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Desmocollin 3 (DSC3) (NM_024423) Human Untagged Clone
CAT＃: SC305115
DSC3 (untagged)-Human desmocollin 3 (DSC3), transcript variant Dsc3b
CNY 16,250.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDHF3; DSC; DSC1; DSC2; DSC4; HT-CP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC305115 representing NM_024423.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCGCCGCTGGGCCCCGGCGCTCCGTGCGCGGAGCCGTCTGCCTGCATCTGCTGCTGACCCTCGTG ATCTTCAGTCGTGCTGGTGAAGCCTGCAAAAAGGTGATACTTAATGTACCTTCTAAACTAGAGGCAGAC AAAATAATTGGCAGAGTTAATTTGGAAGAGTGCTTCAGGTCTGCAGACCTCATCCGGTCAAGTGATCCT GATTTCAGAGTTCTAAATGATGGGTCAGTGTACACAGCCAGGGCTGTTGCGCTGTCTGATAAGAAAAGA TCATTTACCATATGGCTTTCTGACAAAAGGAAACAGACACAGAAAGAGGTTACTGTGCTGCTAGAACAT CAGAAGAAGGTATCGAAGACAAGACACACTAGAGAAACTGTTCTCAGGCGTGCCAAGAGGAGATGGGCA CCTATTCCTTGCTCTATGCAAGAGAATTCCTTGGGCCCTTTCCCATTGTTTCTTCAACAAGTTGAATCT GATGCAGCACAGAACTATACTGTCTTCTACTCAATAAGTGGACGTGGAGTTGATAAAGAACCTTTAAAT TTGTTTTATATAGAAAGAGACACTGGAAATCTATTTTGCACTCGGCCTGTGGATCGTGAAGAATATGAT GTTTTTGATTTGATTGCTTATGCGTCAACTGCAGATGGATATTCAGCAGATCTGCCCCTCCCACTACCC ATCAGGGTAGAGGATGAAAATGACAACCACCCTGTTTTCACAGAAGCAATTTATAATTTTGAAGTTTTG GAAAGTAGTAGACCTGGTACTACAGTGGGGGTGGTTTGTGCCACAGACAGAGATGAACCGGACACAATG CATACGCGCCTGAAATACAGCATTTTGCAGCAGACACCAAGGTCACCTGGGCTCTTTTCTGTGCATCCC AGCACAGGCGTAATCACCACAGTCTCTCATTATTTGGACAGAGAGGTTGTAGACAAGTACTCATTGATA ATGAAAGTACAAGACATGGATGGCCAGTTTTTTGGATTGATAGGCACATCAACTTGTATCATAACAGTA ACAGATTCAAATGATAATGCACCCACTTTCAGACAAAATGCTTATGAAGCATTTGTAGAGGAAAATGCA TTCAATGTGGAAATCTTACGAATACCTATAGAAGATAAGGATTTAATTAACACTGCCAATTGGAGAGTC AATTTTACCATTTTAAAGGGAAATGAAAATGGACATTTCAAAATCAGCACAGACAAAGAAACTAATGAA GGTGTTCTTTCTGTTGTAAAGCCACTGAATTATGAAGAAAACCGTCAAGTGAACCTGGAAATTGGAGTA AACAATGAAGCGCCATTTGCTAGAGATATTCCCAGAGTGACAGCCTTGAACAGAGCCTTGGTTACAGTT CATGTGAGGGATCTGGATGAGGGGCCTGAATGCACTCCTGCAGCCCAATATGTGCGGATTAAAGAAAAC TTAGCAGTGGGGTCAAAGATCAACGGCTATAAGGCATATGACCCCGAAAATAGAAATGGCAATGGTTTA AGGTACAAAAAATTGCATGATCCTAAAGGTTGGATCACCATTGATGAAATTTCAGGGTCAATCATAACT TCCAAAATCCTGGATAGGGAGGTTGAAACTCCCAAAAATGAGTTGTATAATATTACAGTCCTGGCAATA GACAAAGATGATAGATCATGTACTGGAACACTTGCTGTGAACATTGAAGATGTAAATGATAATCCACCA GAAATACTTCAAGAATATGTAGTCATTTGCAAACCAAAAATGGGGTATACCGACATTTTAGCTGTTGAT CCTGATGAACCTGTCCATGGAGCTCCATTTTATTTCAGTTTGCCCAATACTTCTCCAGAAATCAGTAGA CTGTGGAGCCTCACCAAAGTTAATGATACAGCTGCCCGTCTTTCATATCAGAAAAATGCTGGATTTCAA GAATATACCATTCCTATTACTGTAAAAGACAGGGCCGGCCAAGCTGCAACAAAATTATTGAGAGTTAAT CTGTGTGAATGTACTCATCCAACTCAGTGTCGTGCGACTTCAAGGAGTACAGGAGTAATACTTGGAAAA TGGGCAATCCTTGCAATATTACTGGGTATAGCACTGCTCTTTTCTGTATTGCTAACTTTAGTATGTGGA GTTTTTGGTGCAACTAAAGGGAAACGTTTTCCTGAAGATTTAGCACAGCAAAACTTAATTATATCAAAC ACAGAAGCACCTGGAGACGATAGAGTGTGCTCTGCCAATGGATTTATGACCCAAACTACCAACAACTCT AGCCAAGGTTTTTGTGGTACTATGGGATCAGGAATGAAAAATGGAGGGCAGGAAACCATTGAAATGATG AAAGGAGGAAACCAGACCTTGGAATCCTGCCGGGGGGCTGGGCATCATCATACCCTGGACTCCTGCAGG GGAGGACACACGGAGGTGGACAACTGCAGATACACTTACTCGGAGTGGCACAGTTTTACTCAACCCCGT CTCGGTGAAGAATCCATTAGAGGACACACTGGTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_024423 |
| Insert Size | 2520 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024423.3 |
| RefSeq Size | 7699 bp |
| RefSeq ORF | 2520 bp |
| Locus ID | 1825 |
| UniProt ID | Q14574 |
| Protein Families | Transmembrane |
| MW | 93.5 kDa |
| Gene Summary | The protein encoded by this gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Mutations in this gene are a cause of hypotrichosis and recurrent skin vesicles disorder. The protein can act as an autoantigen in pemphigus diseases, and it is also considered to be a biomarker for some cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014] Transcript Variant: This variant (Dsc3b) includes an additional exon in the 3' region and it thus differs in its 3' coding region and 3' UTR, compared to variant Dsc3a. The encoded isoform (Dsc3b) has a distinct C-terminus and is shorter than isoform Dsc3a. There are no publicly available full-length transcripts representing this variant, but it is supported by mRNA annotation on DNA accession AF293359.1, and by data in PMIDs 7929347 and 11027496. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214959 | DSC3 (Myc-DDK-tagged)-Human desmocollin 3 (DSC3), transcript variant Dsc3b |
CNY 8,840.00 |
|
| RC214959L3 | Lenti-ORF clone of DSC3 (Myc-DDK-tagged)-Human desmocollin 3 (DSC3), transcript variant Dsc3b |
CNY 10,740.00 |
|
| RC214959L4 | Lenti-ORF clone of DSC3 (mGFP-tagged)-Human desmocollin 3 (DSC3), transcript variant Dsc3b |
CNY 10,740.00 |
|
| RG214959 | DSC3 (tGFP-tagged) - Human desmocollin 3 (DSC3), transcript variant Dsc3b |
CNY 9,690.00 |
