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WNT3A (NM_033131) Human Untagged Clone
CAT＃: SC305570
WNT3A (untagged)-Human wingless-type MMTV integration site family, member 3A (WNT3A)
CNY 5,488.00
| Cited in 4 publications. |
Product images
CNY 1,999.00
CNY 4,070.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_033131 edited
ACGCTCTCGGGGCGGACTCCCGGCCCTCCGCGCCCTCTCGCGCGGCGATGGCCCCACTCG GATACTTCTTACTCCTCTGCAGCCTGAAGCAGGCTCTGGGCAGCTACCCGATCTGGTGGT CGCTGGCTGTTGGGCCACAGTATTCCTCCCTGGGCTCGCAGCCCATCCTGTGTGCCAGCA TCCCGGGCCTGGTCCCCAAGCAGCTCCGCTTCTGCAGGAACTACGTGGAGATCATGCCCA GCGTGGCCGAGGGCATCAAGATTGGCATCCAGGAGTGCCAGCACCAGTTCCGCGGCCGCC GGTGGAACTGCACCACCGTCCACGACAGCCTGGCCATCTTCGGGCCCGTGCTGGACAAAG CTACCAGGGAGTCGGCCTTTGTCCACGCCATTGCCTCAGCCGGTGTGGCCTTTGCAGTGA CACGCTCATGTGCAGAAGGCACGGCCGCCATCTGTGGCTGCAGCAGCCGCCACCAGGGCT CACCAGGCAAGGGCTGGAAGTGGGGTGGCTGTAGCGAGGACATCGAGTTTGGTGGGATGG TGTCTCGGGAGTTCGCCGACGCCCGGGAGAACCGGCCAGATGCCCGCTCAGCCATGAACC GCCACAACAACGAGGCTGGGCGCCAGGCCATCGCCAGCCACATGCACCTCAAGTGCAAGT GCCACGGGCTGTCGGGCAGCTGCGAGGTGAAGACATGCTGGTGGTCGCAACCCGACTTCC GCGCCATCGGTGACTTCCTCAAGGACAAGTACGACAGCGCCTCGGAGATGGTGGTGGAGA AGCACCGGGAGTCCCGCGGCTGGGTGGAGACCCTGCGGCCGCGCTACACCTACTTCAAGG TGCCCACGGAGCGCGACCTGGTCTACTACGAGGCCTCGCCCAACTTCTGCGAGCCCAACC CTGAGACGGGCTCCTTCGGCACGCGCGACCGCACCTGCAACGTCAGCTCGCACGGCATCG ACGGCTGCGACCTGCTGTGCTGCGGCCGCGGCCACAACGCGCGAGCGGAGCGGCGCCGGG AGAAGTGCCGCTGCGTGTTCCACTGGTGCTGCTACGTCAGCTGCCAGGAGTGCACGCGCG TCTACGACGTGCACACCTGCAAGTAGGCACCGGCCGCGGCTCCCCCTGGACGGGGCGGGC CCTGCCTGAGGGTGGGCTTTTCCCTGGGTGGAGCAGGACTCCCACCTAAACGGGGCAGTA CTCCTCCCTGGGGGCGGGACTCCTACCTGGGGAAAGCCTGAAGGAGAAAACA >OriGene 5' read for NM_033131 unedited
GTTCGGCATTNTGTATACGACTCATATAGGGCGGCCGCGATTCAGATCTGGTACCGAGCT CGGATCCACTAGTAACGGCCGCCATGTGCTGGAATTCGCCCTTCACCACGCTCTCGGGGC GGACTCCCGGCCCTCCGCGCCCTCTCGCGCGGCGATGGCCCCACTCGGATACTTCTTACT CCTCTGCAGCCTGAAGCAGGCTCTGGGCAGCTACCCGATCTGGTGGTCGCTGGCTGTTGG GCCACAGTATTCCTCCCTGGGCTCGCAGCCCATCCTGTGTGCCAGCATCCCGGGCCTGGT CCCCAAGCAGCTCCGCTTCTGCAGGAACTACGTGGAGATCATGCCCAGCGTGGCCGAGGG CATCAAGATTGGCATCCAGGAGTGCCAGCACCAGTTCCGCGGCCGCCGGTGGAACTGCAC CACCGTCCACGACAGCCTGGCCATCTTCGGGCCCGTGCTGGACAAAGCTACCAGGGAGTC GGCCTTTGTCCACGCCATTGCCTCAGCCGGTGTGGCCTTTGCAGTGACACGCTCATGTGC AGAAGGCACGGCCGCCATCTGTGGCTGCAGCAGCCGCCACCAGGGCTCACCAGGCAAGGG CTGGAAGTGGGGTGGCTGTAGCGAGGACATCGAGTTTGGTGGGATGGTGTCTCGGGAGTT CGCCGACGCCCGGGAGAAACCGCCAGATGCCCGCTCAGCCATGAACCGNCACAACAACGA GGCTGGGCGCCAGGCCATCGNCAGCCACATGCACCTCAAGTGCAAGTGCCACGGGCTGTC GGGCAGCTGCGAGGTGAAGACATGCTGGGTGGTCGCAACCCCGACTTCCGCGCCATCGC |
| Restriction Sites | Please inquire |
| ACCN | NM_033131 |
| Insert Size | 1300 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033131.2, NP_149122.1 |
| RefSeq Size | 2932 bp |
| RefSeq ORF | 1059 bp |
| Locus ID | 89780 |
| UniProt ID | P56704 |
| Protein Families | Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway |
