTCCACATGGAGATGGTGGTGCATGTGGATGCAGCC
CAGGCCTTCCTGCTGCTCTCGGACCTGCGTCAGAGGCCAGAGTGGGACAAGCACTACCGGAGCGTGGAG
CTAGTGCAGCAGGTAGACGAGGACGACGCCATCTACCACGTCACCAGCCCTGCCCTCGGAGGTCACACA
AAGCCCCAGGACTTCGTGATCCTGGCCTCGAGGCGGAAGCCTTGTGACAATGGGGACCCCTATGTCATC
GCGCTGAGGTCGGTCACGCTGCCCACACACCGAGAGACGCCAGAGTACAGACGCGGAGAGACCCTCTGC
TCAGGCTTCTGCCTCTGGCGCGAGGGGGACCAGCTGACCAAGGTATCCTACTACAACCAGGCCACCCCA
GGTGTTCTCAACTATGTGACCACCAACGTGGCCGGCCTCTCCTCTGAGTTCTACACCACCTTCAAGGCT
TGTGAGCAGTTTCTCTTGGACAACCGGAATGATCTGGCCCCCAGCCTCCAGACCCTCTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_147161
Insert Size 1785 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_147161.3
RefSeq Size 3181 bp
RefSeq ORF 1785 bp
Locus ID 26027
UniProt ID Q8WXI4
MW 67.2 kDa
Gene Summary This gene encodes a member of the acyl-CoA thioesterase family which catalyse the conversion of activated fatty acids to the corresponding non-esterified fatty acid and coenzyme A. Expression of a mouse homolog in brown adipose tissue is induced by low temperatures and repressed by warm temperatures. Higher levels of expression of the mouse homolog has been found in obesity-resistant mice compared with obesity-prone mice, suggesting a role of acyl-CoA thioesterase 11 in obesity. Alternative splicing results in transcript variants. [provided by RefSeq, Nov 2010]
Transcript Variant: This variant (2) uses alternate exons in the 3' coding region and UTR, compared to variant 1. The encoded isoform (BFIT2) has a distinct C-terminus compared to isoform BFIT1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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