PIGZ (NM_025163) Human Untagged Clone
CAT#: SC305240
PIGZ (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class Z (PIGZ)
CNY 7,220.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | GPI-MT-IV; PIG-Z; SMP3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC305240 representing NM_025163.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAGATCTGTGGATCCAGCGTAGCATCTGTAGCAGCTGGGACATCATTCCAGGTTTTGGGCCCGGTG TGTTGGCAACAACTGGATCTGAAGATGGCAGTCAGGGTGCTTTGGGGTGGTCTCAGCCTGCTCCGAGTG CTGTGGTGTCTCCTTCCGCAGACGGGCTATGTGCACCCAGATGAGTTCTTCCAGTCCCCTGAGGTGATG GCAGAGGACATCCTGGGCGTTCAGGCCGCGCGGCCCTGGGAGTTTTACCCCAGCAGCTCCTGCCGCTCG GTGCTCTTCCCCCTGCTGATCTCTGGTTCCACCTTCTGGCTGCTCAGGCTCTGGGAGGAGCTGGGGCCG TGGCCTGGCCTGGTGAGCGGCTATGCGCTGCTGGTGGGGCCTCGACTCCTCCTCACTGCCCTTTCCTTT GCTCTGGACGGGGCCGTGTACCACCTGGCCCCGCCGATGGGGGCGGATCGCTGGAACGCCCTGGCCCTG CTGTCTGGTTCCTACGTCACCCTGGTCTTCTACACAAGGACCTTCTCCAACACCATTGAGGGACTCCTC TTCACGTGGCTGCTGGTGCTGGTATCCTCCCATGTAACGTGGGGCCCTACACGCAAGGAGCCGGCGCCG GGTCCACGGTGGCGCAGCTGGCTTCTTGGAGGCATTGTGGCTGCTGGCTTCTTCAACCGGCCCACCTTT CTGGCCTTTGCTGTGGTCCCCCTCTACCTCTGGGGCACTCGTGGAGCCACAAACCCTGGTTTGAAGTCT CTGACCCGGGAGGCCCTGGTGCTGCTCCCTGGGGCAGCCCTCACAGCAGCGGTGTTTGTGGCCACGGAC AGCTGGTATTTCTCCAGCCCCGCTACATCCAGGAACCTTGTCCTGACACCTGTCAACTTCTTGCACTAC AACCTGAATCCCCAAAACCTGGCGAGACATGGCACGCACGCGCGGCTCACTCACCTGGCAGTCAACGGC TTCCTGCTCTTCGGGGTGCTGCATGCCCAGGCCCTGCAGGCTGCGTGGCAACGGCTGCAAGTCGGCCTC CAGGCCTCTGCACAAATGGGCCTCCTGAGGGCACTGGGTGCCCGGAGCCTGCTGTCCAGCCCCAGGTCC TATCTCCTTCTCCTCTACTTCATGCCTCTGGCCCTGCTATCTGCCTTTAGCCACCAGGAGGCTCGGTTC CTGATTCCCCTCCTGGTCCCCCTGGTCCTGCTTTGTAGTCCACAGACGCAGCCTGTGCCTTGGAAGGGC ACTGTGGTCCTCTTCAACGCCCTCGGTGCCCTCCTCTTCGGCTGCCTGCATCAGGGGGGCCTGGTGCCT GGCCTGGAGTACCTGGAGCAGGTGGTCCATGCCCCTGTGCTCCCAAGCACACCCACCCACTACACACTC CTCTTCACTCACACCTACATGCCCCCCCGGCACCTCCTACACCTCCCAGGCCTGGGGGCACCAGTGGAG GTGGTGGACATGGGGGGGACTGAGGACTGGGCCCTGTGCCAAACCCTGAAAAGCTTCACCAGACAACCA GCCTGCCAAGTGGCTGGTGGGCCATGGCTCTGCCGCCTCTTTGTGGTAACCCCTGGCACCACCAGGCGT GCCGTGGAGAAGTGCAGCTTCCCCTTCAAGAATGAAACACTTTTATTTCCCCATCTGACCCTGGAGGAT CCACCAGCCCTGTCCTCCTTGCTGAGTGGGGCTTGGAGGGACCACCTCAGTCTTCACATTGTGGAGCTG GGGGAAGAAACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_025163 |
Insert Size | 1740 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_025163.3 |
RefSeq Size | 2759 bp |
RefSeq ORF | 1740 bp |
Locus ID | 80235 |
UniProt ID | Q86VD9 |
Protein Families | Transmembrane |
Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis |
MW | 63.5 kDa |
Gene Summary | The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. This gene encodes a protein that is localized to the endoplasmic reticulum, and is involved in GPI anchor biosynthesis. As shown for the yeast homolog, which is a member of a family of dolichol-phosphate-mannose (Dol-P-Man)-dependent mannosyltransferases, this protein can also add a side-branching fourth mannose to GPI precursors during the assembly of GPI anchors. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC208313 | PIGZ (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class Z (PIGZ) |
CNY 4,312.00 |
|
RC208313L3 | Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class Z (PIGZ), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC208313L4 | Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class Z (PIGZ), mGFP tagged |
CNY 5,890.00 |
|
RG208313 | PIGZ (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class Z (PIGZ) |
CNY 4,370.00 |