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HERC4 (NM_022079) Human Untagged Clone
CAT＃: SC304987
HERC4 (untagged)-Human hect domain and RLD 4 (HERC4), transcript variant 1
CNY 7,368.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC304987 representing NM_022079.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTGTGCTGGGGAAATGCATCCTTTGGGCAGCTAGGTTTGGGTGGAATTGATGAAGAAATTGTACTA GAGCCCAGAAAAAGTGACTTCTTTATAAATAAAAGGGTCCGAGATGTAGGATGTGGACTCAGACATACT GTGTTTGTTCTGGATGATGGAACAGTGTACACATGTGGATGTAATGATCTAGGACAGCTAGGTCATGAA AAATCCAGAAAGAAACCAGAGCAGGTTGTTGCCCTGGATGCCCAAAATATTGTAGCTGTTTCATGTGGA GAAGCTCATACGTTAGCGCTAAATGACAAAGGCCAGGTGTATGCTTGGGGTCTCGATTCTGATGGACAG CTTGGCCTGGTAGGATCAGAGGAATGCATCAGAGTACCCAGAAATATTAAAAGTTTGTCAGATATCCAG ATTGTACAGGTTGCTTGTGGTTACTATCATTCACTTGCACTTTCTAAAGCAAGTGAAGTCTTCTGTTGG GGACAGAATAAATATGGCCAATTGGGTTTAGGTACTGACTGTAAAAAGCAAACTTCACCGCAGCTGCTT AAGTCTTTGCTTGGAATCCCTTTCATGCAAGTTGCAGCAGGAGGAGCCCATAGTTTTGTACTCACCCTT TCTGGAGCTATCTTTGGATGGGGACGCAACAAGTTTGGTCAGCTAGGTCTTAATGATGAAAATGATAGG TATGTTCCTAATTTACTAAAGTCACTAAGATCTCAGAAAATAGTTTATATTTGTTGTGGAGAAGATCAT ACTGCTGCTCTAACCAAGGAAGGTGGAGTGTTTACTTTTGGAGCTGGAGGGTATGGTCAGTTGGGCCAT AATTCTACCAGTCATGAAATAAACCCAAGGAAAGTTTTTGAACTTATGGGAAGCATTGTCACTGAGATT GCTTGTGGACGGCAGCACACTTCTGCTTTTGTTCCTTCATCAGGACGAATTTACTCTTTTGGGCTTGGT GGTAATGGGCAGCTGGGAACCGGTTCAACAAGCAACAGGAAAAGCCCCTTTACTGTAAAAGGAAATTGG TACCCCTATAATGGGCAGTGTCTACCAGATATTGATTCTGAAGAATATTTCTGTGTAAAAAGAATTTTC TCAGGGGGAGATCAAAGCTTTTCACATTACTCTAGTCCCCAGAACTGTGGGCCACCAGATGACTTCAGA TGTCCCAATCCGACAAAGCAGATCTGGACAGTGAATGAAGCTCTAATTCAGAAATGGCTGAGCTATCCT TCTGGAAGGTTTCCTGTGGAGATAGCCAATGAGATAGATGGAACGTTTTCTTCCTCTGGTTGCCTAAAT GGAAGTTTTTTAGCTGTTAGCAATGATGATCACTATAGAACAGGTACCAGATTTTCAGGGGTTGATATG AATGCTGCTAGGCTTTTATTCCACAAACTTATACAACCTGATCATCCGCAGATATCTCAGCAGGTGGCA GCTAGTTTGGAAAAGAATCTTATTCCTAAACTGACTAGCTCCTTACCTGATGTTGAAGCATTGAGGTTT TATCTTACTCTACCAGAATGTCCCCTGATGAGTGATTCCAACAATTTCACAACAATAGCAATTCCCTTT GGTACAGCTCTTGTGAACCTAGAAAAGGCACCACTGAAAGTACTTGAAAACTGGTGGTCAGTACTTGAA CCTCCACTATTCCTCAAGATAGTAGAACTTTTTAAGGAAGTTGTGGTACATCTTTTGAAACTCTACAAG ATCGGTATTCCCCCTTCTGAAAGAAGAATTTTCAACAGTTTTCTTCATACTGCATTAAAGGTTTTAGAA ATACTACATAGGGTAAATGAGAAAATGGGACAGATTATACAGTATGATAAATTTTATATACATGAAGTA