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Glucokinase (GCK) (NM_033507) Human Untagged Clone
CAT＃: SC305649
GCK (untagged)-Human glucokinase (hexokinase 4) (GCK), transcript variant 2
CNY 3,656.00
CNY 7,410.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FGQTL3; GK; GLK; HHF3; HK4; HKIV; HXKP; LGLK; MODY2; PNDM1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_033507 edited
ATGGCGATGGATGTCACAAGGAGCCAGGCCCAGACAGCCTTGACTCTGGTAGAGCAGATC CTGGCAGAGTTCCAGCTGCAGGAGGAGGACCTGAAGAAGGTGATGAGACGGATGCAGAAG GAGATGGACCGCGGCCTGAGGCTGGAGACCCATGAAGAGGCCAGTGTGAAGATGCTGCCC ACCTACGTGCGCTCCACCCCAGAAGGCTCAGAAGTCGGGGACTTCCTCTCCCTGGACCTG GGTGGCACTAACTTCAGGGTGATGCTGGTGAAGGTGGGAGAAGGTGAGGAGGGGCAGTGG AGCGTGAAGACCAAACACCAGATGTACTCCATCCCCGAGGACGCCATGACCGGCACTGCT GAGATGCTCTTCGACTACATCTCTGAGTGCATCTCCGACTTCCTGGACAAGCATCAGATG AAACACAAGAAGCTGCCCCTGGGCTTCACCTTCTCCTTTCCTGTGAGGCACGAAGACATC GATAAGGGCATCCTTCTCAACTGGACCAAGGGCTTCAAGGCCTCAGGAGCAGAAGGGAAC AATGTCGTGGGGCTTCTGCGAGACGCTATCAAACGGAGAGGGGACTTTGAAATGGATGTG GTGGCAATGGTGAATGACACGGTGGCCACGATGATCTCCTGCTACTACGAAGACCATCAG TGCGAGGTCGGCATGATCGTGGGCACGGGCTGCAATGCCTGCTACATGGAGGAGATGCAG AATGTGGAGCTGGTGGAGGGGGACGAGGGCCGCATGTGCGTCAATACCGAGTGGGGCGCC TTCGGGGACTCCGGCGAGCTGGACGAGTTCCTGCTGGAGTATGACCGCCTGGTGGACGAG AGCTCTGCAAACCCCGGTCAGCAGCTGTATGAGAAGCTCATAGGTGGCAAGTACATGGGC GAGCTGGTGCGGCTTGTGCTGCTCAGGCTCGTGGACGAAAACCTGCTCTTCCACGGGGAG GCCTCCGAGCAGCTGCGCACACGCGGAGCCTTCGAGACGCGCTTCGTGTCGCAGGTGGAG AGCGACACGGGCGACCGCAAGCAGATCTACAACATCCTGAGCACGCTGGGGCTGCGACCC TCGACCACCGACTGCGACATCGTGCGCCGCGCCTGCGAGAGCGTGTCTACGCGCGCTGCG CACATGTGCTCGGCGGGGCTGGCGGGCGTCATCAACCGCATGCGCGAGAGCCGCAGCGAG GACGTAATGCGCATCACTGTGGGCGTGGATGGCTCCGTGTACAAGCTGCACCCCAGCTTC AAGGAGCGGTTCCATGCCAGCGTGCGCAGGCTGACGCCCAGCTGCGAGATCACCTTCATC GAGTCGGAGGAGGGCAGTGGCCGGGGCGCGGCCCTGGTCTCGGCGGTGGCCTGTAAGAAG GCCTGTATGCTGGGCCAGTGAGAGCAGTGGCCGCAAGCGCAGGGAGGATGCCACAGCCCC ACAGCACCCAGGCTCCATGGGGAAGTGCTCCCCACACGTGCTCGCAGCCTGGCGGGGCAG GAGGCCTGGCCTTGTCAGGACCCAGGCCGCCTGCCATACCGCTGGGGAACAGAGCGGGCC TCTTCCCTCAGTTTTTCGGTGGGACAGCCCCAGGGCCCTAACGGGGGTGCGGCAGGAGCA GGAACAGAGACTCTGGAAGCCCCCCACCTTTCTCGCTGGAATCAATTTCCCAGAAGGGAG TTGCTCACTCAGGACTTTGATGCATTTCCACACTGTCAGAGCTGTTGGCCTCGCCTGGGC CCAGGCTCTGGGAAGGGGTGCCCTCTGGATCCTGCTGTGGCCTCACTTCCCTGGGAACTC ATCCTGTGTGGGGAGGCAGCTCCAACAGCTTGACCAGACCTAGACCTGGGCCAAAAGGGC AGCCAGGGGCTGCTCATCACCCAGTCCTGGCCATTTTCTTGCCTGAGGCTCAAGAGGCCC AGGGAGCAATGGGAGGGGGCTCCATGGAGGAGGTGTCCCAAGCTTTGAATACCCCCAGAG ACCTTTTCTCTCCCATACCATCACTGAGTGGCTTGTGATTCTGGGATGGACCCTCGCAGC AGGTGCAAGAGACAGAGCCCCCAAGCCTCTGCCCCAAGGGGCCCACAAAGGGGAGAAGGG CCAGCCCTACATCTTCAGCTCCCATAGCGCTGGCTCAGGAAGAAACCCCAAGCAGCATTC AGCACACCCCAAGGGACAACCCCATCATATGACATGCCACCCTCTCCATGCCCAACCTAA GATTGTGTGGGTTTTTTAATTAAAAATGTTAAAAGTTTTAAAAAAAAAAAAAAAAAAAAA AA >OriGene 5' read for NM_033507 unedited