| Protein Pathways | Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway |
| Gene Summary | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Identification and Characterization of a Small-Molecule Inhibitor of Wnt Signaling in Glioblastoma Cells
,Alessandra De Robertis, Silvia Valensin, Marco Rossi, Patrizia Tunici, Margherita Verani, Antonella De Rosa, Cinzia Giordano, Maurizio Varrone, Arianna Nencini, Carmela Pratelli, Tiziana Benicchi, Annette Bakker, Jeffrey Hill, Kanda Sangthongpitag, Vishal Pendharkar, Boping Liu, Fui Mee Ng, Siew Wen Then, Shi Jing Tai, Seong-Moon Cheong, Xi He, Andrea Caricasole, and Massimiliano Salerno,
Mol. Cancer Ther., Jul 2013; 12: 1180 - 1189.
,PubMed ID 23619303
[Wnt3a]
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Characterization of the Interaction of Sclerostin with the Low Density Lipoprotein Receptor-related Protein (LRP) Family of Wnt Co-receptors
,Gill Holdsworth, Patrick Slocombe, Carl Doyle, Bernadette Sweeney, Vaclav Veverka, Kelly Le Riche, Richard J. Franklin, Joanne Compson, Daniel Brookings, James Turner, Jeffery Kennedy, Rachael Garlish, Jiye Shi, Laura Newnham, David McMillan, Mariusz Muzylak, Mark D. Carr, Alistair J. Henry, Thomas Ceska, and Martyn K. Robinson,
J. Biol. Chem., Aug 2012; 287: 26464 - 26477.
[WNT3A]
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Effects of a glycogen synthase kinase-3β inhibitor (LiCl) on c-myc protein in intervertebral disc cells
,null,
Journal of Cellular Biochemistry
,PubMed ID 21678465
[WNT3A]
|
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Distinct Roles of Glycogen Synthase Kinase (GSK)-3{alpha} and GSK-3ß in Mediating Cardiomyocyte Differentiation in Murine Bone Marrow-derived Mesenchymal Stem Cells
,Jaeyeaon Cho, Pranela Rameshwar, and Junichi Sadoshima,
J. Biol. Chem., Dec 2009; 284: 36647 - 36658
[Wnt3a]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213639 | WNT3A (Myc-DDK-tagged)-Human wingless-type MMTV integration site family, member 3A (WNT3A) |
CNY 3,656.00 |
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| RC213639L1 | Lenti ORF clone of Human wingless-type MMTV integration site family, member 3A (WNT3A), Myc-DDK-tagged |
CNY 5,890.00 |
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| RC213639L2 | Lenti ORF clone of Human wingless-type MMTV integration site family, member 3A (WNT3A), mGFP tagged |
CNY 6,056.00 |
|
| RC213639L3 | Lenti ORF clone of Human wingless-type MMTV integration site family, member 3A (WNT3A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213639L4 | Lenti ORF clone of Human wingless-type MMTV integration site family, member 3A (WNT3A), mGFP tagged |
CNY 6,056.00 |
|
| RG213639 | WNT3A (tGFP-tagged) - Human wingless-type MMTV integration site family, member 3A (WNT3A) |
CNY 5,256.00 |
