NDST4 (NM_022569) Human Untagged Clone

CAT#: SC305035

NDST4 (untagged)-Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4 (NDST4)



  "NM_022569" in other vectors (4)

CNY 16,820.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-NDST4 Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms N-HSST; N-HSST 4; NDST-4; NHSST4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC305035 representing NM_022569.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAATCTTATTGTGAAACTTCGGAGAAGTTTTCGAACATTGATTGTTCTCTTAGCTACCTTTTGCTTG
GTGAGCATTGTCATTTCTGCCTATTTTCTCTACTCTGGCTACAAACAGGAAATGACACTTATTGAAACC
ACTGCAGAAGCAGAATGCACTGACATCAAAATTCTACCATATAGGTCAATGGAGCTGAAAACAGTTAAA
CCTATTGACACATCCAAAACGGACCCTACTGTCCTTCTCTTCGTGGAGAGCCAATACTCTCAACTCGGT
CAAGATATCATAGCTATTTTGGAGTCCAGCCGATTTCAGTACCACATGGTTATTGCCCCTGGAAAGGGA
GATATACCTCCTCTTACAGATAATGGCAAAGGGAAATATACTTTAGTTATTTATGAAAATATTCTGAAG
TATGTCAGCATGGACTCATGGAATCGAGAGCTTTTAGAAAAATACTGTGTGGAATACAGTGTTAGTATA
ATCGGTTTTCATAAAGCCAATGAGAACAGCTTACCAAGTACACAATTAAAAGGCTTTCCGTTAAACCTT
TTCAATAATCTAGCTCTAAAGGACTGTTTTGTTAACCCTCAATCTCCTTTGCTGCATATTACCAAAGCC
CCCAAGGTTGAGAAAGGCCCTCTTCCTGGGGAAGACTGGACTATTTTCCAATATAATCATTCAACCTAC
CAGCCTGTACTTTTAACTGAGTTACAGACAGAAAAATCCCTGTCATCCTTGTCTAGCAAAACACTCTTT
GCAACGGTGATTCAGGATCTGGGGCTTCATGATGGAATTCAGAGAGTACTTTTTGGCAACAACTTGAAC
TTTTGGCTGCACAAGCTCATCTTCATAGATGCCATCTCCTTCTTGTCAGGGAAGAGGCTGACATTGTCC
TTGGACAGGTACATCCTTGTGGACATTGATGATATATTTGTTGGGAAAGAGGGAACAAGGATGAATGTC
AAAGATGTGAAGGCATTACTAGAGACTCAAAATTTACTGCGCACTCAGGTTGCAAATTTTACCTTCAAC
CTTGGATTTTCAGGGAAGTTTTACCACACAGGGACTGAAGAGGAAGATGAAGGAGATGACCTTTTACTT
CGGTCTGTGGATGAGTTCTGGTGGTTTCCTCACATGTGGAGCCACATGCAGCCCCACCTCTTCCACAAC
GAGTCATCTTTAGTAGAGCAGATGATTCTCAACAAGGAATTTGCACTGGAACATGGAATACCAATCAAC
ATGGGCTATGCTGTGGCCCCACATCACTCAGGGGTCTACCCGGTTCACATTCAGCTGTATGCAGCTTGG
AAGAAGGTCTGGGGTATTCAAGTCACCAGCACTGAAGAATATCCACATCTGAAACCTGCCCGGTACAGA
AAGGGCTTCATTCACAATAGCATCATGGTCCTCCCTCGACAGACTTGTGGGTTGTTCACTCACACTATT
TTCTACAAAGAATATCCAGGAGGACCCCAAGAACTGGATAAAAGTATCAGAGGAGGTGAACTTTTTCTC
ACAATCCTTCTAAACCCAATCAGCATTTTCATGACCCATTTATCAAACTATGGAAATGACCGCCTAGGG
TTATATACCTTTGTGAACTTGGTCAACTTTGTGCAGAGCTGGACCAACCTGAAATTGCAAACTCTGCCT
CCAGTGCAACTGGCCCACCAGTATTTTGAGCTCTTCCCTGAGCAGAAAGACCCTCTATGGCAGAATCCA
TGTGATGATAAACGACACAAAGACATCTGGTCCAGAGAGAAAACTTGTGACCACTTACCAAAATTTCTA
GTAATTGGTCCACAAAAAACAGGAACAACTGCACTTTATTTATTTCTTCTTATGCATCCTTCAATCATC
AGCAATCTCCCTAGTCCAAAGACATTTGAGGAAGTTCAGTTCTTTAATGGCAACAACTATCACAAAGGA
ATAGACTGGTATATGGACTTTTTCCCTACACCATCCAATACTACAAGTGACTTTCTGTTTGAAAAGAGT
GCTAATTACTTCCATTCGGAAGAAGCTCCAAGACGAGCCGCATCTCTTGTCCCCAAAGCCAAGATCATC
ACCATCCTCATTGACCCCTCAGACAGGGCATACTCTTGGTACCAGCACCAACGATCACATGAAGATCCA
GCTGCTCTGAGGTTCAATTTCTATGAAGTTATTTCAACAGGACATTGGGCTCCATCTGACTTAAAAACT
TTGCAGAGAAGATGCCTAGTACCTGGATGGTATGCAGTCCACATAGAAAGATGGCTAACTTACTTTGCT
ACTTCTCAGTTGCTAATTATTGATGGACAGCAGCTGAGATCTGACCCAGCTACTGTGATGGATGAAGTC
CAGAAGTTTCTGGGAGTTACACCTCGTTATAATTACTCTGAAGCACTAACGTTTGATCCCCAAAAGGGT
TTTTGGTGTCAATTACTGGAAGGAGGAAAGACAAAATGCCTTGGAAAAAGCAAAGGCCGAAAATATCCA
CCTATGGATCCAGAGTCTAGAACGTTCCTCTCTAATTACTACCGAGATCATAATGTGGAACTATCCAAA
CTGCTACACAGACTGGGACAGCCTCTGCCATCGTGGCTGAGACAGGAACTGCAGAAAGTGAGATAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_022569
Insert Size 2619 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_022569.2
RefSeq Size 3341 bp
RefSeq ORF 2619 bp
Locus ID 64579
UniProt ID Q9H3R1
Protein Families Transmembrane
Protein Pathways Heparan sulfate biosynthesis, Metabolic pathways
MW 100.7 kDa
Gene Summary Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has low deacetylase activity but high sulfotransferase activity (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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