GAGCAAGTGTATACGACTCCTATAGGGCGGCCGCTGAATTCATGGCGATGGATGTCACAA GGAGCCAGGCCCAGACAGCCTTGACTCTGGTAGAGCAGATCCTGGCAGAGTTCCAGCTGC AGGAGGAGGACCTGAAGAAGGTGATGAGACGGATGCAGAAGGAGATGGACCGCGGCCTGA GGCTGGAGACCCATGAAGAGGCCAGTGTGAAGATGCTGCCCACCTACGTGCGCTCCACCC CAGAAGGCTCAGAAGTCGGGGACTTCCTCTCCCTGGACCTGGGTGGCACTAACTTCAGGG TGATGCTGGTGAAGGTGGGAGAAGGTGAGGAGGGGCAGTGGAGCGTGAAGACCAAACACC AGATGTACTCCATCCCCGAGGACGCCATGACCGGCACTGCTGAGATGCTCTTCGACTACA TCTCTGAGTGCATCTCCGACTTCCTGGACAAGCATCAGATGAAACACAAGAAGCTGCCCC TGGGCTTCACCTTCTCCTTTCCTGTGAGGCACGAAGACATCGATAAGGGCATCCTTCTCA ACTGGACCAAGGGCTTCAAGGCCTCAGGAGCAGAAGGGAACAATGTCGTGGGGCTTCTGC GAGACGCTATCAAACGGAGAGGGGACTTTGAAATGGATGTGGTGGCAATGGTGAATGACA CGGTGGCCACGATGATCTCCTGCTACTACGAAGACCATCAGTGCGAGGTCGGCATGATCG TGGGCACGGGCTGCAATGCCTGCTACATGGAGGAGATGCAGAATGTGGAGCTGGTGGAGG GGACGAGGGCCGCATGTGCGTCAATACCGAGTGGGGCGCCTTCGGGACTCCGGCGAGCTG GACGAGTTCCTGCTGAGTATGACCGCCTGGTGGACGAGAGCTCTGCAAACCCCGTCAGCA GCTGTATGAGAGCTCATAGTGCAGTACATGGGCGAGCTGTGCGCTTGTGCTGCTCAGCTC GTGACAAAACCTGCTTCTTCCAGGGGAGGCCTCCGAGACAGCTG >OriGene 3' genomic read for NM_033507 unedited
CGCGATGCACTTCAGGGCCGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTT TTTTTTTTTAAAACTTTTAACATTTTTAATTAAAAAACCCACACAATCTTAGGTTGGGCA TGGAGAGGGTGGCATGTCATATGATGGGGTTGTCCCTTGGGGTGTGCTGAATGCTGCTTG GGGTTTCTTCCTGAGCCAGCGCTATGGGAGCTGAAGATGTAGGGCTGGCCCTTCTCCCCT TTGTGGGCCCCTTGGGGCAGAGGCTTGGGGGCTCTGTCTCTTGCACCTGCTGCGAGGGTC CATCCCAGAATCACAAGCCACTCAGTGATGGTATGGGAGAGAAAAGGTCTCTGGGGGTAT TCAAAGCTTGGGACACCTCCTCCATGGAGCCCCCTCCCATTGCTCCCTGGGCCTCTTGAG CCTCAGGCAAGAAAATGGCCAGGACTGGGTGATGAGCAGCCCCTGGCTGCCCTTTTGGCC CAGGTCTAGGTCTGGTCAAGCTGTTGGAGCTGCCTCCCCACACAGGATGAGTTCCCAGGG AAGTGAGGCCACAGCAGGATCCAGAGGGCACCCCTTCCCAGAGCCTGGGCCCAGGCGAGG CCAACAGCTCTGACAGTGTGGAAATGCATCAAAGTCCTGAGTGAGCAACTCCCTTCTGGG AAATTGATTCCAGCGAGAAAGGTGGGGGGCTTCCAGAGTCTCTGTTCCTGCTCCTGCCGC ACCCCCGTTAGGGCCCTGGGGCTGTCCCACCGAAAACTGAGGAAGAGGCCCGCTCTGTTC CCAGCGTATTTGGCAGGCGCTGGTCTGACAGCAGCCTCTGCCCGCAGCTGCAGCACGTGT GGGGAGCACTTCCATGAGCTGGGTGCTGGGGGCTGTGCATCTCCTGCGCTTGCG |
| Restriction Sites | Please inquire |
| ACCN | NM_033507 |
