CCDC114 (NM_144577) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CILD20
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC306158 representing NM_144577. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAAGGGGAGAGGCGGGCCTACAGCAAGGAAGTCCACCAGCGCATCAACAAGCAACTTGAGGAGATC CGGCGCTTGGAGGAGGTACGGGGCGATCTCCAGGTGCAGATCAGCGCAGCCCAGAACCAGGTCAAGCGG CTTCGGGACAGTCAGCGGCTGGAGAACATGGACCGCCTGCTGAAGGGCCGGGCCCAGGTGCAGGCGGAG ATCGAGGAGCTGCAGGAGCAGACCAGGGCCCTGGACAAGCAGATCCAGGAGTGGGAGACGCGGATCTTT ACCCACAGTAAGAATGTCAGGTCCCCGGGATTCATCCTGGATCAGAAGGTCAAGATCAGGCGAAGGATC AGGATCCTAGAAAACCAGTTGGACAGGGTCACCTGTCACTTTGACAACCAGCTGGTACGGAATGCGGCC CTGCGGGAGGAGCTGGATCTGCTGCGGATCGACAGGAACCGCTATCTGAACGTGGACCGCAAGCTGAAG AAGGAGATCCACCACCTGCATCACCTGGTCAGCACCCTTATCCTCTCCTCCACCTCTGCCTACGCCGTC AGGGAGGAGGCGAAGGCCAAGATGGGCTTGCTGCGGGAGCGCGCGGAGAAAGAGGAGGCCCAGAGCGAG ATGGAGGCGCAGGTCCTGCAGCGGCAGATCTTGCACCTGGAGCAGCTGCACCACTTCCTCAAGCTCAAG AACAACGACCGGCAGCCGGATCCCGATGTCCTGGAGAAGCGTGAAAAGCAGGCCGGGGAGGTGGCCGAG GGCGTCTGGAAGACCTCCCAGGAGAGGCTGGTGCTTTGCTACGAGGACGCCCTGAATAAACTGTCCCAG CTGATGGGGGAGAGTGACCCTGACCTGTTGGTGCAGAAGTATCTGGAGATCGAGGAGCGCAACTTTGCT GAGTTCAACTTCATCAACGAGCAGAACTTGGAGCTGGAGCATGTGCAGGAAGAGATCAAGGAGATGCAG GAGGCTTTGGTGAGCGCACGTGCCAGCAAGGATGACCAGCATTTGCTGCAGGAGCAGCAGCAGAAGGTG TTGCAGCAGCGCATGGACAAGGTGCACTCGGAGGCTGAGCGCCTTGAGGCCCGCTTCCAGGATGTGCGG GGACAGCTGGAGAAGCTCAAGGCTGATATCCAGCTCCTCTTCACCAAGGCCCATTGCGACAGCAGCATG ATCGATGACCTCCTTGGGGTCAAGACCAGCATGGGAGACCGGGACATGGGCCTCTTCCTGAGCCTCATT GAGAAGCGGCTGGTGGAGCTCCTGACAGTGCAGGCCTTCCTACATGCCCAGAGCTTCACCTCCCTGGCC GACGCTGCCCTCCTAGTGCTGGGCCAGAGCCTGGAGGACCTTCCGAAGAAGATGGCCCCACTTCAGCCC CCTGACACTCTAGAAGACCCCCCGGGTTTTGAGGCCAGCGATGACTACCCCATGAGCAGGGAGGAGCTG CTGAGCCAAGTGGAGAAGCTGGTGGAGCTCCAGGAGCAGGCGGAGGCGCAGCGCCAGAAGGACCTGGCC GCCGCCGCCGCGAAGCTGGACGGCACCCTGAGCGTGGACCTGGCCAGCACCCAGAGGGCCGGCTCCAGT ACCGTCCTGGTGCCCACCAGGCACCCCCATGCCATCCCCGGGTCCATTTTGAGCCACAAGACTAGCAGA GACCGTGGCTCTCTTGGCCACGTCACTTTTGGCGGCCTCAGCTCCAGCACTGGGCATTTGCCCAGCCAC ATCACGCACGGTGACCCCAACACTGGCCACGTGACCTTCGGCTCCACCAGTGCCTCGAGTGGGGGCCAC GTGACCTTCAGACCCGTCAGCGCCAGCAGCTACCTGGGCTCCACTGGATACGTGGGGTCCAGCAGGGGC GGAGAAAACACAGAGGGTGGTGTGGAGAGCGGAGGCACAGCGTCTGATTCGAGCGGAGGCCTCGGGTCC AGCAGAGACCACGTCTCCAGCACCGGCCCTGCCTCCAGCACTGGCCCGGGCTCCTCCACCAGCAAAGAC TCCCGGGGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_144577
Insert Size	2013 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_144577.3
RefSeq Size	3234 bp
RefSeq ORF	2013 bp
Locus ID	93233
UniProt ID	Q96M63
Protein Families	Druggable Genome
MW	75 kDa
Gene Summary	This gene encodes a coiled-coil domain-containing protein that is a component of the outer dynein arm docking complex in cilia cells. Mutations in this gene may cause primary ciliary dyskinesia 20. [provided by RefSeq, May 2013]

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