PIGZ (NM_025163) Human Untagged Clone

CAT#: SC305240

PIGZ (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class Z (PIGZ)



  "NM_025163" in other vectors (4)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-PIGZ Antibody
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GPI-MT-IV; PIG-Z; SMP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC305240 representing NM_025163.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCAGATCTGTGGATCCAGCGTAGCATCTGTAGCAGCTGGGACATCATTCCAGGTTTTGGGCCCGGTG
TGTTGGCAACAACTGGATCTGAAGATGGCAGTCAGGGTGCTTTGGGGTGGTCTCAGCCTGCTCCGAGTG
CTGTGGTGTCTCCTTCCGCAGACGGGCTATGTGCACCCAGATGAGTTCTTCCAGTCCCCTGAGGTGATG
GCAGAGGACATCCTGGGCGTTCAGGCCGCGCGGCCCTGGGAGTTTTACCCCAGCAGCTCCTGCCGCTCG
GTGCTCTTCCCCCTGCTGATCTCTGGTTCCACCTTCTGGCTGCTCAGGCTCTGGGAGGAGCTGGGGCCG
TGGCCTGGCCTGGTGAGCGGCTATGCGCTGCTGGTGGGGCCTCGACTCCTCCTCACTGCCCTTTCCTTT
GCTCTGGACGGGGCCGTGTACCACCTGGCCCCGCCGATGGGGGCGGATCGCTGGAACGCCCTGGCCCTG
CTGTCTGGTTCCTACGTCACCCTGGTCTTCTACACAAGGACCTTCTCCAACACCATTGAGGGACTCCTC
TTCACGTGGCTGCTGGTGCTGGTATCCTCCCATGTAACGTGGGGCCCTACACGCAAGGAGCCGGCGCCG
GGTCCACGGTGGCGCAGCTGGCTTCTTGGAGGCATTGTGGCTGCTGGCTTCTTCAACCGGCCCACCTTT
CTGGCCTTTGCTGTGGTCCCCCTCTACCTCTGGGGCACTCGTGGAGCCACAAACCCTGGTTTGAAGTCT
CTGACCCGGGAGGCCCTGGTGCTGCTCCCTGGGGCAGCCCTCACAGCAGCGGTGTTTGTGGCCACGGAC
AGCTGGTATTTCTCCAGCCCCGCTACATCCAGGAACCTTGTCCTGACACCTGTCAACTTCTTGCACTAC
AACCTGAATCCCCAAAACCTGGCGAGACATGGCACGCACGCGCGGCTCACTCACCTGGCAGTCAACGGC
TTCCTGCTCTTCGGGGTGCTGCATGCCCAGGCCCTGCAGGCTGCGTGGCAACGGCTGCAAGTCGGCCTC
CAGGCCTCTGCACAAATGGGCCTCCTGAGGGCACTGGGTGCCCGGAGCCTGCTGTCCAGCCCCAGGTCC
TATCTCCTTCTCCTCTACTTCATGCCTCTGGCCCTGCTATCTGCCTTTAGCCACCAGGAGGCTCGGTTC
CTGATTCCCCTCCTGGTCCCCCTGGTCCTGCTTTGTAGTCCACAGACGCAGCCTGTGCCTTGGAAGGGC
ACTGTGGTCCTCTTCAACGCCCTCGGTGCCCTCCTCTTCGGCTGCCTGCATCAGGGGGGCCTGGTGCCT
GGCCTGGAGTACCTGGAGCAGGTGGTCCATGCCCCTGTGCTCCCAAGCACACCCACCCACTACACACTC
CTCTTCACTCACACCTACATGCCCCCCCGGCACCTCCTACACCTCCCAGGCCTGGGGGCACCAGTGGAG
GTGGTGGACATGGGGGGGACTGAGGACTGGGCCCTGTGCCAAACCCTGAAAAGCTTCACCAGACAACCA
GCCTGCCAAGTGGCTGGTGGGCCATGGCTCTGCCGCCTCTTTGTGGTAACCCCTGGCACCACCAGGCGT
GCCGTGGAGAAGTGCAGCTTCCCCTTCAAGAATGAAACACTTTTATTTCCCCATCTGACCCTGGAGGAT
CCACCAGCCCTGTCCTCCTTGCTGAGTGGGGCTTGGAGGGACCACCTCAGTCTTCACATTGTGGAGCTG
GGGGAAGAAACCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_025163
Insert Size 1740 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_025163.3
RefSeq Size 2759 bp
RefSeq ORF 1740 bp
Locus ID 80235
UniProt ID Q86VD9
Protein Families Transmembrane
Protein Pathways Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
MW 63.5 kDa
Gene Summary The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. This gene encodes a protein that is localized to the endoplasmic reticulum, and is involved in GPI anchor biosynthesis. As shown for the yeast homolog, which is a member of a family of dolichol-phosphate-mannose (Dol-P-Man)-dependent mannosyltransferases, this protein can also add a side-branching fourth mannose to GPI precursors during the assembly of GPI anchors. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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