CAAGAATTGATAGACATAAGAAATGATTATATCAACTGGGTCCAACAGCAGGCCTATGGAATGGATGTC AACCATGGATTAACTGAGTTGGCAGATATCCCTGTTACAATCTGTACATATCCATTTGTATTTGATGCC CAAGCAAAAACTACTCTGTTACAGACCGATGCAGTCTTACAGATGCAGATGGCTATTGATCAGGCCCAC AGGCAGAATGTCTCCTCTCTTTTTCTCCCAGTGATTGAATCTGTGAATCCCTGCTTAATTCTAGTGGTG CGTAGAGAAAATATTGTAGGAGATGCAATGGAAGTCCTTAGGAAAACAAAGAACATAGATTACAAGAAG CCACTCAAGGTTATATTTGTTGGAGAAGATGCTGTGGATGCAGGAGGGGTGCGCAAAGAATTTTTCTTG CTCATCATGAGGGAATTATTGGATCCTAAATACGGCATGTTTAGGTATTATGAAGATTCCAGGCTCATT TGGTTTTCTGATAAGACATTTGAAGACAGTGATTTGTTCCATTTGATTGGTGTTATCTGTGGCTTAGCA ATTTATAATTGTACCATTGTGGACCTCCATTTTCCTTTGGCTTTATATAAGAAACTACTGAAAAAGAAG CCATCCTTGGATGATTTGAAAGAACTAATGCCTGATGTTGGGAGAAGCATGCAACAGTTACTGGATTAT CCAGAAGATGACATAGAGGAAACATTTTGTCTTAATTTTACGATCACAGTTGAAAACTTTGGTGCAACA GAAGTGAAAGAGCTGGTTCTAAATGGTGCAGACACAGCTGTTAACAAACAAAATCGGCAAGAGTTTGTC GATGCTTATGTGGATTACATATTCAATAAATCAGTGGCTTCCTTATTTGATGCTTTTCATGCGGGCTTT CATAAGGTCTGTGGAGGAAAAGTCCTTCTGCTCTTTCAGCCTAATGAACTACAAGCAATGGTCATTGGA AATACAAATTATGATTGGAAGGAACTGGAAAAGAATACAGAATACAAAGGGGAATATTGGGCAGAACAT CCTACGATAAAAATTTTTTGGGAAGTATTTCACGAATTACCATTGGAAAAGAAGAAACAGTTTCTGTTA TTTTTGACAGGTAGTGATCGCATTCCTATTCTTGGTATGAAGAGTCTGAAACTAGTCATCCAGTCCACA GGAGGTGGTGAGGAGTATCTCCCAGTTTCCCATACTTGTTTTAATCTTCTGGATCTTCCAAAATATACA GAAAAAGAAACTCTACGCTCTAAACTGATCCAAGCTATTGATCACAATGAAGGCTTCAGTTTAATATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_022079 |
| Insert Size | 3174 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022079.1 |
| RefSeq Size | 4531 bp |
| RefSeq ORF | 3174 bp |
| Locus ID | 26091 |
| UniProt ID | Q5GLZ8 |
| Protein Families | Druggable Genome |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 118.6 kDa |
| Gene Summary | HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211865 | HERC4 (Myc-DDK-tagged)-Human hect domain and RLD 4 (HERC4), transcript variant 1 |
CNY 7,360.00 |
|
| RC211865L3 | Lenti ORF clone of Human hect domain and RLD 4 (HERC4), transcript variant 1, Myc-DDK-tagged |
CNY 12,450.00 |
|
| RC211865L4 | Lenti ORF clone of Human hect domain and RLD 4 (HERC4), transcript variant 1, mGFP tagged |
CNY 12,450.00 |
|
| RG211865 | HERC4 (tGFP-tagged) - Human hect domain and RLD 4 (HERC4), transcript variant 1 |
CNY 11,590.00 |
