Collagen II (COL2A1) (NM_033150) Human Untagged Clone

CAT#: SC305574

COL2A1 (untagged)-Human collagen, type II, alpha 1 (COL2A1), transcript variant 2



  "NM_033150" in other vectors (2)

CNY 27,270.00


货期*
详询

规格
    • 10 ug

Product images

经常一起买 (3)
Rabbit polyclonal Collagen II antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ANFH; AOM; COL11A3; SEDC; STL1
Vector pCMV6 series
Sequence Data
>NCBI ORF sequence for NM_033150, the custom clone sequence may differ by one or more nucleotides
ATGATTCGCCTCGGGGCTCCCCAGACGCTGGTGCTGCTGACGCTGCTCGTCGCCGCTGTC
CTTCGGTGTCAGGGCCAGGATGTCCGGCAACCAGGACCAAAGGGACAGAAAGGAGAACCT
GGAGACATCAAGGATATTGTAGGACCCAAAGGACCTCCTGGGCCTCAGGGACCTGCAGGG
GAACAAGGACCCAGAGGGGATCGTGGTGACAAAGGTGAAAAAGGTGCCCCTGGACCTCGT
GGCAGAGATGGAGAACCTGGGACCCCTGGAAATCCTGGCCCCCCTGGTCCTCCCGGCCCC
CCTGGTCCCCCTGGTCTTGGTGGAAACTTTGCTGCCCAGATGGCTGGAGGATTTGATGAA
AAGGCTGGTGGCGCCCAGTTGGGAGTAATGCAAGGACCAATGGGCCCCATGGGACCTCGA
GGACCTCCAGGCCCTGCAGGTGCTCCTGGGCCTCAAGGATTTCAAGGCAATCCTGGTGAA
CCTGGTGAACCTGGTGTCTCTGGTCCCATGGGTCCCCGTGGTCCTCCTGGTCCCCCTGGA
AAGCCTGGTGATGATGGTGAAGCTGGAAAACCTGGAAAAGCTGGTGAAAGGGGTCCGCCT
GGTCCTCAGGGTGCTCGTGGTTTCCCAGGAACCCCAGGCCTTCCTGGTGTCAAAGGTCAC
AGAGGTTATCCAGGCCTGGACGGTGCTAAGGGAGAGGCGGGTGCTCCTGGTGTGAAGGGT
GAGAGTGGTTCCCCGGGTGAGAACGGATCTCCGGGCCCAATGGGTCCTCGTGGCCTGCCT
GGTGAAAGAGGACGGACTGGCCCTGCTGGCGCTGCGGGTGCCCGAGGCAACGATGGTCAG
CCAGGCCCCGCAGGGCCTCCGGGTCCTGTCGGTCCTGCTGGTGGTCCTGGCTTCCCTGGT
GCTCCTGGAGCCAAGGGTGAAGCCGGCCCCACTGGTGCCCGTGGTCCTGAAGGTGCTCAA
GGTCCTCGCGGTGAACCTGGTACTCCTGGGTCCCCTGGGCCTGCTGGTGCCTCCGGTAAC
CCTGGAACAGATGGAATTCCTGGAGCCAAAGGATCTGCTGGTGCTCCTGGCATTGCTGGT
GCTCCTGGCTTCCCTGGGCCACGGGGCCCTCCTGGCCCTCAAGGTGCAACTGGTCCTCTG
GGCCCGAAAGGTCAGACGGGTGAACCTGGTATTGCTGGCTTCAAAGGTGAACAAGGCCCC
AAGGGAGAACCTGGCCCTGCTGGCCCCCAGGGAGCCCCTGGACCCGCTGGTGAAGAAGGC
AAGAGAGGTGCCCGTGGAGAGCCTGGTGGCGTTGGGCCCATCGGTCCCCCTGGAGAAAGA
GGTGCTCCCGGCAACCGCGGTTTCCCAGGTCAAGATGGTCTGGCAGGTCCCAAGGGAGCC
CCTGGAGAGCGAGGGCCCAGTGGTCTTGCTGGCCCCAAGGGAGCCAACGGTGACCCTGGC
CGTCCTGGAGAACCTGGCCTTCCTGGAGCCCGGGGTCTCACTGGCCGCCCTGGTGATGCT
GGTCCTCAAGGCAAAGTTGGCCCTTCTGGAGCCCCTGGTGAAGATGGTCGTCCTGGACCT
CCAGGTCCTCAGGGGGCTCGTGGGCAGCCTGGTGTCATGGGTTTCCCTGGCCCCAAAGGT
GCCAACGGTGAGCCTGGCAAAGCTGGTGAGAAGGGACTGCCTGGTGCTCCTGGTCTGAGG
GGTCTTCCTGGCAAAGATGGTGAGACAGGTGCTGCAGGACCCCCTGGCCCTGCTGGACCT
GCTGGTGAACGAGGCGAGCAGGGTGCTCCTGGGCCATCTGGGTTCCAGGGACTTCCTGGC
CCTCCTGGTCCCCCAGGTGAAGGTGGAAAACCAGGTGACCAGGGTGTTCCCGGTGAAGCT
GGAGCCCCTGGCCTCGTGGGTCCCAGGGGTGAACGAGGTTTCCCAGGTGAACGTGGCTCT
CCCGGTGCCCAGGGCCTCCAGGGTCCCCGTGGCCTCCCCGGCACTCCTGGCACTGATGGT
CCCAAAGGTGCATCTGGCCCAGCAGGCCCCCCTGGGGCTCAGGGCCCTCCAGGTCTTCAG
GGAATGCCTGGCGAGAGGGGAGCAGCTGGTATCGCTGGGCCCAAAGGCGACAGGGGTGAC
GTTGGTGAGAAAGGCCCTGAGGGAGCCCCTGGAAAGGATGGTGGACGAGGCCTGACAGGT
