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PLCZ1 (NM_033123) Human Untagged Clone
CAT＃: SC305564
PLCZ1 (untagged)-Human phospholipase C, zeta 1 (PLCZ1)
CNY 4,536.00
CNY 9,790.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NYD-SP27; PLC-zeta-1; PLCzeta; SPGF17 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC305564 representing NM_033123.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAAATGAGATGGTTTTTGTCAAAGATTCAGGATGACTTCAGAGGTGGAAAAATTAACCTAGAAAAA ACTCAGAGGTTACTTGAAAAATTAGATATTCGGTGCAGTTATATTCATGTGAAACAGATTTTTAAGGAC AATGACAGGCTGAAACAAGGAAGAATCACCATAGAAGAATTTAGAGCAATTTATCGAATTATCACGCAC AGAGAAGAAATTATTGAGATTTTCAACACATATTCTGAAAACCGGAAAATTCTTTTAGCAAGTAATCTG GCTCAATTTCTGACACAAGAACAATATGCAGCTGAGATGAGTAAAGCTATTGCTTTTGAGATCATTCAG AAATACGAGCCTATCGAAGAAGTTAGGAAAGCACACCAAATGTCATTAGAAGGTTTTACAAGATACATG GATTCACGTGAATGTCTACTGTTTAAAAATGAATGTAGAAAAGTTTATCAAGATATGACTCATCCATTA AATGATTATTTTATTTCATCTTCACATAACACATATTTGGTATCTGATCAATTATTGGGACCAAGTGAC CTTTGGGGATATGTAAGTGCCCTTGTGAAAGGATGCCGTTGTTTGGAGATTGACTGCTGGGATGGAGCA CAAAATGAACCTGTTGTATATCATGGCTACACACTCACAAGCAAACTTCTGTTTAAAACTGTTATCCAA GCTATACACAAGTATGCATTCATGACATCTGACTACCCAGTGGTGCTCTCTTTAGAAAATCACTGCTCC ACTGCCCAACAAGAAGTAATGGCAGACAATTTGCAGGCTACTTTTGGAGAGTCCTTGCTTTCTGATATG CTTGATGATTTTCCTGATACTCTACCATCACCAGAGGCACTAAAATTCAAAATATTAGTTAAAAATAAG AAAATAGGAACCTTAAAGGAAACCCATGAAAGAAAAGGTTCTGATAAGCGTGGAGACAATCAAGACAAG GAAACAGGGGTAAAAAAGTTACCTGGAGTAATGCTTTTCAAGAAAAAGAAGACCAGGAAGCTAAAAATT GCTCTGGCCTTATCTGATCTTGTCATTTATACGAAAGCTGAGAAATTCAAAAGCTTTCAACATTCAAGA TTATATCAGCAATTTAATGAAAATAATTCTATTGGGGAGACACAAGCCCGAAAACTTTCAAAATTGCGA GTCCATGAGTTTATTTTTCACACCAGGAAGTTCATTACCAGAATATATCCCAAAGCAACAAGAGCAGAC TCTTCTAATTTTAATCCCCAAGAATTTTGGAATATAGGTTGTCAAATGGTGGCTTTAAATTTCCAGACC CCTGGTCTGCCCATGGATCTGCAAAATGGGAAATTTTTGGATAATGGTGGTTCTGGATATATTTTGAAA CCACATTTCTTAAGAGAGAGTAAATCATACTTTAACCCAAGTAACATAAAAGAGGGTATGCCAATTACA CTTACAATAAGGCTCATCAGTGGTATCCAGTTGCCTCTTACTCATTCATCATCTAACAAAGGTGATTCA TTAGTAATTATAGAAGTTTTTGGTGTTCCAAATGATCAAATGAAGCAGCAGACTCGTGTAATTAAAAAA AATGCTTTTAGTCCAAGATGGAATGAAACATTCACATTTATTATTCATGTCCCAGAATTGGCATTGATA CGTTTTGTTGTTGAAGGTCAAGGTTTAATAGCAGGAAATGAATTTCTTGGGCAATATACTTTGCCACTT CTATGCATGAACAAAGGTTATCGTCGTATTCCTCTGTTTTCCAGAATGGGTGAGAGCCTTGAGCCTGCT TCACTGTTTGTTTATGTTTGGTACGTCAGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_033123 |
| Insert Size | 1827 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033123.3 |
| RefSeq Size | 2156 bp |
| RefSeq ORF | 1827 bp |
| Locus ID | 89869 |
| UniProt ID | Q86YW0 |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Oocyte meiosis, Phosphatidylinositol signaling system |
| MW | 70.4 kDa |
| Gene Summary | The protein encoded by this gene is a member of the phosphoinositide-specific phospholipase C family. Members in this family, classified into six isotypes that are tissue- and organ-specific, hydrolyze phosphatidylinositol 4,5-bisphosphate just before the phosphate group to yield diacylglycerol and inositol 1,4,5-trisphosphate. This protein localizes to the acrosome in spermatozoa and elicits Ca(2+) oscillations and egg activation during fertilization that leads to early embryonic development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] Transcript Variant: This variant (1) encodes the longest isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212856 | PLCZ1 (Myc-DDK-tagged)-Human phospholipase C, zeta 1 (PLCZ1) |
CNY 6,792.00 |
|
| RC212856L3 | Lenti ORF clone of Human phospholipase C, zeta 1 (PLCZ1), Myc-DDK-tagged |
CNY 7,220.00 |
|
| RC212856L4 | Lenti ORF clone of Human phospholipase C, zeta 1 (PLCZ1), mGFP tagged |
CNY 7,220.00 |
|
| RG212856 | PLCZ1 (tGFP-tagged) - Human phospholipase C, zeta 1 (PLCZ1) |
CNY 8,392.00 |