| Insert Size | 2300 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033507.1, NP_277042.1 |
| RefSeq Size | 2442 bp |
| RefSeq ORF | 1401 bp |
| Locus ID | 2645 |
| UniProt ID | P35557 |
| Protein Families | Druggable Genome |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Maturity onset diabetes of the young, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus |
| Gene Summary | This gene encodes a member of the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. The use of multiple promoters and alternative splicing of this gene result in distinct protein isoforms that exhibit tissue-specific expression in the pancreas and liver. In the pancreas, this enzyme plays a role in glucose-stimulated insulin secretion, while in the liver, this enzyme is important in glucose uptake and conversion to glycogen. Mutations in this gene that alter enzyme activity have been associated with multiple types of diabetes and hyperinsulinemic hypoglycemia. [provided by RefSeq, Aug 2017] Transcript Variant: This variant (2) encodes the major isoform expressed in liver. Its first exon is specific to the liver transcripts, variants 2 and 3, but it lacks a second liver-specific exon found in variant 3. Isoform 2 has a distinct N-terminus; the remainder of the protein is identical to isoforms 1 and 3. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Role of glucokinase in the subcellular localization of glucokinase regulatory protein
,Jin, L;Guo, T;Li, Z;Lei, Z;Li, H;Mao, Y;Wang, X;Zhou, N;Zhang, Y;Hu, R;Zhang, X;Niu, G;Irwin, DM;Tan, H;,
Int J Mol Sci
,PubMed ID 25849650
[GCK]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222793 | GCK (Myc-DDK-tagged)-Human glucokinase (hexokinase 4) (GCK), transcript variant 2 |
CNY 3,656.00 |
|
| RC222793L1 | Lenti ORF clone of Human glucokinase (hexokinase 4) (GCK), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC222793L2 | Lenti ORF clone of Human glucokinase (hexokinase 4) (GCK), transcript variant 2, mGFP tagged |
CNY 5,990.00 |
|
| RC222793L3 | Lenti ORF clone of Human glucokinase (hexokinase 4) (GCK), transcript variant 2, Myc-DDK-tagged |
CNY 5,990.00 |
|
| RC222793L4 | Lenti ORF clone of Human glucokinase (hexokinase 4) (GCK), transcript variant 2, mGFP tagged |
CNY 5,990.00 |
|
| RG222793 | GCK (tGFP-tagged) - Human glucokinase (hexokinase 4) (GCK), transcript variant 2 |
CNY 5,256.00 |
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