CCCATTGGCCCCCCTGGCCCAGCTGGTGCTAATGGCGAGAAGGGAGAAGTTGGACCTCCT
GGTCCTGCAGGAAGTGCTGGTGCTCGTGGCGCTCCGGGTGAACGTGGAGAGACTGGGCCC
CCCGGACCAGCGGGATTTGCTGGGCCTCCTGGTGCTGATGGCCAGCCTGGGGCCAAGGGT
GAGCAAGGAGAGGCCGGCCAGAAAGGCGATGCTGGTGCCCCTGGTCCTCAGGGCCCCTCT
GGAGCACCTGGGCCTCAGGGTCCTACTGGAGTGACTGGTCCTAAAGGAGCCCGAGGTGCC
CAAGGCCCCCCGGGAGCCACTGGATTCCCTGGAGCTGCTGGCCGCGTTGGACCCCCAGGC
TCCAATGGCAACCCTGGACCCCCTGGTCCCCCTGGTCCTTCTGGAAAAGATGGTCCCAAA
GGTGCTCGAGGAGACAGCGGCCCCCCTGGCCGAGCTGGTGAACCCGGCCTCCAAGGTCCT
GCTGGACCCCCTGGCGAGAAGGGAGAGCCTGGAGATGACGGTCCCTCTGGTGCCGAAGGT
CCACCAGGTCCCCAGGGTCTGGCTGGTCAGAGAGGCATCGTCGGTCTGCCTGGGCAACGT
GGTGAGAGAGGATTCCCTGGCTTGCCTGGCCCGTCGGGTGAGCCCGGCAAGCAGGGTGCT
CCTGGAGCATCTGGAGACAGAGGTCCTCCTGGCCCCGTGGGTCCTCCTGGCCTGACGGGT
CCTGCAGGTGAACCTGGACGAGAGGGAAGCCCCGGTGCTGATGGCCCCCCTGGCAGAGAT
GGCGCTGCTGGAGTCAAGGGTGATCGTGGTGAGACTGGTGCTGTGGGAGCTCCTGGAGCC
CCTGGGCCCCCTGGCTCCCCTGGCCCCGCTGGTCCAACTGGCAAGCAAGGAGACAGAGGA
GAAGCTGGTGCACAAGGCCCCATGGGACCCTCAGGACCAGCTGGAGCCCGGGGAATCCAG
GGTCCTCAAGGCCCCAGAGGTGACAAAGGAGAGGCTGGAGAGCCTGGCGAGAGAGGCCTG
AAGGGACACCGTGGCTTCACTGGTCTGCAGGGTCTGCCCGGCCCTCCTGGTCCTTCTGGA
GACCAAGGTGCTTCTGGTCCTGCTGGTCCTTCTGGCCCTAGAGGTCCTCCTGGCCCCGTC
GGTCCCTCTGGCAAAGATGGTGCTAATGGAATCCCTGGCCCCATTGGGCCTCCTGGTCCC
CGTGGACGATCAGGCGAAACCGGCCCTGCTGGTCCTCCTGGAAATCCTGGACCCCCTGGT
CCTCCAGGTCCCCCTGGCCCTGGCATCGACATGTCCGCCTTTGCTGGCTTAGGCCCGAGA
GAGAAGGGCCCCGACCCCCTGCAGTACATGCGGGCCGACCAGGCAGCCGGTGGCCTGAGA
CAGCATGACGCCGAGGTGGATGCCACACTCAAGTCCCTCAACAACCAGATTGAGAGCATC
CGCAGCCCCGAGGGCTCCCGCAAGAACCCTGCTCGCACCTGCAGAGACCTGAAACTCTGC
CACCCTGAGTGGAAGAGTGGAGACTACTGGATTGACCCCAACCAAGGCTGCACCTTGGAC
GCCATGAAGGTTTTCTGCAACATGGAGACTGGCGAGACTTGCGTCTACCCCAATCCAGCA
AACGTTCCCAAGAAGAACTGGTGGAGCAGCAAGAGCAAGGAGAAGAAACACATCTGGTTT
GGAGAAACCATCAATGGTGGCTTCCATTTCAGCTATGGAGATGACAATCTGGCTCCCAAC
ACTGCCAACGTCCAGATGACCTTCCTACGCCTGCTGTCCACGGAAGGCTCCCAGAACATC
ACCTACCACTGCAAGAACAGCATTGCCTATCTGGACGAAGCAGCTGGCAACCTCAAGAAG
GCCCTGCTCATCCAGGGCTCCAATGACGTGGAGATCCGGGCAGAGGGCAATAGCAGGTTC
ACGTACACTGCCCTGAAGGATGGCTGCACGAAACATACCGGTAAGTGGGGCAAGACTGTT
ATCGAGTACCGGTCACAGAAGACCTCACGCCTCCCCATCATTGACATTGCACCCATGGAC
ATAGGAGGGCCCGAGCAGGAATTCGGTGTGGACATAGGGCCGGTCTGCTTCTTGTAA
Restriction Sites Please inquire     
ACCN NM_033150
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_033150.1, NP_149162.1
RefSeq Size 4853 bp
RefSeq ORF 4257 bp
Locus ID 1280
UniProt ID P02458
Protein Pathways ECM-receptor interaction, Focal adhesion
Gene Summary This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in a shorter protein. The resulting protein (isoform 2) lacks the von Willebrand factor type C (VWC) domain, compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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