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A1CF (NM_138932) Human Untagged Clone
CAT＃: SC306092
A1CF (untagged)-Human APOBEC1 complementation factor (A1CF), transcript variant 2
CNY 4,432.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ACF; ACF64; ACF65; APOBEC1CF; ASP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_138932 edited
GCTGTGCCAAAAACCTGTGGATTTTCTCTACAAAAATTATTGAGCAACCCTAATTAACCT GATTTTTTGCTGATAATCACTCTCAATGGAATCAAATCACAAATCCGGGGATGGATTGAG CGGCACTCAGAAGGAAGCAGCCCTCCGCGCACTGGTCCAGCGCACAGGATATAGCTTGGT CCAGGAAAATGGACAAAGAAAATATGGTGGCCCTCCACCTGGTTGGGATGCTGCACCCCC TGAAAGGGGCTGTGAAATTTTTATTGGAAAACTTCCCCGAGACCTTTTTGAGGATGAGCT TATACCATTATGTGAAAAAATCGGTAAAATTTATGAAATGAGAATGATGATGGATTTTAA TGGCAACAATAGAGGATATGCATTTGTAACATTTTCAAATAAAGTGGAAGCCAAGAATGC AATCAAGCAACTTAATAATTATGAAATTAGAAATGGGCGCCTCTTAGGGGTTTGTGCCAG TGTGGACAACTGCCGATTATTTGTTGGGGGCATCCCAAAAACCAAAAAGAGAGAAGAAAT CTTATCGGAGATGAAAAAGGTTACTGAAGGTGTTGTCGATGTCATCGTCTACCCAAGCGC TGCAGATAAAACCAAAAACCGAGGCTTTGCCTTCGTGGAGTATGAGAGTCATCGAGCAGC TGCCATGGCGAGGAGGAAACTGCTACCAGGAAGAATTCAGTTATGGGGACATGGTATTGC AGTAGACTGGGCAGAGCCAGAAGTAGAAGTTGATGAAGATACAATGTCTTCAGTGAAAAT CCTATATGTAAGAAATCTTATGCTGTCTACCTCTGAAGAGATGATTGAAAAGGAATTCAA CAATATCAAACCAGGTGCTGTGGAGAGGGTGAAGAAAATTCGAGACTATGCTTTTGTGCA CTTCAGTAACCGAGAAGATGCAGTTGAGGCTATGAAAGCTTTAAATGGCAAGGTGCTGGA TGGTTCCCCCATTGAAGTCACCCTAGCAAAACCAGTGGACAAGGACAGTTATGTTAGGTA TACCCGAGGCACAGGTGGAAGGGGCACCATGCTGCAAGGAGAGTATACCTACTCTTTGGG CCAAGTTTATGATCCCACCACAACCTACCTTGGAGCTCCTGTCTTCTATGCCCCCCAGAC CTATGCAGCAATTCCCAGTCTTCATTTCCCAGCCACCAAAGGACATCTCAGCAACAGAGC CATTATCCGAGCCCCTTCTGTTAGAGAAATTTACATGAATGTACCTGTAGGGGCTGCGGG AGTGAGAGGACTGGGCGGCCGTGGCTATTTGGCATACACAGGCCTGGGTCGAGGATACCA GGTCAAAGGAGACAAAAGAGAAGACAAACTCTATGACATTTTACCTGGGATGGAGCTCAC CCCAATGAATCCTGTCACATTAAAACCCCAAGGAATTAAACTCGCTCCCCAGATATTAGA AGAGATTTGTCAGAAAAATAACTGGGGACAGCCAGTGTACCAGCTGCACTCTGCTATTGG ACAAGACCAAAGACAGCTATTCTTGTACAAAATAACTATTCCTGCTCTAGCCAGCCAGAA TCCTGCAATCCACCCTTTCACACCTCCAAAGCTGAGTGCCTTTGTGGATGAAGCAAAGAC GTATGCAGCCGAATACACCCTGCAGACCCTGGGCATCCCCACTGATGGAGGCGATGGCAC CATGGCTACTGCTGCTGCTGCTGCTACTGCTTTCCCAGGATATGCTGTCCCTAATGCAAC TGCACCCGTGTCTGCAGCCCAGCTCAAGCAAGCGGTAACCCTTGGACAAGACTTAGCAGC ATATACAACCTATGAGGTCTACCCAACTTTTGCAGTGACTGCCCGAGGGGATGGATATGG CACCTTCTGAAGATGCTTTTTTAAATTTAAGAATAAGACACACAAAACTCTATTAAAAAA AAAAAAGAAATAAACCTCTAACTCGGTCCCCAATGATC |
| Restriction Sites | Please inquire |
| ACCN | NM_138932 |
| Insert Size | 2000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to be a perfect match to NM_138932.1. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_138932.1, NP_620310.1 |
| RefSeq Size | 2152 bp |
| RefSeq ORF | 1785 bp |
| Locus ID | 29974 |
| UniProt ID | Q9NQ94 |
| Gene Summary | Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 5. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 4. Variants 2 and 8 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216861 | A1CF (Myc-DDK-tagged)-Human APOBEC1 complementation factor (A1CF), transcript variant 2 |
CNY 4,424.00 |
|
| RC216861L3 | Lenti ORF clone of Human APOBEC1 complementation factor (A1CF), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216861L4 | Lenti ORF clone of Human APOBEC1 complementation factor (A1CF), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG216861 | A1CF (tGFP-tagged) - Human APOBEC1 complementation factor (A1CF), transcript variant 2 |
CNY 6,024.00 |
